Progressive sheet-to-tubule transformation is a general mechanism for endoplasmic reticulum partitioning in dividing mammalian cells

During mitosis, ER network reorganization can lead to packing of the ER into tight concentric layers at the cell cortex and occurs in tandem with rounding of the cell. Morphometric and 3D EM analysis shows that in addition to reorganization, ER sheets undergo transformation toward more fenestrated and tubular forms before anaphase in mammalian cells.

• Publication year

  2012

• Venue

  Molecular Biology of the Cell

• Publication date

  2012-07-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1091/mbc.E10-12-0950PMID 22573885PMCID 3386207
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• A 3D analysis of yeast ER structure reveals how ER domains are organized by membrane curvature

  2011cited by this paper
• Formation of the postmitotic nuclear envelope from extended ER cisternae precedes nuclear pore assembly

  2011cited by this paper
• NCMIR methods for 3D EM: a new protocol for preparation of biological specimens for serial block face scanning electron microscopy

  2010cited by this paper
• Five Arabidopsis Reticulon Isoforms Share Endoplasmic Reticulum Location, Topology, and Membrane-Shaping Properties[W]

  2010cited by this paper
• ER sliding dynamics and ER–mitochondrial contacts occur on acetylated microtubules

  2010cited by this paper
• Mechanisms determining the morphology of the peripheral ER.

  2010influential reference
• Enhancement of Procollagen Biosynthesis by p180 through Augmented Ribosome Association on the Endoplasmic Reticulum in Response to Stimulated Secretion

  2010cited by this paper
• Cisternal organization of the endoplasmic reticulum during mitosis.

  2009influential reference
• Ultramicrotomy in the ESEM, a versatile method for materials and life sciences

  2009cited by this paper
• Knockdown of p180 eliminates the terminal differentiation of a secretory cell line.

  2008influential reference
• p180 is involved in the interaction between the endoplasmic reticulum and microtubules through a novel microtubule-binding and bundling domain.

  2007cited by this paper
• Reticulon 4a/NogoA locates to regions of high membrane curvature and may have a role in nuclear envelope growth

  2007cited by this paper
• Climp-63-mediated binding of microtubules to the ER affects the lateral mobility of translocon complexes

  2007cited by this paper
• Lamin B receptor plays a role in stimulating nuclear envelope production and targeting membrane vesicles to chromatin during nuclear envelope assembly through direct interaction with importin β

  2007cited by this paper
• Characterization and subcellular localization of human neutral class IIα-mannosidase cytosolic enzymes/free oligosaccharides/glycosidehydrolase family 38/M2C1/N-glycosylation

  2007cited by this paper
• Characterization and subcellular localization of human neutral class II alpha-mannosidase [corrected].

  2007cited by this paper
• Ribosomal Slowdown Mediates Translational Arrest during Cellular Division

  2007cited by this paper
• Endoplasmic reticulum remains continuous and undergoes sheet-to-tubule transformation during cell division in mammalian cells

  2007influential reference
• Rough sheets and smooth tubules.

  2006cited by this paper
• A class of membrane proteins shaping the tubular endoplasmic reticulum.

  2006cited by this paper
• Fluorophores for live cell imaging of AGT fusion proteins across the visible spectrum.

  2006cited by this paper
• NSF/SNAPs and p97/p47/VCIP135 are sequentially required for cell cycle‐dependent reformation of the ER network

  2005cited by this paper
• Involvement of the actin cytoskeleton and homotypic membrane fusion in ER dynamics in Caenorhabditis elegans.

  2005cited by this paper
• Translational control during mitosis.

  2005cited by this paper
• Cholesterol and steroid synthesizing smooth endoplasmic reticulum of adrenocortical cells contains high levels of proteins associated with the translocation channel.

  2005cited by this paper
• Endoplasmic reticulum positioning and partitioning in mitotic HeLa cells

  2005cited by this paper
• Serial Block-Face Scanning Electron Microscopy to Reconstruct Three-Dimensional Tissue Nanostructure

  2004cited by this paper
• Microtubule-associated smooth endoplasmic reticulum in the frog's brain

  2004cited by this paper
• Fenestrae in golgi and endoplasmic reticulum cisternae of human brain tumours

  2004cited by this paper
• Influence of aldehyde fixation on the morphology of endosomes and lysosomes: quantitative analysis and electron tomography

  2003cited by this paper
• Dynamics of the endoplasmic reticulum during early development of Drosophila melanogaster.

