Current trends in affinity-based monoliths in microextraction approaches: A review.

This article reviews the research contributions along the past five years concerning to monolithic materials for the development of affinity-based sorbents in the field of microextraction techniques. The first part of this paper includes an introduction regarding monolithic affinity media and information of different binding agents (such as immunoglobulin-binding proteins, enzymes, lectins, antibodies, aptamers, dyes and immobilized metal ions and nanoparticles, among others) that can act as affinity ligands. Then, the preparation of monoliths and ligand immobilization strategies as well as the different available formats (syringes, pipette tips, spin columns, capillaries, disks and microfluidic devices) for their use in micro-solid phase extraction are mentioned. On the basis of the binding agents used to prepare affinity monolithic-based sorbents, the last part of manuscript includes several analytical and preparative applications of these monoliths for sample preparation covering different fields (analytical chemistry, biotechnology, proteomics, etc.). Current trends and possible future directions of these affinity-based sorbents in sample preparation are also given.

• Publication year

  2019

• Venue

  Analytica Chimica Acta

• Publication date

  2019-11-25

• Fields of study

  Medicine, Materials Science, Chemistry

• Identifiers
  DOI 10.1016/J.ACA.2019.07.020PMID 31519228
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Monolith weak affinity chromatography for &mgr;g‐protein‐ligand interaction study

  2019cited by this paper
• Ti (IV) attached-phosphonic acid functionalized capillary monolith as a stationary phase for in-syringe-type fast and robust enrichment of phosphopeptides.

  2019cited by this paper
• Preparation and evaluation of highly hydrophilic aptamer-based hybrid affinity monolith for on-column specific discrimination of ochratoxin A.

  2019cited by this paper
• Highly hydrophilic polyhedral oligomeric silsesquioxane (POSS)-containing aptamer-modified affinity hybrid monolith for efficient on-column discrimination with low nonspecific adsorption.

  2019cited by this paper
• Preparation of aptamer-bound polyamine affinity monolithic column via a facile triazine-bridged strategy and application to on-column specific discrimination of ochratoxin A.

  2019cited by this paper
• 3D printed microfluidic devices with immunoaffinity monoliths for extraction of preterm birth biomarkers

  2018cited by this paper
• A facile AuNPs@aptamer-modified mercaptosiloxane-based hybrid affinity monolith with an unusually high coverage density of aptamer for on-column selective extraction of ochratoxin A.

  2018cited by this paper
• Potential of nanoparticle-based hybrid monoliths as sorbents in microextraction techniques.

  2018cited by this paper
• Monolith affinity chromatography for the rapid quantification of a single‐chain variable fragment immunotoxin

  2018cited by this paper
• Organic polymer‐based monolithic stationary phases with incorporated nanostructured materials for HPLC and CEC

  2018cited by this paper
• Paper-based immune-affinity arrays for detection of multiple mycotoxins in cereals

  2018cited by this paper
• Arginine homopeptides for plasmid DNA purification using monolithic supports.

  2018cited by this paper
• New In-Depth Analytical Approach of the Porcine Seminal Plasma Proteome Reveals Potential Fertility Biomarkers.

  2018cited by this paper
• Aptamer-based polyhedral oligomeric silsesquioxane (POSS)-containing hybrid affinity monolith prepared via a "one-pot" process for selective extraction of ochratoxin A.

  2018cited by this paper
• An aptamer@AuNP-modified POSS-polyethylenimine hybrid affinity monolith with a high aptamer coverage density for sensitive and selective recognition of ochratoxin A.

  2018cited by this paper
• Chromatographic characterisation of aptamer-modified poly(EDMA-co-GMA) monolithic disk format for protein binding and separation

  2018cited by this paper
• Biomimetic small peptide functionalized affinity monoliths for monoclonal antibody purification.

  2018cited by this paper
• Online screening of acetylcholinesterase inhibitors in natural products using monolith-based immobilized capillary enzyme reactors combined with liquid chromatography-mass spectrometry.

  2018cited by this paper
• Lectin attached affinity cryogels for amyloglucosidase adsorption

  2018cited by this paper
• Reversible Concanavalin A (Con A) ligands immobilization on metal chelated macroporous cellulose monolith and its selective adsorption for glycoproteins.

