A specificity enhancer for polymerase chain reaction.

No abstract is available for this paper.

• Publication year

  1990

• Venue

  Nucleic Acids Research

• Publication date

  1990-08-25

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1093/NAR/18.16.4953PMID 2395670PMCID PMC332015
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• PCR-based detection of a polymorphic BamHI site in intron 1 of the human retinoblastoma (RB) gene.

  1990cited by this paper
• Improvement of PCR amplified DNA sequencing with the aid of detergents.

  1990cited by this paper
• An improved method for directly sequencing PCR amplified material using dimethyl sulphoxide.

  1989cited by this paper
• The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones.

  1988cited by this paper
• Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction.

  1987cited by this paper
• Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

  1985cited by this paper

• Chemical Stability of mRNA/cDNA Complexes: Defining the Limits of mRNA display.

  2024cites this paper
• Techniques, procedures, and applications in host genetic analysis.

  2024cites this paper
• Development of subfamily-based consensus PCR assays for the detection of human and animal herpesviruses

  2023cites this paper
• PCR enhancers: Types, mechanisms, and applications in long-range PCR.

  2022cites this paper
• Insight into the nanomaterials enhancement mechanism of nucleic acid amplification reactions

  2021cites this paper
• A review of reaction enhancement strategies for isothermal nucleic acid amplification reactions

  2021cites this paper
• Polymerase Chain Reaction.

  2019cites this paper
• Optimization of an in-house PCR method for the detection of HLA-B*27 alleles.

  2018cites this paper
• Suppression of Wild-Type Amplification by Selectivity Enhancing Agents in PCR Assays that Utilize SuperSelective Primers for the Detection of Rare Somatic Mutations.

  2018cites this paper
• LOH at 6 q and 10 q in fractionated circulating DNA of ovarian cancer patients is predictive for tumor cell spread and overall survival

  2018cites this paper
• Rapid Microarray-Based Detection of Rifampin, Isoniazid, and Fluoroquinolone Resistance in Mycobacterium tuberculosis by Use of a Single Cartridge

  2017cites this paper
• Evaluation and improvement of LAMP assays for detection of Escherichia coli serogroups O26, O45, O103, O111, O121, O145, and O157

  2017cites this paper
• Comprehensive evaluation of molecular enhancers of the isothermal exponential amplification reaction

  2016cites this paper
• Two Methods for Increased Specificity and Sensitivity in Loop-Mediated Isothermal Amplification

  2015cites this paper
• Diversity of Baculoviruses isolated from cutworms ( Agrotis spp.)

  2015cites this paper
• Detection and quantitation of Agrotis baculoviruses in mixed infections.

  2014cites this paper
• An historical perspective on “The world-wide distribution of allele frequencies at the human dopamine D4 receptor locus”

  2014cites this paper
• Identifizierung und Charakterisierung von neuen Kandidatengenen bei hereditären Skelettmuskelerkrankungen mit kardialer Beteiligung

  2014cites this paper
• Isolation and characterization of 16 highly polymorphic tetranucleotide microsatellite DNA markers in Paa spinosa

  2014cites this paper
• A method for the genetically encoded incorporation of FRET pairs into proteins

  2014cites this paper
• Surface-initiated, reversible polymerization from surface-tethered oligonucleotides by enzymatic processes.

  2013cites this paper
• Optimization of PCR Conditions for Amplification of GC‐Rich EGFR Promoter Sequence

  2013cites this paper
• Single universal primer multiplex ligation-dependent probe amplification with sequencing gel electrophoresis analysis.

  2013cites this paper
• Augmenting PCR: Effects of Additives in the Polymerase Chain Reaction

  2013cites this paper
• An Overview of Nanoparticle‐Assisted Polymerase Chain Reaction Technology

  2013cites this paper
• syphilis by DNA amplification. Detection of Treponema pallidum in early

  2013cites this paper
• RIBOSOMAL DNA FINGERPRINTING OF DIATOM, GENUS PSEUDO-NITZSCHIA YEAP HAN SOON THIS DISSERTATION IS PRESENTED TO FULFILL THE PARTIAL REQUIREMENT TO OBTAIN A BACHELOR OF SCIENCE DEGREE WITH HONOURS PROGRAMME BIOTECHNOLOGY SCHOOL OF SCIENCE AND TECHNOLOGY UNIVERSITI MALAYSIA SABAH

  2013cites this paper
• An Environmental Survey of Forensically Significant Microorganisms Located at the Canberra Airport

  2012cites this paper
• Loss of Heterozygosity at Tumor Suppressor Genes Detectable on Fractionated Circulating Cell-Free Tumor DNA as Indicator of Breast Cancer Progression

