Betaine can eliminate the base pair composition dependence of DNA melting.

No abstract is available for this paper.

• Publication year

  1993

• Venue

  Biochemistry

• Publication date

  1993-01-12

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/BI00052A019PMID 8418834
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Enzyme activities in concentrated solutions of glycinebetaine and other solutes

  2004cited by this paper
• Origins of the osmoprotective properties of betaine and proline in Escherichia coli K-12

  1992cited by this paper
• Increased thermal stability of proteins in the presence of naturally occurring osmolytes.

  1992cited by this paper
• Detection of single base mismatches of thymine and cytosine residues by potassium permanganate and hydroxylamine in the presence of tetralkylammonium salts.

  1990cited by this paper
• A specificity enhancer for polymerase chain reaction.

  1990cited by this paper
• The TEACL method of DNA-DNA hybridization: Technical considerations

  1990cited by this paper
• The thermal stability of oligonucleotide duplexes is sequence independent in tetraalkylammonium salt solutions: application to identifying recombinant DNA clones.

  1988cited by this paper
• Differential effect of tetramethylammonium chloride and sodium chloride on duplex melting temperatures of deoxyoligonucleotides: resolution of a salt effect into specific and non-specific components

  1988cited by this paper
• Hybridization of genomic DNA to oligonucleotide probes in the presence of tetramethylammonium chloride.

  1987cited by this paper
• Predicting DNA duplex stability from the base sequence.

  1986cited by this paper
• Improved free-energy parameters for predictions of RNA duplex stability.

  1986cited by this paper
• Glycine betaine transport in Escherichia coli: osmotic modulation

  1985cited by this paper
• The stabilization of proteins by osmolytes.

  1985cited by this paper
• Base composition-independent hybridization in tetramethylammonium chloride: a method for oligonucleotide screening of highly complex gene libraries.

  1985cited by this paper
• Distribution of counterions around a cylindrical polyelectrolyte and manning's condensation theory

  1984cited by this paper
• Preferential interactions of proteins with solvent components in aqueous amino acid solutions.

  1983cited by this paper
• Assignment of resonances in the phosphorus-31 nuclear magnetic resonance spectrum of poly[d(A-T)] from phosphorothioate substitution.

  1983cited by this paper
• Living with water stress: evolution of osmolyte systems.

  1982cited by this paper
• Right-handed alternating DNA conformation: poly(dA-dT) adopts the same dinucleotide repeat with cesium, tetraalkylammonium, and 3 alpha, 5 beta, 17 beta-dipyrrolidinium steroid dimethiodide cations in aqueous solution.

  1981cited by this paper
• Characterization of alternating deoxyribonucleic acid conformations in solution by phosphorus-31 nuclear magnetic resonance spectroscopy.

  1981cited by this paper
• A hypothesis on a specific sequence-dependent conformation of DNA and its relation to the binding of the lac-repressor protein.

  1979cited by this paper
• An alternating conformation characterizes the phosphodiester backbone of poly(dA-dT) in solution.

  1979cited by this paper
• Thermodynamic analysis of ion effects on the binding and conformational equilibria of proteins and nucleic acids: the roles of ion association or release, screening, and ion effects on water activity

  1978cited by this paper
• The molecular theory of polyelectrolyte solutions with applications to the electrostatic properties of polynucleotides

  1978cited by this paper
• Supercoiling in closed circular DNA: dependence upon ion type and concentration.

  1978cited by this paper
• Theoretical calculations of the helix–coil transition of DNA in the presence of large, cooperatively binding ligands

  1976cited by this paper
• Ion effects on ligand-nucleic acid interactions.

  1976cited by this paper
• Theoretical aspects of DNA-protein interactions: co-operative and non-co-operative binding of large ligands to a one-dimensional homogeneous lattice.

  1974cited by this paper
• Alteration of the relative stability of dA-dT and dG-dC base pairs in DNA.

  1973cited by this paper
• Different conformations of double‐stranded nucleic acid in solution as revealed by circular dichroism

  1973cited by this paper
• The binding of small cations to deoxyribonucleic acid. Nucleotide specificity.

  1969cited by this paper
• On the conformational stability of globular proteins. The effects of various electrolytes and nonelectrolytes on the thermal ribonuclease transition.

  1965cited by this paper
• Dependence of the melting temperature of DNA on salt concentration

  1965cited by this paper
• SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.

