CRISPR/Cas9: An inexpensive, efficient loss of function tool to screen human disease genes in Xenopus.

No abstract is available for this paper.

• Publication year

  2015

• Venue

  Developmental Biology

• Publication date

  2015-12-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.ydbio.2015.11.003PMID 26546975PMCID PMC4684459
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Fast and sensitive detection of indels induced by precise gene targeting

  2015cited by this paper
• Global genetic analysis in mice unveils central role for cilia in congenital heart disease

  2015cited by this paper
• Efficient Multiple Genome Modifications Induced by the crRNAs, tracrRNA and Cas9 Protein Complex in Zebrafish

  2015cited by this paper
• Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish.

  2015cited by this paper
• CRISPR/Cas9 mediated genome engineering in Drosophila.

  2014cited by this paper
• Cas9-based genome editing in Xenopus tropicalis.

  2014cited by this paper
• Efficient RNA/Cas9-mediated genome editing in Xenopus tropicalis

  2014influential reference
• Highly efficient gene knockout in mice and zebrafish with RNA-guided endonucleases

  2014cited by this paper
• Increased Frequency of De Novo Copy Number Variants in Congenital Heart Disease by Integrative Analysis of Single Nucleotide Polymorphism Array and Exome Sequence Data

  2014cited by this paper
• Efficient Mutagenesis by Cas9 Protein-Mediated Oligonucleotide Insertion and Large-Scale Assessment of Single-Guide RNAs

  2014cited by this paper
• Simple and efficient CRISPR/Cas9‐mediated targeted mutagenesis in Xenopus tropicalis

  2013influential reference
• Genetic Screens in Human Cells Using the CRISPR-Cas9 System

  2013cited by this paper
• High‐resolution analysis of copy number variants in adults with simple‐to‐moderate congenital heart disease

  2013influential reference
• Heritable genome editing in C. elegans via a CRISPR-Cas9 system

  2013cited by this paper
• The Heterotaxy gene, GALNT11, glycosylates Notch to orchestrate cilia type and laterality

  2013influential reference
• The Congenital Heart Disease Genetic Network Study: Rationale, Design, and Early Results

  2013cited by this paper
• Biallelic genome modification in F0 Xenopus tropicalis embryos using the CRISPR/Cas system

  2013influential reference
• De novo mutations in histone modifying genes in congenital heart disease

  2013cited by this paper
• Heritable gene targeting in the mouse and rat using a CRISPR-Cas system

  2013cited by this paper
• Efficient In Vivo Genome Editing Using RNA-Guided Nucleases

  2013cited by this paper
• The contribution of de novo and rare inherited copy number changes to congenital heart disease in an unselected sample of children with conotruncal defects or hypoplastic left heart disease

  2013influential reference
• Rare Copy Number Variants Contribute to Congenital Left-Sided Heart Disease

  2012cited by this paper
• Rare Copy Number Variants in Isolated Sporadic and Syndromic Atrioventricular Septal Defects

  2012influential reference
• targeted gene knockout by direct delivery of zinc-finger nuclease proteins

  2012cited by this paper
• Targeted gene knockout by direct delivery of ZFN proteins

  2012cited by this paper
• Exon capture and bulk segregant analysis: rapid discovery of causative mutations using high-throughput sequencing

  2012cited by this paper
• Rare copy number variations in congenital heart disease patients identify unique genes in left-right patterning

  2011cited by this paper
• Efficient gene targeting mediated by a lentiviral vector-associated meganuclease

  2011cited by this paper
• Birth prevalence of congenital heart disease worldwide: a systematic review and meta-analysis.

  2011cited by this paper
• The coiled-coil domain containing protein CCDC40 is essential for motile cilia function and left-right axis formation

  2011cited by this paper
• Congenital Heart Defects in Europe Prevalence and Perinatal Mortality, 2000 to 2005

  2011cited by this paper
• Congenital Heart Defects in Europe: Prevalence and Perinatal Mortality, 2000 to 2005

  2011cited by this paper
• Supplements

  2009influential reference
• Disorders of left–right asymmetry: Heterotaxy and situs inversus

  2009cited by this paper
• Flow on the right side of the gastrocoel roof plate is dispensable for symmetry breakage in the frog Xenopus laevis.

