Genetic analysis of protein export in Escherichia coli.

No abstract is available for this paper.

• Publication year

  1990

• Venue

  Annual Review of Genetics

• Publication date

  Unknown publication date

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/0076-6879(83)97114-8PMID 2088168
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Genetic characterization of a gene for prolipoprotein signal peptidase in Escherichia coli

  2004cited by this paper
• Identification of the secY (prlA) gene product involved in protein export in Escherichia coli

  2004cited by this paper
• The use of extragenic suppressors to define genes involved in protein export in Escherichia coli

  2004cited by this paper
• Increased expression of the bifunctional protein PrlF suppresses overproduction lethality associated with exported beta-galactosidase hybrid proteins in Escherichia coli

  1990cited by this paper
• Enhancement of protein translocation across the membrane by specific mutations in the hydrophobic region of the signal peptide

  1990cited by this paper
• Sequence and transcriptional pattern of the essential Escherichia coli secE-nusG operon

  1990cited by this paper
• Protein export in bacteria: An overview

  1990cited by this paper
• Temperature-sensitive sec mutants of Escherichia coli: inhibition of protein export at the permissive temperature

  1989cited by this paper
• Mutations that improve export of maltose-binding protein in SecB- cells of Escherichia coli

  1989cited by this paper
• Model for signal sequence recognition from amino-acid sequence of 54K subunit of signal recognition particle

  1989cited by this paper
• SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

  1989cited by this paper
• SecB functions as a cytosolic signal recognition factor for protein export in E. coli.

  1989cited by this paper
• Three pure chaperone proteins of Escherichia coli‐‐SecB, trigger factor and GroEL‐‐form soluble complexes with precursor proteins in vitro.

  1989cited by this paper
• PrlA is important for the translocation of exported proteins across the cytoplasmic membrane of Escherichia coli.

  1989cited by this paper
• Proton motive force-dependent and -independent protein translocation revealed by an efficient in vitro assay system of Escherichia coli.

  1989cited by this paper
• Cytosolic factor purified from Escherichia coli is necessary and sufficient for the export of a preprotein and is a homotetramer of SecB.

  1989cited by this paper
• SecA protein, a peripheral protein of the Escherichia coli plasma membrane, is essential for the functional binding and translocation of proOmpA.

  1989cited by this paper
• Unity in function in the absence of consensus in sequence: role of leader peptides in export.

  1989cited by this paper
• Illicit secretion of a cytoplasmic protein into the periplasm of Escherichia coli requires a signal peptide plus a portion of the cognate secreted protein. Demarcation of the critical region of the mature protein.

  1989cited by this paper
• The mature portion of Escherichia coli maltose-binding protein (MBP) determines the dependence of MBP on SecB for export

  1989cited by this paper
• Genetic studies on the inability of beta-galactosidase to be translocated across the Escherichia coli cytoplasmic membrane

  1989cited by this paper
• A high concentration of SecA allows proton motive force-independent translocation of a model secretory protein into Escherichia coli membrane vesicles.

  1989cited by this paper
• Identification of amino acid sequences that can function as translocators of β‐lactamase in Escherichia coli

  1989cited by this paper
• Polypeptide chain binding proteins: catalysts of protein folding and related processes in cells.

  1989cited by this paper
• Export of Escherichia coli alkaline phosphatase attached to an integral membrane protein, SecY.

  1989cited by this paper
• Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli.

  1989cited by this paper
• The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.

  1989cited by this paper
• Saccharomyces cerevisiae and Schizosaccharomyces pombe contain a homologue to the 54-kD subunit of the signal recognition particle that in S. cerevisiae is essential for growth

  1989cited by this paper
• Multiple genes are required for proper insertion of secretory proteins into the endoplasmic reticulum in yeast

  1989cited by this paper
• Physiological role during export for the retardation of folding by the leader peptide of maltose-binding protein.

  1989cited by this paper
• A conservative amino acid substitution, arginine for lysine, abolishes export of a hybrid protein in Escherichia coli. Implications for the mechanism of protein secretion.