  1965cited by this paper
• SOME OBSERVATIONS ON THE HYPOCHROMISM OF DNA.

  1964cited by this paper
• Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature.

  1962cited by this paper
• Helix-random coil transitions in synthetic DNAs of alternating sequence.

  1962cited by this paper

• DNA polymerase switch-mediated CRISPR/Cas system for photoelectrochemical detection of apurinic/apyrimidinic endonuclease 1

  2026cites this paper
• Label-free detection of microRNA by polymerization and isomerization cyclic amplification coupled with G/Hemin DNAzyme

  2025cites this paper
• Ternary Choline Chloride-Based Deep Eutectic Solvents: A Review

  2025cites this paper
• Programmable DNA Dendrimer from a Single Symmetric Y‐Shaped Structural Unit for Targeted Tumor Therapy with One Hundred Inhibition Efficiency

  2025cites this paper
• Isothermal assembly of DNA nanostructures.

  2025cites this paper
• Effect of osmolytes-TMAO and serine on the binding and stability of 5-fluorouracil with calf thymus DNA (CT-DNA): biophysical insight.

  2025cites this paper
• High-sensitivity qPCR detection method based on silver flower-like LSPR-active material

  2025cites this paper
• Development of a portable paper-based biosensor for the identification of genetically modified corn (Zea mays) and soybean (Glycine max).

  2025cites this paper
• Development of a real-time loop-mediated isothermal amplification (real-time LAMP) assay for the onsite detection of Vibrio parahaemolyticus causing acute hepatopancreatic necrosis disease (AHPND) in shrimp.

  2025cites this paper
• The improvement of PCR amplification pattern by PCR enhancers and formulation novel PCR enhancer

  2024influential citation
• Alteration of reactive oxygen species master transcription factor Nrf2 in keratinocytes exposed to monoclonal pathogenic antibody AK23 against desmoglein-3 in pemphigus vulgaris

  2024cites this paper
• Sweet enhancers of polymerase chain reaction

  2024cites this paper
• DNA Self-Assembly Optimization by Betaine and Its Analogs.

  2024cites this paper
• Refining the Schistosoma haematobium recombinase polymerase amplification (Sh-RPA) assay: moving towards point-of-care use in endemic settings

  2024cites this paper
• Bacteria and Yeast Colony PCR.

  2023cites this paper
• A SMART method for isolating monoclonal antibodies from individual rhesus macaque memory B cells.

  2023cites this paper
• PCR inhibitors and facilitators - Their role in forensic DNA analysis.

  2023cites this paper
• DNA nanotechnology in ionic liquids and deep eutectic solvents

  2023cites this paper
• A SMART method for efficiently isolating monoclonal antibodies from individual rhesus macaque memory B cells

  2023cites this paper
• Dry LAMP: A point of care diagnostics for diagnosis of bovine tropical theileriosis.

  2023cites this paper
• Current and Future Advances in the Detection and Surveillance of Biosecurity-Relevant Equine Bacterial Diseases Using Loop-Mediated Isothermal Amplification (LAMP)

  2023cites this paper
• Relationship between prokaryotic GC contents and environmental salinity

  2023cites this paper
• Relationship between prokaryotic GC content and environmental salinity

  2023cites this paper
• PCR enhancers: Types, mechanisms, and applications in long-range PCR.

  2022cites this paper
• Target recycling-triggered polymerization/isomerization amplification cascades for sensitive microRNA assay

  2022cites this paper
• Effect of storage temperature and duration on direct PCR amplification of various feather types and DBS matrices.

  2022cites this paper
• procedures to amplify GC-rich DNA sequences

  2022cites this paper
• Highly Sensitive EGFRvIII Detection in Circulating Extracellular Vesicle RNA of Glioma Patients

  2022cites this paper
• An EPR and VTVH MCD spectroscopic investigation of the nitrogenase assembly protein NifB

  2021cites this paper
• Polymerization and isomerization cyclic amplification for nucleic acid detection with attomolar sensitivity

  2021cites this paper
• Alkaline lysis‐loop mediated isothermal amplification assay for rapid and on‐site authentication of buffalo ( Bubalus bubalis ) meat

  2021cites this paper
• Low stutter ratio by SuperFi polymerase

  2021cites this paper
• Development of a Loop-Mediated Isothermal Amplification Method for the Rapid Detection of Phytopythium vexans

  2021cites this paper
• A review of reaction enhancement strategies for isothermal nucleic acid amplification reactions

  2021cites this paper
• The glycine betaine role in neurodegenerative, cardiovascular, hepatic, and renal diseases: Insights into disease and dysfunction networks.

