Precision genome editing: a small revolution for glycobiology.

No abstract is available for this paper.

• Publication year

  2014

• Venue

  Glycobiology

• Publication date

  2014-08-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1093/glycob/cwu046PMID 24861053
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Genome engineering using CRISPR-Cas9 system.

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• Broad Specificity Profiling of TALENs Results in Engineered Nucleases With Improved DNA Cleavage Specificity

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• High-efficiency genome editing via 2A-coupled co-expression of fluorescent proteins and zinc finger nucleases or CRISPR/Cas9 nickase pairs

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• Cell-Penetrating Peptide-Mediated Delivery of TALEN Proteins via Bioconjugation for Genome Engineering

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• Genome engineering with targetable nucleases.

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• Abstract 3482: A robust TALENs system for highly efficient mammalian genome editing

  2014cited by this paper
• Surrogate reporter-based enrichment of cells containing RNA-guided Cas9 nuclease-induced mutations

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• High frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells

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• megaTALs: a rare-cleaving nuclease architecture for therapeutic genome engineering

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• High-throughput profiling of off-target DNA cleavage reveals RNA-programmed Cas9 nuclease specificity

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• Erratum: Reading Frame correction by targeted genome editing restores dystrophin expression in cells from duchenne muscular dystrophy patients (Molecular Therapy (2013) 21 (1718-1726) DOI: 10.1038/mt.2013.111)

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