  1989cited by this paper
• SecA protein is directly involved in protein secretion in Escherichia coli

  1989cited by this paper
• SEC62 encodes a putative membrane protein required for protein translocation into the yeast endoplasmic reticulum

  1989cited by this paper
• Conditionally lethal amber mutations in the leader peptidase gene of Escherichia coli

  1989cited by this paper
• SecA protein autogenously represses its own translation during normal protein secretion in Escherichia coli

  1989cited by this paper
• Novel secA alleles improve export of maltose-binding protein synthesized with a defective signal peptide

  1989cited by this paper
• In vitro analysis of the process of translocation of OmpA across the Escherichia coli cytoplasmic membrane. A translocation intermediate accumulates transiently in the absence of the proton motive force.

  1989cited by this paper
• New suppressors of signal-sequence mutations, prlG, are linked tightly to the secE gene of Escherichia coli.

  1989cited by this paper
• Purification of the Escherichia coli secB gene product and demonstration of its activity in an in vitro protein translocation system.

  1989cited by this paper
• PrlC, a suppressor of signal sequence mutations in Escherichia coli, can direct the insertion of the signal sequence into the membrane.

  1989cited by this paper
• Analysis of mutational alterations in the hydrophilic segment of the maltose-binding protein signal peptide

  1989cited by this paper
• Homology of 54K protein of signal-recognition particle, docking protein and two E. coli proteins with putative GTP–binding domains

  1989cited by this paper
• Effects of mutations in heat‐shock genes groES and groEL on protein export in Escherichia coli.

  1989cited by this paper
• A yeast gene important for protein assembly into the endoplasmic reticulum and the nucleus has homology to DnaJ, an Escherichia coli heat shock protein

  1989cited by this paper
• Escherichia coli SecB protein associates with exported protein precursors in vivo.

  1989cited by this paper
• Binding of a soluble factor of Escherichia coli to preproteins does not require ATP and appears to be the first step in protein export.

  1989cited by this paper
• Characterization of the Escherichia coli protein-export gene secB.

  1989cited by this paper
• Purified secB protein of Escherichia coli retards folding and promotes membrane translocation of the maltose-binding protein in vitro.

  1988cited by this paper
• Distinct mutation sites in prlA suppressor mutant strains of Escherichia coli respond either to suppression of signal peptide mutations or to blockage of staphylokinase processing

  1988cited by this paper
• Accumulation of LamB-LacZ hybrid proteins in intracytoplasmic membrane-like structures in Escherichia coli K12.

  1988cited by this paper
• Alteration of the amino terminus of the mature sequence of a periplasmic protein can severely affect protein export in Escherichia coli.

  1988cited by this paper
• Human SRP RNA and E. coli 4.5S RNA contain a highly homologous structural domain.

  1988cited by this paper
• A subfamily of stress proteins facilitates translocation of secretory and mitochondrial precursor polypeptides

  1988cited by this paper
• 70K heat shock related proteins stimulate protein translocation into microsomes

  1988cited by this paper
• Role of the leader peptide of maltose-binding protein in two steps of the export process

  1988cited by this paper
• Efficient in vitro translocation into Escherichia coli membrane vesicles of a protein carrying an uncleavable signal peptide. Characterization of the translocation process.

  1988cited by this paper
• SecA protein is required for secretory protein translocation into E. coli membrane vesicles.

  1988cited by this paper
• Protein unfolding and the energetics of protein translocation across biological membranes.

  1988cited by this paper
• Transient association of newly synthesized unfolded proteins with the heat-shock GroEL protein

  1988cited by this paper
• Nucleotide sequence of the secA gene and secA(Ts) mutations preventing protein export in Escherichia coli

  1988cited by this paper
• Modulation of folding pathways of exported proteins by the leader sequence.

  1988cited by this paper
• Inhibition and resumption of processing of the staphylokinase in some Escherichia coli prlA suppressor mutants.

  1988cited by this paper
• Introduction of basic amino acid residues after the signal peptide inhibits protein translocation across the cytoplasmic membrane of Escherichia coli. Relation to the orientation of membrane proteins.

  1988cited by this paper
• The antifolding activity of SecB promotes the export of the E. coli maltose-binding protein.