  2021cites this paper
• PCR procedures to amplify GC-rich DNA sequences of Mycobacterium bovis

  2020cites this paper
• Aqueous solution of betaine: Hydration and aggregation

  2020cites this paper
• pH-Responsive Reversible DNA Self-assembly Mediated by Zwitterion

  2020cites this paper
• Fast, Precise, and Reliable Multiplex Detection of Potato Viruses by Loop-Mediated Isothermal Amplification

  2020cites this paper
• On-Site Detection of Tissues of Buffalo Origin by Loop-Mediated Isothermal Amplification (LAMP) Assay Targeting Mitochondrial Gene Sequences

  2020cites this paper
• Development of a Linker Mediated Immunoassay Using Chemically Transitioned Nanosensors.

  2020cites this paper
• Solid-Phase Microextraction Enables Isolation of BRAF V600E Circulating Tumor DNA from Human Plasma for Detection with a Molecular Beacon Loop-Mediated Isothermal Amplification Assay.

  2020cites this paper
• Development of reverse transcription loop-mediated isothermal amplification (RT-LAMP) and reverse transcription recombinase polymerase amplification (RT-RPA) assays for the detection of two novel viruses infecting ginger.

  2020cites this paper
• pH-Responsive Reversible DNA Self-assembly Mediated by Zwitterion

  2020cites this paper
• Betaine-assisted recombinase polymerase assay with enhanced specificity.

  2019cites this paper
• Progress in loop-mediated isothermal amplification assay for detection of Schistosoma mansoni DNA: towards a ready-to-use test

  2019cites this paper
• Polymerase Chain Reaction (PCR) Amplification of GC-Rich Templates.

  2019cites this paper
• Dual-Priming Isothermal Amplification (DAMP) for Highly Sensitive and Specific Molecular Detection with Ultralow Nonspecific Signals.

  2019cites this paper
• Electron Paramagnetic Resonance and Magnetic Circular Dichroism Spectra of the Nitrogenase M Cluster Precursor Suggest Sulfur Migration upon Oxidation: A Proposal for Substrate and Inhibitor Binding

  2019cites this paper
• Highly specific enrichment of rare nucleic acid fractions using Thermus thermophilus argonaute with applications in cancer diagnostics

  2019cites this paper
• Rapid assessment of viral water quality using a novel recombinase polymerase amplification test for human adenovirus

  2019cites this paper
• Enzymatic Synthesis of Backbone‐Modified Oligonucleotides Using T4 DNA Ligase

  2019cites this paper
• Bridged Oligonucleotides with Smoothed Hybridization Properties as a Tool for Analysis of Nucleotide Sequences

  2019cites this paper
• A microfluidic chip capable of generating and trapping emulsion droplets for digital loop-mediated isothermal amplification analysis.

  2018cites this paper
• A Simple Whole-Blood Polymerase Chain Reaction without DNA Extraction for Thalassemia Diagnosis

  2018cites this paper
• Oligonucleotide microarray methodology for taxonomic and functional monitoring of microbial community composition.

  2018cites this paper
• The detection of ACLSV and ASPV in pear plants by RT-LAMP assays.

  2018cites this paper
• XNA ligation using T4 DNA ligase in crowding conditions.

  2018cites this paper
• A novel PCR method directly quantifies sequence features that block primer extension

  2018cites this paper
• Template-Independent Enzymatic Oligonucleotide Synthesis (TiEOS): Its History, Prospects, and Challenges.

  2018cites this paper
• Combination of Zn2+ and betaine can eliminate the effect of DNA fragments with different GC content on gene chip.

  2018cites this paper
• Potent and Selective Inhibitors of 8-Oxoguanine DNA Glycosylase.

  2018cites this paper
• Effect of osmolytes of different type on DNA behavior in aqueous solution. Experimental and theoretical studies

  2018cites this paper
• Improved molecular karyotyping in glioblastoma.

  2018cites this paper
• A vertical flow paper-microarray assay with isothermal DNA amplification for detection of Neisseria meningitidis.

