Calcified decellularized arterial scaffolds impact vascular smooth muscle cell transformation via downregulating α-SMA expression and upregulating OPN expression.

The underlying mechanisms of arterial remodeling (AR) remain unclear. Studies have indicated that decellularized scaffolds stimulate the differentiation of fibroblasts into myofibroblasts and promote the accumulation of the extracellular matrix (ECM). In the present study, the impact of ECM changes following AR on vascular smooth muscle cell (VSMC) phenotypes was investigated. VSMCs were co-cultured with normal or calcified decellularized arterial scaffolds. The expression levels of α-smooth muscle actin (α-SMA) and osteopontin (OPN) were measured at 2, 5, 10, 15 and 21 days following the establishment of the co-culture systems. The expression of α-SMA in the normal co-culture group was significantly increased compared with that in the calcified arterial decellularized scaffold co-culture group (P<0.05 and P<0.001). In addition, the expression of OPN in the AR co-culture group was significantly increased compared with the normal co-culture group (P<0.05 and P<0.001). To conclude, the calcified decellularized arterial scaffolds impact VSMC transformation by downregulating α-SMA expression and upregulating OPN expression (P<0.001). To the best of our knowledge, the present study is the first study that co-cultured VSMCs with normal or calcified decellularized arterial scaffolds.

• Publication year

  2019

• Venue

  Experimental and Therapeutic Medicine

• Publication date

  2019-05-29

• Fields of study

  Medicine, Materials Science, Engineering

• Identifiers
  DOI 10.3892/ETM.2019.7626PMID 31281450PMCID PMC6580105
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Tissue Transglutaminase Modulates Vascular Stiffness and Function Through Crosslinking‐Dependent and Crosslinking‐Independent Functions

  2017cited by this paper
• Vascular Smooth Muscle Cells and Arterial Stiffening: Relevance in Development, Aging, and Disease.

  2017cited by this paper
• Pathology of Human Coronary and Carotid Artery Atherosclerosis and Vascular Calcification in Diabetes Mellitus.

  2017cited by this paper
• The Role of AGE/RAGE Signaling in Diabetes-Mediated Vascular Calcification

  2016cited by this paper
• Osteopontin plays a key role in vascular smooth muscle cell proliferation via EGFR-mediated activation of AP-1 and C/EBPβ pathways.

  2016cited by this paper
• Vascular calcification in type-2 diabetes and cardiovascular disease: Integrative roles for OPG, RANKL and TRAIL.

  2016cited by this paper
• Naturally Occurring Extracellular Matrix Scaffolds for Dermal Regeneration: Do They Really Need Cells?

  2015cited by this paper
• Vascular smooth muscle cell calcification is mediated by regulated exosome secretion.

  2015cited by this paper
• Vascular calcification: from pathophysiology to biomarkers.

  2015cited by this paper
• The dark and bright side of atherosclerotic calcification.

  2015cited by this paper
• Impaired LRP6-TCF7L2 Activity Enhances Smooth Muscle Cell Plasticity and Causes Coronary Artery Disease.

  2015cited by this paper
• Mindin regulates vascular smooth muscle cell phenotype and prevents neointima formation.

  2015cited by this paper
• Functional ultrasound imaging for assessment of extracellular matrix scaffolds used for liver organoid formation.

  2013cited by this paper
• Evaluation of two decellularization methods in the development of a whole‐organ decellularized rat liver scaffold

  2013cited by this paper
• Vascular fibrosis in atherosclerosis.

  2013cited by this paper
• Extracellular matrix presentation modulates vascular smooth muscle cell mechanotransduction.

  2012cited by this paper
• Epigenetic control of smooth muscle cell differentiation and phenotypic switching in vascular development and disease.

  2012cited by this paper
• Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds.

  2011cited by this paper
• Matrix metalloproteinases involvement in pathologic conditions.

  2010cited by this paper
• Vascular calcification: pathobiology of a multifaceted disease.

  2008cited by this paper
• TGF‐β signaling and the fibrotic response

  2004cited by this paper
• The extracellular matrix as a scaffold for tissue reconstruction.

  2002cited by this paper
• Connective tissue growth factor: A new and important player in the pathogenesis of fibrosis

  2002cited by this paper
• Role of the renin-angiotensin system in vascular diseases: expanding the field.

  2001cited by this paper
• Porcine small intestinal submucosa (SIS): a bioscaffold supporting in vitro primary human epidermal cell differentiation and synthesis of basement membrane proteins.

  2001cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Functional tissue engineering: the role of biomechanics.

  2000cited by this paper

• Study on biomarkers of homocysteine-induced transformation of vascular smooth muscle cells into foam cells

  2026cites this paper
• The protective role of adiponectin in acute aortic dissection through regulating the Calcineurin-NFAT/miR-133a signaling pathway: electrochemical sensor protein detection

  2025cites this paper
• Echinochrome A inhibits HMGB1-induced vascular smooth muscle cell migration by suppressing osteopontin expression

  2024cites this paper
• Cathepsin D Attenuates the Proliferation of Vascular Smooth Muscle Cells Induced by the AGE/RAGE Pathway by Suppressing the ERK Signal.

  2023cites this paper
• Astragaloside IV induces the protective effect of bone marrow mesenchymal stem cells derived exosomes in acute myocardial infarction by inducing angiogenesis and inhibiting apoptosis

  2023cites this paper
• GDF11 Is a Novel Protective Factor Against Vascular Calcification

  2022cites this paper
• Single-Cell RNA Sequencing Technology Revealed the Pivotal Role of Fibroblast Heterogeneity in Angiotensin II-Induced Abdominal Aortic Aneurysms

  2022cites this paper
• Histopathological study of JNK in venous wall of patients with chronic venous insufficiency related to osteogenesis process

  2021cites this paper
• Single-cell RNA Sequencing Technology Revealed the Pivotal Role of Fibroblast Heterogeneity in Ang II-Induced Abdominal Aortic Aneurysms

  2021cites this paper
• An Overview of miRNAs Involved in PASMC Phenotypic Switching in Pulmonary Hypertension

  2021cites this paper
• A microRNA-17-5p/homeobox B13 axis participates in the phenotypic modulation of vascular smooth muscle cells

  2021influential citation