Probing molecular motion by NMR.

Recently developed solution NMR methods for measuring 2H, 13C, and 15N spin relaxation, coupled with biosynthetic isotopic enrichment, permit the characterization of backbone and sidechain dynamical properties of proteins on picosecond/nanosecond and microsecond/millisecond timescales. Theoretical interpretations of the relaxation data provide insights into the biophysical and functional properties of proteins.

• Publication year

  1997

• Venue

  Current Opinion in Structural Biology

• Publication date

  1997-10-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/S0959-440X(97)80085-1PMID 9345634
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Module-module interactions in the cell binding region of fibronectin: stability, flexibility and specificity.

  1997cited by this paper
• Changes in the NMR-derived motional parameters of the insulin receptor substrate 1 phosphotyrosine binding domain upon binding to an interleukin 4 receptor phosphopeptide.

  1997cited by this paper
• Backbone dynamics of the major coat protein of bacteriophage M13 in detergent micelles by 15N nuclear magnetic resonance relaxation measurements using the model-free approach and reduced spectral density mapping.

  1997cited by this paper
• The main-chain dynamics of the dynamin pleckstrin homology (PH) domain in solution: analysis of 15N relaxation with monomer/dimer equilibration.

  1997cited by this paper
• Systematic Errors Associated with the CPMG Pulse Sequence and Their Effect on Motional Analysis of Biomolecules

  1997cited by this paper
• High resolution solution structure of ribosomal protein L11-C76, a helical protein with a flexible loop that becomes structured upon binding to RNA

  1997cited by this paper
• Backbone and side chain dynamics of lac repressor headpiece (1-56) and its complex with DNA.

  1997cited by this paper
• Heteronuclear NMR studies of E. coli translation initiation factor IF3. Evidence that the inter-domain region is disordered in solution.

  1997cited by this paper
• A Protocol for the Interpretation of Side-Chain Dynamics Based on NMR Relaxation: Application to Phenylalanines in Antamanide

  1997cited by this paper
• Characterization of the backbone dynamics of folded and denatured states of an SH3 domain.

  1997cited by this paper
• Defining long range order in NMR structure determination from the dependence of heteronuclear relaxation times on rotational diffusion anisotropy

  1997cited by this paper
• Ligand binding alters the backbone mobility of intestinal fatty acid-binding protein as monitored by 15N NMR relaxation and 1H exchange.

  1997cited by this paper
• Deuterium Relaxation in a Uniformly 15N-Labeled Homeodomain and Its DNA Complex1

  1997cited by this paper
• Study of protein dynamics in solution by measurement of 13Cα-13CO NOE and 13CO longitudinal relaxation

  1996cited by this paper
• Accretion of structure in staphylococcal nuclease: an 15N NMR relaxation study.

  1996cited by this paper
• Structure and dynamics of bacteriophage IKe major coat protein in MPG micelles by solution NMR.

  1996cited by this paper
• Dynamic studies of a fibronectin type I module pair at three frequencies: Anisotropic modelling and direct determination of conformational exchange

  1996cited by this paper
• Protein Backbone Dynamics and 15N Chemical Shift Anisotropy from Quantitative Measurement of Relaxation Interference Effects

  1996cited by this paper
• Dynamics of ribonuclease H: temperature dependence of motions on multiple time scales.

  1996cited by this paper
• Three-dimensional solution structure and backbone dynamics of a variant of human interleukin-3.

  1996cited by this paper
• Correlation between dynamics and high affinity binding in an SH2 domain interaction.

  1996cited by this paper
• Anisotropic rotational diffusion of perdeuterated HIV protease from 15N NMR relaxation measurements at two magnetic fields

  1996cited by this paper
• Backbone dynamics of a domain of protein L which binds to immunoglobulin light chains.

  1996cited by this paper
• Measurement of 13Cα-13CO cross-relaxation rates in 15N-/13C-labelled proteins

  1996cited by this paper
• Main-chain dynamics of a partially folded protein: 15N NMR relaxation measurements of hen egg white lysozyme denatured in trifluoroethanol.

  1996cited by this paper
• Internal dynamics of human ubiquitin revealed by 13C-relaxation studies of randomly fractionally labeled protein.

  1996cited by this paper
• Backbone dynamics of the 269-residue protease Savinase determined from 15N-NMR relaxation measurements.