  2009cited by this paper
• De Novo Copy Number Variants Identify New Genes and Loci in Isolated, Sporadic Tetralogy of Fallot

  2009cited by this paper
• The Forkhead protein, FoxJ1, specifies node-like cilia in Xenopus and Zebrafish embryos

  2008influential reference
• Heterotaxia, congenital heart disease, and primary ciliary dyskinesia.

  2007cited by this paper
• Depletion of three BMP antagonists from Spemann's organizer leads to a catastrophic loss of dorsal structures.

  2005cited by this paper
• Techniques and probes for the study of Xenopus tropicalis development

  2002influential reference
• An economic method for the fluorescent labeling of PCR fragments

  2000cited by this paper
• Beta-catenin signaling activity dissected in the early Xenopus embryo: a novel antisense approach.

  2000influential reference
• Mutation of an axonemal dynein affects left–right asymmetry in inversus viscerum mice

  1997cited by this paper
• Maternal beta-catenin establishes a 'dorsal signal' in early Xenopus embryos.

  1996cited by this paper
• Overexpression of cadherins and underexpression of beta-catenin inhibit dorsal mesoderm induction in early Xenopus embryos.

  1994cited by this paper
• Overexpression of cadherins and underexpression of β-catenin inhibit dorsal mesoderm induction in early Xenopus embryos

  1994cited by this paper
• Monoclonal antibodies specific for an acetylated form of alpha-tubulin recognize the antigen in cilia and flagella from a variety of organisms

  1985cited by this paper

• Novel centriolar defects underlie a primary ciliary dyskinesia phenotype in an adenylate kinase 7 deficient ciliated epithelium.

  2025cites this paper
• X-CRISP: Domain-Adaptable and Interpretable CRISPR Repair Outcome Prediction

  2025cites this paper
• The Xenopus Oocyte System: Molecular Dynamics of Maturation, Fertilization, and Post-Ovulatory Fate

  2025cites this paper
• Autism gene variants disrupt enteric neuron migration and cause gastrointestinal dysmotility

  2025cites this paper
• X-CRISP: domain-adaptable and interpretable CRISPR repair outcome prediction

  2025cites this paper
• Genomic Complexity of ccdc40 in Xenopus : Implications for CRISPR Targeting and Disease Modeling

  2025influential citation
• Phenotype to genotype: A new and rapid approach using whole-genome sequencing

  2025cites this paper
• Xenopus laevis as an infection model for human pathogenic bacteria

  2025cites this paper
• AP2A2 mutation and defective endocytosis in a Malian family with hereditary spastic paraplegia.

  2024influential citation
• CC2D1A causes ciliopathy, intellectual disability, heterotaxy, renal dysplasia, and abnormal CSF flow

  2024cites this paper
• WheresWalker: a pipeline for rapid mutation mapping using whole genome sequencing

  2024cites this paper
• The Heterotaxy Gene CCDC11 Is Important for Cytokinesis via RhoA Regulation

  2024cites this paper
• Marcks and Marcks-like 1 proteins promote spinal cord development and regeneration in Xenopus

  2024cites this paper
• Xenopus as a model system for studying pigmentation and pigmentary disorders

  2024cites this paper
• Modelling human genetic disorders in Xenopus tropicalis

  2024cites this paper
• Diphthamide deficiency promotes association of eEF2 with p53 to induce p21 expression and neural crest defects

  2024cites this paper
• CFAP45, a heterotaxy and congenital heart disease gene, affects cilia stability

  2023cites this paper
• A novel SMARCC1-mutant BAFopathy implicates epigenetic dysregulation of neural progenitors in hydrocephalus

  2023cites this paper
• CRISPR/Cas9 Gene Disruption Studies in F0 Xenopus Tadpoles: Understanding Development and Disease in the Frog.

  2023cites this paper
• A novel SMARCC1 BAFopathy implicates neural progenitor epigenetic dysregulation in human hydrocephalus.

  2023cites this paper
• Genetic mechanisms of multiciliated cell development: from fate choice to differentiation in zebrafish and other models

  2023cites this paper
• Use of Frogs as a Model to Study the Etiology of HLHS

  2023cites this paper
• Mitochondrial leak metabolism induces the Spemann-Mangold Organizer via Hif-1α in Xenopus.