  2003cited by this paper
• Formation of stacked ER cisternae by low affinity protein interactions

  2003cited by this paper
• Active translocon complexes labeled with GFP–Dad1 diffuse slowly as large polysome arrays in the endoplasmic reticulum

  2002cited by this paper
• Golgi clusters and vesicles mediate mitotic inheritance independently of the endoplasmic reticulum

  2001influential reference
• Subdomain-Specific Localization of Climp-63 (P63) in the Endoplasmic Reticulum Is Mediated by Its Luminal α-Helical Segment

  2001cited by this paper
• Three dimensional configuration of the secretory pathway and segregation of secretion granules in the yeast Saccharomyces cerevisiae.

  2001cited by this paper
• Dynamics of the endoplasmic reticulum and golgi apparatus during early sea urchin development.

  2000cited by this paper
• Nuclear Membrane Dynamics and Reassembly in Living Cells: Targeting of an Inner Nuclear Membrane Protein in Interphase and Mitosis

  1997cited by this paper
• Computer visualization of three-dimensional image data using IMOD.

  1996cited by this paper
• Transport into and out of the Golgi complex studied by transfecting cells with cDNAs encoding horseradish peroxidase

  1994cited by this paper
• Membrane biogenesis during B cell differentiation: most endoplasmic reticulum proteins are expressed coordinately

  1990cited by this paper
• Cortical endoplasmic reticulum in plants

  1990cited by this paper
• Microtubules and the endoplasmic reticulum are highly interdependent structures

  1986cited by this paper
• Spindle-membrane associations in freeze fractured HeLa cells.

  1984cited by this paper
• Spindle-membrane associations in freeze fractured HeLa cells

  1984cited by this paper
• Transepithelial endoplasmic reticulum in rat proximal convoluted tubule.

  1983cited by this paper
• The structure of the endoplasmic reticulum revealed by osmium tetroxide-potassium ferricyanide staining.

  1981cited by this paper
• The structure of the endoplasmic reticulum revealed by osmium tetroxide-potassium ferricyanide staining.

  1981cited by this paper
• Fenestrae in the rough endoplasmic reticulum of Xenopus laevis hepatocytes

  1978cited by this paper
• FENESTRATIONS OF SARCOPLASMIC RETICULUM

  1973cited by this paper
• FENESTRAE IN THE ROUGH ENDOPLASMIC RETICULUM OF THE EXOCRINE PANCREATIC CELLS

  1972cited by this paper
• A comparative study of freeze-etch replicas and thin sections of rat liver.

  1971cited by this paper
• Regulation of protein synthesis in mammalian cells. II. Inhibition of protein synthesis at the level of initiation during mitosis.

  1970cited by this paper
• Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.

  1968cited by this paper
• THE ENDOPLASMIC RETICULUM

  1956cited by this paper

• PERK-dependent reciprocal crosstalk between ER and non-centrosomal microtubules coordinates ER architecture and cell shape

  2025cites this paper
• nERdy: network analysis of endoplasmic reticulum dynamics

  2025cites this paper
• Tubulation of membrane sheets by curvature-inducing proteins.

  2025cites this paper
• Mammalian Cells Integrate Endoplasmic Reticulum and Nuclear Envelope signals to time mitotic entry

  2025cites this paper
• Remodeling of ER Membrane Contact Sites During Cell Division

  2025cites this paper
• Mitotic spindle membranes

  2025cites this paper
• Nuclear envelope-associated lipid droplets are enriched in cholesteryl esters and increase during inflammatory signaling

  2025cites this paper
• Mitochondria-ER contact sites expand during mitosis

  2024cites this paper
• Dynamic remodelling of the endoplasmic reticulum for mitosis.

  2024cites this paper
• Unveiling the Dynamics: A Comprehensive Review of Biological Membrane Kinetics During Cell Division

  2024cites this paper
• Endoplasmic reticulum membrane remodeling by targeting reticulon-4 induces pyroptosis to facilitate antitumor immune

  2024cites this paper
• Organelle Communication with the Nucleus.

  2024cites this paper
• Cytoplasmic nucleoporin assemblage: the cellular artwork in physiology and disease

  2024cites this paper
• Aurora kinase A-mediated phosphorylation triggers structural alteration of Rab1A to enhance ER complexity during mitosis

  2024cites this paper
• Degradation of STIM1 through FAM134B-mediated ER-phagy is potentially involved in cell proliferation

  2024cites this paper
• Preserving Genome Integrity: Unveiling the Roles of ESCRT Machinery

  2024cites this paper
• Katanin, kinesin-13, and ataxin-2 inhibit premature interaction between maternal and paternal genomes in C. elegans zygotes

  2024cites this paper
• Sculpting nuclear envelope identity from the endoplasmic reticulum during the cell cycle

