Identification of the endocytic sorting signal recognized by the Art1-Rsp5 ubiquitin ligase complex

Evan L. Guiney,Till Klecker,S. Emr

Published 2016 in Molecular Biology of the Cell

ABSTRACT

Endocytosis of plasma membrane proteins in Saccharomyces cerevisiae requires their ubiquitination by the ART-Rsp5 ubiquitin ligase complex. Little is known about how the complex engages substrates. The Art1 C-terminus recognizes Mup1 via a tripartite ART sorting signal: an acidic patch, in proximity to the membrane and substrate lysines.

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