Rad54p Is a Chromatin Remodeling Enzyme Required for Heteroduplex DNA Joint Formation with Chromatin*

In eukaryotic cells, the repair of DNA double-strand breaks by homologous recombination requires a RecA-like recombinase, Rad51p, and a Swi2p/Snf2p-like ATPase, Rad54p. Here we find that yeast Rad51p and Rad54p support robust homologous pairing between single-stranded DNA and a chromatin donor. In contrast, bacterial RecA is incapable of catalyzing homologous pairing with a chromatin donor. We also show that Rad54p possesses many of the biochemical properties of bona fideATP-dependent chromatin-remodeling enzymes, such as ySWI/SNF. Rad54p can enhance the accessibility of DNA within nucleosomal arrays, but it does not seem to disrupt nucleosome positioning. Taken together, our results indicate that Rad54p is a chromatin-remodeling enzyme that promotes homologous DNA pairing events within the context of chromatin.

• Publication year

  2003

• Venue

  Journal of Biological Chemistry

• Publication date

  2003-03-14

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1074/JBC.M211545200PMID 12514177
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• IL-13受体α2降低血吸虫病肉芽肿的炎症反应并延长宿主存活时间[英]/Mentink-Kane MM,Cheever AW，Thompson RW，et al//Proc Natl Acad Sci U S A

  2005cited by this paper
• Chromatin remodeling by RSC involves ATP-dependent DNA translocation.

  2002cited by this paper
• When repair meets chromatin

  2002cited by this paper
• Functional Cross-talk among Rad51, Rad54, and Replication Protein A in Heteroduplex DNA Joint Formation*

  2002cited by this paper
• Strand pairing by Rad54 and Rad51 is enhanced by chromatin.

  2002cited by this paper
• A unified view of the DNA-damage checkpoint.

  2002cited by this paper
• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• Rad54 protein stimulates heteroduplex DNA formation in the synaptic phase of DNA strand exchange via specific interactions with the presynaptic Rad51 nucleoprotein filament.

  2001cited by this paper
• The architecture of the human Rad54–DNA complex provides evidence for protein translocation along DNA

  2001cited by this paper
• Human DNA Repair Genes

  2001cited by this paper
• DNA double-strand breaks: signaling, repair and the cancer connection

  2001cited by this paper
• Recombination: homologous recombination branches out.

  2001cited by this paper
• Rad54 protein stimulates the postsynaptic phase of Rad51 protein-mediated DNA strand exchange

  2001cited by this paper
• Tailed duplex DNA is the preferred substrate for Rad51 protein‐mediated homologous pairing

  2000cited by this paper
• Measuring motion on DNA by the type I restriction endonuclease EcoR124I using triplex displacement

  2000cited by this paper
• Superhelicity-driven homologous DNA pairing by yeast recombination factors Rad51 and Rad54.

  2000cited by this paper
• Generation of superhelical torsion by ATP-dependent chromatin remodeling activities.

  2000cited by this paper
• SWI-SNF-Mediated Nucleosome Remodeling: Role of Histone Octamer Mobility in the Persistence of the Remodeled State

  2000cited by this paper
• Promoter targeting and chromatin remodeling by the SWI/SNF complex.

  2000cited by this paper
• Demonstration of unidirectional single-stranded DNA translocation by PcrA helicase: measurement of step size and translocation speed.

  2000cited by this paper
• ATP-Dependent histone octamer mobilization and histone deacetylation mediated by the Mi-2 chromatin remodeling complex.

  2000cited by this paper
• Multiple Pathways of Recombination Induced by Double-Strand Breaks in Saccharomyces cerevisiae

  1999cited by this paper
• Nucleosome movement by CHRAC and ISWI without disruption or trans-displacement of the histone octamer.

  1999cited by this paper
• Crystal structures of complexes of PcrA DNA helicase with a DNA substrate indicate an inchworm mechanism.

  1999cited by this paper
• ATP-dependent histone octamer sliding mediated by the chromatin remodeling complex NURF.

  1999cited by this paper
• Purification and biochemical properties of yeast SWI/SNF complex.