  2012cites this paper
• LOH at 6q and 10q in fractionated circulating DNA of ovarian cancer patients is predictive for tumor cell spread and overall survival

  2012cites this paper
• DIMETHYL SULFOXIDE IMPROVES SENSITIVITY AND SPECIFICITY OF RT-PCR AND QRT-PCR AMPLIFICATION OF LOW-EXPRESSED TRANSGENES

  2012cites this paper
• Imaging , Diagnosis , Prognosis Loss of Heterozygosity at Tumor Suppressor Genes Detectable on FractionatedCirculating Cell-Free TumorDNA as Indicator of Breast Cancer Progression

  2012cites this paper
• Prognosis Loss of Heterozygosity at Tumor Suppressor Genes Detectable on FractionatedCirculating Cell-Free TumorDNA as Indicator of Breast Cancer Progression

  2012cites this paper
• Characterization and in vitro expression of non-structural 1 protein of canine parvovirus (CPV-2) in mammalian cell line.

  2011cites this paper
• Ambigols A-C and tjipanazole D: bioinformatic analysis of their putative biosynthetic gene clusters

  2011cites this paper
• Clinical Relevance of Circulating Nucleic Acids in Blood of Prostate Cancer Patients

  2011cites this paper
• Table of Contents Polymerase Pedigree 4 Polymerase Selection Guide 5 Convenience versus Flexibility 6 Taq Polymerase 7 PCR Enhancers and High Yield Buffer system 23 Hot Start Polymerase 12 Proofreading Polymerases

  2011cites this paper
• Eignung unterschiedlicher Primer und DNA-Extraktionsmethoden zum PCR-Nachweisvon Vertretern des Mycobacterium avium-intracellulare-Komplexes (MAIC)

  2011cites this paper
• Optimierung der DNA-Analyse für degradierte biologische Spuren

  2010cites this paper
• Entwicklung eines retroviralen shRNA-basierten Vektorsystems zur Untersuchung BCR-ABL-abhängiger Signalkaskaden in vitro und in vivo

  2010cites this paper
• Improving Protein Solubility via Directed Evolution

  2009cites this paper
• Tetraalkylammonium derivatives as real-time PCR enhancers and stabilizers of the qPCR mixtures containing SYBR Green I

  2008cites this paper
• Identification of loss of heterozygosity on circulating free DNA in peripheral blood of prostate cancer patients: potential and technical improvements.

  2008cites this paper
• New Buffers to Improve the Quantitative Real-Time Polymerase Chain Reaction

  2007cites this paper
• PCR-based detection is unable to consistently distinguish HIV 1LTR circles.

  2006cites this paper
• In Search of Telomeric Fusions in Telomere Mutants of Tetrahymena thermophila

  2006cites this paper
• Approche combinatoire de la production et de la purification de mutants de la streptavidine en vue de générer des métalloenzymes artificielles

  2005cites this paper
• Artificial metalloenzymes: (strept)avidin as host for enantioselective hydrogenation by achiral biotinylated rhodium-diphosphine complexes.

  2004cites this paper
• Developmental expression of N‐methyl‐D‐aspartate glutamate receptor 1 splice variants in the chick retina

  2003cites this paper
• Improved specificity of Y-STR typing in DNA mixture samples

  2003cites this paper
• PCR OPTIMIZATION: IMPROVING OF HUMAN CYTOMEGALOVIRUS (HCMV) PCRTO ACHIEVE A HIGHLY SENSITIVE DETECTION METHOD†

  2003cites this paper
• Experimental versus In Silico Fluorescent Amplified Fragment Length Polymorphism Analysis of Mycobacterium tuberculosis: Improved Typing with an Extended Fragment Range

  2002cites this paper
• GC-Rich Template Amplification by Inverse PCR

  2002cites this paper
• Optimal conditions to use Pfu exo(-) DNA polymerase for highly efficient ligation-mediated polymerase chain reaction protocols.

  2001cites this paper
• An optimized PCR-based procedure for production of 13C/15N-labeled DNA.

  2001cites this paper
• Yarrowia lipolytica SRP receptor α‐subunit

  2000influential citation
• Yarrowia lipolytica SRP receptor alpha-subunit.

  2000cites this paper
• New specificity and yield enhancer of polymerase chain reactions

  2000cites this paper
• Alkaline Proteinase Inhibitor of Pseudomonas aeruginosa

  2000cites this paper
• Current awareness on yeast

  2000cites this paper
• Functional expression and characterisation of a new human phosphatidylinositol 4-kinase PI4K230.

  1999cites this paper
• A second-generation genetic linkage map of the domestic dog, Canis familiaris.

  1999influential citation
• Identification of 5-methylcytosine in complex genomes.

