Differential translocation of protein precursors across SecY-deficient membranes of Escherichia coli: SecY is not obligatorily required for translocation of certain secretory proteins in vitro

No abstract is available for this paper.

• Publication year

  1997

• Venue

  Journal of Bacteriology

• Publication date

  1997-12-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1128/jb.179.23.7386-7393.1997PMID 9393703PMCID PMC179689
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Identification and Characterization of Protease-Resistant SecA Fragments: SecA Has Two Membrane-Integral Forms

  1998cited by this paper
• The E. coli signal recognition particle is required for the insertion of a subset of inner membrane proteins.

  1997cited by this paper
• Distinct catalytic roles of the SecYE, SecG and SecDFyajC subunits of preprotein translocase holoenzyme

  1997cited by this paper
• SecE-depleted Membranes of Escherichia coli Are Active

  1997cited by this paper
• Co‐translational protein targeting catalyzed by the Escherichia coli signal recognition particle and its receptor

  1997cited by this paper
• prlA suppressors in Escherichia coli relieve the proton electrochemical gradient dependency of translocation of wild-type precursors.

  1996cited by this paper
• Common Principles of Protein Translocation Across Membranes

  1996influential reference
• Escherichia coli Preprotein Translocase*

  1996influential reference
• A Significant Fraction of Functional SecA Is Permanently Embedded in the Membrane

  1996cited by this paper
• Involvement of stress protein PspA (phage shock protein A) of Escherichia coli in maintenance of the protonmotive force under stress conditions.

  1996cited by this paper
• SecA membrane cycling at SecYEG is driven by distinct ATP binding and hydrolysis events and is regulated by SecD and SecF.

  1995cited by this paper
• SecYEG and SecA Are the Stoichiometric Components of Preprotein Translocase (*)

  1995cited by this paper
• The allele‐specific synthetic lethality of prlA‐prlG double mutants predicts interactive domains of SecY and SecE.

  1995cited by this paper
• FtsH is required for proteolytic elimination of uncomplexed forms of SecY, an essential protein translocase subunit.

  1995cited by this paper
• Preferential interaction of Sec-G with Sec-E stabilizes an unstable Sec-E derivative in the Escherichia coli cytoplasmic membrane.

  1995cited by this paper
• SecD and SecF are required for the proton electrochemical gradient stimulation of preprotein translocation.

  1994cited by this paper
• PrlA and PrlG suppressors reduce the requirement for signal sequence recognition

  1994cited by this paper
• SecA promotes preprotein translocation by undergoing ATP-driven cycles of membrane insertion and deinsertion.

  1994cited by this paper
• SecA protein is exposed to the periplasmic surface of the E. coli inner membrane in its active state.

  1994cited by this paper
• The SecY protein family: comparative analysis and phylogenetic relationships.

  1994cited by this paper
• Disruption of the gene encoding p12 (SecG) reveals the direct involvement and important function of SecG in the protein translocation of Escherichia coli at low temperature.

  1994cited by this paper
• Genetic analysis of SecY: additional export-defective mutations and factors affecting their phenotypes

  1994cited by this paper
• Reconstitution of an efficient protein translocation machinery comprising SecA and the three membrane proteins, SecY, SecE, and SecG (p12).

  1994cited by this paper
• SecF stabilizes SecD and SecY, components of the protein translocation machinery of the Escherichia coli cytoplasmic membrane

  1994influential reference
• SecD is involved in the release of translocated secretory proteins from the cytoplasmic membrane of Escherichia coli.

  1993cited by this paper
• SecA protein is required for translocation of a model precursor protein into inverted vesicles of Escherichia coli plasma membrane.

  1993influential reference
• A novel membrane protein involved in protein translocation across the cytoplasmic membrane of Escherichia coli.

  1993cited by this paper
• The SecA and SecY subunits of translocase are the nearest neighbors of a translocating preprotein, shielding it from phospholipids.

  1993cited by this paper
• Purified Escherichia coli preprotein translocase catalyzes multiple cycles of precursor protein translocation.

  1993cited by this paper
• The complete general secretory pathway in gram-negative bacteria.

  1993cited by this paper
• PrlA suppressor mutations cluster in regions corresponding to three distinct topological domains.