  1999cited by this paper
• Yeast Rad54 Promotes Rad51-dependent Homologous DNA Pairing via ATP Hydrolysis-driven Change in DNA Double Helix Conformation*

  1999cited by this paper
• Nucleosome mobilization catalysed by the yeast SWI/SNF complex

  1999cited by this paper
• Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins

  1998cited by this paper
• Molecular mechanisms of DNA double strand break repair.

  1998cited by this paper
• Recombinational repair in yeast: functional interactions between Rad51 and Rad54 proteins

  1997cited by this paper
• Catalytic activity of the yeast SWI/SNF complex on reconstituted nucleosome arrays

  1997cited by this paper
• Direct Association between the Yeast Rad51 and Rad54 Recombination Proteins*

  1996cited by this paper
• Evolution of the SNF2 family of proteins: subfamilies with distinct sequences and functions.

  1995cited by this paper
• Homogeneous reconstituted oligonucleosomes, evidence for salt-dependent folding in the absence of histone H1.

  1989cited by this paper
• Enzyme-catalyzed DNA unwinding: studies on Escherichia coli rep protein.

  1979cited by this paper
• DNA folding by histones: the kinetics of chromatin core particle reassembly and the interaction of nucleosomes with histones.

  1979cited by this paper

• Structural basis for Rad54- and Hed1-mediated regulation of Rad51 during the transition from mitotic to meiotic recombination

  2025cites this paper
• All who wander are not lost: the search for homology during homologous recombination

  2024cites this paper
• The translocation activity of Rad54 reduces crossover outcomes during homologous recombination

  2024cites this paper
• TLK1-mediated RAD54 phosphorylation spatio-temporally regulates Homologous Recombination Repair

  2023cites this paper
• Perspectives on ATP-dependent chromatin remodeling.

  2023cites this paper
• Disparate requirements for RAD54L in replication fork reversal

  2023cites this paper
• RAD51AP1 and RAD54L Can Underpin Two Distinct RAD51-Dependent Routes of DNA Damage Repair via Homologous Recombination

  2022cites this paper
• Disparate roles for C. elegans DNA translocase paralogs RAD-54.L and RAD-54.B in meiotic prophase germ cells

  2022cites this paper
• Drosophila SUMM4 complex couples insulator function and DNA replication control

  2022cites this paper
• Rdh54 stabilizes Rad51 at displacement loop intermediates to regulate genetic exchange between chromosomes

  2022cites this paper
• Drosophila SUMM4 complex couples insulator function and DNA replication timing control

  2021cites this paper
• RAD51AP1 and RAD54 underpin two distinct RAD51-dependent routes of DNA damage repair via homologous recombination

  2021cites this paper
• Repression of a large number of genes requires interplay between homologous recombination and HIRA

  2021cites this paper
• Discrete roles for Rad54 and Rdh54 during homologous recombination.

  2021cites this paper
• Enhancement of homology-directed repair with chromatin donor templates in cells

  2020cites this paper
• Rad54 Drives ATP Hydrolysis-Dependent DNA Sequence Alignment during Homologous Recombination.

  2020cites this paper
• RAD51AP1 mediates RAD51 activity through nucleosome interaction

  2020cites this paper
• NUCKS1 promotes RAD54 activity in homologous recombination DNA repair

  2020cites this paper
• ATRX tames repetitive DNA within heterochromatin to promote normal brain development and regulate oncogenesis

  2019cites this paper
• Regulation of Hed1 and Rad54 binding during maturation of the meiosis‐specific presynaptic complex

  2018cites this paper
• Function and Interactions of ERCC1-XPF in DNA Damage Response

  2018cites this paper
• RAD54 N-terminal domain is a DNA sensor that couples ATP hydrolysis with branch migration of Holliday junctions

  2018cites this paper
• Homologous recombination and the repair of DNA double-strand breaks

  2018influential citation
• BIME2, a novel gene required for interhomolog meiotic recombination in the protist model organism Tetrahymena

  2017cites this paper
• Chromatin architecture may dictate the target site for DMC1, but not for RAD51, during homologous pairing

  2016cites this paper
• Rad51 and Rad54 promote noncrossover recombination between centromere repeats on the same chromatid to prevent isochromosome formation

  2016cites this paper
• Mechanism of Homologous Recombination

  2016cites this paper
• Homologous recombinaton within a chromatin environment

  2016cites this paper
• Relaxed Chromatin Formation and Weak Suppression of Homologous Pairing by the Testis-Specific Linker Histone H1T.

