Induction of apoptosis in HeLa cells by chloroform fraction of seed extracts of Nigella sativa

BackgroundCancer remains one of the most dreaded diseases causing an astonishingly high death rate, second only to cardiac arrest. The fact that conventional and newly emerging treatment procedures like chemotherapy, catalytic therapy, photodynamic therapy and radiotherapy have not succeeded in reverting the outcome of the disease to any drastic extent, has made researchers investigate alternative treatment options. The extensive repertoire of traditional medicinal knowledge systems from various parts of the world are being re-investigated for their healing properties. This study progresses in the direction of identifying component(s) from Nigella sativa with anti cancer acitivity. In the present study we investigated the efficacy of Organic extracts of Nigella sativa seed powder for its clonogenic inhibition and induction of apoptosis in HeLa cancer cell.ResultsMethanolic, n-Hexane and chloroform extracts of Nigella sativa seedz effectively killed HeLa cells. The IC50 values of methanolic, n-hexane, and chloroform extracts of Nigella sativa were 2.28 μg/ml, 2.20 μg/ml and 0.41 ng/ml, respectively. All three extracts induced apoptosis in HeLa cells. Apoptosis was confirmed by DNA fragmentation, western blot and terminal transferase-mediated dUTP-digoxigenin-end labeling (TUNEL) assay.ConclusionWestern Blot and TUNEL results suggested that Nigella sativa seed extracts regulated the expression of pro- and anti- apoptotic genes, indicating its possible development as a potential therapeutic agent for cervical cancer upon further investigation.

• Publication year

  2009

• Venue

  Cancer Cell International

• Publication date

  2009-11-27

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1186/1475-2867-9-29PMID 19943925PMCID 2794855
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• Nigella sativa seed extracts regulate the expression of pro- and anti-apoptotic genes.

  Confidence 0.90

  All you need is Python (5d7gwfm5zu) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• All three Nigella sativa seed extract fractions induce apoptosis in HeLa cells, as confirmed by DNA fragmentation, Western blot, and TUNEL assay.

  Confidence 0.95

  All you need is Python (5d7gwfm5zu) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• Methanolic, n-Hexane, and chloroform extracts of Nigella sativa seeds effectively kill HeLa cells with IC50 values of 2.28 μg/ml, 2.20 μg/ml, and 0.41 ng/ml, respectively.

  Confidence 0.95

  All you need is Python (5d7gwfm5zu) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review

• apoptosis

  biological process

  Programmed cell death evaluated as a mechanism of cell killing in this study.

  All you need is Python (5d7gwfm5zu) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• hela cells

  cell line

  A human cervical cancer cell line used as the experimental model.

  Aliases: HeLa cancer cell

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• ic50

  measurement

  The half-maximal inhibitory concentration metric used to quantify extract potency.

  Aliases: IC50 values

  All you need is Python (5d7gwfm5zu) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• nigella sativa seed extracts

  substance

  Organic extracts (methanolic, n-hexane, and chloroform fractions) derived from Nigella sativa seeds tested for anti-cancer activity.

  Aliases: Nigella sativa, seed extracts of Nigella sativa

  All you need is Python (5d7gwfm5zu) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• pro- and anti-apoptotic genes

  gene group

  Genes that control the apoptotic pathway, evaluated for expression changes via Western blot.

  All you need is Python (5d7gwfm5zu) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review
• tunel assay

  assay

  Terminal transferase-mediated dUTP-digoxigenin-end labeling assay used to detect apoptotic DNA fragmentation.

  Aliases: terminal transferase-mediated dUTP-digoxigenin-end labeling

  All you need is Python (5d7gwfm5zu) extraction뀨 (7c402c1b98) review--------- ✂ Cut Here ✂ --------- (jqthcshryb) review

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