CDC42 switches IRSp53 from inhibition of actin growth to elongation by clustering of VASP

No abstract is available for this paper.

• Publication year

  2013

• Venue

  EMBO Journal

• Publication date

  2013-09-27

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/emboj.2013.208PMID 24076653PMCID PMC3801440
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Faculty Opinions recommendation of Filopodia initiation: focus on the Arp2/3 complex and formins.

  2015cited by this paper
• Arp2/3 is critical for lamellipodia and response to extracellular matrix cues but is dispensable for chemotaxis.

  2012cited by this paper
• The Eps8/IRSp53/VASP Network Differentially Controls Actin Capping and Bundling in Filopodia Formation

  2011cited by this paper
• Filopodia initiation

  2011cited by this paper
• Individual Actin Filaments in a Microfluidic Flow Reveal the Mechanism of ATP Hydrolysis and Give Insight Into the Properties of Profilin

  2011cited by this paper
• mDia1 and WAVE2 Proteins Interact Directly with IRSp53 in Filopodia and Are Involved in Filopodium Formation

  2011cited by this paper
• I-BAR domain proteins: linking actin and plasma membrane dynamics.

  2011influential reference
• Molecular mechanism of Ena/VASP-mediated actin-filament elongation

  2011cited by this paper
• Plithotaxis and emergent dynamics in collective cellular migration.

  2011cited by this paper
• Visualizing branched actin filaments in lamellipodia by electron tomography

  2011cited by this paper
• I-BAR domains, IRSp53 and filopodium formation.

  2010cited by this paper
• A Steering Model of Endothelial Sheet Migration Recapitulates Monolayer Integrity and Directed Collective Migration

  2010cited by this paper
• VASP is a processive actin polymerase that requires monomeric actin for barbed end association

  2010influential reference
• Protease-dependent versus -independent cancer cell invasion programs: three-dimensional amoeboid movement revisited

  2009cited by this paper
• The Role of Stromal Stem Cells in Tissue Regeneration and Wound Repair

  2009cited by this paper
• IRSp53 links the enterohemorrhagic E. coli effectors Tir and EspFU for actin pedestal formation.

  2009cited by this paper
• Ena/VASP: towards resolving a pointed controversy at the barbed end

  2009cited by this paper
• The Insulin Receptor Substrate of 53 kDa (IRSp53) Limits Hippocampal Synaptic Plasticity*

  2009cited by this paper
• Eps8 Regulates Axonal Filopodia in Hippocampal Neurons in Response to Brain-Derived Neurotrophic Factor (BDNF)

  2009cited by this paper
• A novel flow cytometric assay to quantify interactions between proteins and membrane lipids This study was supported by the Collaborative Research Center 638 of the German Research Foundation. Published, JLR Papers in Press, January 14, 2009.

  2009cited by this paper
• F- and G-Actin Concentrations in Lamellipodia of Moving Cells

  2009cited by this paper
• Coordination of Membrane and Actin Cytoskeleton Dynamics during Filopodia Protrusion

  2009influential reference
• Filopodia: Complex models for simple rods.

  2009cited by this paper
• VASP is a CXCR2-interacting protein that regulates CXCR2-mediated polarization and chemotaxis

  2009cited by this paper
• Ena/VASP Proteins Capture Actin Filament Barbed Ends*

  2008cited by this paper
• IRSp53: crossing the road of membrane and actin dynamics in the formation of membrane protrusions.

  2008cited by this paper
• The Cdc42 Effector IRSp53 Generates Filopodia by Coupling Membrane Protrusion with Actin Dynamics*

  2008cited by this paper
• Traction Dynamics of Filopodia on Compliant Substrates

  2008cited by this paper
• Clustering of VASP actively drives processive, WH2 domain‐mediated actin filament elongation

  2008cited by this paper
• Capping protein increases the rate of actin-based motility by promoting filament nucleation by the Arp2/3 complex.

  2008cited by this paper
• Reorganisation of the dendritic actin network during cancer cell migration and invasion.

  2008cited by this paper
• Membrane targeting of WAVE2 is not sufficient for WAVE2-dependent actin polymerization: a role for IRSp53 in mediating the interaction between Rac and WAVE2

  2008cited by this paper
• Structural basis for the recruitment of profilin–actin complexes during filament elongation by Ena/VASP

  2007cited by this paper
• Enabled plays key roles in embryonic epithelial morphogenesis in Drosophila

  2007cited by this paper
• The beta-thymosin/WH2 fold: multifunctionality and structure.

