Nup180, a novel nuclear pore complex protein localizing to the cytoplasmic ring and associated fibrils

Using an autoimmune serum from a patient with overlap connective tissue disease we have identified by biochemical and immunocytochemical approaches an evolutionarily conserved nuclear pore complex (NPC) protein with an estimated molecular mass of 180 kD and an isoelectric point of approximately 6.2 which we have designated as nup180. Extraction of isolated nuclear envelopes with 2 M urea and chromatography of the solubilized proteins on WGA-Sepharose demonstrated that nup180 is a peripheral membrane protein and does not react with WGA. Affinity-purified antibodies yielded a punctate immunofluorescent pattern of the nuclear surface of mammalian cells and stained brightly the nuclear envelope of cryosectioned Xenopus oocytes. Nuclei reconstituted in vitro in Xenopus egg extract were also stained in the characteristic punctate fashion. Immunogold EM localized nup180 exclusively to the cytoplasmic ring of NPCs and short fibers emanating therefrom into the cytoplasm. Antibodies to nup180 did not inhibit nuclear protein transport in vivo nor in vitro. Despite the apparent lack of involvement in NPC assembly or nucleocytoplasmic transport processes, the conservation of nup180 across species and its exclusive association with the NPC cytoplasmic ring suggests an important, though currently undefined function for this novel NPC protein.

• Publication year

  1993

• Venue

  Journal of Cell Biology

• Publication date

  1993-12-02

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/JCB.123.6.1345PMID 8253835PMCID 2290874
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The nuclear pore complex

  1993cited by this paper
• The nuclear pore complex and nucleocytoplasmic transport.

  1993cited by this paper
• Architecture of the Xenopus nuclear pore complex revealed by three- dimensional cryo-electron microscopy

  1993cited by this paper
• Nup155 is a novel nuclear pore complex protein that contains neither repetitive sequence motifs nor reacts with WGA

  1993influential reference
• A nuclear pore complex protein that contains zinc finger motifs, binds DNA, and faces the nucleoplasm.

  1993cited by this paper
• 7 – Structure and Function of Nuclear Pore Glycoproteins

  1992cited by this paper
• High resolution scanning electron microscopy of the nuclear envelope: demonstration of a new, regular, fibrous lattice attached to the baskets of the nucleoplasmic face of the nuclear pores

  1992cited by this paper
• Structure and function of the nuclear pore complex.

  1992cited by this paper
• In vitro assembly of prenucleolar bodies in Xenopus egg extract

  1992influential reference
• Nucleocytoplasmic traffic of proteins.

  1992cited by this paper
• Nuclear pore structure and function.

  1992cited by this paper
• 4 – Regulation of Nucleocytoplasmic Transport by Mechanical Forces Transmitted Through the Cytoskeleton

  1992cited by this paper
• Architecture and design of the nuclear pore complex.

  1992cited by this paper
• Export of mRNA from microinjected nuclei of Xenopus laevis oocytes

  1992cited by this paper
• Autoantibodies to major and minor nuclear lamins are not restricted to autoimmune diseases.

  1992cited by this paper
• Pathways for the nuclear transport of proteins and RNAs.

  1991cited by this paper
• Nuclear pore complex glycoprotein p62 of Xenopus laevis and mouse: cDNA cloning and identification of its glycosylated region.

  1991cited by this paper
• Autoantibodies to DNA, lamins, and pore complex proteins produce distinct peripheral fluorescent antinuclear antibody patterns on the HEp-2 substrate.

  1991cited by this paper
• Autoantibodies in pathology and cell biology.

  1991cited by this paper
• Toward a more complete 3-D structure of the nuclear pore complex.

  1991cited by this paper
• Spontaneous assembly of pore complex-containing membranes ("annulate lamellae") in Xenopus egg extract in the absence of chromatin

  1991cited by this paper
• Autoantibodies against nuclear envelope proteins in liver disease

  1991cited by this paper
• Localization of a myosin heavy chain-like polypeptide to Drosophila nuclear pore complexes.

