Biochemical evidence for the secY24 defect in Escherichia coli protein translocation and its suppression by soluble cytoplasmic factors.

No abstract is available for this paper.

• Publication year

  1987

• Venue

  Proceedings of the National Academy of Sciences of the United States of America

• Publication date

  1987-11-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1073/PNAS.84.21.7448PMID 2823262PMCID PMC299313
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• The use of extragenic suppressors to define genes involved in protein export in Escherichia coli

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• Identification of the secY (prlA) gene product involved in protein export in Escherichia coli

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• secD, a new gene involved in protein export in Escherichia coli

  1987cited by this paper
• Protein translocation into Escherichia coli membrane vesicles is inhibited by functional synthetic signal peptides.

  1987cited by this paper
• Signal peptidases.

  1986cited by this paper
• Suppressors of the secY24 mutation: identification and characterization of additional ssy genes in Escherichia coli

  1986cited by this paper
• Lack of effect of leader peptidase overproduction on the processing in vivo of exported proteins in Escherichia coli.

  1986cited by this paper
• The secY protein can act post-translationally to promote bacterial protein export.

  1986cited by this paper
• Both ATP and the electrochemical potential are required for optimal assembly of pro-OmpA into Escherichia coli inner membrane vesicles.

  1986cited by this paper
• Genetic studies on protein export in bacteria.

  1986cited by this paper
• Suppression of growth and protein secretion defects in Escherichia coli secA mutants by decreasing protein synthesis

  1986cited by this paper
• Altered translation initiation factor 2 in the cold‐sensitive ssyG mutant affects protein export in Escherichia coli.

  1986cited by this paper
• Biochemical studies of bacterial protein export.

  1986cited by this paper
• Translocation of nascent non-signal sequence protein in heated Escherichia coli.

  1986cited by this paper
• Kinetic analysis of lamB mutants suggests the signal sequence plays multiple roles in protein export.

  1986cited by this paper
• ATP is essential for protein translocation into Escherichia coli membrane vesicles.

  1985cited by this paper
• Multiple mechanisms of protein insertion into and across membranes.

  1985cited by this paper
• Identification of five new essential genes involved in the synthesis of a secreted protein in Escherichia coli

  1985cited by this paper
• Alkaline phosphatase and OmpA protein can be translocated posttranslationally into membrane vesicles of Escherichia coli

  1985cited by this paper
• The SecY membrane component of the bacterial protein export machinery: analysis by new electrophoretic methods for integral membrane proteins.

  1985cited by this paper
• Genetic analysis of protein export in Escherichia coli K12.

  1985cited by this paper
• Energy-requiring translocation of the OmpA protein and alkaline phosphatase of Escherichia coli into inner membrane vesicles

  1984cited by this paper
• The synthesis of export-defective proteins can interfere with normal protein export in Escherichia coli.

  1984cited by this paper
• The genetics and regulation of heat-shock proteins.

  1984cited by this paper
• A defined mutation in the protein export gene within the spc ribosomal protein operon of Escherichia coli: isolation and characterization of a new temperature‐sensitive secY mutant.

  1984cited by this paper
• Mutation that suppresses the protein export defect of the secY mutation and causes cold-sensitive growth of Escherichia coli

  1984cited by this paper
• Protein export in Escherichia coli requires a soluble activity.

  1984cited by this paper
• Export of protein in bacteria

  1984cited by this paper
• In vitro translocation of bacterial proteins across the plasma membrane of Escherichia coli.

  1984cited by this paper
• The spc ribosomal protein operon of Escherichia coli: sequence and cotranscription of the ribosomal protein genes and a protein export gene.

  1983cited by this paper
• Importance of secondary structure in the signal sequence for protein secretion.

  1983cited by this paper
• Effects of the complete removal of basic amino acid residues from the signal peptide on secretion of lipoprotein in Escherichia coli.

  1983cited by this paper
• Localization and processing of outer membrane and periplasmic proteins in Escherichia coli strains harboring export-specific suppressor mutations.

  1982cited by this paper
• Suppressor mutations that restore export of a protein with a defective signal sequence.

  1981cited by this paper
• E. coli mutant pleiotropically defective in the export of secreted proteins.

