Dynamic reorganization of the actin cytoskeleton

Cellular processes, including morphogenesis, polarization, and motility, rely on a variety of actin-based structures. Although the biochemical composition and filament organization of these structures are different, they often emerge from a common origin. This is possible because the actin structures are highly dynamic. Indeed, they assemble, grow, and disassemble in a time scale of a second to a minute. Therefore, the reorganization of a given actin structure can promote the formation of another. Here, we discuss such transitions and illustrate them with computer simulations.

• Publication year

  2015

• Venue

  F1000Research

• Publication date

  2015-10-01

• Fields of study

  Biology, Medicine, Physics, Computer Science

• Identifiers
  DOI 10.12688/f1000research.6374.1PMID 26989473PMCID 4784019
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Faculty Opinions recommendation of Molecular mechanism of Ena/VASP-mediated actin-filament elongation.

  2015cited by this paper
• Inverted formin 2 in focal adhesions promotes dorsal stress fiber and fibrillar adhesion formation to drive extracellular matrix assembly

  2015cited by this paper
• Profilin-1 serves as a gatekeeper for actin assembly by Arp2/3-dependent and -independent pathways.

  2015cited by this paper
• Profilin regulates F-actin network homeostasis by favoring formin over Arp2/3 complex.

  2015cited by this paper
• A mechanism of leading-edge protrusion in the absence of Arp2/3 complex

  2015cited by this paper
• F-actin bundles direct the initiation and orientation of lamellipodia through adhesion-based signaling

  2015cited by this paper
• Geometrical and Mechanical Properties Control Actin Filament Organization

  2014cited by this paper
• Ena/VASP Enabled is a highly processive actin polymerase tailored to self-assemble parallel-bundled F-actin networks with Fascin

  2014cited by this paper
• Capping protein regulators fine-tune actin assembly dynamics

  2014cited by this paper
• Actin dynamics, architecture, and mechanics in cell motility.

  2014cited by this paper
• Capping protein regulators fine-tune actin assembly dynamics

  2014cited by this paper
• Homeostatic actin cytoskeleton networks are regulated by assembly factor competition for monomers.

  2014cited by this paper
• Rac function is crucial for cell migration but is not required for spreading and focal adhesion formation

  2013cited by this paper
• GMF severs actin-Arp2/3 complex branch junctions by a cofilin-like mechanism.

  2013cited by this paper
• Structural features and interfacial properties of WH2, β‐thymosin domains and other intrinsically disordered domains in the regulation of actin cytoskeleton dynamics

  2013cited by this paper
• The Arp2/3 complex is required for lamellipodia extension and directional fibroblast cell migration

  2012cited by this paper
• Arp2/3 is critical for lamellipodia and response to extracellular matrix cues but is dispensable for chemotaxis.

  2012cited by this paper
• Theory of active transport in filopodia and stereocilia

  2012cited by this paper
• Physical model for self-organization of actin cytoskeleton and adhesion complexes at the cell front.

  2012cited by this paper
• Actin stress fibers – assembly, dynamics and biological roles

  2012cited by this paper
• Filopodia initiation

  2011cited by this paper
• Molecular mechanism of Ena/VASP-mediated actin-filament elongation

  2011cited by this paper
• A role for actin arcs in the leading-edge advance of migrating cells

  2011cited by this paper
• Stress fibers stabilize the position of intranuclear DNA through mechanical connection with the nucleus in vascular smooth muscle cells

  2011cited by this paper
• Cofilin cooperates with fascin to disassemble filopodial actin filaments

  2011cited by this paper
• Forming the cell rear first: breaking cell symmetry to trigger directed cell migration

  2010cited by this paper
• Protrusion and actin assembly are coupled to the organization of lamellar contractile structures.

  2010cited by this paper
• Cell mechanics and the cytoskeleton

  2010cited by this paper
• A nucleator arms race: cellular control of actin assembly

  2010cited by this paper
• Nucleation geometry governs ordered actin networks structures.