  1991cited by this paper
• Identification of a soluble precursor complex essential for nuclear pore assembly in vitro

  1990cited by this paper
• Cytoplasmic transport of ribosomal subunits microinjected into the Xenopus laevis oocyte nucleus: a generalized, facilitated process

  1990cited by this paper
• Assembly of the cell nucleus.

  1990cited by this paper
• Correlation between structure and mass distribution of the nuclear pore complex and of distinct pore complex components

  1990cited by this paper
• A major glycoprotein of the nuclear pore complex is a membrane‐spanning polypeptide with a large lumenal domain and a small cytoplasmic tail.

  1990cited by this paper
• Interactions of intermediate filaments with cell structures.

  1990cited by this paper
• The NUP1 gene encodes an essential component of the yeast nuclear pore complex.

  1990cited by this paper
• Interaction of intermediate filaments with nuclear lamina and cell periphery.

  1989cited by this paper
• Functional role of newly formed pore complexes in postmitotic nuclear reorganization

  1989cited by this paper
• Antinuclear antibodies: diagnostic markers for autoimmune diseases and probes for cell biology.

  1989cited by this paper
• Protein import through the nuclear pore complex is a multistep process

  1989cited by this paper
• Primary structure analysis of an integral membrane glycoprotein of the nuclear pore

  1989cited by this paper
• Interactions and structure of the nuclear pore complex revealed by cryo- electron microscopy

  1989cited by this paper
• Inhibition of nuclear accumulation of karyophilic proteins in living cells by microinjection of the lectin wheat germ agglutinin.

  1988cited by this paper
• A novel autoantibody causing a peripheral fluorescent antinuclear antibody pattern is specific for nuclear pore complexes.

  1988cited by this paper
• Monoclonal antibodies to a Mr 68000 pore complex glycoprotein interfere with nuclear protein uptake in Xenopus oocytes

  1988cited by this paper
• Nuclear protein migration involves two steps: rapid binding at the nuclear envelope followed by slower translocation through nuclear pores.

  1988cited by this paper
• Filamentous cross-bridges link intermediate filaments to the nuclear pore complexes.

  1988cited by this paper
• Nuclear import can be separated into distinct steps in vitro: nuclear pore binding and translocation.

  1988cited by this paper
• The reconstitution of nuclear envelopes in cell-free extracts.

  1988cited by this paper
• The nuclear envelope and the organization of the pore complexes.

  1988cited by this paper
• Translocation of RNA-coated gold particles through the nuclear pores of oocytes

  1988cited by this paper
• A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in vivo

  1988cited by this paper
• Monoclonal autoantibody from a (New Zealand black x New Zealand white)F1 mouse and some human scleroderma sera target an Mr 34,000 nucleolar protein of the U3 RNP particle.

  1987cited by this paper
• Monoclonal antibodies identify a group of nuclear pore complex glycoproteins

  1987cited by this paper
• Nuclear reconstitution in vitro: stages of assembly around protein-free DNA.

  1987cited by this paper
• Reversible inhibition of protein import into the nucleus by wheat germ agglutinin injected into cultured cells.

  1987cited by this paper
• Inhibition of in vitro nuclear transport by a lectin that binds to nuclear pores

  1987cited by this paper
• A monoclonal antibody against a family of nuclear pore proteins (nucleoporins): O-linked N-acetylglucosamine is part of the immunodeterminant.

  1987cited by this paper
• O-linked N-acetylglucosamine is attached to proteins of the nuclear pore. Evidence for cytoplasmic and nucleoplasmic glycoproteins.

  1987cited by this paper
• Initiation of DNA replication in nuclei and purified DNA by a cell-free extract of Xenopus eggs.

  1986cited by this paper
• Identification and characterization of a nuclear pore complex protein

  1986cited by this paper
• Cell type-specific expression of nuclear lamina proteins during development of Xenopus laevis.

  1985cited by this paper
• Movement of a karyophilic protein through the nuclear pores of oocytes

  1984cited by this paper
• Electroblotting of multiple gels: a simple apparatus without buffer tank for rapid transfer of proteins from polyacrylamide to nitrocellulose.