  1988cited by this paper
• Regulation of the Escherichia coli secA gene by protein secretion defects: analysis of secA, secB, secD, and secY mutants

  1988cited by this paper
• Retardation of folding as a possible means of suppression of a mutation in the leader sequence of an exported protein.

  1988cited by this paper
• SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.

  1988cited by this paper
• Effects of Escherichia coli secB mutations on pre-maltose binding protein conformation and export kinetics.

  1988cited by this paper
• A mutation affecting the regulation of a secA-lacZ fusion defines a new sec gene.

  1988cited by this paper
• Export of protein: a biochemical view.

  1987cited by this paper
• Trigger factor: a soluble protein that folds pro-OmpA into a membrane-assembly-competent form.

  1987cited by this paper
• Characterization and in vivo cloning of prlC, a suppressor of signal sequence mutations in Escherichia coli K12.

  1987cited by this paper
• Many random sequences functionally replace the secretion signal sequence of yeast invertase.

  1987cited by this paper
• Evidence for the involvement of ATP in co-translational protein translocation

  1987cited by this paper
• Proteins as molecular chaperones

  1987cited by this paper
• A small hydrophobic domain anchors leader peptidase to the cytoplasmic membrane of Escherichia coli.

  1987cited by this paper
• On the translocation of proteins across membranes.

  1987cited by this paper
• Suppression of a signal sequence mutation by an amino acid substitution in the mature portion of the maltose-binding protein

  1987cited by this paper
• secD, a new gene involved in protein export in Escherichia coli

  1987cited by this paper
• Mutations in the gene for EF-G reduce the requirement for 4.5S RNA in the growth of E. coli.

  1987cited by this paper
• Signal sequence mutations that alter coupling of secretion and translation of an Escherichia coli outer membrane protein

  1987cited by this paper
• In vitro translocation of protein across Escherichia coli membrane vesicles requires both the proton motive force and ATP.

  1987cited by this paper
• Identification of the genes in the Escherichia coli ileS-lsp operon. Analysis of multiple polycistronic mRNAs made in vivo.

  1987cited by this paper
• A yeast mutant defective at an early stage in import of secretory protein precursors into the endoplasmic reticulum

  1987cited by this paper
• Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli.

  1987cited by this paper
• Sequence analysis, transcriptional organization, and insertional mutagenesis of the envA gene of Escherichia coli

  1987cited by this paper
• Export of unprocessed precursor maltose-binding protein to the periplasm of Escherichia coli cells

  1987cited by this paper
• Biochemical evidence for the secY24 defect in Escherichia coli protein translocation and its suppression by soluble cytoplasmic factors.

  1987cited by this paper
• Mutational alterations affecting the export competence of a truncated but fully functional maltose-binding protein signal peptide

  1987cited by this paper
• Effects of signal sequence mutations on the kinetics of alkaline phosphatase export to the periplasm in Escherichia coli

  1986cited by this paper
• The secY protein can act post-translationally to promote bacterial protein export.

  1986cited by this paper
• Suppression of growth and protein secretion defects in Escherichia coli secA mutants by decreasing protein synthesis

  1986cited by this paper
• Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export.

  1986cited by this paper
• Net N-C charge imbalance may be important for signal sequence function in bacteria.

  1986cited by this paper
• Correlation of competence for export with lack of tertiary structure of the mature species: a study in vivo of maltose-binding protein in E. coli.

  1986cited by this paper
• Effects of nucleotides on ATP-dependent protein translocation into Escherichia coli membrane vesicles

  1986cited by this paper
• Roles of H+-ATPase and proton motive force in ATP-dependent protein translocation in vitro

  1986cited by this paper
• malM, a new gene of the maltose regulon in Escherichia coli K12. II. Mutations affecting the signal peptide of the MalM protein.

  1986cited by this paper
• Effect of amino acid substitutions at the signal peptide cleavage site of the Escherichia coli major outer membrane lipoprotein.

  1986cited by this paper
• A genetic approach to analyzing membrane protein topology.