  2007cited by this paper
• Ena/VASP proteins have an anti-capping independent function in filopodia formation.

  2007influential reference
• Filopodia are required for cortical neurite initiation

  2007cited by this paper
• Novel Roles of Formin mDia2 in Lamellipodia and Filopodia Formation in Motile Cells

  2007cited by this paper
• Ena/VASP Is Required for neuritogenesis in the developing cortex.

  2007cited by this paper
• Role of fascin in filopodial protrusion

  2006cited by this paper
• The bundling activity of vasodilator-stimulated phosphoprotein is required for filopodium formation

  2006cited by this paper
• Regulation of cell shape by Cdc42 is mediated by the synergic actin-bundling activity of the Eps8–IRSp53 complex

  2006influential reference
• Formins and VASPs may co-operate in the formation of filopodia

  2005cited by this paper
• Formins and VASPs may co-operate in the formation of filopodia.

  2005cited by this paper
• The Rho family GTPase Rif induces filopodia through mDia2.

  2005cited by this paper
• Structural basis of filopodia formation induced by the IRSp53/MIM homology domain of human IRSp53

  2005cited by this paper
• Ena/VASP Proteins Enhance Actin Polymerization in the Presence of Barbed End Capping Proteins*[boxs]

  2005cited by this paper
• Mena and Vasodilator-Stimulated Phosphoprotein Are Required for Multiple Actin-Dependent Processes That Shape the Vertebrate Nervous System

  2004cited by this paper
• Lamellipodial versus filopodial mode of the actin nanomachinery: pivotal role of the filament barbed end.

  2004cited by this paper
• Roles of fascin in cell adhesion and motility.

  2004cited by this paper
• Eps8 controls actin-based motility by capping the barbed ends of actin filaments

  2004cited by this paper
• A Novel Actin Bundling/Filopodium-forming Domain Conserved in Insulin Receptor Tyrosine Kinase Substrate p53 and Missing in Metastasis Protein*

  2004cited by this paper
• Lamellipodin, an Ena/VASP ligand, is implicated in the regulation of lamellipodial dynamics.

  2004cited by this paper
• Insertional assembly of actin filament barbed ends in association with formins produces piconewton forces.

  2004cited by this paper
• The myofibroblast in wound healing and fibrocontractive diseases

  2003cited by this paper
• Disruption of the Diaphanous-related formin Drf1 gene encoding mDia1 reveals a role for Drf3 as an effector for Cdc42.

  2003cited by this paper
• A biomimetic motility assay provides insight into the mechanism of actin-based motility

  2003cited by this paper
• How VASP enhances actin-based motility

  2003cited by this paper
• Mechanism of filopodia initiation by reorganization of a dendritic network

  2003influential reference
• ProSAP/Shank postsynaptic density proteins interact with insulin receptor tyrosine kinase substrate IRSp53

  2002cited by this paper
• Antagonism between Ena/VASP proteins and actin filament capping regulates fibroblast motility.

  2002cited by this paper
• Cdc42hs Facilitates Cytoskeletal Reorganization and Neurite Outgrowth by Localizing the 58-Kd Insulin Receptor Substrate to Filamentous Actin

  2001cited by this paper
• Cdc42 induces filopodia by promoting the formation of an IRSp53:Mena complex.

  2001cited by this paper
• IRSp53 is an essential intermediate between Rac and WAVE in the regulation of membrane ruffling

  2000cited by this paper
• VASP dynamics during lamellipodia protrusion

  1999influential reference
• Identification of BAIAP2 (BAI-associated protein 2), a novel human homologue of hamster IRSp53, whose SH3 domain interacts with the cytoplasmic domain of BAI1

  1999cited by this paper
• The EVH2 Domain of the Vasodilator-stimulated Phosphoprotein Mediates Tetramerization, F-actin Binding, and Actin Bundle Formation*

  1999cited by this paper
• Dentatorubral-pallidoluysian atrophy protein interacts through a proline-rich region near polyglutamine with the SH3 domain of an insulin receptor tyrosine kinase substrate.