  2018cited by this paper
• High performance affinity chromatography and related separation methods for the analysis of biological and pharmaceutical agents.

  2018cited by this paper
• Selective Fishing for Peptides with Antibody-Immobilized Acrylate Monoliths, Coupled Online with NanoLC-MS.

  2018cited by this paper
• Specific enrichment of glycoproteins with polymer monolith functionalized with glycocluster grafted β-cyclodextrin.

  2017cited by this paper
• Application of Organic Monolithic Materials to Enantioseparation in Capillary Separation Techniques.

  2017cited by this paper
• Organo-silica hybrid capillary monolithic column with mesoporous silica particles for separation of small aromatic molecules

  2017cited by this paper
• Affinity monolith chromatography: A review of general principles and applications

  2017cited by this paper
• Polymethacrylate‐based monoliths as stationary phases for separation of biopolymers and immobilization of enzymes

  2017cited by this paper
• Semi‐high‐throughput isolation and N‐glycan analysis of human fibrinogen using monolithic supports bearing monoclonal anti‐human fibrinogen antibodies

  2017cited by this paper
• Poly (N‐acryloxysuccinimide‐co‐ethylene glycol dimethacrylate) precursor monolith and its post polymerization modification with alkyl ligands, trypsin and lectins for reversed‐phase chromatography, miniaturized enzyme reactors and lectin affinity chromatography, respectively

  2017cited by this paper
• Preparation and characterization of a Cu (II)‐IDA poly HEMA monolith syringe for proteomic applications

  2017cited by this paper
• Glycopolymer monoliths for affinity bioseparation of proteins in a continuous-flow system: glycomonoliths.

  2017cited by this paper
• Polymeric sorbents modified with gold and silver nanoparticles for solid-phase extraction of proteins followed by MALDI-TOF analysis

  2017cited by this paper
• Development of a metal/chelate polyhydroxyethylmethacrylate monolith capillary for selective depletion of immunoglobulin G from human plasma for proteomics.

  2017cited by this paper
• Preparation of organic monolithic columns in polytetrafluoroethylene tubes for reversed-phase liquid chromatography.

  2017cited by this paper
• Peanut agglutinin and β-cyclodextrin functionalized polymer monolith: Microextraction of IgG galactosylation coupled with online MS detection.

  2017cited by this paper
• A structure based plasma protein pre-fractionation using conjoint immobilized metal/chelate affinity (IMA) system.

  2017cited by this paper
• Porous monoliths for on-line sample preparation: A review.

  2017cited by this paper
• Aptamer-immobilized open tubular capillary column to capture circulating tumor cells for proteome analysis.

  2017cited by this paper
• Immobilization of sugars in supermacroporous cryogels for the purification of lectins by affinity chromatography.

  2017cited by this paper
• Development of immune-affinity 96 spots monolith array for multiple mycotoxins detection in food samples.

  2016cited by this paper
• Monoliths in capillary electrochromatography and capillary liquid chromatography in conjunction with mass spectrometry

  2016cited by this paper
• Advances in monoliths and related porous materials for microfluidics.

  2016cited by this paper
• Development of supermacroporous monolithic adsorbents for purifying lectins by affinity with sugars.

  2016cited by this paper
• A review on recent developments for biomolecule separation at analytical scale using microfluidic devices.

  2016cited by this paper
• Recent advances in the preparation and application of monolithic capillary columns in separation science.

  2016cited by this paper
• Depletion of human serum albumin in embryo culture media for in vitro fertilization using monolithic columns with immobilized antibodies

  2016cited by this paper
• The current state of the art of quantitative phosphoproteomics and its applications to diabetes research

  2016cited by this paper
• Glycopolymer-Functionalized Cryogels as Catch and Release Devices for the Pre-Enrichment of Pathogens.

  2016cited by this paper
• [PHEMA/PEI]-Cu(II) based immobilized metal affinity chromatography cryogels: Application on the separation of IgG from human plasma.

  2016cited by this paper
• Characterization of methacrylate chromatographic monoliths bearing affinity ligands.

  2016cited by this paper
• Solid-phase extraction based on ground methacrylate monolith modified with gold nanoparticles for isolation of proteins.

  2016cited by this paper
• Aptamer functionalized hydrophilic polymer monolith with gold nanoparticles modification for the sensitive detection of human α-thrombin.