  1981cited by this paper
• The mechanism of protein secretion across membranes

  1980cited by this paper
• Escherichia coli mutants accumulating the precursor of a secreted protein in the cytoplasm

  1979cited by this paper
• Transient regulation of protein synthesis in Escherichia coli upon shift-up of growth temperature

  1978cited by this paper
• Conditional-lethal mutations that suppress genetic defects in morphogenesis by altering structural proteins.

  1975cited by this paper
• Properties of initiation-free polysomes of Escherichia coli.

  1973cited by this paper
• EFFECT OF TEMPERATURE ON THE COMPOSITION OF FATTY ACIDS IN ESCHERICHIA COLI

  1962cited by this paper

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• secE encodes an integral protein required for protein export in Escherichia coli

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• H+-pumping ATPase has little stimulatory effect on in vitro translocation of a model protein into Vibrio alginolyticus inside-out membrane vesicles.

  1997cites this paper
• Differential translocation of protein precursors across SecY-deficient membranes of Escherichia coli: SecY is not obligatorily required for translocation of certain secretory proteins in vitro

  1997cites this paper
• Membrane assembly in bacteria.

  1994cites this paper
• The complete general secretory pathway in gram-negative bacteria.

  1993cites this paper
• SecA insertion into phospholipids is stimulated by negatively charged lipids and inhibited by ATP: a monolayer study.

  1992cites this paper
• Membrane insertion of the mannitol permease of Escherichia coli occurs under conditions of impaired SecA function.

  1992cites this paper
• Chapter 2 Components involved in bacterial protein translocation

  1992cites this paper
• SecY and integral membrane components of the Escherichia coli protein translocation system

  1992cites this paper
• On protein translocation across bacterial cytoplasmic membranes

  1992cites this paper
• Biochemical analysis of the biogenesis and function of the Escherichia coli export factor SecY.

  1992cites this paper
• Molybdenum requirement for translocation of dimethyl sulfoxide reductase to the periplasmic space in a photodenitrifier, Rhodobacter sphaeroides f. sp. denitrificans

  1991cites this paper
• Negatively charged phospholipids restore prePhoE translocation across phosphatidylglycerol-depleted Escherichia coli inner membranes.

  1991cites this paper
• Isolation and analysis of dominant secA mutations in Escherichia coli

  1991cites this paper
• One of three transmembrane stretches is sufficient for the functioning of the SecE protein, a membrane component of the E. coli secretion machinery.

  1991cites this paper
• Yeast gene which suppresses the defect in protein export of a secY mutant of E. coli.

  1991cites this paper
• In vitro protein translocation into Escherichia coli inverted membrane vesicles.

  1991cites this paper
• SecY is an indispensable component of the protein secretory machinery of Escherichia coli.

  1991cites this paper
• Novel prlA alleles defective in supporting staphylokinase processing in Escherichia coli

  1991cites this paper
• Assembly and Secretion of Oligomeric Toxins

  1991cites this paper
• SecY, a multispanning integral membrane protein, contains a potential leader peptidase cleavage site

  1990cites this paper
• Thesec andprl genes ofEscherichia coli

  1990cites this paper
• SecY protein, a membrane-embedded secretion factor of E. coli, is cleaved by the ompT protease in vitro.

  1990cites this paper
• Engineering Escherichia coli to secrete heterologous gene products.

  1990cites this paper
• Protein translocationin vitro: Biochemical characterization of genetically defined translocation components

  1990cites this paper
• Characterization of cold-sensitive secY mutants of Escherichia coli

  1990cites this paper
• Genetic analysis of protein export in Escherichia coli.

  1990cites this paper
• Characterization of a region in mature LamB protein that interacts with a component of the export machinery of Escherichia coli.

  1990cites this paper
• Compartmentalization of mammalian proteins produced in Escherichia coli.

  1990cites this paper
• SecA protein: Autoregulated initiator of secretory precursor protein translocation across theE. coli plasma membrane

  1990cites this paper
• Increased expression of the bifunctional protein PrlF suppresses overproduction lethality associated with exported beta-galactosidase hybrid proteins in Escherichia coli

  1990cites this paper
• CHAPTER 13 – Energetic Aspects of Protein Insertion and Translocation into or across Membranes

  1990cites this paper
• Reconstitution of protein translocation from detergent-solubilized Escherichia coli inverted vesicles: PrlA protein-deficient vesicles efficiently translocate precursor proteins.

