In migrating fibroblasts, recycling receptors are concentrated in narrow tubules in the pericentriolar area, and then routed to the plasma membrane of the leading lamella

By following the intracellular processing of recycling transferrin receptors and the selective sorting of a-2 macroglobulin in chick embryo fibroblasts, we have shown that the concentration of 60 nm diam tubules which surrounds the centrioles represents a distal compartment on the recycling pathway. In migrating cells transferrin receptor tracers can be loaded into this compartment and then chased to the cell surface. When they emerge the recycling transferrin receptors are distributed over the surface of the leading lamella.

• Publication year

  1994

• Venue

  Journal of Cell Biology

• Publication date

  1994-06-02

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.125.6.1265PMID 7515888PMCID 2290921
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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  1991influential reference
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  1990cited by this paper
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  1990cited by this paper
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  1982cited by this paper
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  1979cited by this paper
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  1976cited by this paper
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  1976cited by this paper
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  1966influential reference

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