  2016cited by this paper
• A one-step preparation method of monolithic enzyme reactor for highly efficient sample preparation coupled to mass spectrometry-based proteomics studies.

  2015cited by this paper
• Aptamer-based organic-silica hybrid affinity monolith prepared via "thiol-ene" click reaction for extraction of thrombin.

  2015cited by this paper
• Affinity binding of antibodies to supermacroporous cryogel adsorbents with immobilized protein A for removal of anthrax toxin protective antigen.

  2015cited by this paper
• Dye affinity cryogels for plasmid DNA purification.

  2015cited by this paper
• Mesoporous monoliths of inverse bicontinuous cubic phases of block copolymer bilayers

  2015cited by this paper
• Monolith immuno-affinity enrichment liquid chromatography tandem mass spectrometry for quantitative protein analysis of recombinant bovine somatotropin in serum

  2015cited by this paper
• Immobilization of alcohol dehydrogenase onto metal-chelated cryogels

  2015cited by this paper
• Biobased monoliths for adenovirus purification.

  2015cited by this paper
• TiO₂ nanoparticles functionalized monolithic capillary microextraction online coupled with inductively coupled plasma mass spectrometry for the analysis of Gd ion and Gd-based contrast agents in human urine.

  2015cited by this paper
• Development of a silica monolith modified with Fe3O4 nano-particles in centrifugal spin column format for the extraction of phosphorylated compounds.

  2015cited by this paper
• Selective capture of glycoproteins using lectin-modified nanoporous gold monolith.

  2015cited by this paper
• Dye functionalized cryogel columns for reversible lysozyme adsorption

  2015cited by this paper
• In-line coupling of an aptamer based miniaturized monolithic affinity preconcentration unit with capillary electrophoresis and Laser Induced Fluorescence detection.

  2015cited by this paper
• Macroporous cryogel based spin column with immobilized concanavalin A for isolation of glycoproteins

  2015cited by this paper
• Gold nanoparticles immobilized hydrophilic monoliths with variable functional modification for highly selective enrichment and on-line deglycosylation of glycopeptides.

  2015cited by this paper
• Aptamers as a replacement for antibodies in enzyme-linked immunosorbent assay.

  2015cited by this paper
• Affinity composite cryogel discs functionalized with Reactive Red 120 and Green HE 4BD dye ligands: application on the separation of human immunoglobulin G subclasses.

  2015cited by this paper
• Recent advances in nonpolar and polar organic monoliths for HPLC and CEC

  2015cited by this paper
• Supermacroporous polyHIPE and cryogel monolithic materials as stationary phases in separation science: a review

  2015cited by this paper
• Rapid and simple preparation of thiol-ene emulsion-templated monoliths and their application as enzymatic microreactors.

  2015cited by this paper
• Preparation of a monolith functionalized with zinc oxide nanoparticles and its application in the enrichment of fluoroquinolone antibiotics.

  2015cited by this paper
• Advances and recent trends in the field of monolithic columns for chromatography.

  2015cited by this paper
• Growth of a Highly Porous Coordination Polymer on a Macroporous Polymer Monolith Support for Enhanced Immobilized Metal Ion Affinity Chromatographic Enrichment of Phosphopeptides

  2014cited by this paper
• A Novel Affinity Disks for Bovine Serum Albumin Purification

  2014cited by this paper
• Dye-attached cryogels for reversible alcohol dehydrogenase immobilization.

  2014cited by this paper
• Aptamer binding assays for proteins: the thrombin example--a review.

  2014cited by this paper
• Affinity selection of histidine-containing peptides using metal chelate methacrylate monolithic disk for targeted LC-MS/MS approach in high-throughput proteomics.

  2014cited by this paper
• Aptamer-functionalized solid phase microextraction-liquid chromatography/tandem mass spectrometry for selective enrichment and determination of thrombin.

  2014cited by this paper
• A chitosan coated monolith for nucleic acid capture in a thermoplastic microfluidic chip.

  2014cited by this paper
• Human IgG adsorption using dye-ligand epoxy chitosan/alginate as adsorbent: influence of buffer system

  2014cited by this paper
• Boronate affinity monolith with a gold nanoparticle-modified hydrophilic polymer as a matrix for the highly specific capture of glycoproteins.

