Approaches to semi-synthetic minimal cells: a review

No abstract is available for this paper.

• Publication year

  2005

• Venue

  Die Naturwissenschaften

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine, Materials Science

• Identifiers
  DOI 10.1007/s00114-005-0056-zPMID 16292523
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Life: In search of the simplest cell

  2005cited by this paper
• The evolutionary transition from RNA to DNA in early cells

  2005cited by this paper
• A model for the origin of life

  2005cited by this paper
• Cell‐free Protein Synthesis through Solubilisate Exchange in Water/Oil Emulsion Compartments

  2004influential reference
• A vesicle bioreactor as a step toward an artificial cell assembly.

  2004influential reference
• Determination of the Core of a Minimal Bacterial Gene Set

  2004cited by this paper
• A Possible Route to Prebiotic Vesicle Reproduction

  2004cited by this paper
• Expression of a cascading genetic network within liposomes

  2004cited by this paper
• Comparative Genomics and the Gene Complement of a Minimal Cell

  2004influential reference
• Nucleic-acid-templated synthesis as a model system for ancient translation.

  2004cited by this paper
• Microtubule self-organisation by reaction-diffusion processes causes collective transport and organisation of cellular particles

  2004cited by this paper
• Novel properties of DDAB: Matrix effect and interaction with oleate

  2004cited by this paper
• Gene Expression within Cell‐Sized Lipid Vesicles

  2003cited by this paper
• Principles of cell-free genetic circuit assembly

  2003cited by this paper
• Cooperative Micelle Binding and Matrix Effect in Oleate Vesicle Formation

  2003cited by this paper
• Gene cloning expression and purification of membrane proteins

  2003cited by this paper
• Autopoiesis: a review and a reappraisal

  2003cited by this paper
• Liposome-entrapped Polymerases as Models for Microscale/Nanoscale Bioreactors

  2003cited by this paper
• Tinker, Tailor: Can Venter Stitch Together a Genome From Scratch?

  2003cited by this paper
• Membrane tube formation from giant vesicles by dynamic association of motor proteins

  2003cited by this paper
• Self-organized pattern formation in motor-microtubule mixtures.

  2003cited by this paper
• Comparative genomics, minimal gene-sets and the last universal common ancestor

  2003influential reference
• Artificial cells: prospects for biotechnology.

  2002cited by this paper
• Toward the engineering of minimal living cells

  2002cited by this paper
• Extreme genome reduction in Buchnera spp.: Toward the minimal genome needed for symbiotic life

  2002cited by this paper
• The Use of Liposomes for Constructing Cell Models

  2002influential reference
• Engineering a reduced Escherichia coli genome.

  2002influential reference
• Giant Vesicles as Microreactors for Enzymatic mRNA Synthesis

  2002cited by this paper
• Chemical Synthesis of Poliovirus cDNA: Generation of Infectious Virus in the Absence of Natural Template

  2002cited by this paper
• The Notion of a DNA Minimal Cell: A General Discourse and Some Guidelines for an Experimental Approach

  2002influential reference
• A self-replicating ligase ribozyme

  2002cited by this paper
• Synthesis of functional protein in liposome.

  2001influential reference
• Growth and Transformation of Vesicles Studied by Ferritin Labeling and Cryotransmission Electron Microscopy

  2001cited by this paper
• DNA primases.

  2001cited by this paper
• Giant Liposome as a Biochemical Reactor: Transcription of DNA and Transportation by Laser Tweezers

  2001cited by this paper
• A minimal system allowing tubulation with molecular motors pulling on giant liposomes

  2001cited by this paper
• Synthesizing life

  2001cited by this paper
• The structural basis of ribosome activity in peptide bond synthesis.

  2000cited by this paper
• Self-maintenance and self-reproduction in an abstract cell model.

  2000cited by this paper
• How many genes can make a cell: the minimal-gene-set concept.

  2000cited by this paper
• Global transposon mutagenesis and a minimal Mycoplasma genome.

  1999influential reference
• The minimal genome concept.

  1999cited by this paper
• A Matrix Effect in Mixed Phospholipid/Fatty Acid Vesicle Formation

  1999cited by this paper
• Directly Observed Membrane Fusion Between Oppositely Charged Phospholipid Bilayers

  1999cited by this paper
• Protein expression in liposomes.