  2010cited by this paper
• GMF is a cofilin homolog that binds Arp2/3 complex to stimulate filament debranching and inhibit actin nucleation.

  2010cited by this paper
• Mathematics of cell motility: have we got its number?

  2009cited by this paper
• Cofilin dissociates Arp2/3 complex and branches from actin filaments.

  2009cited by this paper
• Actin nucleation and elongation factors: mechanisms and interplay.

  2009cited by this paper
• Role of focal adhesions and mechanical stresses in the formation and progression of the lamellipodium-lamellum interface [corrected].

  2009cited by this paper
• From branched networks of actin filaments to bundles.

  2009cited by this paper
• Regulation of actin assembly associated with protrusion and adhesion in cell migration.

  2008cited by this paper
• Membrane-induced bundling of actin filaments.

  2008cited by this paper
• Differentially oriented populations of actin filaments generated in lamellipodia collaborate in pushing and pausing at the cell front

  2008cited by this paper
• Arp2/3 complex interactions and actin network turnover in lamellipodia

  2008cited by this paper
• Mechanism of shape determination in motile cells

  2008cited by this paper
• Filopodia: molecular architecture and cellular functions

  2008cited by this paper
• Retrograde flow and myosin II activity within the leading cell edge deliver F-actin to the lamella to seed the formation of graded polarity actomyosin II filament bundles in migrating fibroblasts.

  2008influential reference
• Mechanisms of actin stress fibre assembly

  2008cited by this paper
• Filopodia formation induced by active mDia2/Drf3

  2008cited by this paper
• Building the actin cytoskeleton: filopodia contribute to the construction of contractile bundles in the lamella

  2008cited by this paper
• Actin stress fibres

  2007cited by this paper
• The stochastic dynamics of filopodial growth.

  2007cited by this paper
• Collective Langevin dynamics of flexible cytoskeletal fibers

  2007cited by this paper
• Reconstitution of the transition from lamellipodium to filopodium in a membrane-free system.

  2006cited by this paper
• Filopodia formation in the absence of functional WAVE- and Arp2/3-complexes.

  2006cited by this paper
• Stress fibers are generated by two distinct actin assembly mechanisms in motile cells

  2006cited by this paper
• Faculty Opinions recommendation of Focal adhesion size controls tension-dependent recruitment of alpha-smooth muscle actin to stress fibers.

  2006cited by this paper
• Focal adhesion size controls tension-dependent recruitment of α-smooth muscle actin to stress fibers

  2006cited by this paper
• The making of filopodia.

  2006cited by this paper
• Staying in shape with formins.

  2006cited by this paper
• The Rho family GTPase Rif induces filopodia through mDia2.

  2005cited by this paper
• The physics of filopodial protrusion.

  2005cited by this paper
• The Diaphanous-related formin dDia2 is required for the formation and maintenance of filopodia

  2005cited by this paper
• Crystal structures of a Formin Homology-2 domain reveal a tethered dimer architecture.

  2004cited by this paper
• Early molecular events in the assembly of the focal adhesion-stress fiber complex during fibroblast spreading.

  2004cited by this paper
• Capping protein: new insights into mechanism and regulation.

  2004cited by this paper
• Lamellipodial versus filopodial mode of the actin nanomachinery: pivotal role of the filament barbed end.

  2004cited by this paper
• Mechanism of filopodia initiation by reorganization of a dendritic network

  2003cited by this paper
• Formin leaky cap allows elongation in the presence of tight capping proteins.

  2003cited by this paper
• Cellular motility driven by assembly and disassembly of actin filaments.

  2003cited by this paper
• Formation of filopodia-like bundles in vitro from a dendritic network

  2003cited by this paper
• Cellular control of actin nucleation.

  2002cited by this paper
• The Ena/VASP enigma

  2002cited by this paper
• An actin nucleation mechanism mediated by Bni1 and Profilin

  2002cited by this paper
• WH2 domain: a small, versatile adapter for actin monomers

  2002cited by this paper
• Role of Formins in Actin Assembly: Nucleation and Barbed-End Association

  2002cited by this paper
• Molecular mechanisms controlling actin filament dynamics in nonmuscle cells.