  1999cited by this paper
• Characterization of the actin binding properties of the vasodilator‐stimulated phosphoprotein VASP

  1999cited by this paper
• Arp2/3 Complex and Actin Depolymerizing Factor/Cofilin in Dendritic Organization and Treadmilling of Actin Filament Array in Lamellipodia

  1999cited by this paper
• The Insulin Receptor Tyrosine Kinase Substrate p58/53 and the Insulin Receptor Are Components of CNS Synapses

  1999cited by this paper
• Mena is required for neurulation and commissure formation.

  1999cited by this paper
• Mena, a relative of VASP and Drosophila Enabled, is implicated in the control of microfilament dynamics.

  1996cited by this paper
• enabled, a dosage-sensitive suppressor of mutations in the Drosophila Abl tyrosine kinase, encodes an Abl substrate with SH3 domain-binding properties.

  1995cited by this paper
• The Ras-related protein Cdc42Hs and bradykinin promote formation of peripheral actin microspikes and filopodia in Swiss 3T3 fibroblasts

  1995cited by this paper
• Identification, purification, and characterization of a zyxin-related protein that binds the focal adhesion and microfilament protein VASP (vasodilator-stimulated phosphoprotein).

  1995cited by this paper
• HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization.

  1987cited by this paper

• Cdc42 is crucial for the early regulation of hepatic stellate cell activation.

  2025cites this paper
• Biophysical and Molecular mechanisms that control active wetting and tissue fluidification in epithelial tissues

  2025cites this paper
• Endophilin–lamellipodin–VASP, key components in fast endophilin–mediated endocytosis, control actin polymerization within liquid-like condensates

  2025cites this paper
• Protrusion‐Derived Extracellular Vesicles (PD‐EVs) and Their Diverse Origins: Key Players in Cellular Communication, Cancer Progression, and T Cell Modulation

  2025cites this paper
• Slik sculpts the plasma membrane into cytonemes to control cell-cell communication

  2025cites this paper
• NHSL3 controls single and collective cell migration through two distinct mechanisms

  2025cites this paper
• Numerical studies of triangulated vesicles with anisotropic membrane inclusions

  2024cites this paper
• STRIPAK controls cell-cell communication by promoting cytoneme biogenesis through the membrane-sculpting function of Slik

  2024cites this paper
• Scar/WAVE drives actin protrusions independently of its VCA domain using proline-rich domains.

  2024cites this paper
• Tunneling Nanotubes: The Cables for Viral Spread and Beyond.

  2024cites this paper
• Actin-membrane linkers: Insights from synthetic reconstituted systems.

  2024cites this paper
• Intercellular adhesion boots collective cell migration through elevated membrane tension

  2024cites this paper
• Probing the protrusions: lamellipodia and filopodia in cancer invasion and beyond

  2024cites this paper
• Reconstitution of the transition from a lamellipodia- to filopodia-like actin network with purified proteins.

  2023cites this paper
• Filopodial protrusion driven by density-dependent Ena–TOCA-1 interactions

  2023cites this paper
• Macrophages modulate stiffness-related foreign body responses through plasma membrane deformation

  2023cites this paper
• Membrane reshaping by protein condensates.

  2023cites this paper
• Unleashed Actin Assembly in Capping Protein-Deficient B16-F1 Cells Enables Identification of Multiple Factors Contributing to Filopodium Formation

  2023cites this paper
• Lissencephaly-associated BAIAP2 variant causes defects in neuronal migration during brain development.

  2023cites this paper
• Tunnelling nanotube formation is driven by Eps8/IRSp53‐dependent linear actin polymerization

  2023influential citation
• Negatively curved cellular membranes promote BAIAP2 signaling hub assembly.

  2023cites this paper
• BIN1 regulates actin-membrane interactions during IRSp53-dependent filopodia formation

  2023influential citation
• Fusion pore dynamics of large secretory vesicles define a distinct mechanism of exocytosis

  2023cites this paper
• Filopodia In Vitro and In Vivo.

  2023cites this paper
• Molecular Relay Stations in Membrane Nanotubes: IRSp53 Involved in Actin-Based Force Generation

  2023influential citation
• A mechanosensing mechanism controls plasma membrane shape homeostasis at the nanoscale

  2023cites this paper
• Actin-based protrusions at a glance.