  1999cites this paper
• Mikrosatellitenalterationen in der Serum-DNA bei Patienten mit Bronchialkarzinom

  1999cites this paper
• Co-expression and functional assay of human Rb and E2F1 proteins in yeast

  1999cites this paper
• Molecular analysis of animal and plant cells is facilitated by their attachment to collodion (cellulose nitrate) films.

  1998cites this paper
• Elimination of False Positive Signals for von Willebrand Factor mRNA Detected by Reverse Transcription/PCR Methods Required an Oilgonucleotide Probe Distinct from the PCR Primer Sequences: An Example of a Common Avoidable Pitfall of PCR Technique

  1998cites this paper
• Detection of microorganisms in vessel wall specimens of the abdominal aorta: development of a PCR assay in the absence of a gold standard.

  1998cites this paper
• Cloning heterologous genes: problems and approaches.

  1997cites this paper
• Optimizing multiplex and LA-PCR with betaine.

  1997cites this paper
• Enhancement of microsatellite amplification using tetramethylammonium chloride and formamide (TMACIDE)

  1997cites this paper
• Generation and genetic characterization of immortal human prostate epithelial cell lines derived from primary cancer specimens.

  1997cites this paper
• Cloning gene family members using PCR with degenerate oligonucleotide primers.

  1997cites this paper
• Cloning gene family members using the polymerase chain reaction with degenerate oligonucleotide primers.

  1997cites this paper
• Approaches to DNA mutagenesis: an overview.

  1997cites this paper
• Uniform amplification of a mixture of deoxyribonucleic acids with varying GC content.

  1996cites this paper
• Comparative study of three methods for genotyping hepatitis C virus strains in samples from Spanish patients

  1996cites this paper
• Identification of a 200 kDa polypeptide as type 3 phosphatidylinositol 4-kinase from bovine brain by partial protein and cDNA sequencing.

  1996cites this paper
• 6 – DNA Polymerases

  1996cites this paper
• Polymerase chain Reaction in molecular biotechnology; appropriate technology for developing countries

  1996cites this paper
• Analysis of 99 microdissected prostate carcinomas reveals a high frequency of allelic loss on chromosome 8p12-21.

  1996cites this paper
• The world-wide distribution of allele frequencies at the human dopamine D4 receptor locus

  1996cites this paper
• Strategy for controlling preferential amplification and avoiding false negatives in PCR typing.

  1996cites this paper
• Molecular Methods for the Detection and Identification of Viral Pathogens

  1995cites this paper
• The potential of microsatellites for hybridization‐ and polymerase chain reaction‐based DNA fingerprinting of chickpea (Cicer arietinum L.) and related species

  1995cites this paper
• Genomic fingerprinting by microsatellite-primed PCR: a critical evaluation.

  1995cites this paper
• A Competitive Polymerase Chain Reaction Assay for Reliable Identification of Bordetella Pertussis in Nasopharyngeal Swabs

  1995cites this paper
• Denaturants or cosolvents improve the specificity of PCR amplification of a G + C-rich DNA using genetically engineered DNA polymerases.

  1994cites this paper
• Specific detection of pathogenic Yersinia enterocolitica by two-step PCR using hot-start and DMSO.

  1994cites this paper
• Mycoplasma gallisepticum infection in drug-treated chickens: comparison of diagnosis methods including polymerase chain reaction.

  1994cites this paper
• Identification of Bordetella Pertussis in Nasopharyngeal Swabs Using the Polymerase Chain Reaction: Evaluation of Detection Methods

  1994cites this paper
• Improvement of GC-rich template amplification by inverse PCR.

  1994cites this paper
• Leukocyte esterase urine strips for the screening of men with urethritis--use in developing countries.

  1994cites this paper
• Interlaboratory agreement study of a double set of PCR plasmid primers for detection of Chlamydia trachomatis in a variety of genitourinary specimens

  1994cites this paper
• The CRY1 gene in Chlamydomonas reinhardtii: structure and use as a dominant selectable marker for nuclear transformation

  1994cites this paper
• Comparison of antigenic and pathogenic properties of Mycoplasma iowae strains and development of a PCR-based detection assay.

  1994cites this paper
• Oligonucleotides as Short as 7-Mers Can Be Used for PCR Amplification

  1994cites this paper
• The DNA binding domain of retinoic acid receptor beta is required for ligand-dependent suppression of proliferation. Application of general purpose mammalian coexpression vectors.

  1994cites this paper
• Betaine can eliminate the base pair composition dependence of DNA melting.

  1993cites this paper
• Recombinant Baculovirus Vectors Expressing Glutathione–S–Transferase Fusion Proteins

  1993cites this paper