Closer to Nature Through Dynamic Culture Systems

Mechanics in the human body are required for normal cell function at a molecular level. It is now clear that mechanical stimulations play significant roles in cell growth, differentiation, and migration in normal and diseased cells. Recent studies have led to the discovery that normal and cancer cells have different mechanosensing properties. Here, we discuss the application and the physiological and pathological meaning of mechanical stimulations. To reveal the optimal conditions for mimicking an in vivo microenvironment, we must, therefore, discern the mechanotransduction occurring in cells.

• Publication year

  2019

• Venue

  Cells

• Publication date

  2019-08-21

• Fields of study

  Biology, Medicine, Engineering

• Identifiers
  DOI 10.3390/cells8090942PMID 31438519PMCID 6769584
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Reconstruction of structure and function in tissue engineering of solid organs: Toward simulation of natural development based on decellularization

  2018cited by this paper
• Chemical cocktails enable hepatic reprogramming of human urine-derived cells with a single transcription factor

  2018cited by this paper
• Mechanical Stretching Simulates Cardiac Physiology and Pathology through Mechanosensor Piezo1

  2018cited by this paper
• Src kinase inhibitor PP2 regulates the biological characteristics of A549 cells via the PI3K/Akt signaling pathway

  2018cited by this paper
• Modeling Human Cardiac Hypertrophy in Stem Cell-Derived Cardiomyocytes

  2018cited by this paper
• Mechanotransduction in tumor progression: The dark side of the force

  2018cited by this paper
• Yoda1‐induced phosphorylation of the Akt and ERK1/2 does not require Piezo1 activation

  2018cited by this paper
• Mechanical forces in skin disorders.

  2018cited by this paper
• Fructose fuels lung adenocarcinoma through GLUT5

  2018cited by this paper
• Emerging views of the nucleus as a cellular mechanosensor

  2018cited by this paper
• Mechanical regulation of stem cell differentiation through stretch-activated Piezo channel

  2018cited by this paper
• Role of connexin 43 in different forms of intercellular communication – gap junctions, extracellular vesicles and tunnelling nanotubes

  2017cited by this paper
• Mechanical regulation of cardiac fibroblast profibrotic phenotypes

  2017cited by this paper
• The roles and regulation of the actin cytoskeleton, intermediate filaments and microtubules in smooth muscle cell migration

  2017cited by this paper
• Receptor-mediated cell mechanosensing

  2017cited by this paper
• Chemokines protect vascular smooth muscle cells from cell death induced by cyclic mechanical stretch

  2017cited by this paper
• Roles of the cytoskeleton, cell adhesion and rho signalling in mechanosensing and mechanotransduction.

  2017cited by this paper
• NAOSITE : Nagasaki University ' s Academic Output SITE

  2017cited by this paper
• Tissue mechanics regulate brain development, homeostasis and disease

  2017cited by this paper
• Emerging roles of mechanical forces in chromatin regulation

  2017cited by this paper
• EGFR and HER2 Activate Rigidity Sensing Only on Rigid Matrices

  2017cited by this paper
• Mechanisms of Vascular Smooth Muscle Contraction and the Basis for Pharmacologic Treatment of Smooth Muscle Disorders

  2016cited by this paper
• Electrical and mechanical stimulation of cardiac cells and tissue constructs.

  2016cited by this paper
• Mechanical Stress Conditioning and Electrical Stimulation Promote Contractility and Force Maturation of Induced Pluripotent Stem Cell-Derived Human Cardiac Tissue

  2016cited by this paper
• The Cell Nucleus Serves as a Mechanotransducer of Tissue Damage-Induced Inflammation.

  2016cited by this paper
• Piezo2 senses airway stretch and mediates lung inflation-induced apnoea

  2016cited by this paper
• The Effect of Substrate Stiffness on Cardiomyocyte Action Potentials

  2016cited by this paper
• Chemical and mechanical stimuli act on common signal transduction and cytoskeletal networks

  2016cited by this paper
• Influence of body weight on bone mass, architecture and turnover.

  2016cited by this paper
• A potent and selective small molecule inhibitor of sirtuin 1 promotes differentiation of pluripotent P19 cells into functional neurons

  2016cited by this paper
• Ventilator-induced Lung Injury.

  2016cited by this paper
• Mechanosensing Controlled Directly by Tyrosine Kinases.

  2016cited by this paper
• Mechanotransduction in cancer.

  2016cited by this paper
• Biomechanics of vascular mechanosensation and remodeling

  2016influential reference
• Biologic-free mechanically induced muscle regeneration

  2016cited by this paper
• Cyclic stretch and compression forces alter microRNA-29 expression of human periodontal ligament cells.

  2015cited by this paper
• Piezo1 links mechanical forces to red blood cell volume

  2015cited by this paper
• Interactions between Hyaluronan and Its Receptors (CD44, RHAMM) Regulate the Activities of Inflammation and Cancer

  2015cited by this paper
• Static stretch affects neural stem cell differentiation in an extracellular matrix-dependent manner

  2015cited by this paper
• Molecular mechanism of extrinsic factors affecting anti-aging of stem cells.