  2023cites this paper
• Liquid-like VASP condensates drive actin polymerization and dynamic bundling

  2022cites this paper
• Liquid-like assembly of VASP drives actin polymerization and bundling

  2022cites this paper
• SARS-CoV-2 replication in airway epithelia requires motile cilia and microvillar reprogramming

  2022cites this paper
• The Scar/WAVE complex drives normal actin protrusions without the Arp2/3 complex, but proline-rich domains are required

  2022cites this paper
• Arp2/3 inhibition switches Eps8’s network associations to favour longer actin filament formation necessary for tunneling nanotubes

  2022influential citation
• Ena/VASP clustering at microspike tips involves lamellipodin but not I-BAR proteins, and absolutely requires unconventional myosin-X

  2022cites this paper
• Cooperative assembly of filopodia by the formin FMNL2 and I-BAR domain protein IRTKS

  2022cites this paper
• ARHGEF9 regulates melanoma morphogenesis in environments with diverse geometry and elasticity by promoting filopodial-driven adhesion

  2022cites this paper
• Biochemical and mechanical regulation of actin dynamics

  2022cites this paper
• VASP localization to lipid bilayers induces polymerization driven actin bundle formation

  2022influential citation
• BIN1/Amphiphysin 2 and ezrin drive filopodia-like structures in myoblasts

  2022cites this paper
• Activated I-BAR IRSp53 clustering controls the formation of VASP-actin–based membrane protrusions

  2022influential citation
• A mechanosensing mechanism mediated by IRSp53 controls plasma membrane shape homeostasis at the nanoscale

  2021cites this paper
• Cargo-specific recruitment in clathrin- and dynamin-independent endocytosis

  2021cites this paper
• Actin dynamics during tumor cell dissemination.

  2021cites this paper
• Full assembly of HIV-1 particles requires assistance of the membrane curvature factor IRSp53

  2021cites this paper
• Stochastic combinations of actin regulatory proteins are sufficient to drive filopodia formation

  2021influential citation
• Vangl2 promotes the formation of long cytonemes to enable distant Wnt/β-catenin signaling

  2021cites this paper
• Comparing physical mechanisms for membrane curvature-driven sorting of BAR-domain proteins.

  2021cites this paper
• Role of Tunneling Nanotubes in Viral Infection, Neurodegenerative Disease, and Cancer

  2021cites this paper
• Computation, wiring, and plasticity in synaptic clusters.

  2021cites this paper
• Dendrite tapering actuates a self-organizing signaling circuit for stochastic filopodia initiation in neurons

  2021cites this paper
• Dissecting the Inorganic Nanoparticle-Driven Interferences on Adhesome Dynamics

  2021cites this paper
• A teamwork promotion of formin-mediated actin nucleation by Bud6 and Aip5 in Saccharomyces cerevisiae

  2021cites this paper
• Fine intercellular connections in development: TNTs, cytonemes, or intercellular bridges?

  2020cites this paper
• Cargo-specific recruitment in clathrin and dynamin-independent endocytosis

  2020cites this paper
• Leading-edge VASP clusters assemble at sites containing lamellipodin and exhibit size-dependent instability

  2020influential citation
• Initiation and disassembly of filopodia tip complexes containing VASP and lamellipodin

  2020influential citation
• Palmitoylated Proteins in Dendritic Spine Remodeling

  2020cites this paper
• IRSp53 is a novel interactor of SHIP2: A role of the actin binding protein Mena in their cellular localization in breast cancer cells.

  2020cites this paper
• The Ways of Actin: Why Tunneling Nanotubes Are Unique Cell Protrusions.

  2020cites this paper
• Localisation de l'ARNm STAT3 aux protrusions des cellules du carcinome hépatocellulaire associées à un phénotype métastatique

  2020cites this paper
• IRSp53 controls plasma membrane shape and polarized transport at the nascent lumen in epithelial tubules

  2020cites this paper
• Roles of Interactions Between Cells and Extracellular Matrices for Cell Migration and Matrix Remodeling

  2019cites this paper
• Proteomics Analysis Identifies IRSp53 and Fascin as Critical for PRV Egress and Direct Cell–Cell Transmission

  2019cites this paper
• Glycerophospholipids - Emerging players in neuronal dendrite branching and outgrowth.

