Accurate Estimation of Viral Abundance by Epifluorescence Microscopy

No abstract is available for this paper.

• Publication year

  2004

• Venue

  Applied and Environmental Microbiology

• Publication date

  2004-07-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1128/AEM.70.7.3862-3867.2004PMID 15240256PMCID PMC444834
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Factors influencing the loss of bacteria in preserved seawater samples

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• Optimization of Procedures for Counting Viruses by Flow Cytometry

  2004influential reference
• Viral and bacterial production in the North Water: in situ measurements, batch-culture experiments and characterization and distribution of a virus host system

  2002cited by this paper
• Determination of Virus Abundance in Marine Sediments

  2001cited by this paper
• Application of Digital Image Analysis and Flow Cytometry To Enumerate Marine Viruses Stained with SYBR Gold

  2001influential reference
• Dynamic bacterial and viral response to an algal bloom at subzero temperatures

  2001cited by this paper
• A Comparison of Methods for Counting Viruses in Aquatic Systems

  2000cited by this paper
• Flow cytometric detection of viruses.

  2000influential reference
• Virioplankton: Viruses in Aquatic Ecosystems

  2000cited by this paper
• Marine viruses and their biogeochemical and ecological effects

  1999cited by this paper
• Viruses and Nutrient Cycles in the Sea Viruses play critical roles in the structure and function of aquatic food webs

  1999cited by this paper
• Enumeration of Marine Viruses in Culture and Natural Samples by Flow Cytometry

  1999influential reference
• Use of SYBR Green I for rapid epifluorescence counts of marine viruses and bacteria

  1998influential reference
• Virus à la sauce Yo‐Pro: Microwave enhanced staining for counting viruses by epifluorescence microscopy

  1997influential reference
• Comparison of epifluorescence and transmission electron microscopy for counting viruses in natural marine waters

  1997cited by this paper
• Direct counts of viruses in natural waters and laboratory cultures by epifluorescence microscopy

  1996cited by this paper
• The significance of viruses to mortality in aquatic microbial communities

  1994cited by this paper
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  1992cited by this paper
• Abundance of Viruses in Marine Waters: Assessment by Epifluorescence and Transmission Electron Microscopy

  1991cited by this paper
• Infection of phytoplankton by viruses and reduction of primary productivity

  1990cited by this paper
• Enumeration and biomass estimation of planktonic bacteria and viruses by transmission electron microscopy

  1990cited by this paper
• Viral mortality of marine bacteria and cyanobacteria

  1990cited by this paper
• High abundance of viruses found in aquatic environments

  1989cited by this paper
• Direct Acridine Orange Counting of Bacteria Preserved with Acidified Lugol Iodine

  1986cited by this paper
• The use of DAPI for identifying and counting aquatic microflora1

  1980cited by this paper

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