  1990cites this paper
• Structure, function, and biogenesis of SecY, an integral membrane protein involved in protein export

  1990cites this paper
• Azide-resistant mutants of Escherichia coli alter the SecA protein, an azide-sensitive component of the protein export machinery.

  1990cites this paper
• PrlA (SecY) and PrlG (SecE) interact directly and function sequentially during protein translocation in E. coli.

  1990cites this paper
• Isolation of a secY homologue from Bacillus subtilis: evidence for a common protein export pathway in eubacteria

  1990cites this paper
• Isolation of a yeast gene, SRH1, that encodes a homologue of the 54K subunit of mammalian signal recognition particle.

  1990cites this paper
• [The SecY protein of E. coli, an integral membrane protein involved in protein translocation].

  1989cites this paper
• SecA protein hydrolyzes ATP and is an essential component of the protein translocation ATPase of Escherichia coli.

  1989cites this paper
• Temperature-dependent insertion of prolipoprotein into Escherichia coli membrane vesicles and requirements for ATP, soluble factors, and functional SecY protein for the overall translocation process

  1989cites this paper
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  1989cites this paper
• PrlA is important for the translocation of exported proteins across the cytoplasmic membrane of Escherichia coli.

  1989cites this paper
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  1989cites this paper
• PrlC, a suppressor of signal sequence mutations in Escherichia coli, can direct the insertion of the signal sequence into the membrane.

  1989cites this paper
• Purification of the Escherichia coli secB gene product and demonstration of its activity in an in vitro protein translocation system.

  1989cites this paper
• Temperature-sensitive sec mutants of Escherichia coli: inhibition of protein export at the permissive temperature

  1989cites this paper
• The genetics of protein targeting in Escherichia coli K12

  1989cites this paper
• Specific recognition of the leader region of precursor proteins is required for the activation of translocation ATPase of Escherichia coli.

  1989cites this paper
• Signal sequences.

  1989cites this paper
• Site-specific antibodies against the PrlA (secY) protein of Escherichia coli inhibit protein export by interfering with plasma membrane binding of preproteins.

  1989cites this paper
• New suppressors of signal-sequence mutations, prlG, are linked tightly to the secE gene of Escherichia coli.

  1989cites this paper
• Insertion of proteins into bacterial membranes: mechanism, characteristics, and comparisons with the eucaryotic process.

  1989cites this paper
• Factors influencing the in vitro translocation of the Escherichia coli maltose-binding protein.

  1989cites this paper
• Insertion of proteins into bacterial membranes: mechanism, characteristics, and comparisons with the eucaryotic process

  1989cites this paper
• Detergent disruption of bacterial inner membranes and recovery of protein translocation activity.

  1989cites this paper
• Export of honeybee prepromelittin in Escherichia coli depends on the membrane potential but does not depend on proteins secA and secY.

  1989cites this paper
• The secE gene encodes an integral membrane protein required for protein export in Escherichia coli.

  1989cites this paper
• Two cellular components, PrlA and SecB, that recognize different sequence determinants are required for efficient protein export

  1988cites this paper
• Analysis of pullulanase secretion from Klebsiella pneumoniae strain K21

  1988cites this paper
• The "trigger factor cycle" includes ribosomes, presecretory proteins, and the plasma membrane.

  1988cites this paper
• SecA suppresses the temperature-sensitive SecY24 defect in protein translocation in Escherichia coli membrane vesicles.

  1988cites this paper
• In vivo and in vitro analysis of ptl1, a yeast ts mutant with a membrane‐associated defect in protein translocation.

  1988cites this paper
• SecA protein is required for secretory protein translocation into E. coli membrane vesicles.

  1988cites this paper
• Mechanisms for secretion of extracellular proteins by gram-negative bacteria.

  1988cites this paper
• Chapter 15. Novel Approaches Toward Discovery of Antibacterial Agents

  1988cites this paper
• ProOmpA spontaneously folds in a membrane assembly competent state which trigger factor stabilizes.

  1988cites this paper