  2015cited by this paper
• Molecular and Mechanical Causes of Microtubule Catastrophe and Aging.

  2015cited by this paper
• Caveolae protect endothelial cells from membrane rupture during increased cardiac output

  2015cited by this paper
• Cyclic stretch induced gene expression of extracellular matrix and adhesion molecules in human periodontal ligament cells.

  2015cited by this paper
• Effects of cyclic tension stress on the apoptosis of osteoclasts in vitro.

  2015cited by this paper
• Calcium channel blockade reduces mechanical strain-induced extracellular matrix gene response in lamina cribrosa cells

  2015cited by this paper
• Piezo1 in Smooth Muscle Cells Is Involved in Hypertension-Dependent Arterial Remodeling.

  2015cited by this paper
• Stretch and/or oxygen glucose deprivation (OGD) in an in vitro traumatic brain injury (TBI) model induces calcium alteration and inflammatory cascade

  2015cited by this paper
• CD44, Hyaluronan, the Hematopoietic Stem Cell, and Leukemia-Initiating Cells

  2015cited by this paper
• Cyclic stretch and hypertension increase retinal succinate: potential mechanisms for exacerbation of ocular neovascularization by mechanical stress.

  2014cited by this paper
• Cyclic Strain-Induced Cytoskeletal Rearrangement of Human Periodontal Ligament Cells via the Rho Signaling Pathway

  2014cited by this paper
• The role of integrin-β/FAK in cyclic mechanical stimulation in MG-63 cells.

  2014cited by this paper
• Matrix metalloproteinase (MMP)‐2 and MMP‐9 as inflammation markers of Trichinella spiralis and Trichinella pseudospiralis infections in mice

  2014cited by this paper
• Fibroblasts in fibrosis: novel roles and mediators

  2014cited by this paper
• Self-assembled pH-responsive hyaluronic acid-g-poly((L)-histidine) copolymer micelles for targeted intracellular delivery of doxorubicin.

  2014cited by this paper
• Microenvironmental Stiffness Enhances Glioma Cell Proliferation by Stimulating Epidermal Growth Factor Receptor Signaling

  2014cited by this paper
• Uniaxial Cyclic Stretch Promotes Osteogenic Differentiation and Synthesis of BMP2 in the C3H10T1/2 Cells with BMP2 Gene Variant of rs2273073 (T/G)

  2014cited by this paper
• Matrix elasticity regulates the optimal cardiac myocyte shape for contractility.

  2014cited by this paper
• Mechanosensitive release of adenosine 5′‐triphosphate through pannexin channels and mechanosensitive upregulation of pannexin channels in optic nerve head astrocytes: A mechanism for purinergic involvement in chronic strain

  2014cited by this paper
• Prestress in the extracellular matrix sensitizes latent TGF-β1 for activation

  2014cited by this paper
• Down-regulated expression of vimentin induced by mechanical stress in fibroblasts derived from patients with ossification of the posterior longitudinal ligament

  2014cited by this paper
• The Expression of Fn14 via Mechanical Stress-activated JNK Contributes to Apoptosis Induction in Osteoblasts*

  2014cited by this paper
• Expression of MMP-2, MT1-MMP, and TIMP-2 by cultured rabbit corneal fibroblasts under mechanical stretch

  2014cited by this paper
• Structure and function of μ-conotoxins, peptide-based sodium channel blockers with analgesic activity.

  2014cited by this paper
• Mechanical checkpoint for persistent cell polarization in adhesion-naive fibroblasts.

  2014cited by this paper
• Feeling the hidden mechanical forces in lipid bilayer is an original sense

  2014cited by this paper
• Measurement Science in the Circulatory System

  2014cited by this paper
• A close look at the contraction and relaxation of the myometrium; the role of calcium.

  2013cited by this paper
• Increased proliferation of human bladder smooth muscle cells is mediated by physiological cyclic stretch via the PI3K‑SGK1‑Kv1.3 pathway.

  2013cited by this paper
• Death receptor-ligand systems in cancer, cell death, and inflammation.

  2013cited by this paper
• Mechanical Stretch Inhibits Lipopolysaccharide-induced Keratinocyte-derived Chemokine and Tissue Factor Expression While Increasing Procoagulant Activity in Murine Lung Epithelial Cells*

  2013cited by this paper
• Cyclical stretch induces structural changes in atrial myocytes

  2013cited by this paper
• The TREK2 Channel Is Involved in the Proliferation of 253J Cell, a Human Bladder Carcinoma Cell

  2013cited by this paper
• Physiological cyclic strain promotes endothelial cell survival via the induction of heme oxygenase-1.

  2013cited by this paper
• Measuring nonequilibrium vesicle dynamics in neurons under tension.