  2014cited by this paper
• Separation of human immunoglobulin G subclasses on a protein A monolith column.

  2014cited by this paper
• Versatile ene-thiol photoclick reaction for preparation of multimodal monolithic silica capillary columns.

  2014cited by this paper
• Biotin-functionalized poly(ethylene terephthalate) capillary-channeled polymer fibers as HPLC stationary phase for affinity chromatography

  2014cited by this paper
• Applications of monolithic materials for sample preparation.

  2014cited by this paper
• ptimization of supercoiled HPV-16 E 6 / E 7 plasmid DNA purification ith arginine monolith using design of experiments

  2014cited by this paper
• Hybrid Monoliths for Magnetically‐Driven Protein Separations

  2014cited by this paper
• Aptamer-based-sorbents for sample treatment—a review

  2014cited by this paper
• Affinity monolith chromatography: a review of principles and recent analytical applications

  2013cited by this paper
• Development of β-cyclodextrin-Modified Silica and Polyporous Polymer Particles for Solid-Phase Extraction of Methyl Jasmonate in Aqueous and Plant Samples

  2013cited by this paper
• Nanoparticle-modified monolithic pipette tips for phosphopeptide enrichment

  2013cited by this paper
• Identification of proteins interacting with ammodytoxins in Vipera ammodytes ammodytes venom by immuno-affinity chromatography

  2013cited by this paper
• Robust aptamer sol-gel solid phase microextraction of very polar adenosine from human plasma.

  2013cited by this paper
• Purification of human papillomavirus 16 E6/E7 plasmid deoxyribonucleic acid-based vaccine using an arginine modified monolithic support.

  2013cited by this paper
• Methacrylate monolithic columns functionalized with epinephrine for capillary electrochromatography applications.

  2013cited by this paper
• Preparation and characterization of DNA aptamer based spin column for enrichment and separation of histones.

  2012cited by this paper
• Monolithic columns with immobilized monomeric avidin: preparation and application for affinity chromatography

  2012cited by this paper
• Preparation of porous polymer monoliths featuring enhanced surface coverage with gold nanoparticles.

  2012cited by this paper

• Chromatography media and purification processes for complex and super-large biomolecules: A review.

  2025cites this paper
• Advances in Bioanalytical Techniques for Toxicological Applications: Emerging Techniques, Challenges, and Future Directions.

  2025cites this paper
• Polyhedral oligomeric silsesquioxane hybrid monolithic microcartridges for the determination of β-lactoglobulin in food by on-line solid-phase extraction capillary electrophoresis-mass spectrometry

  2025cites this paper
• Graphene Oxide-Activated Cellular Ceramic Composite Monoliths for Protein Purification

  2025cites this paper
• Expanding the applicability of biocompatible Salivette® as extraction sorbent in pipette-tip format: Determination of memantine in human urine as proof-of-concept.

  2025cites this paper
• Recent Advancements in Metal–Organic Framework-Based Microfluidic Chips for Biomedical Applications

  2025cites this paper
• Development of aptamer-based monolithic sorbent in paper devices for lysozyme extraction in foods.

  2025cites this paper
• 3D Printed Microfluidic Devices for Integrated Immunoaffinity Extraction, Solid-Phase Extraction, and Fluorescent Labeling of Preterm Birth Biomarkers

  2025cites this paper
• Cryogels and Monoliths: Promising Tools for Chromatographic Purification of Nucleic Acids

  2024cites this paper
• A INNOVATE APPROACH TO METAL REMOVAL IN CRYOGELS USING ELECTRICAL POTENTIAL IN A FIXED BED SYSTEM

  2024cites this paper
• Recent advances in zearalenone clean-up approaches from polluted food and feed: A mini-review of the State-of-the-art developments

  2024cites this paper
• Next-generation smart biomaterials for storage and extraction of bioanalytical samples: Current standing and path forward

  2024cites this paper
• Aptamer based hybrid monolithic pipette tips supported by melamine sponge for enrichment of proteins.