  1984cited by this paper
• Organization and Modulation of Nuclear Lamina Structure

  1984cited by this paper
• Proteins of pore complex--lamina structures from nuclei and nuclear membranes.

  1983cited by this paper
• A large particle associated with the perimeter of the nuclear pore complex

  1982cited by this paper
• Identification of a major polypeptide of the nuclear pore complex

  1982influential reference
• The nuclear envelope and the architecture of the nuclear periphery

  1981cited by this paper
• Cell type-specific differences in protein composition of nuclear pore complex-lamina structures in oocytes and erythrocytes of Xenopus laevis.

  1981cited by this paper
• The major polypeptides of the nuclear pore complex.

  1978cited by this paper
• The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution.

  1977cited by this paper
• The nuclear and the cytoplasmic pore complex: structure, dynamics, distribution, and evolution.

  1977cited by this paper
• An octamer of histones in chromatin and free in solution.

  1975influential reference
• High resolution two-dimensional electrophoresis of proteins.

  1975cited by this paper
• 5 – Structures and Functions of the Nuclear Envelope

  1974cited by this paper
• Structure and Function of the Nuclear Envelope and Related Cytomembranes

  1973cited by this paper
• The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. I. The mature oocyte.

  1970cited by this paper

• An Autoimmune Myositis-Overlap Syndrome Associated With Autoantibodies to Nuclear Pore Complexes

  2014cites this paper
• Heterogeneity of autoantibodies in 100 patients with autoimmune myositis: insights into clinical features and outcomes

  2007cites this paper
• Passive and Facilitated Transport in Nuclear Pore Complexes Is Largely Uncoupled*

  2006cites this paper
• Nuclear envelopes from amphibian oocytes--from morphology to protein inventory.

  2005cites this paper
• Emerin expression in early development of Xenopus laevis.

  2005cites this paper
• Autoantigens of the nuclear pore complex

  2004cites this paper
• Nuclear pore membrane glycoprotein 210 as a new marker for epithelial cells

  2003cites this paper
• Autoantibodies to NOR 90/hUBF: longterm clinical and serological followup in a patient with limited systemic sclerosis suggests an antigen driven immune response.

  2002cites this paper
• Nic 96 p Is Required for Nuclear Pore Formation and Functionally Interacts with a Novel Nucleoporin , Nup 188 p

  2002cites this paper
• Traffic Jam: A Compendium of Human Diseases that Affect Intracellular Transport Processes

  2000cites this paper
• Cellular uptake and nuclear delivery of recombinant adenovirus penton base.

  1999cites this paper
• Signals mediating nuclear targeting and their regulation: Application in drug delivery

  1998cites this paper
• Mapping proteins to nuclear pore complexes by immunogold electron microscopy.

  1998cites this paper
• Molecular mechanisms of nuclear protein transport.

  1997cites this paper
• Three-dimensional visualization of the route of protein import: the role of nuclear pore complex substructures.

  1997cites this paper
• Nuclear envelope protein autoantibodies in primary biliary cirrhosis.

  1997cites this paper
• A role for the divergent actin gene, ACT2, in nuclear pore structure and function

  1997cites this paper
• Chromosome behaviour in earliest meiotic prophase

  1997cites this paper
• Nuclear pore complex structure in birds.

  1997cites this paper
• Preassembly of annulate lamellae in egg extracts inhibits nuclear pore complex formation, but not nuclear membrane assembly.

  1997cites this paper
• Nuclear Envelope Protein Autoantibodies in Primary Biliary Cirrhosis

  1997cites this paper
• Molecular dissection of the nuclear pore complex.

  1996cites this paper
• Cytoplasmic annulate lamellae in cultured cells: composition, distribution, and mitotic behavior

  1996cites this paper
• CD4-reactive antibodies in systemic lupus erythematosus.

  1996cites this paper
• Regulation of protein transport to the nucleus: central role of phosphorylation.

  1996cites this paper
• Nic96p is required for nuclear pore formation and functionally interacts with a novel nucleoporin, Nup188p

  1996influential citation
• Accessing nuclear structure for field emission, in lens, scanning electron microscopy (FEISEM).