  1999influential reference
• Matrix Effect in the Size Distribution of Fatty Acid Vesicles

  1998cited by this paper
• Structure and Sizes of Genomes of the Archaea and Bacteria

  1998cited by this paper
• Peptidyl-transferase ribozymes: trans reactions, structural characterization and ribosomal RNA-like features.

  1998cited by this paper
• A minimal gene set for cellular life derived by comparison of complete bacterial genomes.

  1996influential reference
• INTERACTION, LIPID EXCHANGE, AND EFFECT OF VESICLE SIZE IN SYSTEMS OF OPPOSITELY CHARGED VESICLES

  1996cited by this paper
• Enzymatic RNA replication in self-reproducing vesicles: an approach to a minimal cell.

  1995cited by this paper
• An estimation of minimal genome size required for life

  1995cited by this paper
• Polymerase chain reaction in liposomes.

  1995influential reference
• The Minimal Gene Complement of Mycoplasma genitalium

  1995cited by this paper
• Production of RNA by a polymerase protein encapsulated within phospholipid vesicles

  1994cited by this paper
• OPARIN'S REACTIONS REVISITED : ENZYMATIC SYNTHESIS OF POLY(ADENYLIC ACID) IN MICELLES AND SELF-REPRODUCING VESICLES

  1994influential reference
• Autopoietic Self-Reproduction of Fatty Acid Vesicles

  1994cited by this paper
• On the early emergence of reverse transcription: Theoretical basis and experimental evidence

  1992cited by this paper
• Autocatalytic self-replicating micelles as models for prebiotic structures

  1992cited by this paper
• Liposome-mediated enzymatic synthesis of phosphatidylcholine as an approach to self-replicating liposomes

  1991cited by this paper
• Self-replicating micelles — A chemical version of a minimal autopoietic system

  1989cited by this paper
• Interactions of cationic lipid vesicles with negatively charged phospholipid vesicles and biological membranes.

  1988cited by this paper
• Basic protein enhances the incorporation of DNA into lipid vesicles: model for the formation of primordial cells.

  1987cited by this paper
• tRNA-like structures tag the 3' ends of genomic RNA molecules for replication: implications for the origin of protein synthesis.

  1987cited by this paper
• Ribosome structure and protein biosynthesis

  1986cited by this paper
• Factor-free ("non-enzymic") and factor-dependent systems of translation of polyuridylic acid by Escherichia coli ribosomes.

  1976cited by this paper
• Autopoiesis: the organization of living systems, its characterization and a model.

  1974cited by this paper
• The effects of initiation factor 3 on the formation of 30S initiation complexes with synthetic and natural messengers.

  1974cited by this paper
• Biological Self-Replicating Systems*

  1967cited by this paper

• Advances in artificial cells capable of metabolic mimicry: from fundamentals to applications.

  2026cites this paper
• Evolution of pre-genetic semantic information in protocells under variable inheritance fidelity

  2026cites this paper
• Nature-Inspired Enzymatic Cascades: Emerging Strategies for Sustainable Chemistry.

  2026cites this paper
• Template-Based Catalysis and the Emergence of Collectively Autocatalytic Systems.

  2026cites this paper
• Synthetic cells by the numbers

  2025cites this paper
• Autonomous biogenesis of all thirty proteins of the Escherichia coli translation machinery

  2025cites this paper
• Division Strategies Determine Growth and Viability of Growing-Dividing Autocatalytic Systems

  2025cites this paper
• Biomanufacturing Potential of Streamlined Cells

  2025cites this paper
• Generalist versus Specialist Self-Replicators.

  2024cites this paper
• Evolutionary Abilities of Minimalistic Physicochemical Models of Life Processes.

  2024cites this paper
• Synthetic cell research: Is technical progress leaving theoretical and epistemological investigations one step behind?

  2023cites this paper
• Modelling spatial constraints and scaling effects of catalyst phase separation on linear pathway kinetics

  2023cites this paper
• A Hitchhiker’s Guide to Supplying Enzymatic Reducing Power into Synthetic Cells

  2023cites this paper
• A Role for Bottom-Up Synthetic Cells in the Internet of Bio-Nano Things?

  2023cites this paper
• DNA droplets for intelligent and dynamical artificial cells: from the viewpoint of computation and non-equilibrium systems

  2023cites this paper
• Cell-sized asymmetric phospholipid-amphiphilic protein vesicles with growth, fission, and molecule transportation

  2023cites this paper
• Collective catalysis under spatial constraints: Phase separation and size-scaling effects on mass action kinetics.