  2000cited by this paper
• Arp2/3 Complex and Actin Depolymerizing Factor/Cofilin in Dendritic Organization and Treadmilling of Actin Filament Array in Lamellipodia

  1999cited by this paper
• Regulated Actin Cytoskeleton Assembly at Filopodium Tips Controls Their Extension and Retraction

  1999cited by this paper
• Assembling an actin cytoskeleton for cell attachment and movement.

  1998cited by this paper
• The interaction of Arp2/3 complex with actin: nucleation, high affinity pointed end capping, and formation of branching networks of filaments.

  1998cited by this paper
• Identification of Novel Graded Polarity Actin Filament Bundles in Locomoting Heart Fibroblasts: Implications for the Generation of Motile Force

  1997cited by this paper
• Purification of a cortical complex containing two unconventional actins from Acanthamoeba by affinity chromatography on profilin-agarose

  1994cited by this paper
• Actin assembly in response to extracellular signals: role of capping proteins, thymosin beta 4 and profilin.

  1994cited by this paper
• How profilin promotes actin filament assembly in the presence of thymosin beta 4.

  1993cited by this paper
• How profilin promotes actin filament assembly in the presence of thymosin β4

  1993cited by this paper
• Actin microfilament dynamics in locomoting cells

  1991cited by this paper
• Mechanism of the interaction of human platelet profilin with actin

  1991cited by this paper
• Myosin structure and function in cell motility.

  1987cited by this paper
• Quantitative analysis of the effect of Acanthamoeba profilin on actin filament nucleation and elongation.

  1984cited by this paper
• A Side View of Moving Fibroblasts

  1969cited by this paper
• Mathematics of Cell Motility: Have We Got Its Number?

  year unknowncited by this paper

• Single power-law rheology of crowded cytoplasm in living cells

  2026cites this paper
• Differential activity of non-muscle myosin IIA and IIB isoforms generates a dynamic actomyosin network in a concentration-dependent manner.

  2026cites this paper
• Cryo-EM structure revealed a novel F-actin binding motif in a Legionella pneumophila lysine fatty acyltransferase

  2026cites this paper
• Interplay between Rac1/RhoA and actin waves in giant epithelial cells : experiment and theory

  2025cites this paper
• Single power-law rheology of crowded cytoplasm in living cells

  2025cites this paper
• Cryo-EM structure revealed a novel F-actin binding motif in a Legionella pneumophila lysine fatty-acyltransferase

  2025cites this paper
• Hackathons for biophysics education: simulating the cytoskeleton

  2025cites this paper
• Four Novel Rho‐associated Coiled‐coil Protein Kinase 1 Inhibitors Suppressing Cytoskeleton and Movement in Breast Cancer Cells

  2025cites this paper
• Geometrically constrained cytoskeletal reorganisation modulates DNA nanostructures uptake

  2025cites this paper
• Filament transport supports contractile steady states of actin networks

  2025cites this paper
• Microfluidics and 3D printed scaffolds for periodontal regeneration: A comprehensive review.

  2025cites this paper
• Demyelination and Remyelination: General Principles.

  2025cites this paper
• Incarvine C and its analogues inhibit the formation of cell cytoskeleton by targeting Rac1.

  2024cites this paper
• The influences of ApoE isoforms on endothelial adherens junctions and actin cytoskeleton responding to mCRP

  2024cites this paper
• Balancing limited resources in actin networks competition

  2024cites this paper
• Calcineurin is an adaptor required for assembly of the TCR signaling complex

  2024cites this paper
• Focal adhesions contain three specialized actin nanoscale layers

  2024cites this paper
• Mitotic outcomes and errors in fibrous environments

  2023cites this paper
• A molecular basis of tumor metastasis and current approaches to decode targeted migration-promoting events in pediatric neuroblastoma.