  2024cites this paper
• Microfluidic Capture Device for Simple, Cell Surface Marker-Based Quantification of Senescent CD8+ T Cells

  2024cites this paper
• Molecular Separation by Using Active and Passive Microfluidic chip Designs: A Comprehensive Review

  2023cites this paper
• Itaconic Acid-Based Organic-Polymer Monolithic Column for Hydrophilic Capillary Electrochromatography and Its Application in Pharmaceutical Analysis

  2023cites this paper
• Materials, workflows and applications of IMAC for phosphoproteome profiling in the recent decade: A review

  2023cites this paper
• Preparation of miniaturized hydrophilic affinity monoliths: Towards a reduction of non-specific interactions and an increased target protein density.

  2022cites this paper
• Current developments of bioanalytical sample preparation techniques in pharmaceuticals

  2022cites this paper
• Preparation of Affinity Chromatography Monolith in Miniaturized Format and Application for Protein Separation.

  2022cites this paper
• Matrices and Affinity Ligands for Antibody Purification and Corresponding Applications in Radiotherapy

  2022cites this paper
• A high-throughput magnetic-based pipette tip microextraction as an alternative to conventional pipette tip strategies: Determination of testosterone in human saliva as a proof-of-concept.

  2022cites this paper
• Preparation of Affinity Monolithic Column for -amylase Separation

  2022cites this paper
• Analysis of carbohydrates and glycoconjugates by matrix-assisted laser desorption/ionization mass spectrometry: An update for 2019-2020.

  2022cites this paper
• Advances and applications of in-tube solid-phase microextraction for analysis of proteins.

  2021cites this paper
• Selection and characterization of DNA aptamers for highly selective recognition of the major allergen of olive pollen Ole e 1.

  2021cites this paper
• Monolithic solids: synthesis and uses in microextraction techniques

  2021cites this paper
• In situ photo-initiated polymerized oligonucleotide-functionalized hydrophilic capillary affinity monolith for highly selective in-tube microextraction of ochratoxin A mycotoxin

  2021cites this paper
• Affinity monolith chromatography: A review of general principles and recent developments

  2021cites this paper
• Extraction and determination of tussilagone from Farfarae Flos with online solid-phase extraction-HPLC using a homemade monolithic cartridge doped with porous organic cage material.

  2021cites this paper
• Facile DNA adsorption enabling ammonium-based hydrophilic affinity monolithic column for high-performance online selective microextraction of ochratoxin A.

  2021cites this paper
• Modern Microfluidic Approaches for Detection and Quantitation of Ions

  2021cites this paper
• Solid phase extraction based microfluidic chip coupled with mass spectrometry for rapid determination of aflatoxins in peanut oil

  2021cites this paper
• Smart materials for sample preparation in bioanalysis: A green overview

  2021cites this paper
• Recent advances in aptamer-based miniaturized extraction approaches in food analysis

  2021cites this paper
• Macroporous Polymer Monoliths in Thin Layer Format

  2021cites this paper
• A poly(N,N-dimethylaminoethyl methacrylate-co-ethylene glycol dimethacrylate) monolith for direct solid-phase extraction of benzodiazepines from undiluted human urine.

  2020cites this paper
• Preparation, characterization and evaluation of hydrophilic polymers containing magnetic nanoparticles and amine-modified carbon nanotubes for the determination of anti-inflammatory drugs in urine samples.

  2020cites this paper
• Facile fabrication of functional groups-rich sorbent for the efficient enrichment of aromatic N- and S-containing compounds in environmental waters.

  2020cites this paper
• Extraction of β-blockers from urine with a polymeric monolith modified with 1-allyl-3-methylimidazolium chloride in spin column format.

  2020cites this paper
• Recent trends in sorbents for bioaffinity chromatography.

  2020cites this paper
• Microfluidic strategies for extraction and preconcentration of proteins and peptides

  2020cites this paper
• Redefining the chemistry of super-macroporous materials: when dendritic molecules meet polymer cryogels

  2020cites this paper
• A robust affinity chromatography system based on ceramic monoliths coated with poly(amino acid)‐based polymeric constructs

  2020cites this paper
• Design and regulation of the surface and interfacial behavior of protein molecules

  2020cites this paper
• Highly permeable monolith-based multichannel in-tip microextraction apparatus for simultaneous field sample preparation of pesticides and heavy metal ions in environmental waters.

  2019cites this paper
• Fabrication of Piperazine Functionalized Polymeric Monolithic Tip for Rapid Enrichment of Glycopeptides/Glycans.

  2019cites this paper