Defining the earliest step of cardiovascular progenitor specification during embryonic stem cell differentiation

Mesp1, the earliest marker of cardiovascular development in vivo, is used to isolate and characterize multipotent cardiovascular progenitors during ESC differentiation.

• Publication year

  2011

• Venue

  Journal of Cell Biology

• Publication date

  2011-03-07

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1083/jcb.201007063PMID 21383076PMCID 3051813
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The Cellular Prion Protein Identifies Bipotential Cardiomyogenic Progenitors

  2010cited by this paper
• Developmental mechanisms, patterning and evolution.

  2010cited by this paper
• Early Chordate Origins of the Vertebrate Second Heart Field

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• Mesp1: A Key Regulator of Cardiovascular Lineage Commitment

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• A Regulatory Pathway Involving Notch1/β-Catenin/Isl1 Determines Cardiac Progenitor Cell Fate

  2009cited by this paper
• Distinct origins and genetic programs of head muscle satellite cells.

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• Human ISL1 heart progenitors generate diverse multipotent cardiovascular cell lineages

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• Mesp1 coordinately regulates cardiovascular fate restriction and epithelial-mesenchymal transition in differentiating ESCs.

  2008cited by this paper
• Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources

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• Development and tissue origins of the mammalian cranial base.

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• Reassessment of Isl1 and Nkx2-5 cardiac fate maps using a Gata4-based reporter of Cre activity.

  2008cited by this paper
• Cell lineage in mammalian craniofacial mesenchyme.

  2008cited by this paper
• MesP1 drives vertebrate cardiovascular differentiation through Dkk-1-mediated blockade of Wnt-signalling

  2008cited by this paper
• Mesp1 acts as a master regulator of multipotent cardiovascular progenitor specification.

  2008influential reference
• CXCR4+/FLK‐1+ Biomarkers Select a Cardiopoietic Lineage from Embryonic Stem Cells

  2008cited by this paper
• Lives of a heart cell: tracing the origins of cardiac progenitors.

  2008cited by this paper
• Differentiation of embryonic stem cells to clinically relevant populations: lessons from embryonic development.

  2008cited by this paper
• Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population

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• Islet 1 is expressed in distinct cardiovascular lineages, including pacemaker and coronary vascular cells.

  2007cited by this paper
• Specification of multipotential cardiovascular progenitor cells during embryonic stem cell differentiation and embryonic development.

  2007cited by this paper
• The amphibian second heart field: Xenopus islet-1 is required for cardiovascular development.

  2007cited by this paper
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  2007cited by this paper
• Multipotent flk-1+ cardiovascular progenitor cells give rise to the cardiomyocyte, endothelial, and vascular smooth muscle lineages.

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• Gene Regulatory Networks in the Evolution and Development of the Heart

  2006cited by this paper
• In Brief

  2006influential reference
• Gene Regulatory Networks in the Evolution and Development of the Heart

  2006cited by this paper
• Deletion of the selection cassette, but not cis-acting elements, in targeted Flk1-lacZ allele reveals Flk1 expression in multipotent mesodermal progenitors.

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• Identification of cardiac stem cells with FLK1, CD31 and VE-cadherin expression during embryonic stem cell differentiation

  2005cited by this paper
• The clonal origin of myocardial cells in different regions of the embryonic mouse heart.

  2004cited by this paper
• DAVID: Database for Annotation, Visualization, and Integrated Discovery

  2003cited by this paper
• A highly efficient recombineering-based method for generating conditional knockout mutations.

  2003cited by this paper
• Isl1 identifies a cardiac progenitor population that proliferates prior to differentiation and contributes a majority of cells to the heart.

  2003cited by this paper
• HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitors.

  2002cited by this paper
• Transcriptional regulation of Mesp1 and Mesp2 genes: differential usage of enhancers during development.

  2001cited by this paper
• MesP1 and MesP2 are essential for the development of cardiac mesoderm.

  2000cited by this paper
• Mesp1 expression is the earliest sign of cardiovascular development.

  2000cited by this paper
• MesP1 is expressed in the heart precursor cells and required for the formation of a single heart tube.

  1999cited by this paper
• PDGFRα Expression During Mouse Embryogenesis: Immunolocalization Analyzed by Whole-mount Immunohistostaining Using the Monoclonal Anti-mouse PDGFRα Antibody APA5

  1997cited by this paper
• PDGFR alpha expression during mouse embryogenesis: immunolocalization analyzed by whole-mount immunohistostaining using the monoclonal anti-mouse PDGFR alpha antibody APA5.

  1997cited by this paper
• MesP1: a novel basic helix-loop-helix protein expressed in the nascent mesodermal cells during mouse gastrulation.

  1996influential reference
• Functional domains of the Drosophila Engrailed protein.

  1993influential reference
• The FASEB Journal • Research Communication Identification of cardiac stem cells with FLK1, CD31,

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  2023cites this paper
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  2022cites this paper
• Cardiac stem cells: Current knowledge and future prospects

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• Cardiac specification during gastrulation - The Yellow Brick Road leading to Tinman.

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• Induced pluripotent stem cell-derived cardiomyocytes

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• The Future of Direct Cardiac Reprogramming: Any GMT Cocktail Variety?

  2020cites this paper
• Cardiac regeneration

  2020cites this paper
• Capturing Cardiogenesis in Gastruloids.

  2020influential citation
• Exploring the roles of MACIT and Multiplexin collagens in stem cells and cancer.

  2020cites this paper
• Comprehensive Overview of Non-coding RNAs in Cardiac Development.

  2020cites this paper
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  2020cites this paper
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  2019cites this paper
• Stem Cells Heterogeneity in Different Organs

  2019cites this paper
• Heterogeneity of Adult Cardiac Stem Cells.

  2019cites this paper
• Cardiac Progenitor Cells from Stem Cells: Learning from Genetics and Biomaterials

  2019influential citation
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  2019cites this paper
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