  2023cites this paper
• Chemical Reactions in Living Systems

  2023cites this paper
• Explorative Synthetic Biology in AI: Criteria of Relevance and a Taxonomy for Synthetic Models of Living and Cognitive Processes

  2023cites this paper
• Advancing synthetic biology through cell-free protein synthesis

  2023cites this paper
• Applications of vesicle-based artificial cells in analytical chemistry: A review

  2023cites this paper
• Gene‐Directed FtsZ Ring Assembly Generates Constricted Liposomes with Stable Membrane Necks

  2023cites this paper
• New Aequorea fluorescent proteins for cell-free bioengineering

  2022cites this paper
• Synthetic cells with self-activating optogenetic proteins communicate with natural cells

  2022cites this paper
• Artificial Cells: Past, Present and Future.

  2022cites this paper
• Printing and Erasing of DNA‐Based Photoresists Inside Synthetic Cells

  2022cites this paper
• Programmatically Dynamic Microcompartmentation in Coacervate-in-Pickering Emulsion Protocell.

  2022cites this paper
• The fabrication of phospholipid vesicle-based artificial cells and their functions

  2021cites this paper
• A large, square-shaped, DNA origami nanopore with sealing function on a giant vesicle membrane.

  2021cites this paper
• Bioluminescent Synthetic Cells Communicate with Natural Cells and Self-Activate Light-Responsive Proteins

  2021cites this paper
• Minimal Pathway for the Regeneration of Redox Cofactors

  2021cites this paper
• Thermoacoustic Instability Is Self-Organization in a Complex System

  2021cites this paper
• Bioinspired Networks of Communicating Synthetic Protocells

  2021cites this paper
• Synthetic Cells Engaged in Molecular Communication: An Opportunity for Modelling Shannon- and Semantic-Information in the Chemical Domain

  2021cites this paper
• Shaping Liposomes by Cell-Free Expressed Bacterial Microtubules

  2021cites this paper
• Exploiting the photoactivity of bacterial reaction center to investigate liposome dynamics

  2021cites this paper
• Dreaming of a Universal Biology: Synthetic Biology and the Origins of Life

  2021cites this paper
• Multivesicular Vesicles: Preparation and Applications

  2021cites this paper
• Optimizing Enzymatic Photo‐Redox Cycles by a Hybrid Protein Complex Chain

  2020cites this paper
• Cell-Free Systems: A Proving Ground for Rational Biodesign

  2020cites this paper
• The Metabolic Building Blocks of a Minimal Cell

  2020cites this paper
• Roadmap to Building a Cell: An Evolutionary Approach

  2020cites this paper
• On the “Life-Likeness” of Synthetic Cells

  2020cites this paper
• A dynamic microarray device for pairing and electrofusion of giant unilamellar vesicles

  2020cites this paper
• Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer.

  2020cites this paper
• Self-division of giant vesicles driven by an internal enzymatic reaction

  2020cites this paper
• Bioinspired Protein‐Based Assembling: Toward Advanced Life‐Like Behaviors

  2020cites this paper
• Autonomous synthesis and assembly of a ribosomal subunit on a chip

  2020cites this paper
• Genetically controlled membrane synthesis in liposomes

  2020cites this paper
• Minimal Genomes: How Many Genes Does a Cell Require To Be Viable

  2020cites this paper
• Interfacing Living and Synthetic Cells as an Emerging Frontier in Synthetic Biology

  2020cites this paper
• Novel droplet ripening behaviour in ternary oil-alcohol-water systems

  2019cites this paper
• Genetically Encoded Membranes for Bottom‐Up Biology

  2019cites this paper
• Pairing and Electrofusion of Liposomes in a Dynamic Microarray Device

  2019cites this paper
• A synthetic metabolic network for physicochemical homeostasis

  2019cites this paper
• Gene Expression Inside Liposomes: From Early Studies to Current Protocols.

  2019cites this paper
• Synthetic biology

  2019cites this paper
• Polyelectrolyte-micelle coacervates: intrapolymer-dominant vs. interpolymer-dominant association, solute uptake and rheological properties.

  2019cites this paper
• Modelling a Light Transducing Protocell Population

  2019cites this paper
• Establishing communication between artificial cells.

  2019cites this paper
• Why High-Performance Modelling and Simulation for Big Data Applications Matters

  2019cites this paper
• A protocell with fusion and division.