  1996cited by this paper
• Phosphotransfer and CheY-binding domains of the histidine autokinase CheA are joined by a flexible linker.

  1996cited by this paper
• 15N NMR relaxation studies of free and inhibitor-bound 4-oxalocrotonate tautomerase: backbone dynamics and entropy changes of an enzyme upon inhibitor binding.

  1996cited by this paper
• Contributions to conformational entropy arising from bond vector fluctuations measured from NMR-derived order parameters: application to protein folding.

  1996cited by this paper
• Internal mobility in the partially folded DNA binding and dimerization domains of GAL4: NMR analysis of the N-H spectral density functions.

  1996cited by this paper
• Backbone dynamics of the C-terminal domain of Escherichia coli topoisomerase I in the absence and presence of single-stranded DNA.

  1996cited by this paper
• Insights into the local residual entropy of proteins provided by NMR relaxation

  1996cited by this paper
• Dynamical Mapping of E. coli Thioredoxin via 13C NMR Relaxation Analysis

  1996cited by this paper
• Monitoring Macromolecular Motions on Microsecond to Millisecond Time Scales by R1ρ−R1 Constant Relaxation Time NMR Spectroscopy

  1996cited by this paper
• CommunicationThe Effects of Cross Correlation and Cross Relaxation on the Measurement of DeuteriumT1andT1ρRelaxation Times in13CH2D Spin Systems

  1996cited by this paper
• Frequency spectrum of NH bonds in eglin c from spectral density mapping at multiple fields.

  1995cited by this paper
• Flexibility and function in HIV-1 protease

  1995cited by this paper
• Backbone dynamics of trp repressor studied by 15N NMR relaxation.

  1995cited by this paper
• Dynamics of the dihydrofolate reductase-folate complex: catalytic sites and regions known to undergo conformational change exhibit diverse dynamical features.

  1995cited by this paper
• Structural and dynamic characterization of the phosphotyrosine binding region of a Src homology 2 domain--phosphopeptide complex by NMR relaxation, proton exchange, and chemical shift approaches.

  1995cited by this paper
• Backbone dynamics of the A-domain of HMG1 as studied by 15N NMR spectroscopy.

  1995cited by this paper
• Structural determinants of protein dynamics: analysis of 15N NMR relaxation measurements for main-chain and side-chain nuclei of hen egg white lysozyme.

  1995cited by this paper
• Rotational diffusion anisotropy of human ubiquitin from 15N NMR relaxation

  1995cited by this paper
• Structural and dynamic characterization of the urea denatured state of the immunoglobulin binding domain of streptococcal protein G by multidimensional heteronuclear NMR spectroscopy

  1995cited by this paper
• Rotational dynamics of calcium-free calmodulin studied by 15N-NMR relaxation measurements.

  1995cited by this paper
• Spectral density function mapping using 15N relaxation data exclusively

  1995cited by this paper
• Comparison of the backbone dynamics of a folded and an unfolded SH3 domain existing in equilibrium in aqueous buffer.

  1995cited by this paper
• Measurement of 2H T1 and T1.rho. Relaxation Times in Uniformly 13C-Labeled and Fractionally 2H-Labeled Proteins in Solution

  1995cited by this paper
• Backbone dynamics of Escherichia coli ribonuclease HI: correlations with structure and function in an active enzyme.

  1995cited by this paper
• Long-range motional restrictions in a multidomain zinc-finger protein from anisotropic tumbling.

  1995cited by this paper
• Quasi-Spectral-Density Function Analysis for Nitrogen-15 Nuclei in Proteins

  1995cited by this paper
• FLEXIBILITY OF AN ARGININE SIDE CHAIN AT A DNA-PROTEIN INTERFACE

  1995cited by this paper
• Measurement of 15N Relaxation Data from the Side Chains of Asparagine and Glutamine Residues in Proteins

  1995cited by this paper
• A test of the model-free formulas. Effects of anisotropic rotational diffusion and dimerization.

  1994cited by this paper
• NMR Order Parameters of Biomolecules: A New Analytical Representation and Application to the Gaussian Axial Fluctuation Model

  1994cited by this paper
• Comparison of backbone and tryptophan side-chain dynamics of reduced and oxidized Escherichia coli thioredoxin using 15N NMR relaxation measurements.