  2023cites this paper
• Phenotype–genotype relationships in Xenopus sox9 crispants provide insights into campomelic dysplasia and vertebrate jaw evolution

  2023cites this paper
• Novel centriolar defects underlie a primary ciliary dyskinesia phenotype in an adenylate kinase 7 deficient ciliated epithelium

  2023cites this paper
• Dual leucine zipper kinase is necessary for retinal ganglion cell axonal regeneration in Xenopus laevis

  2023cites this paper
• Identification and validation of candidate risk genes in endocytic vesicular trafficking associated with esophageal atresia and tracheoesophageal fistulas

  2022cites this paper
• Maternal Wnt11b regulates cortical rotation during Xenopus axis formation: analysis of maternal-effect wnt11b mutants

  2022cites this paper
• Myrf Represses Nodal Signaling Via Furin During Left-Right Patterning And Gastrulation

  2022influential citation
• Mink1 regulates spemann organizer cell fate in the xenopus gastrula via Hmga2.

  2022cites this paper
• Membrane potential drives the exit from pluripotency and cell fate commitment via calcium and mTOR

  2022cites this paper
• Delivering the CRISPR/Cas9 system for engineering gene therapies: Recent cargo and delivery approaches for clinical translation

  2022cites this paper
• Responsible governance of human germline genome editing in China

  2022cites this paper
• Expanding EMC Foldopathies: Topogenesis Deficits Alter the Neural Crest

  2022cites this paper
• Long Non Coding RNA Based Regulation of Cerebrovascular Endothelium

  2022cites this paper
• Xenopus to the rescue: A model to validate and characterize candidate ciliopathy genes

  2021cites this paper
• Aquatic models of human ciliary diseases

  2021cites this paper
• Parallel in vivo analysis of large-effect autism genes implicates cortical neurogenesis and estrogen in risk and resilience.

  2021cites this paper
• Modeling Human Genetic Disorders with CRISPR Technologies in Xenopus.

  2021cites this paper
• Tissue-Targeted CRISPR-Cas9-Mediated Genome Editing of Multiple Homeologs in F0-Generation Xenopus laevis Embryos.

  2021cites this paper
• CRISPR-Cas9 Mutagenesis in Xenopus tropicalis for Phenotypic Analyses in the F0 Generation and Beyond.

  2021cites this paper
• Mechanical stretch scales centriole number to apical area via Piezo1 in multiciliated cells

  2021cites this paper
• Xenopus as a platform for discovery of genes relevant to human disease.

  2021cites this paper
• Kap-β2/Transportin mediates β-catenin nuclear transport in Wnt signaling

  2021cites this paper
• Identification and validation of novel candidate risk genes in endocytic vesicular trafficking associated with esophageal atresia and tracheoesophageal fistulas

  2021cites this paper
• Anaplastic lymphoma kinase (alk), a neuroblastoma associated gene, is expressed in neural crest domains during embryonic development of Xenopus

  2021cites this paper
• Modeling endoderm development and disease in Xenopus.

  2021cites this paper
• Progression and application of CRISPR-Cas genomic editors.

  2021cites this paper
• Functional characterization of two 20β-hydroxysteroid dehydrogenase type 2 homeologs from Xenopus laevis reveals multispecificity.

  2021cites this paper
• Generation of a new six1-null line in Xenopus tropicalis for study of development and congenital disease

  2021cites this paper
• Developmental Neuroendocrinology

  2020cites this paper
• Xenopus Models of Organogenesis and Disease

  2020influential citation
• Chromatin accessibility dynamics and single cell RNA-Seq reveal new regulators of regeneration in neural progenitors

  2020cites this paper
• CPAMD8 loss-of-function underlies non-dominant congenital glaucoma with variable anterior segment dysgenesis and abnormal extracellular matrix

  2020cites this paper
• Xenopus leads the way: Frogs as a pioneering model to understand the human brain

  2020cites this paper
• DAAM2 Variants Cause Nephrotic Syndrome via Actin Dysregulation.

  2020cites this paper
• Xenopus in revealing developmental toxicity and modeling human diseases.

  2020cites this paper
• CRISPR-Cas9: A Preclinical and Clinical Perspective for the Treatment of Human Diseases.

  2020cites this paper
• Nucleoporin NUP205 plays a critical role in cilia and congenital disease.