  2016cites this paper
• Yeast HMO1: Linker Histone Reinvented

  2016cites this paper
• In Silico Strategies to Modulate DNA Damage Response

  2015cites this paper
• Mating-type Gene Switching in Saccharomyces cerevisiae

  2015cites this paper
• Nap1 stimulates homologous recombination by RAD51 and RAD54 in higher-ordered chromatin containing histone H1

  2014cites this paper
• Mechanisms and Regulation of Mitotic Recombination in Saccharomyces cerevisiae

  2014cites this paper
• Mechanisms and principles of homology search during recombination

  2014cites this paper
• Characterization of the Interaction between the Saccharomyces cerevisiae Rad51 Recombinase and the DNA Translocase Rdh54*

  2013cites this paper
• DNA Helicases and DNA Motor Proteins

  2013cites this paper
• The PCNA Interaction Protein Box Sequence in Rad54 Is an Integral Part of Its ATPase Domain and Is Required for Efficient DNA Repair and Recombination

  2013cites this paper
• RAD51 Is a Key Protein of DNA Repair and Homologous Recombination in Humans

  2013cites this paper
• Analysis of the Activities of RAD54, a SWI2/SNF2 Protein, Using a Specific Small-molecule Inhibitor*

  2013influential citation
• FOCuS: 50 YEARS OF DNA REPAiR: THE YAlE SYMPOSiuM

  2013cites this paper
• ATP-Dependent Chromatin Remodeling.

  2013cites this paper
• Homologous recombination in eukaryotes.

  2012cites this paper
• Regulation of Nucleosome Dynamics: Mechanisms for Chromatin Accessibility and Metabolism

  2012cites this paper
• Loss of Atrx Sensitizes Cells to DNA Damaging Agents through p53-Mediated Death Pathways

  2012cites this paper
• MECHANISM AND REGULATION OF CHROMATIN REMODELER, RSC by

  2012cites this paper
• Mating-Type Genes and MAT Switching in Saccharomyces cerevisiae

  2012cites this paper
• Compromised genomic integrity impedes muscle growth after Atrx inactivation.

  2012cites this paper
• Eukaryotic Homologous Recombination Repair: A Dynamic Cast of Characters

  2012cites this paper
• SURVEY AND SUMMARY Homologous recombination and its regulation

  2012cites this paper
• Chromatin and the genome integrity network

  2012cites this paper
• Homologous recombination and its regulation

  2012cites this paper
• Over-expression of RAD51 or RAD54 but not RAD51/4 enhances extra-chromosomal homologous recombination in the human sarcoma (HT-1080) cell line.

  2011cites this paper
• Insights into Regulation of Human RAD51 Nucleoprotein Filament Activity During Homologous Recombination

  2011cites this paper
• Functions of the Snf2/Swi2 family Rad54 motor protein in homologous recombination.

  2011cites this paper
• Eukaryote DNA replication and recombination : an intimate association

  2011cites this paper
• Interplay and Quality Control of DNA Damage Repair Mechanisms

  2011cites this paper
• Genome-destabilizing and mutagenic effects of break-induced replication in Saccharomyces cerevisiae

  2011cites this paper
• ATP-dependent and independent functions of Rad54 in genome maintenance

  2011cites this paper
• Reconstitution of DNA repair synthesis in vitro and the role of polymerase and helicase activities

  2011cites this paper
• Mécanismes impliqués dans la formation des anomalies chromosomiques lors de la meiose en absence de brca2 chez la plante arabidopsis thaliana

  2011cites this paper
• One small step for Mot1; one giant leap for other Swi2/Snf2 enzymes?

  2011cites this paper
• RSC Facilitates Rad59-Dependent Homologous Recombination between Sister Chromatids by Promoting Cohesin Loading at DNA Double-Strand Breaks

  2011cites this paper
• The response of mammalian cells to UV-light reveals Rad54-dependent and independent pathways of homologous recombination.