  1993cited by this paper
• NMR order parameters and free energy: an analytical approach and its application to cooperative calcium(2+) binding by calbindin D9k

  1993cited by this paper
• Model-free approach to the interpretation of nuclear magnetic resonance relaxation in macromolecules. 1. Theory and range of validity

  1982cited by this paper

• Fitting Force Field parameters to NMR Relaxation Data

  2023cites this paper
• The Prenucleation Equilibrium of the Parathyroid Hormone Determines the Critical Aggregation Concentration and Amyloid Fibril Nucleation.

  2023cites this paper
• Solution structure of the N-terminal extension domain of a Schistosoma japonicum asparaginyl-tRNA synthetase

  2023cites this paper
• A Highly Ordered Nitroxide Side Chain for Distance Mapping and Monitoring Slow Structural Fluctuations in Proteins

  2023cites this paper
• Determination of inclusion geometry of cyclodextrin host-guest complexes: Applicability of 1D selective NMR methods

  2022cites this paper
• NMR Reveals Functionally Relevant Thermally Induced Structural Changes within the Native Ensemble of G-CSF

  2022cites this paper
• NMR spectroscopy, excited states and relevance to problems in cell biology - transient pre-nucleation tetramerization of huntingtin and insights into Huntington's disease.

  2022cites this paper
• Polyanions Cause Protein Destabilization Similar to That in Live Cells

  2021cites this paper
• Protein structure prediction using residue-resolved protection factors from hydrogen-deuterium exchange NMR.

  2021cites this paper
• Protein Structure Prediction from NMR Hydrogen-Deuterium Exchange Data.

  2021cites this paper
• Fitting side-chain NMR relaxation data using molecular simulations

  2020cites this paper
• Impact of the Hereditary P301L Mutation on the Correlated Conformational Dynamics of Human Tau Protein Revealed by the Paramagnetic Relaxation Enhancement NMR Experiments

  2020cites this paper
• Diffusive protein interactions in human versus bacterial cells

  2020cites this paper
• Ultrasensitive Change in Nucleosome Binding by Multiple Phosphorylations to the Intrinsically Disordered Region of the Histone Chaperone FACT.

  2020cites this paper
• Quo Vadis Biomolecular NMR Spectroscopy?

  2019cites this paper
• Rotational and Translational Diffusion of Proteins as a Function of Concentration

  2019cites this paper
• Side-Chain Dynamics of the Trifluoroacetone Cysteine Derivative Characterized by 19F NMR Relaxation and Molecular Dynamics Simulations.

  2019cites this paper
• Characterizing Protein Dynamics with NMR R 1ρ Relaxation Experiments.

  2018cites this paper
• Global Structural Flexibility of Metalloproteins Regulates Reactivity of Transition Metal Ion in the Protein Core: An Experimental Study Using Thiol-subtilisin as a Model Protein.

  2018cites this paper
• Protein NMR Studies of Substrate Binding to Human Blood Group A and B Glycosyltransferases

  2017cites this paper
• Molecular modeling and molecular dynamic simulation of the effects of variants in the TGFBR2 kinase domain as a paradigm for interpretation of variants obtained by next generation sequencing

  2017cites this paper
• NMR Analysis of Amide Hydrogen Exchange Rates in a Pentapeptide-Repeat Protein from A. thaliana.

  2017cites this paper
• Solution NMR studies of HIV-1 reverse transcriptase

  2017cites this paper
• Iterative cluster-NMA ( icNMA ) : A tool for generating conformational transitions in proteins

  2017cites this paper
• XAS Spectroscopy: Related Techniques and Combination with Other Spectroscopic and Scattering Methods

  2016cites this paper
• Modern Analysis of Protein Folding by Differential Scanning Calorimetry.

  2016cites this paper
• Independent Metrics for Protein Backbone and Side-Chain Flexibility: Time Scales and Effects of Ligand Binding.

  2015cites this paper
• Molecular dynamics in the hairpin ribozyme: Calculational and experimental aspects

  2015cites this paper
• Urea Dependent (15)N NMR-Relaxation Studies on PfP2 Multimers Reveal that the C-Terminal Behaves like an Independent Intrinsically Disordered Peptide.

  2015cites this paper
• Protein dynamics: from rattling in a cage to structural relaxation.