  2013cited by this paper
• Pathological Cyclic Strain-Induced Apoptosis in Human Periodontal Ligament Cells through the RhoGDIα/Caspase-3/PARP Pathway

  2013cited by this paper
• Experimental study on the mechanical strain of corneal collagen.

  2013cited by this paper
• Mechanically Loaded Myotubes Affect Osteoclast Formation

  2013cited by this paper
• Cyclic Stretch Induces Inducible Nitric Oxide Synthase and Soluble Guanylate Cyclase in Pulmonary Artery Smooth Muscle Cells

  2013cited by this paper
• A tissue-engineered jellyfish with biomimetic propulsion

  2012cited by this paper
• Mitochondrial Injury after Mechanical Stretch of Cortical Neurons in vitro: Biomarkers of Apoptosis and Selective Peroxidation of Anionic Phospholipids

  2012cited by this paper
• APJ ACTS AS A DUAL RECEPTOR IN CARDIAC HYPERTROPHY

  2012cited by this paper
• The role of ventilation-induced surfactant dysfunction and atelectasis in causing acute respiratory distress syndrome.

  2012cited by this paper
• Shape change of the vitreous chamber influences retinal detachment and reattachment processes: is mechanical stress during eye rotations a factor?

  2012cited by this paper
• Improved Outcome after Peripheral Nerve Injury in Mice with Increased Levels of Endogenous Omega-3 Polyunsaturated Fatty Acids

  2012cited by this paper
• The effect of cyclic mechanical strain on the expression of adhesion-related genes by periodontal ligament cells in two-dimensional culture.

  2012cited by this paper
• Hyaluronan Regulates Cell Behavior: A Potential Niche Matrix for Stem Cells

  2012cited by this paper
• MicroRNA modulate alveolar epithelial response to cyclic stretch

  2012cited by this paper
• At the leading edge of three-dimensional cell migration

  2012cited by this paper
• Mechanical stretch induces the apoptosis regulator PUMA in vascular smooth muscle cells.

  2012cited by this paper
• Allosteric Modulation of Beta1 Integrin Function Induces Lung Tissue Repair

  2012cited by this paper
• Control of Vascular Smooth Muscle Cell Growth by Connexin 43

  2012cited by this paper
• Uses of Remnant Human Lung Tissue for Mechanical Stretch Studies

  2012cited by this paper
• Cells test substrate rigidity by local contractions on submicrometer pillars

  2012cited by this paper
• Infarcted myocardium-like stiffness contributes to endothelial progenitor lineage commitment of bone marrow mononuclear cells

  2011cited by this paper
• Mechanical compression drives cancer cells toward invasive phenotype

  2011cited by this paper
• Growth of Engineered Human Myocardium With Mechanical Loading and Vascular Coculture

  2011cited by this paper
• Mitotic Spindle Orients Perpendicular to the Forces Imposed by Dynamic Shear

  2011cited by this paper
• Mechanical Stretch Induces Epithelial-Mesenchymal Transition in Alveolar Epithelia via Hyaluronan Activation of Innate Immunity*

  2011cited by this paper
• Alterations in expression of HIF-1α, HIF-2α, and VEGF by idiopathic overactive bladder urothelial cells during stretch suggest role for hypoxia.

  2011cited by this paper
• Fibroblast polarization is a matrix-rigidity-dependent process controlled by focal adhesion mechanosensing

  2011cited by this paper

• Advances in lung biomimetic systems: exploring biophysical cues in lung regenerative medicine.

  2025cites this paper
• Biomineralization empowers bone organoids.

  2025cites this paper
• Current challenges and future directions of ATMPs in regenerative medicine

  2025cites this paper
• Organ-On-A-Chip (OOC) Image Dataset for Machine Learning and Tissue Model Evaluation

  2024cites this paper
• Simulating big mechanically-active culture systems (BigMACS) using paired biomechanics-histology FEA modelling to derive mechanobiology design relationships

  2024cites this paper
• In Vitro Spermatogenesis on Human Decellularized Testicular Matrix Plates Following Exosome Treatment in a Dynamic Culture System

  2024cites this paper
• Modularized dynamic cell culture platform for efficient production of extracellular vesicles and sequential analysis.

  2023cites this paper
• The use of paracetamol during pregnancy: A qualitative study and possible strategies for a clinical trial

  2022cites this paper
• Physiological Shear Stress Enhances Differentiation, Mucus-Formation and Structural 3D Organization of Intestinal Epithelial Cells In Vitro

  2021cites this paper
• Fabrication Approaches for High-Throughput and Biomimetic Disease Modeling.

  2021cites this paper
• Fabrication of Adhesive Substrate for Incorporating Hydrogels to Investigate the Influence of Stiffness on Cancer Cell Behavior.

  2021cites this paper
• The effects of oscillatory temperature on HaCaT keratinocyte behaviors

  2020cites this paper
• Cancer Cell Signaling: Methods and Protocols

  2018cites this paper
• Src kinase inhibitor PP2 regulates the biological characteristics of A549 cells via the PI3K/Akt signaling pathway

  2018cites this paper