  1993cited by this paper
• Cloning and molecular characterization of the secY genes from Bacillus licheniformis and Staphylococcus carnosus: comparative analysis of nine members of the SecY family

  1992cited by this paper
• The carboxyl-terminal region of SecE interacts with SecY and is functional in the reconstitution of protein translocation activity in Escherichia coli.

  1992cited by this paper
• SecY, SecE, and band 1 form the membrane-embedded domain of Escherichia coli preprotein translocase.

  1992cited by this paper
• In vitro protein translocation into Escherichia coli inverted membrane vesicles.

  1991cited by this paper
• Purification of SecE and reconstitution of SecE‐dependent protein translocation activity

  1991cited by this paper
• SecY is an indispensable component of the protein secretory machinery of Escherichia coli.

  1991cited by this paper
• Reconstitution of a protein translocation system containing purified SecY, SecE, and SecA from Escherichia coli.

  1991cited by this paper
• Molecular chaperones and protein translocation across the Escherichia coli inner membrane

  1991cited by this paper
• Genetic analysis of protein export in Escherichia coli.

  1990cited by this paper
• SecE‐dependent overproduction of SecY in Escherichia coli

  1990influential reference
• PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli.

  1990influential reference
• The purified E. coli integral membrane protein SecY/E is sufficient for reconstitution of SecA-dependent precursor protein translocation.

  1990cited by this paper
• Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins.

  1990influential reference
• Characterization of cold-sensitive secY mutants of Escherichia coli

  1990influential reference
• PrlA is important for the translocation of exported proteins across the cytoplasmic membrane of Escherichia coli.

  1989influential reference
• Purification of the Escherichia coli secB gene product and demonstration of its activity in an in vitro protein translocation system.

  1989cited by this paper
• Temperature-dependent insertion of prolipoprotein into Escherichia coli membrane vesicles and requirements for ATP, soluble factors, and functional SecY protein for the overall translocation process

  1989cited by this paper
• SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

  1989influential reference
• SecA protein is required for secretory protein translocation into E. coli membrane vesicles.

  1988cited by this paper
• SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.

  1988cited by this paper
• Biochemical evidence for the secY24 defect in Escherichia coli protein translocation and its suppression by soluble cytoplasmic factors.

  1987cited by this paper
• Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli.

  1987cited by this paper
• Roles of H+-ATPase and proton motive force in ATP-dependent protein translocation in vitro

  1986cited by this paper
• Genetic analysis of protein export in Escherichia coli K12.

  1985cited by this paper
• ATP is essential for protein translocation into Escherichia coli membrane vesicles.

  1985cited by this paper
• Characterization of an amber mutation in the structural gene for ribosomal protein L15, which impairs the expression of the protein export gene, secY, in Escherichia coli

  1984cited by this paper
• Energy-requiring translocation of the OmpA protein and alkaline phosphatase of Escherichia coli into inner membrane vesicles

  1984cited by this paper
• Characterization of an amber mutation in the structural gene for ribosomal protein L15, which impairs the expression of the protein export gene, secY, in Escherichia coli.

  1984influential reference
• A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature‐sensitive secY mutant.

  1984influential reference
• A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins.

  1983influential reference
• The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.

  1983cited by this paper
• Genetic analysis of protein export in Escherichia coli.

  1983cited by this paper
• Suppressor mutations that restore export of a protein with a defective signal sequence.

  1981cited by this paper
• Transposition and fusion of the lac genes to selected promoters in Escherichia coli using bacteriophage lambda and Mu.

  1976influential reference
• A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.

  1976cited by this paper
• A protonmotive force drives ATP synthesis in bacteria.

  1974cited by this paper
• Energy coupling in membrane vesicles of Escherichia coli. I. Accumulation of metabolites in response to an electrical potential.

  1974cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Isolation and characterization of ribonuclease I mutants of Escherichia coli.

  1966influential reference
• MUTANTS OF ESCHERICHIA COLI REQUIRING METHIONINE OR VITAMIN B12

  1950cited by this paper

• Uncovering the membrane-integrated SecAN protein that plays a key role in translocating nascent outer membrane proteins.