  2015cites this paper
• Allosteric Breakage of the Hydrogen Bond within the Dual-Histidine Motif in the Active Site of Human Pin1 PPIase.

  2015cites this paper
• Dynamics of small, folded proteins

  2014cites this paper
• Dynamic Pictures of Proteins by NMR

  2014cites this paper
• Sampling of Organic Solutes in Aqueous and Heterogeneous Environments Using Oscillating Excess Chemical Potentials in Grand Canonical-like Monte Carlo-Molecular Dynamics Simulations

  2014cites this paper
• Conformational propensities and dynamics of a βγ-crystallin, an intrinsically disordered protein.

  2014cites this paper
• An unusual mode of galactose recognition by a family 32 carbohydrate-binding module.

  2014cites this paper
• Structural and biochemical studies of Sporosarcina pasteurii UreE: a nickel-chaperone involved in the urease activation process

  2014cites this paper
• The p66 immature precursor of HIV‐1 reverse transcriptase

  2014cites this paper
• The number and location of EF hand motifs dictates the calcium dependence of polycystin‐2 function

  2014cites this paper
• Vibrational Echo Chemical Exchange Spectroscopy

  2013cites this paper
• Crystallographic and NMR evidence for flexibility in oligosaccharyltransferases and its catalytic significance.

  2013cites this paper
• Small pH and salt variations radically alter the thermal stability of metal-binding domains in the copper transporter, Wilson disease protein.

  2013cites this paper
• NMR RELAXATION METHODS TO DETECT PROTEIN DYNAMICS: EVALUATION OF ACCURACY, IMPROVEMENT OF THE METHODOLOGY, AND ITS APPLICATION

  2013influential citation
• Redox-sensitive structural change in the A-domain of HMGB1 and its implication for the binding to cisplatin modified DNA.

  2013cites this paper
• Allosteric activation of the Par-6 PDZ via a partial unfolding transition.

  2013cites this paper
• Redox-sensitive structural change in the A-domain of HMGB1 and its implication for the binding to cisplatin modified DNA.

  2013cites this paper
• Nuclear Magnetic Resonance Approaches in the Study of 2-Oxo Acid Dehydrogenase Multienzyme Complexes—A Literature Review

  2013cites this paper
• A compact high-speed mechanical sample shuttle for field-dependent high-resolution solution NMR.

  2012cites this paper
• Multi-Timescale Conformational Dynamics of the SH3 Domain of CD2-Associated Protein using NMR Spectroscopy and Accelerated Molecular Dynamics

  2012cites this paper
• NMR Insights into Folding and Self-Association of Plasmodium falciparum P2

  2012cites this paper
• Residual structure and dynamics in DMSO-d6 denatured dynein light chain protein.

  2012cites this paper
• Crystallization from Solutions Containing Multiple Conformers. 1. Modeling of Crystal Growth and Supersaturation

  2012cites this paper
• High-resolution NMR field-cycling device for full-range relaxation and structural studies of biopolymers on a shared commercial instrument

  2012cites this paper
• Effective Kinetic Methods and Tools in Investigating the Mechanism of Action of Specific Hydrolases

  2012cites this paper
• Solution-state nuclear magnetic resonance spectroscopy and protein folding.

  2011cites this paper
• Structure and function of the cardiac stress response protein MS1

  2011cites this paper
• Overview on the use of NMR to examine protein structure.

  2011cites this paper
• Copper Transporters and Chaperones

  2011cites this paper
• NMR insights into the core of GED assembly by H/D exchange coupled with DMSO dissociation and analysis of the denatured state.

  2011cites this paper
• Structural characterization of intramolecular Hg(2+) transfer between flexibly linked domains of mercuric ion reductase.

  2011cites this paper
• Probing protein dynamics by nuclear magnetic resonance.

  2011cites this paper
• Reduced fluorescence lifetime heterogeneity of 5-fluorotryptophan in comparison to tryptophan in proteins: implication for resonance energy transfer experiments.

  2011cites this paper
• Extraordinary μs-ms backbone dynamics in Arabidopsis thaliana peroxiredoxin Q.

  2011cites this paper
• Modeling loop entropy.