  2024cites this paper
• Katanin, kinesin-13 and ataxin-2 inhibit premature interaction between maternal and paternal genomes in C. elegans zygotes

  2024cites this paper
• The tubule-sheet continuum model links the pattern of nuclear envelope assembly to nuclear envelope integrity

  2024cites this paper
• Annexin A2 and lamin B join membrane recycling compartments for the assembly of biomolecular condensates operating in mitotic partitioning

  2024cites this paper
• Multiscale biophysical analysis of nucleolus disassembly during mitosis

  2024cites this paper
• A bird’s-eye view of the endoplasmic reticulum in filamentous fungi

  2024cites this paper
• Axonal endoplasmic reticulum tubules control local translation via P180/RRBP1-mediated ribosome interactions

  2024cites this paper
• Accelerating data sharing and reuse in volume electron microscopy

  2024cites this paper
• How host ER membrane chaperones and morphogenic proteins support virus infection.

  2023cites this paper
• A tubule-sheet continuum model for the mechanism of nuclear envelope assembly.

  2023cites this paper
• Neuronal endoplasmic reticulum architecture and roles in axonal physiology.

  2023cites this paper
• The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ

  2023cites this paper
• The Physical Biology of Nucleolus Disassembly

  2023cites this paper
• Dysregulation of the endoplasmic reticulum blocks recruitment of centrosome-associated proteins resulting in mitotic failure

  2023cites this paper
• The nanoscale organization of reticulon 4 shapes local endoplasmic reticulum structure in situ

  2023cites this paper
• Inverse Designing Surface Curvatures by Deep Learning

  2023cites this paper
• Sample preparation and data collection for serial block face scanning electron microscopy of mammalian cell monolayers

  2023cites this paper
• 3D Ultrastructural Visualization of Mitosis Fidelity in Human Cells Using Serial Block Face Scanning Electron Microscopy (SBF-SEM)

  2023cites this paper
• Axonal ER tubules regulate local translation via P180/RRBP1-mediated ribosome interactions

  2022cites this paper
• Morphology of Phagophore Precursors by Correlative Light-Electron Microscopy

  2022cites this paper
• Structural Diversity within the Endoplasmic Reticulum-From the Microscale to the Nanoscale.

  2022cites this paper
• The LEM-ESCRT toolkit: Repair and maintenance of the nucleus

  2022cites this paper
• EMC3 regulates mesenchymal cell survival via control of the mitotic spindle assembly

  2022cites this paper
• Volume electron microscopy

  2022cites this paper
• The mammalian rhomboid protein RHBDL4 protects against endoplasmic reticulum stress by regulating the morphology and distribution of ER sheets

  2022cites this paper
• ER Morphology in the Pathogenesis of Hereditary Spastic Paraplegia

  2021cites this paper
• PERK-dependent reciprocal crosstalk between ER and non-centrosomal microtubules coordinates ER architecture and cell shape

  2021cites this paper
• The stress-sensing domain of activated IRE1α forms helical filaments in narrow ER membrane tubes

  2021cites this paper
• A CTDNEP1-lipin 1-mTOR regulatory network restricts ER membrane biogenesis to enable chromosome motions necessary for mitotic fidelity

  2021cites this paper
• Generation of Tubular and Membranous Shape Textures with Curvature Functionals

  2021cites this paper
• Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes

  2021cites this paper
• Morphological Heterogeneity of the Endoplasmic Reticulum within Neurons and Its Implications in Neurodegeneration

  2021cites this paper
• Dynamic remodeling of presynaptic endoplasmic reticulum is coordinated through actin and microtubule crosstalk and contributes to defective stimulus-response in Spinal Muscular Atrophy

  2021cites this paper
• Hereditary Spastic Paraplegia and Future Therapeutic Directions: Beneficial Effects of Small Compounds Acting on Cellular Stress

  2021cites this paper
• Multiple roles for actin in secretory and endocytic pathways.

  2021cites this paper
• Inherited nuclear pore substructures template post-mitotic pore assembly.

  2021cites this paper
• RHBDL4 protects against endoplasmic reticulum stress by regulating the morphology and distribution of ER sheets

  2021cites this paper
• Mitotic disassembly and reassembly of nuclear pore complexes.

  2021cites this paper
• Recent developments in membrane curvature sensing and induction by proteins.

  2021cites this paper
• A novel automated image analysis pipeline for quantifying morphological changes to the endoplasmic reticulum in cultured human cells

  2021cites this paper
• The endoplasmic reticulum adopts two distinct forms of tubules

  2021influential citation
• Specific subdomain localization of ER resident proteins and membrane contact sites resolved by electron microscopy.