  2019cites this paper
• ROLE OF IRSP53 IN EPITHELIAL POLARITY ESTABLISHMENT AND LUMEN MORPHOGENESIS

  2019influential citation
• Filopodia and their links with membrane traffic and cell adhesion.

  2019cites this paper
• IRSp53 shapes the plasma membrane and controls polarized transport at the nascent lumen during epithelial morphogenesis

  2019cites this paper
• Filopodia and focal adhesions: An integrated system driving branching morphogenesis in neuronal pathfinding and angiogenesis.

  2019cites this paper
• IRSp53 coordinates AMPK and 14-3-3 signaling to regulate filopodia dynamics and directed cell migration

  2019cites this paper
• PKA negatively modulates the migration enhancing effect of Connexin 43.

  2019cites this paper
• Constrained actin dynamics emerges from variable compositions of actin regulatory protein complexes

  2019cites this paper
• Superresolution microscopy reveals distinct localisation of full length IRSp53 and its I-BAR domain protein within filopodia

  2019cites this paper
• Inception Mechanisms of Tunneling Nanotubes

  2019influential citation
• Receptor-Free Signaling at Curved Cellular Membranes.

  2019cites this paper
• Membrane re-modelling by BAR domain superfamily proteins via molecular and non-molecular factors.

  2018cites this paper
• Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis

  2018cites this paper
• Ezrin enrichment on curved membranes requires a specific conformation or interaction with a curvature-sensitive partner

  2018cites this paper
• Rab11a–Rab8a cascade regulates the formation of tunneling nanotubes through vesicle recycling

  2018cites this paper
• Wnt / PCP controls spreading of Wnt / β-catenin signals by cytonemes in 1 vertebrates 2 3 4

  2018cites this paper
• Theoretical study of vesicle shapes driven by coupling curved proteins and active cytoskeletal forces.

  2018cites this paper
• Label‐Free Quantitative Phosphoproteomics Reveals Regulation of Vasodilator‐Stimulated Phosphoprotein upon Stathmin‐1 Silencing in a Pair of Isogenic Colorectal Cancer Cell Lines

  2018cites this paper
• Guided by curvature: shaping cells by coupling curved membrane proteins and cytoskeletal forces

  2018cites this paper
• Mechanism of IRSp53 inhibition by 14-3-3

  2018cites this paper
• Roles for Ena/VASP proteins in FMNL3-mediated filopodial assembly

  2018influential citation
• THE DUAL ROLE OF YAP IN DRIVING TGFß-MEDIATED ENDMT

  2018cites this paper
• BAR domain proteins—a linkage between cellular membranes, signaling pathways, and the actin cytoskeleton

  2018cites this paper
• Enhanced capture and release of circulating tumor cells using hollow glass microspheres with a nanostructured surface.

  2018cites this paper
• Ena/VASP processive elongation is modulated by avidity on actin filaments bundled by the filopodia cross-linker fascin

  2018cites this paper
• Publisher Correction: Small GTPases and BAR domain proteins regulate branched actin polymerisation for clathrin and dynamin-independent endocytosis

  2018cites this paper
• Wnt/PCP controls spreading of Wnt/β-catenin signals by cytonemes in vertebrates

  2018cites this paper
• JCB_201705113 3405..3422

  2017cites this paper
• Deciphering the BAR code of membrane modulators

  2017cites this paper
• Functional Actin Networks under Construction: The Cooperative Action of Actin Nucleation and Elongation Factors.

  2017cites this paper
• Filopodyan: An open-source pipeline for the analysis of filopodia

  2017cites this paper
• MyTH4-FERM myosins in the assembly and maintenance of actin-based protrusions.

  2017cites this paper
• Missing-in-metastasis protein downregulates CXCR4 by promoting ubiquitylation and interaction with small Rab GTPases

  2017cites this paper
• Distinct VASP tetramers synergize in the processive elongation of individual actin filaments from clustered arrays

  2017cites this paper
• Redundant functions of I-BAR family members, IRSp53 and IRTKS, are essential for embryonic development

  2017cites this paper
• Actin assembly mechanisms at a glance

  2017cites this paper
• Small GTPases and BAR domain proteins regulate branched actin to make clathrin and dynamin independent endocytic vesicles

  2017cites this paper
• Identification and characterization of MicroRNAs modulating cardiac hypertrophy

  2017cites this paper