Examination of the literature for hprt mutant frequencies from peripheral T cells yielded data from 1194 human subjects. Relationships between mutant frequency, age, sex, and smoking were examined, and the kinetics were described. Mutant frequency increases rapidly with age until about age 15. Afterward, the rate of increase falls such that after age 53, the hprt mutant frequency is largely stabilized. Sex had no effect on mutant frequency. Cigarette smoking increased mean mutant frequency compared to nonsmokers, but did not alter age vs. mutant frequency relationships. An hprt in vivo mutant database containing 795 human hprt mutants from 342 individuals was prepared. No difference in mutational spectra was observed comparing smokers to nonsmokers, confirming previous reports. Sex affected the frequency of deletions (>1 bp) that are recovered more than twice as frequently in females (P = 0. 008) compared to males. There is no indication of a significant shift in mutational spectra with age for individuals older than 19 yr, with the exception of A:T --> C:G transversions. These events are recovered more frequently in older individuals.
Influence of sex, smoking and age on human hprt mutation frequencies and spectra.
John Curry,Larissa Karnaoukhova,Gabriel C. Guenette,Barry W. Glickman
Published 1999 in Genetics
ABSTRACT
PUBLICATION RECORD
- Publication year
1999
- Venue
Genetics
- Publication date
1999-07-01
- Fields of study
Biology, Medicine
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- age
The subject age variable analyzed for its relationship to HPRT mutation frequency and spectra.
Aliases: older individuals, years of age
- a:t -> c:g transversions
A base-substitution mutation class tracked among HPRT variants in the age-stratified analysis.
Aliases: A:T to C:G transversions, AT to CG transversions
- cigarette smoking
Smoking status of subjects, used to compare mutation frequency and spectra between smokers and nonsmokers.
Aliases: smoking, smoker status
- deletions (>1 bp)
Deletion mutations larger than one base pair among the recovered HPRT mutants.
Aliases: large deletions, deletions greater than 1 bp
- hprt in vivo mutant database
The compiled human in vivo HPRT mutant collection summarized in the abstract, including mutants and donor counts.
- hprt mutant frequency
The measured frequency of mutations at the human HPRT locus in peripheral T-cell samples.
Aliases: mutant frequency
- hprt mutant spectra
The distribution of mutation classes observed among human HPRT mutants in the database.
Aliases: HPRT mutational spectra, mutational spectra
- peripheral t cells
Blood T lymphocytes used as the source tissue for measuring HPRT mutations in this dataset.
Aliases: peripheral blood T cells, T cells
- sex
The male-versus-female subject attribute examined for differences in HPRT mutation measures.
Aliases: gender
REFERENCES
CITED BY
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