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Two B-box proteins, PpBBX18 and PpBBX21, antagonistically regulate anthocyanin biosynthesis via competitive association with PpHY5 in the peel of pear fruit.

S. Bai,Ruiyan Tao,Lei Yin,J. Ni,Qinsong Yang,Xinhui Yan,F. Yang,Xianping Guo,Hongxu Li,Yuanwen Teng

Published 2019 in The Plant Journal

ABSTRACT

Light is indispensable for anthocyanin accumulation in the peel of red pear fruit (Pyrus pyrifolia Nakai). HY5 is considered a critical regulator for induction of anthocyanin biosynthesis, but detailed characterization of its regulatory mechanism is needed. In this study, multiple genetic and biochemical approaches were applied to identify the roles of PpHY5 and two BBX proteins, PpBBX18 and PpBBX21, in the transcriptional regulation of PpMYB10. The functions of the two BBX proteins were analyzed in overexpression lines using pear calli-based approaches. PpHY5 alone was unable to activate downstream genes. The two BBX proteins, PpBBX18 and PpBBX21, physically interacted with PpHY5 and antagonistically regulated anthocyanin biosynthesis in Arabidopsis and pear. PpBBX18 formed a heterodimer with PpHY5 via two B-box domains, in which PpHY5 bound to the G-box motif of PpMYB10 and PpBBX18 provided the trans-acting activity, thus inducing transcription of PpMYB10. PpBBX21 interacted with PpHY5 and PpBBX18, and hampered formation of the PpHY5-PpBBX18 active transcription activator complex and subsequently repressed anthocyanin biosynthesis. The present results demonstrate the fine-tuned regulation of anthocyanin biosynthesis via transcriptional regulation of PpMYB10 by PpHY5-associated proteins and provide insights into light-induced anthocyanin biosynthesis. This article is protected by copyright. All rights reserved.

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