  2011cites this paper
• Structure of the EF-hand domain of polycystin-2 suggests a mechanism for Ca2+-dependent regulation of polycystin-2 channel activity

  2010cites this paper
• Resolving Conformational and Rotameric Exchange in Spin-Labeled Proteins Using Saturation Recovery EPR

  2010cites this paper
• Suppressed or recovered intensities analysis in site-directed (13)C NMR: assessment of low-frequency fluctuations in bacteriorhodopsin and D85N mutants revisited.

  2010cites this paper
• Chemical shift tensor - the heart of NMR: Insights into biological aspects of proteins.

  2010cites this paper
• Structural Basis for Capping Protein Sequestration by Myotrophin (V-1)*

  2010cites this paper
• The cold denatured state of the C-terminal domain of protein L9 is compact and contains both native and non-native structure.

  2010cites this paper
• SPARTA+: a modest improvement in empirical NMR chemical shift prediction by means of an artificial neural network

  2010cites this paper
• Mapping NMR chemical shift anisotropy parameters of backbone nuclei onto secondary structure elements in proteins

  2010cites this paper
• Interpretation of biomolecular NMR spin relaxation parameters.

  2010cites this paper
• Melting of a beta-hairpin peptide using isotope-edited 2D IR spectroscopy and simulations.

  2010cites this paper
• Direct measurements of protein backbone 15N spin relaxation rates from peak line-width using a fully-relaxed Accordion 3D HNCO experiment.

  2009cites this paper
• Investigation of the structure and function of glutamate receptor GluR2 using solution NMR spectroscopy

  2009cites this paper
• Automated NMR Assignment and Protein Structure Determination using Sparse Dipolar Coupling Constraints.

  2009cites this paper
• Practical aspects of 15N CPMG transverse relaxation experiments for proteins in solution

  2009cites this paper
• Frameworks for understanding long-range intra-protein communication.

  2009cites this paper
• Dynamics of a truncated prion protein, PrP(113–231), from 15N NMR relaxation: Order parameters calculated and slow conformational fluctuations localized to a distinct region

  2009cites this paper
• Author ' s personal copy Automated NMR assignment and protein structure determination using sparse dipolar coupling constraints

  2009cites this paper
• Selective characterization of microsecond motions in proteins by NMR relaxation.

  2009cites this paper
• Physical methods and molecular biology

  2009cites this paper
• Translational, rotational and internal dynamics of amyloid beta-peptides (Abeta40 and Abeta42) from molecular dynamics simulations.

  2009cites this paper
• Chapter 3 Applications of the NOE in Molecular Biology

  2009cites this paper
• Charge neutralization and collapse of the C‐terminal tail of alpha‐synuclein at low pH

  2009cites this paper
• Deuterium spin probes of backbone order in proteins: 2H NMR relaxation study of deuterated carbon alpha sites.

  2009cites this paper
• Hierarchy in guanidine unfolding of DLC8 dimer: regulatory functional implications.

  2009cites this paper
• Osmolyte perturbation reveals conformational equilibria in spin‐labeled proteins

  2009cites this paper
• Untersuchung von p38α MAP-Kinase/Inhibitor-Komplexen in Lösung mittels Kernresonanzspektroskopie

  2008cites this paper
• Backbone dynamics of the 18.5 kDa isoform of myelin basic protein reveals transient alpha-helices and a calmodulin-binding site.

  2008cites this paper
• Structure, dynamics, and selectivity of the sodium channel blocker mu-conotoxin SIIIA.

  2008cites this paper
• pH dependent unfolding characteristics of DLC8 dimer: Residue level details from NMR.

  2008cites this paper
• Structural characterization of proteins involved in cellular signaling and trafficking

  2008cites this paper
• Characterizing Protein / ligand Binding by DsC

  2008cites this paper
• 15N NMR relaxation studies of Y14F mutant of ketosteroid isomerase: the influence of mutation on backbone mobility.

  2008cites this paper
• Direct observation of fast protein conformational switching

  2008cites this paper
• Quantifying diffusion in mucosal systems by pulsed-gradient spin-echo NMR.

  2008cites this paper
• Dynamics of the Hck‐SH3 domain: Comparison of experiment with multiple molecular dynamics simulations

  2008cites this paper
• Dynamics of backbone conformational heterogeneity in Bacillus subtilis ribonuclease P protein.

  2007cites this paper