  1986cited by this paper

• Physical mechanisms of the Sec machinery operation

  2024cites this paper
• Pro-SMP finder–A systematic approach for discovering small membrane proteins in prokaryotes

  2024cites this paper
• Signal peptide stabilizes folding and inhibits misfolding of serum amyloid A

  2022cites this paper
• Uncovering the membrane-integrated SecAN protein that plays a key role in translocating nascent outer membrane proteins.

  2022cites this paper
• Pushing the Envelope: The Mysterious Journey Through the Bacterial Secretory Machinery, and Beyond

  2021cites this paper
• Structure, Assembly, and Function of Tripartite Efflux and Type 1 Secretion Systems in Gram-Negative Bacteria

  2021cites this paper
• Expression of the Bacillus subtilis TasA signal peptide leads to cell death in Escherichia coli due to inefficient cleavage by LepB.

  2021cites this paper
• Fates of Sec, Tat, and YidC Translocases in Mitochondria and Other Eukaryotic Compartments

  2021cites this paper
• Structural insights into the mechanism of a novel protein targeting pathway in Gram‐negative bacteria

  2020cites this paper
• Cyclin-Dependent Kinase Inhibitor Gene TaICK1 acts as a Potential Contributor to Wheat Male Sterility induced by a Chemical Hybridizing Agent

  2020cites this paper
• Staphylococcal Protein Secretion and Envelope Assembly

  2019cites this paper
• CdsA is involved in biosynthesis of glycolipid MPIase essential for membrane protein integration in vivo

  2019cites this paper
• A plasmid‐borne Rap‐Phr system regulates sporulation of Bacillus thuringiensis in insect larvae

  2018cites this paper
• Proofreading of substrate by the Escherichia coli Twin Arginine Translocase

  2017cites this paper
• The Canonical and Accessory Sec System of Gram-positive Bacteria.

  2016cites this paper
• The Bacterial Sec Pathway of Protein Export

  2015cites this paper
• Role of the Carboxy Terminus of SecA in Iron Acquisition, Protein Translocation, and Virulence of the Bacterial Pathogen Acinetobacter baumannii

  2015cites this paper
• The Sec translocon mediated protein transport in prokaryotes and eukaryotes

  2014cites this paper
• Escherichia coli SecG Is Required for Residual Export Mediated by Mutant Signal Sequences and for SecY-SecE Complex Stability

  2014cites this paper
• Effect of Signal Peptide on Stability and Folding of Escherichia coli Thioredoxin

  2013cites this paper
• Protein translocation across the inner membrane of Gram-negative bacteria: the Sec and Tat dependent protein transport pathways.

  2013cites this paper
• Translocation of green fluorescent protein by comparative analysis with multiple signal peptides

  2012cites this paper
• Differential effect of YidC depletion on the membrane proteome of Escherichia coli under aerobic and anaerobic growth conditions

  2010cites this paper
• Influence of a Streptomyces lividans SecG functional analogue on protein secretion.

  2008cites this paper
• Characterization of the interaction between E. coli FtsY and the SecYEG complex

  2007cites this paper
• Topology Inversion of SecG Is Essential for Cytosolic SecA-dependent Stimulation of Protein Translocation*

  2007cites this paper
• Escherichia coli Twin Arginine (Tat) Mutant Translocases Possessing Relaxed Signal Peptide Recognition Specificities*

  2007cites this paper
• The SecY translocation complex: convergence of genetics and structure.

  2007cites this paper
• The structure of the Sec complex and the problem of protein translocation

  2006cites this paper
• A two-partner secretion system is involved in seed and root colonization and iron uptake by Pseudomonas putida KT2440.

  2006cites this paper
• Loss of Outer Membrane Proteins without Inhibition of Lipid Export in an Escherichia coli YaeT Mutant*

  2005cites this paper
• Sec-dependent protein translocation across biological membranes: evolutionary conservation of an essential protein transport pathway (Review)

  2005cites this paper
• Substrate recognition of type III secretion machines –testing the RNA signal hypothesis

  2005cites this paper
• Modeling the Effects of prl Mutations on the Escherichia coli SecY Complex

  2005cites this paper
• Preprotein Translocation through the Sec Translocon in Bacteria

  2005cites this paper
• Protein quality control in the bacterial periplasm

  2004cites this paper
• Sec-translocase mediated membrane protein biogenesis.