  2023cites this paper
• Differentially expressed transcripts of Tetracapsuloides bryosalmonae (Cnidaria) between carrier and dead-end hosts involved in key biological processes: novel insights from a coupled approach of FACS and RNA sequencing

  2023cites this paper
• A New Monoterpene Alkaloid from Incarvillea sinensis with Migration Inhibitory Activity on Cancer Cell

  2023cites this paper
• Actin Bundles Dynamics and Architecture

  2023cites this paper
• Specialized actin nanoscale layers control focal adhesion turnover

  2023cites this paper
• VASP boosts protrusive activity of macroendocytic cups and drives phagosome rocketing after internalization.

  2022cites this paper
• ‘Merging of ventral fibers at adhesions drives the remodeling of cellular contractile systems in fibroblasts.’

  2022cites this paper
• Regeneration in calcareous sponge relies on ‘purse-string’ mechanism and the rearrangements of actin cytoskeleton

  2022cites this paper
• ZnR/GPR39 controls cell migration by orchestrating recruitment of KCC3 into protrusions, re-organization of actin and activation of MMP.

  2021cites this paper
• Role of actin cytoskeleton in the organization and function of ionotropic glutamate receptors

  2021cites this paper
• Transmigration across a Steady-State Blood–Brain Barrie Induces Activation of Circulating Dendritic Cells Partly Mediated by Actin Cytoskeletal Reorganization

  2021cites this paper
• Cannabis chemistry, post-harvest processing methods and secondary metabolite profiling: A review

  2021cites this paper
• Discrete mechanical model of lamellipodial actin network implements molecular clutch mechanism and generates arcs and microspikes

  2021cites this paper
• Calcium–cytoskeleton signaling–induced modification of plant development

  2021cites this paper
• The multiple roles of actin-binding proteins at invadopodia.

  2021cites this paper
• Actin polymerization downstream of integrins: signaling pathways and mechanotransduction.

  2020cites this paper
• Modulation of Actin Filament Dynamics by Inward Rectifying of Potassium Channel Kir2.1

  2020cites this paper
• Loss of Ena/VASP interferes with lamellipodium architecture, motility and integrin-dependent adhesion

  2020cites this paper
• Roles of the actin cytoskeleton in aging and age-associated diseases.

  2020cites this paper
• Fast super-resolution imaging technique and immediate early nanostructure capturing by a photo-convertible fluorescent protein.

  2020cites this paper
• A proof of concept study demonstrating that environmental levels of carbamazepine impair early stages of chick embryonic development.

  2019cites this paper
• Leptin induces cell migration and invasion in a FAK-Src-dependent manner in breast cancer cells

  2019cites this paper
• Self-assembly of magnetically-functionalized molecular motors and microtubules into active gels.

  2019cites this paper
• Spatial integration of mechanical forces by α-actinin establishes actin network symmetry

  2019cites this paper
• Extracellular Matrix in Regulation of Contractile System in Cardiomyocytes

  2019cites this paper
• Spatial integration of mechanical forces by alpha-actinin establishes actin network symmetry

  2019cites this paper
• Fabrication of Functional High Density Cell Sheets for Tissue Engineering Using Spin-Coated poly(N-isopropylacrylamide)Thin Films

  2019cites this paper
• Nanoparticle-based local translation reveals mRNA as a translation-coupled scaffold with anchoring function

  2018cites this paper
• Rasa3 controls turnover of endothelial cell adhesion and vascular lumen integrity by a Rap1-dependent mechanism

  2018cites this paper
• Role of endocytic proteins in mechanotransduction and impact on autosomal dominant centronuclear myopathy

  2018cites this paper
• Investigating adrenoceptor regulation of the astroglial glycogen reserve

  2017cites this paper
• Rho GTPases and actomyosin: Partners in regulating epithelial cell-cell junction structure and function.

  2017cites this paper
• Architecture and Connectivity Govern Actin Network Contractility.

  2016cites this paper
• Exploration par simulations numériques de l'auto-organisation du cytosquelette sous conditions géométriquement contrôlées. (Exploration by numerical simulations of cytoskeleton auto-organization)

  2015influential citation