  1996cites this paper
• The nuclear pore complex and lamina: three-dimensional structures and interactions determined by field emission in-lens scanning electron microscopy.

  1996cites this paper
• A biochemical and immunological comparison of nuclear and cytoplasmic pore complexes.

  1996cites this paper
• The Meaning of Nucleocytoplasmic Transport

  1996cites this paper
• Product of the oncogene‐activating gene Tpr is a phosphorylated protein of the nuclear pore complex

  1996cites this paper
• The nuclear pore complex: Toward its molecular architecture, structure, and function

  1996cites this paper
• Nuclear Envelope Protein Autoantibodies

  1996cites this paper
• Cellular Structures and Nucleocytoplasmic Transport

  1996cites this paper
• Toward a molecular understanding of the structure and function of the nuclear pore complex.

  1995cites this paper
• Structural and functional organization of the nuclear envelope.

  1995cites this paper
• Immunolocalization of lamins and nuclear pore complex proteins by atomic force microscopy

  1995cites this paper
• Nuclear transport of 5S ribosomal RNA in Xenopus laevis oocytes.

  1995cites this paper
• Nuclear pore complex proteins.

  1995cites this paper
• Characterization of a novel human IgG antibody reactive with a Ca(2+)-sensitive cell-cell adhesion epitope of PtK2 epithelial cells.

  1995cites this paper
• Hypergammaglobulinemic purpura in systemic autoimmune rheumatic diseases: predictive value of anti-Ro(SSA) and anti-La(SSB) antibodies and treatment with indomethacin and hydroxychloroquine.

  1995cites this paper
• Nuclear transport of uracil-rich small nuclear ribonucleoprotein particles

  1995cites this paper
• Reconstituted nuclei depleted of a vertebrate GLFG nuclear pore protein, p97, import but are defective in nuclear growth and replication

  1995cites this paper
• Functional interaction of Nic96p with a core nucleoporin complex consisting of Nsp1p, Nup49p and a novel protein Nup57p.

  1995cites this paper
• Localization of the Ran-GTP binding protein RanBP2 at the cytoplasmic side of the nuclear pore complex.

  1995cites this paper
• Characterization of a novel human antibody xenoreactive with fibronectin.

  1995cites this paper
• Structural interaction between the nuclear pore complex and a specific translocating RNP particle

  1995cites this paper
• Signalling and transport through the nuclear membrane.

  1995cites this paper
• Molecular characterization of components of the nuclear pore complex and the nuclear import system in Saccharomyces cerevisiae

  1995cites this paper
• Nuclear pore complex assembly studied with a biochemical assay for annulate lamellae formation

  1995cites this paper
• Unidirectional and bidirectional protein traffic across membranes.

  1995cites this paper
• Export of mRNA Through the Nuclear Pore Complex

  1995cites this paper
• The nuclear pore complex.

  1995cites this paper
• Anti-microfilament IgG antibodies in normal adults and in patients with autoimmune diseases: immunofluorescence and immunoblotting analysis of 201 subjects reveals polyreactivity with microfilament-associated proteins.

  1995cites this paper
• High content of a nuclear pore complex protein in cytoplasmic annulate lamellae of Xenopus oocytes.

  1995cites this paper
• Plant nuclear pore complex proteins are modified by novel oligosaccharides with terminal N-acetylglucosamine.

  1995cites this paper
• Interactions and three-dimensional localization of a group of nuclear pore complex proteins

  1994influential citation
• Tpr, a large coiled coil protein whose amino terminus is involved in activation of oncogenic kinases, is localized to the cytoplasmic surface of the nuclear pore complex

  1994cites this paper
• Nuclear transport.

  1994cites this paper
• Toward the molecular details of the nuclear pore complex.

  1994cites this paper
• Towards understanding the three-dimensional structure of the nuclear pore complex at the molecular level

  1994cites this paper
• Pores for thought: nuclear pore complex proteins.

  1994cites this paper
• Polymyositis presenting as bilateral trigeminal neuropathy.

  1994cites this paper
• The nuclear pore complex

  1993cites this paper
• Intranuclear filaments containing a nuclear pore complex protein

  1993cites this paper