  2004cites this paper
• The archaeal Sec-dependent protein translocation pathway.

  2004cites this paper
• SecA is plastid-encoded in a red alga: implications for the evolution of plastid genomes and the thylakoid protein import apparatus

  2004cites this paper
• Functional activity of eukaryotic signal sequences in Escherichia coli: the ovalbumin family of serine protease inhibitors.

  2004cites this paper
• Cell biology: Shape-shifting protein channel

  2004cites this paper
• Genetic Analysis of Pathway Specificity during Posttranslational Protein Translocation across the Escherichia coli Plasma Membrane

  2003cites this paper
• The bacterial translocase: a dynamic protein channel complex

  2003cites this paper
• Colicin S8 export: extracellular and cytoplasmic colicin are different

  2003cites this paper
• The YSIRK-G/S Motif of Staphylococcal Protein A and Its Role in Efficiency of Signal Peptide Processing

  2003cites this paper
• Transport of the Intracisternal A-Type Particle Gag Polyprotein to the Endoplasmic Reticulum Is Mediated by the Signal Recognition Particle

  2003cites this paper
• Role for both DNA and RNA in GTP Hydrolysis by the Neisseria gonorrhoeae Signal Recognition Particle Receptor

  2003cites this paper
• Signal recognition particle-dependent protein targeting, universal to all kingdoms of life.

  2003cites this paper
• Recognition of secretory proteins in Escherichia coli requires signals in addition to the signal sequence and slow folding

  2002cites this paper
• Behaviour of topological marker proteins targeted to the Tat protein transport pathway

  2002cites this paper
• A novel class of secA alleles that exert a signal-sequence-dependent effect on protein export in Escherichia coli.

  2002cites this paper
• DnaK Promotes the Selective Export of Outer Membrane Protein Precursors in SecA-deficient Escherichia coli*

  2002cites this paper
• Yop Fusions to Tightly Folded Protein Domains and Their Effects on Yersinia enterocolitica Type III Secretion

  2002cites this paper
• Signal peptidases.

  2002cites this paper
• Cloning, expression, and functional characterization of the Mycobacterium tuberculosis secA gene.

  2002cites this paper
• Pore Formation and Function of Phosphoporin PhoE of Escherichia coli Are Determined by the Core Sugar Moiety of Lipopolysaccharide*

  2002cites this paper
• Secretion monitor, SecM, undergoes self-translation arrest in the cytosol.

  2001cites this paper
• The energetics of binding of a signal peptide to lipid bilayers: the role of bilayer properties.

  2001cites this paper
• Characterization of Pseudomonas aeruginosa Chitinase, a Gradually Secreted Protein

  2001cites this paper
• Protein secretion and the pathogenesis of bacterial infections.

  2001cites this paper
• The hepatitis C virus nonstructural protein 4B is an integral endoplasmic reticulum membrane protein.

  2001cites this paper
• Two distinctive cell binding patterns by vacuolating toxin fused with glutathione S-transferase: one high-affinity m1-specific binding and the other lower-affinity binding for variant m forms.

  2001cites this paper
• A SecY homologue is involved in chloroplast-encoded D1 protein biogenesis.

  2001cites this paper
• Protein traffic in bacteria: multiple routes from the ribosome to and across the membrane.

  2001cites this paper
• Expression and binding analysis of GST-VacA fusions reveals that the C-terminal approximately 100-residue segment of exotoxin is crucial for binding in HeLa cells.

  2000cites this paper
• Both transmembrane domains of SecG contribute to signal sequence recognition by the Escherichia coli protein export machinery

  2000cites this paper
• Fusion expression of Escherichia coli prlC gene and preparation of PrlC proteinase affinity column

  2000cites this paper
• Allele-specific suppression as a tool to study protein-protein interactions in bacteria.

