Cells Capable of Bone Production Engraft from Whole Bone Marrow Transplants in Nonablated Mice

Allogeneic and autologous marrow transplants are routinely used to correct a wide variety of diseases. In addition, autologous marrow transplants potentially provide opportune means of delivering genes in transfected, engrafting stem cells. However, relatively little is known about the mechanisms of engraftment in transplant recipients, especially in the nonablated setting and with regard to cells not of hemopoietic origin. In particular, this includes stromal cells and progenitors of the osteoblastic lineage. We have demonstrated for the first time that a whole bone marrow transplant contains cells that engraft and become competent osteoblasts capable of producing bone matrix. This was done at the individual cell level in situ, with significant numbers of donor cells being detected by fluorescence in situ hybridization in whole femoral sections. Engrafted cells were functionally active as osteoblasts producing bone before being encapsulated within the bone lacunae and terminally differentiating into osteocytes. Transplanted cells were also detected as flattened bone lining cells on the periosteal bone surface.

• Publication year

  1999

• Venue

  Journal of Experimental Medicine

• Publication date

  1999-02-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1084/JEM.189.4.729PMID 9989988PMCID 2192923
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Transplanted cells are also found as flattened bone lining cells on the periosteal bone surface.

  Confidence 0.94

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• Engrafted donor cells remain functionally active as osteoblasts, produce bone before being enclosed in bone lacunae, and terminally differentiate into osteocytes.

  Confidence 0.96

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• Whole bone marrow transplants contain donor cells that engraft in nonablated mice and differentiate into competent osteoblasts capable of producing bone matrix.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review

• bone lining cells

  cell type

  Flattened cells that cover quiescent bone surfaces, including the periosteal surface.

  Aliases: lining cells

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• bone matrix

  material

  The mineralized extracellular material produced by osteoblasts during bone formation.

  Aliases: bone

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• fluorescence in situ hybridization

  measurement technique

  A cell- and tissue-level detection method used to identify donor-derived cells in bone sections.

  Aliases: FISH

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• nonablated mice

  animal model

  Recipient mice that were not conditioned by ablation before transplantation.

  Aliases: nonablated setting, nonablated recipients

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• osteoblasts

  cell type

  Bone-forming cells that synthesize and secrete bone matrix.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• osteocytes

  cell type

  Mature bone cells that arise when osteoblasts become embedded within the bone matrix.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review
• whole bone marrow transplant

  transplant procedure

  A transplant of unfractionated marrow cells used here as the donor cell source.

  Aliases: whole marrow transplant, bone marrow transplant

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAK (4715169a40) review

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