  2022cites this paper
• The transmembrane supercomplex mediating the biogenesis of OMPs in Gram‐negative bacteria assumes a circular conformational change upon activation

  2020cites this paper
• Structural insights into the mechanism of a novel protein targeting pathway in Gram‐negative bacteria

  2020cites this paper
• New Insights into Amino-Terminal Translocation as Revealed by the Use of YidC and Sec Depletion Strains.

  2019cites this paper
• SecA inhibitors as potential antimicrobial agents: differential actions on SecA-only and SecA-SecYEG protein-conducting channels

  2018cites this paper
• Discovery of a shortened version of SecA (SecAN) that conceivably functions as a protein-conducting channel

  2017cites this paper
• Dissecting structures and functions of SecA-only protein-conducting channels: ATPase, pore structure, ion channel activity, protein translocation, and interaction with SecYEG/SecDF•YajC

  2017cites this paper
• Revealing a shortened version of SecA (SecAN) that conceivably functions as a protein-conducting channel in the bacterial cytoplasmic membrane

  2017cites this paper
• Revelation of a Novel Protein Translocon in Bacterial Plasma Membrane

  2017cites this paper
• A Shortened Version Of SecA (SecAN) Functions As The Protein-Conducting Channel For Nascent β-Barrel Outer Membrane Proteins

  2017cites this paper
• Biphasic actions of SecA inhibitors on Prl/Sec suppressors: Possible physiological roles of SecA-only channels.

  2017cites this paper
• Design, Synthesis and Evaluation of Triazole‐Pyrimidine Analogues as SecA Inhibitors

  2016cites this paper
• SecA: a potential antimicrobial target.

  2015cites this paper
• The Bacterial Sec Pathway of Protein Export

  2015cites this paper
• Reconstitution of functionally efficient SecA-dependent protein-conducting channels: transformation of low-affinity SecA-liposome channels to high-affinity SecA-SecYEG-SecDF·YajC channels.

  2013cites this paper
• Inhibitors of SecA as Potential Antimicrobial Agents

  2013cites this paper
• Phospholipids Induce Conformational Changes of SecA to Form Membrane-Specific Domains: AFM Structures and Implication on Protein-Conducting Channels

  2013cites this paper
• Specificity of SecYEG for PhoA precursors and SecA homologs on SecA protein-conducting channels.

  2013cites this paper
• Escherichia coli Membranes Depleted of SecYEG Elicit SecA-Dependent Ion-Channel Activity but Lose Signal Peptide Specificity

  2012cites this paper
• Prediction of Lipid-Binding Regions in Cytoplasmic and Extracellular Loops of Membrane Proteins as Exemplified by Protein Translocation Membrane Proteins

  2012cites this paper
• Characterization of Structure and Function of SECA Domains

  2011cites this paper
• Electrophysiological Characterization of SecA-dependent Protein-conducting Channel

  2011cites this paper
• SecA Alone Can Promote Protein Translocation and Ion Channel Activity

  2011cites this paper
• Characterization of SecA1 and SecA2 from Gram-Positive Pathogens and Discovery of Novel SecA Inhibitors

  2011cites this paper
• Electrophysiological Studies in Xenopus Oocytes for the Opening of Escherichia coli SecA-Dependent Protein-Conducting Channels

  2006cites this paper
• Loss of Outer Membrane Proteins without Inhibition of Lipid Export in an Escherichia coli YaeT Mutant*

  2005cites this paper
• Ring-like pore structures of SecA: Implication for bacterial protein-conducting channels

  2003cites this paper
• Cell and process design for targeting of recombinant protein into the culture medium of Escherichia coli

  2003cites this paper
• DnaK Promotes the Selective Export of Outer Membrane Protein Precursors in SecA-deficient Escherichia coli*

  2002cites this paper
• Protein traffic in bacteria: multiple routes from the ribosome to and across the membrane.

  2001cites this paper
• Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map

  1998cites this paper
• Identification and Characterization of Protease-Resistant SecA Fragments: SecA Has Two Membrane-Integral Forms

  1998cites this paper
• Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map

  1998cites this paper
• Old and new pathways of protein export in chloroplasts and bacteria.

  1998cites this paper
• Targeting and assembly of periplasmic and outer-membrane proteins in Escherichia coli.

  1998cites this paper