  2011cites this paper
• Functional significance of mutations in the Snf2 domain of ATRX.

  2011cites this paper
• Rad54, the motor of homologous recombination.

  2010cites this paper
• Single-molecule imaging brings Rad51 nucleoprotein filaments into focus.

  2010cites this paper
• Time to Bloom

  2010cites this paper
• Author ' s personal copy Single-molecule imaging brings Rad 51 nucleoprotein filaments into focus

  2010cites this paper
• Analysis of the relationships between ATM and the Rad54 paralogs involved in homologous recombination repair.

  2009cites this paper
• Transcriptional Suppression by Transient Recruitment of ARIP4 to Sumoylated nuclear receptor Ad4BP/SF-1.

  2009cites this paper
• ATP-dependent Remodelling of Linker Histone-Containing Nucleosomal Fibres

  2009cites this paper
• Recombinational Repair of a Chromosomal DNA Double Strand Break: A Dissertation

  2009influential citation
• Functional interactions of meiotic recombination factors Rdh54 and Dmc1.

  2009cites this paper
• Localization of recombination proteins and Srs2 reveals anti-recombinase function in vivo

  2009cites this paper
• Recombinational repair within heterochromatin requires ATP-dependent chromatin remodeling.

  2009cites this paper
• Influence de l'histone de liaison sur la dynamique de fibres de chromatine individuelles

  2009cites this paper
• A DNA translocating Snf2 molecular motor: S. cerevisiae Rdh54 displays processive translocation and can extrude DNA loops

  2009cites this paper
• FANCJ Uses Its Motor ATPase to Destabilize Protein-DNA Complexes, Unwind Triplexes, and Inhibit RAD51 Strand Exchange*

  2009cites this paper
• NO sparks off chromatin: tales of a multifaceted epigenetic regulator.

  2009cites this paper
• Studies on the mechanism of nucleosome remodeling by RSC and SWI/SNF

  2009cites this paper
• An archaeal Rad54 protein remodels DNA and stimulates DNA strand exchange by RadA

  2009cites this paper
• ATP-dependent Chromatin Remodeling by the Saccharomyces cerevisiae Homologous Recombination Factor Rdh54*

  2008cites this paper
• ATP-dependent chromatin remodeling shapes the DNA replication landscape

  2008cites this paper
• Biochemistry of Meiotic Recombination: Formation, Processing, and Resolution of Recombination Intermediates.

  2008cites this paper
• Large inverted repeats in the vicinity of a single double-strand break strongly affect repair in yeast diploids lacking Rad51.

  2008cites this paper
• Filament formation and robust strand exchange activities of the rice DMC1A and DMC1B proteins

  2008cites this paper
• A Rad51 presynaptic filament is sufficient to capture nucleosomal homology during recombinational repair of a DNA double-strand break.

  2008cites this paper
• Rad51 Polymerization Reveals a New Chromatin Remodeling Mechanism

  2008cites this paper
• Biochemical analysis of the N-terminal domain of human RAD54B

  2008cites this paper
• FANCM of the Fanconi anemia core complex is required for both monoubiquitination and DNA repair.

  2008cites this paper
• THE ROLE OF CHROMATIN MODIFICATIONS IN THE REGULATION OF DNA REPLICATION IN SACCHAROMYCES CEREVISIAE

  2008cites this paper
• HARP Is an ATP-Driven Annealing Helicase

  2008cites this paper
• Rad51 Protein Stimulates the Branch Migration Activity of Rad54 Protein*

  2008cites this paper
• The Cell Biology of Rad54: Implications for homologous recombination

  2008cites this paper
• Overcoming obstacles to nonhomologous end joining repair of chromosome double strand breaks

  2008cites this paper
• Homologous recombination in DNA repair and DNA damage tolerance

  2008cites this paper
• Androgen receptor-dependent and independent functions of ARIP4

  2007cites this paper
• Single Molecule Imaging of Tid1/Rdh54, a Rad54 Homolog That Translocates on Duplex DNA and Can Disrupt Joint Molecules*

  2007cites this paper
• Rad54 oligomers translocate and cross-bridge double-stranded DNA to stimulate synapsis.

  2007cites this paper