Identification, Mapping, and Molecular Marker Development for Rgsr8.1: A New Quantitative Trait Locus Conferring Resistance to Gibberella Stalk Rot in Maize (Zea mays L.)

Maize stalk rot is a major fungal disease worldwide, and is difficult to control by chemical methods. Therefore, in maize breeding, quantitative trait loci (QTLs) conferring resistance are important for controlling the disease. Next-generation sequencing technologies are considered a rapid and efficient method to establish the association of agronomic traits with molecular markers or candidate genes. In the present study, we employed QTL-seq, which is a whole-genome resequencing-based approach, to identify candidate genomic regions conferring resistance to maize stalk rot. A novel resistance QTL Rgsr8.1 was finely mapped, conferring broad-spectrum resistance to Gibberella stalk rot (GSR). Segregation analysis in F2 and BC1F1 populations, which were derived from a cross between 18327 (Susceptible) and S72356 (Resistant), indicated that the resistance to GSR was likely to be a quantitatively inherited trait in maize. The result of QTL-seq showed that the resistance to GSR was mapped on chromosome 8 from 161.001 to 170.6 Mb. Based on the simple sequence repeat (SSR) markers, single-nucleotide polymorphism (SNP) markers, and the recombinant test, the location of Rgsr8.1 was narrowed down to 2.04 Mb, flanked by SSR-65 and SNP-25 markers at the physical location from 164.69 to 166.72 Mb based on the maize reference genome. In this region, two candidate resistant genes were found with, one auxin-responsive elements and the other encoding a disease resistance protein. In summary, these results will be useful in maize breeding programs to improve the resistance to GSR in maize.

• Publication year

  2017

• Venue

  Frontiers in Plant Science

• Publication date

  2017-08-03

• Fields of study

  Agricultural and Food Sciences, Medicine, Biology

• Identifiers
  DOI 10.3389/fpls.2017.01355PMID 28824686PMCID 5540892
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Two candidate resistance genes were identified in the narrowed Rgsr8.1 interval, including one associated with auxin response and one encoding a disease resistance protein.

  Confidence 0.90

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review
• Rgsr8.1 is a novel resistance QTL conferring broad-spectrum resistance to Gibberella stalk rot in maize.

  Confidence 0.96

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review
• QTL-seq mapped the resistance locus to chromosome 8 and the interval was refined to a 2.04 Mb region flanked by SSR-65 and SNP-25 markers.

  Confidence 0.97

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review
• Segregation in the F2 and BC1F1 populations indicates that resistance to Gibberella stalk rot is a quantitatively inherited trait in maize.

  Confidence 0.93

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review

• bc1f1 population

  population

  The first backcross generation used alongside the F2 population to analyze inheritance of the resistance trait.

  Aliases: BC1F1

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review
• candidate resistant genes

  genes

  Genes located within the final mapped interval that were examined as possible contributors to resistance.

  Aliases: candidate genes

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review
• f2 population

  population

  The second filial generation derived from the cross between susceptible and resistant parents for segregation analysis.

  Aliases: F2

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review
• gibberella stalk rot

  disease

  A fungal stalk rot disease of maize used here as the target resistance trait.

  Aliases: GSR, maize stalk rot

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review
• maize

  crop

  The crop species Zea mays used as the host plant for mapping resistance to stalk rot.

  Aliases: Zea mays

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review
• qtl-seq

  method

  A whole-genome resequencing-based approach used to locate genomic regions associated with a trait by comparing pooled individuals with contrasting phenotypes.

  Aliases: quantitative trait locus sequencing

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review
• rgsr8.1

  locus

  A resistance quantitative trait locus on maize chromosome 8 that is the mapped genomic interval investigated in this work.

  Aliases: Rgsr8.1 locus

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review
• snp markers

  markers

  Single-nucleotide polymorphism markers used to narrow the resistance interval on the maize genome.

  Aliases: single-nucleotide polymorphism markers

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review
• ssr markers

  markers

  Simple sequence repeat markers used to genotype the mapping population and refine the locus interval.

  Aliases: simple sequence repeat markers, microsatellite markers

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) reviewjihoonc (k5vuy3tzcm) review

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