  2021cites this paper
• Dynamic remodeling of ribosomes and endoplasmic reticulum in axon terminals of motoneurons

  2021cites this paper
• The endoplasmic reticulum adopts two distinct tubule forms

  2021influential citation
• Morphomics via Next-generation Electron Microscopy

  2021cites this paper
• Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.

  2021cites this paper
• Herpesvirus Nuclear Egress across the Outer Nuclear Membrane

  2021cites this paper
• Reticulon-like REEP4 at the inner nuclear membrane promotes nuclear pore complex formation

  2021cites this paper
• Contact sites between endoplasmic reticulum sheets and mitochondria regulate mitochondrial DNA replication and segregation

  2021influential citation
• Identifying long-range synaptic inputs using genetically encoded labels and volume electron microscopy

  2021cites this paper
• Mechanism of shaping membrane nanostructures of endoplasmic reticulum

  2021cites this paper
• Zika Virus Induces Mitotic Catastrophe in Human Neural Progenitors by Triggering Unscheduled Mitotic Entry in the Presence of DNA Damage While Functionally Depleting Nuclear PNKP

  2021cites this paper
• Das endoplasmatische Retikulum in der Mitose — ein wandelbares Netz

  2020cites this paper
• Married at Birth: Regulation of Cellular Fat Metabolism by ER–Lipid Droplet Crosstalk

  2020cites this paper
• REEP4 is recruited to the inner nuclear membrane by ELYS and promotes nuclear pore complex formation

  2020cites this paper
• The coordination of nuclear envelope assembly and chromosome segregation in metazoans

  2020cites this paper
• Coronavirus infection induces progressive restructuring of the endoplasmic reticulum involving the formation and degradation of double membrane vesicles

  2020cites this paper
• Ice2 promotes ER membrane biogenesis in yeast by inhibiting the conserved lipin phosphatase complex

  2020cites this paper
• Endoplasmic reticulum contact sites regulate the dynamics of membraneless organelles

  2020cites this paper
• Membrane and organelle dynamics during cell division

  2020cites this paper
• 3D CLEM

  2019cites this paper
• Microtubules Stabilization by Mutant Spastin Affects ER Morphology and Ca2+ Handling

  2019cites this paper
• Functions and Mechanisms of the Human Ribosome-Translocon Complex.

  2019cites this paper
• Differential requirements of cyclase associated protein (CAP) for actin turnover during the lytic cycle of Toxoplasma gondii

  2019cites this paper
• REEP3 and REEP4 determine the tubular morphology of the endoplasmic reticulum during mitosis

  2019cites this paper
• Serial block face-scanning electron microscopy for volume electron microscopy.

  2019cites this paper
• Organelle Inheritance Control of Mitotic Entry and Progression: Implications for Tissue Homeostasis and Disease

  2019cites this paper
• Semi-Intact Cell System for Reconstituting and Analyzing Cellular Golgi Dynamics.

  2019cites this paper
• Geometric coupling of helicoidal ramps and curvature-inducing proteins in organelle membranes

  2019cites this paper
• 3D cellular reconstruction of cortical glia and parenchymal morphometric analysis from Serial Block-Face Electron Microscopy of juvenile rat.

  2019cites this paper
• Lipid species affect morphology of endoplasmic reticulum: a sea urchin oocyte model of reversible manipulation[S]

  2019cites this paper
• SBEM Techniques

  2019cites this paper
• Differential requirements for cyclase-associated protein (CAP) in actin-dependent processes of Toxoplasma gondii

  2019cites this paper
• Serial block face scanning electron microscopy in cell biology: Applications and technology.

  2019cites this paper
• Reconstituting the reticular ER network – mechanistic implications and open questions

  2019cites this paper
• Roles of Nup133, Nup153 and membrane fenestrations in assembly of the nuclear pore complex at the end of mitosis

  2019cites this paper
• Microtubules are necessary for proper Reticulon localization during mitosis

  2019cites this paper
• Investigating proteins that regulate the architecture of the plant Endoplasmic Reticulum

  2018cites this paper
• Replication-dependent size reduction precedes differentiation in Chlamydia trachomatis

  2018cites this paper
• THE STUDY OF SNAP29 IN MITOSIS AND IN CEDNIK PATHOGENESIS.

  2018cites this paper
• The Dynamic Nature of the Nuclear Envelope.

  2018cites this paper
• The microenvironment of proliferative diabetic retinopathy supports lymphatic neovascularization

  2018cites this paper
• Dynamic nanoscale morphology of the ER surveyed by STED microscopy

  2018influential citation