  2019cites this paper
• Artificial Cells Capable of Long-Lived Protein Synthesis by Using Aptamer Grafted Polymer Hydrogel.

  2019cites this paper
• What can we learn from the construction of in vitro replication systems?

  2019cites this paper
• Annual Congress of the Italian Society of Photobiology – SIFB2019

  2019cites this paper
• Glycan-decorated protocells: novel features for rebuilding cellular processes

  2019cites this paper
• Molecular Communications in the Context of “Synthetic Cells” Research

  2019cites this paper
• Novel chassis for engineered pathways and automating biological experiments in the biotechnology industry

  2019cites this paper
• Gene-Expressing Liposomes as Synthetic Cells for Molecular Communication Studies

  2019cites this paper
• Synthetic Biology and Artificial Intelligence: Grounding a Cross-Disciplinary Approach to the Synthetic Exploration of (Embodied) Cognition

  2018cites this paper
• Synthetic Biology and Artificial Intelligence: Toward Cross-Fertilization

  2018cites this paper
• Long-lived protein expression in hydrogel particles: towards artificial cells† †Electronic supplementary information (ESI) available: Experimental details and results. See DOI: 10.1039/c8sc00383a

  2018cites this paper
• Synthetic biology and (embodied) artificial intelligence: opportunities and challenges

  2018cites this paper
• High-Throughput Optimization Cycle of a Cell-Free Ribosome Assembly and Protein Synthesis System.

  2018cites this paper
• Constructing vesicle-based artificial cells with embedded living cells as organelle-like modules

  2018cites this paper
• Functionalizing cell-mimetic giant vesicles with encapsulated bacterial biosensors

  2018cites this paper
• Cell-Free Approaches in Synthetic Biology Utilizing Microfluidics

  2018cites this paper
• Hybrid cell reactor system from Escherichia coli protoplast cells and arrayed lipid bilayer chamber device

  2018cites this paper
• Engineering thermoresponsive phase separated vesicles formed via emulsion phase transfer as a content-release platform

  2018cites this paper
• Copy, paste, repeat — über die Synthese von Minimalzellen

  2018cites this paper
• Current Directions in Synthetic Cell Research

  2018cites this paper
• Comparative genomics of 151 plant-associated bacteria reveal putative mechanisms underlying specific interactions between bacteria and plant hosts

  2018cites this paper
• Synthetic Cells Synthesize Therapeutic Proteins inside Tumors

  2018cites this paper
• General Introduction and Nanoscale View of the Cell

  2018cites this paper
• The System View of the Origin of Cellular Life

  2018cites this paper
• Modelling Giant Lipid Vesicles Designed for Light Energy Transduction

  2018cites this paper
• Why on Theoretical Grounds It Is Likely that “Life” Exists Throughout the universe

  2018cites this paper
• Measurement and Numerical Modeling of Cell-Free Protein Synthesis: Combinatorial Block-Variants of the PURE System

  2018cites this paper
• Is Research on “Synthetic Cells” Moving to the Next Level?

  2018cites this paper
• Self-replication of DNA by its encoded proteins in liposome-based synthetic cells

  2018cites this paper
• Synthetic cells produce a quorum sensing chemical signal perceived by Pseudomonas aeruginosa.

  2018cites this paper
• De Novo Synthesis of Basal Bacterial Cell Division Proteins FtsZ, FtsA, and ZipA Inside Giant Vesicles.

  2018cites this paper
• Do protocells preferentially retain macromolecular solutes upon division/fragmentation? A study based on the extrusion of POPC giant vesicles.

  2018cites this paper
• Building Biomimetic Systems using Vesicles and DNA Channels

  2018cites this paper
• Extrinsic stochastic factors (solute partition) in gene expression inside lipid vesicles and lipid-stabilized water-in-oil droplets: a review

  2018cites this paper
• Membrane-mediated transport in a non-equilibrium hybrid protocell based on coacervate droplets and a surfactant.

  2018cites this paper
• Flow Cytometric Analysis To Evaluate Morphological Changes in Giant Liposomes As Observed in Electrofusion Experiments.

  2018cites this paper
• Self-Assembly and Primitive Membrane Formation: Between Stability and Dynamism

  2018cites this paper
• Highly oriented photosynthetic reaction centers generate a proton gradient in synthetic protocells

  2017cites this paper
• The Role of Lipid Membranes in Life’s Origin

  2017cites this paper