  2000cites this paper
• Escherichia coli translocase: the unravelling of a molecular machine

  2000cites this paper
• Maturation and secretion of the non‐typable Haemophilus influenzae HMW1 adhesin: roles of the N‐terminal and C‐terminal domains

  2000cites this paper
• Proteins of the endoplasmic-reticulum-associated degradation pathway: domain detection and function prediction.

  2000cites this paper
• Protein Targeting to the Bacterial Cytoplasmic Membrane

  1999cites this paper
• Yersinia enterocolitica Type III Secretion

  1999cites this paper
• Cloning and nucleotide sequencing of the secA gene from coryneform bacteria.

  1999cites this paper
• Membrane deinsertion of SecA underlying proton motive force‐dependent stimulation of protein translocation

  1999cites this paper
• The engagement of Sec61p in the ER dislocation process.

  1999cites this paper
• The PrlA and PrlG phenotypes are caused by a loosened association among the translocase SecYEG subunits

  1999cites this paper
• The translocon: a dynamic gateway at the ER membrane.

  1999cites this paper
• Secretion of virulence factors by Escherichia coli.

  1999cites this paper
• Transcriptional Activation of ydeA, Which Encodes a Member of the Major Facilitator Superfamily, Interferes with Arabinose Accumulation and Induction of the Escherichia coli Arabinose PBADPromoter

  1999cites this paper
• SecA: the ubiquitous component of preprotein translocase in prokaryotes.

  1999cites this paper
• Surface Proteins of Gram-Positive Bacteria and Mechanisms of Their Targeting to the Cell Wall Envelope

  1999cites this paper
• Chloroplast FtsY, Chloroplast Signal Recognition Particle, and GTP Are Required to Reconstitute the Soluble Phase of Light-harvesting Chlorophyll Protein Transport into Thylakoid Membranes*

  1999cites this paper
• Bacillus subtilis Histone-like Protein, HBsu, Is an Integral Component of a SRP-like Particle That Can Bind theAlu Domain of Small Cytoplasmic RNA*

  1999cites this paper
• Chloroplast SecY Is Complexed to SecE and Involved in the Translocation of the 33-kDa but Not the 23-kDa Subunit of the Oxygen-evolving Complex*

  1999cites this paper
• Protein translocation into and across the bacterial plasma membrane and the plant thylakoid membrane.

  1999cites this paper
• Orientation of LamB signal peptides in bilayers: influence of lipid probes on peptide binding and interpretation of fluorescence quenching data.

  1999cites this paper
• Coupled structure change of SecA and SecG revealed by the synthetic lethality of the secAcsR11 and ΔsecG::kan double mutant

  1998cites this paper
• A Mutation in the Escherichia coli secY Gene That Produces Distinct Effects on Inner Membrane Protein Insertion and Protein Export*

  1998cites this paper
• Characterization of the C-terminal domain of Helicobacter pylori vacuolating toxin and its relationship with extracellular toxin production.

  1998cites this paper
• GSP-dependent protein secretion in gram-negative bacteria: the Xcp system of Pseudomonas aeruginosa.

  1998cites this paper
• Sec‐dependent membrane protein biogenesis: SecYEG, preprotein hydrophobicity and translocation kinetics control the stop‐transfer function

  1998cites this paper
• A Toxic Fusion Protein Accumulating between the Mitochondrial Membranes Inhibits Protein Assembly in Vivo *

  1998cites this paper
• Escherichia coli prlC gene encodes a trypsin-like proteinase regulating the cell cycle.

  1998cites this paper
• Missense mutations that inactivate Escherichia coli lac permease.

  1998cites this paper
• Modulation of the binding of signal peptides to lipid bilayers by dipoles near the hydrocarbon-water interface.

  1998cites this paper
• Secretion of the Haemophilus influenzae HMW1 and HMW2 adhesins involves a periplasmic intermediate and requires the HMWB and HMWC proteins

  1998cites this paper
• Protein translocation: tunnel vision.

  1998cites this paper
• Molecular cloning and functional characterization of SecE of a marine bacterium, Vibrio alginolyticus.

  1998cites this paper
• Deletion of algK in Mucoid Pseudomonas aeruginosa Blocks Alginate Polymer Formation and Results in Uronic Acid Secretion

  1998cites this paper