  2018cites this paper
• Simplified Real-Time Multiplex Detection of Loop-Mediated Isothermal Amplification Using Novel Mediator Displacement Probes with Universal Reporters.

  2018cites this paper
• Attomolar detection of extracellular microRNAs released from living prostate cancer cells by a plasmonic nanowire interstice sensor.

  2017cites this paper
• Colony PCR.

  2017cites this paper
• A universal method for direct PCR amplification of plant tissues

  2017cites this paper
• SYSTEMAND METHOD FOR SORTING CELLS

  2017cites this paper
• l-Proline and RNA Duplex m-Value Temperature Dependence.

  2017cites this paper
• Ligation-mediated PCR with a back-to-back adapter reduces amplification bias resulting from variations in GC content.

  2017cites this paper
• Coherent Experimental and Simulation Approach To Explore the Underlying Mechanism of Denaturation of Stem Bromelain in Osmolytes.

  2017cites this paper
• Accelerated isothermal nucleic acid amplification in betaine-free reaction.

  2017cites this paper
• Sequence-specific validation of LAMP amplicons in real-time optomagnetic detection of Dengue serotype 2 synthetic DNA.

  2017cites this paper
• Modified allele-specific PCR improves HER2 Ile655Val detection by reducing genotyping errors

  2017cites this paper
• Single Molecule Approaches to Mapping DNA Replication Origins

  2017cites this paper
• Recent advances in assays for the fragile X-related disorders

  2017cites this paper
• Chapter 8 – RT-PCR: A Science and an Art Form

  2017cites this paper
• ThermoAlign: a genome-aware primer design tool for tiled amplicon resequencing

  2017cites this paper
• Loop-mediated isothermal amplification ( lamp ) : furtherance of assorted detection systems towards point-of-care settings

  2017cites this paper
• Comprehensive evaluation of molecular enhancers of the isothermal exponential amplification reaction

  2016cites this paper
• Improved PCR Amplification of Broad Spectrum GC DNA Templates

  2016cites this paper
• Basis of Protein Stabilization by K Glutamate: Unfavorable Interactions with Carbon, Oxygen Groups.

  2016cites this paper
• Water modulates the ultraslow dynamics of hydrated ionic liquids near CG rich DNA: consequences for DNA stability.

  2016cites this paper
• Betaine chemistry, roles, and potential use in liver disease.

  2016cites this paper
• Effects of trimethylamine N-oxide and urea on DNA duplex and G-quadruplex

  2016cites this paper
• ISSR MARKERS FOR DETERMINATION OF GENETIC DIVERSITY IN SPILANTHES SPECIES - MEDICINAL HERB FROM PENINSULAR INDIA

  2016cites this paper
• PCR amplification of GC-rich DNA regions using the nucleotide analog N4-methyl-2'-deoxycytidine 5'-triphosphate.

  2016cites this paper
• Effects of Salt on the Stability of a G-Quadruplex from the Human c-MYC Promoter.

  2015cites this paper
• Development of amplification-based technologies for enrichment of nucleic acids with difficult sequences or low-abundance point mutations

  2015cites this paper
• Isothermal DNA origami folding: avoiding denaturing conditions for one-pot, hybrid-component annealing.

  2015cites this paper
• Whole Blood PCR Amplification with Pfu DNA Polymerase and Its Application in Single-Nucleotide Polymorphism Analysis

  2015cites this paper
• Functional analysis of type III secretion system and transcription activator-like effectors of Xanthomonas translucens, the causal agent of bacterial leaf streak on cereals

  2015cites this paper
• Effect of osmolytes on the binding of EGR1 transcription factor to DNA

  2015cites this paper
• Functional and expression analyses of two kinds of betaine aldehyde dehydrogenases in a glycinebetaine-hyperaccumulating graminaceous halophyte, Leymus chinensis

  2015cites this paper
• Its preferential interactions with biopolymers account for diverse observed effects of trehalose.

  2015cites this paper
• Small Molecule Osmolytes Can Modulate Proteostasis

  2015cites this paper
• Loop-Mediated Isothermal Amplification Procedure (LAMP) for Detection of the Potato Zebra Chip Pathogen "Candidatus Liberibacter solanacearum".

  2015cites this paper
• Highly thermostable RadA protein from the archaeon Pyrococcus woesei enhances specificity of simplex and multiplex PCR assays

  2015cites this paper