  2020cites this paper
• Paired Box 9 (PAX9), the RNA polymerase II transcription factor, regulates human ribosome biogenesis and craniofacial development

  2020cites this paper
• DLG5 variants are associated with multiple congenital anomalies including ciliopathy phenotypes

  2020cites this paper
• Defective heart chamber growth and myofibrillogenesis after knockout of adprhl1 gene function by targeted disruption of the ancestral catalytic active site

  2020cites this paper
• Familial Dilated Cardiomyopathy Associated With a Novel Combination of Compound Heterozygous TNNC1 Variants

  2020cites this paper
• Disrupted ER membrane protein complex-mediated topogenesis drives congenital neural crest defects.

  2020cites this paper
• Development of the Hypothalamus in Xenopus laevis

  2020cites this paper
• Retroelement Insertion in a CRISPR/Cas9 Editing Site in the Early Embryo Intensifies Genetic Mosaicism

  2019cites this paper
• Xenbase: Facilitating the Use of Xenopus to Model Human Disease

  2019cites this paper
• Modeling congenital kidney diseases in Xenopus laevis

  2019cites this paper
• A chromosome-scale genome assembly and dense genetic map for Xenopus tropicalis.

  2019cites this paper
• Visualizing flow in an intact CSF network using optical coherence tomography: implications for human congenital hydrocephalus

  2019cites this paper
• The Many Faces of Xenopus: Xenopus laevis as a Model System to Study Wolf–Hirschhorn Syndrome

  2019cites this paper
• Histone H2B monoubiquitination regulates heart development via epigenetic control of cilia motility

  2019cites this paper
• Advancing genetic and genomic technologies deepen the pool for discovery in Xenopus tropicalis

  2019cites this paper
• Regulation of endogenous phytohormones alters the fluoranthene content in Arabidopsis thaliana.

  2019cites this paper
• Xenopus: Driving the Discovery of Novel Genes in Patient Disease and Their Underlying Pathological Mechanisms Relevant for Organogenesis

  2019cites this paper
• Novel truncating mutations in CTNND1 cause a dominant craniofacial and cardiac syndrome

  2019cites this paper
• A role for Shh and Bmp4 in regulating the dorsal-ventral patterning of the developing pharyngeal region

  2019cites this paper
• An investigation of the role of Ttyh1 in neuronal development via CRISPR/Cas9 genetic knockouts in Xenopus Laevis

  2019cites this paper
• Navigating Xenbase: An Integrated Xenopus Genomics and Gene Expression Database.

  2018cites this paper
• The Role of Hand1 in the Development of the Lateral Plate Mesoderm in Xenopus

  2018cites this paper
• Desmoplakin is required for epidermal integrity and morphogenesis in the Xenopus laevis embryo

  2018cites this paper
• Functional Assays to Screen and Dissect Genomic Hits: Doubling Down on the National Investment in Genomic Research.

  2018cites this paper
• USING THE FROG EPIDERMIS TO UNCOVER DESMOSOME FUNCTION AND REGULATION IN THE DEVELOPING EMBRYO

  2018cites this paper
• WDR5 Stabilizes Actin Architecture to Promote Multiciliated Cell Formation.

  2018cites this paper
• Mechanical Strain Determines Cilia Length, Motility, and Planar Position in the Left-Right Organizer.

  2018influential citation
• Primordial Germ Cell Transplantation for CRISPR/Cas9-based Leapfrogging in Xenopus.

  2018cites this paper
• CRISPR/Cas9-mediated mutation in Xenopus laevis to study the cell biology of hereditary forms of blindness

  2018cites this paper
• Advancing Understanding of Amphibian Evolution, Ecology, Behavior, and Conservation with Massively Parallel Sequencing

  2018cites this paper
• Dynamin Binding Protein Is Required for Xenopus laevis Kidney Development

  2018influential citation
• Candidate Heterotaxy Gene FGFR4 Is Essential for Patterning of the Left-Right Organizer in Xenopus

  2018influential citation
• CRISPR/Cas9-Mediated Knockout of Rb1 in Xenopus tropicalis.

  2018cites this paper
• De novo pathogenic variants in neuronal differentiation factor 2 (NEUROD2) cause a form of early infantile epileptic encephalopathy

  2018cites this paper
• CRISPR/Cas9 disease models in zebrafish and Xenopus: The genetic renaissance of fish and frogs.

  2018cites this paper
• Cancer Models in Xenopus tropicalis by CRISPR/Cas9 Mediated Knockout of Tumor Suppressors.

  2018cites this paper
• CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalis.

  2018cites this paper
• Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome Engineering.

  2018cites this paper
• Genotyping of CRISPR/Cas9 Genome Edited Xenopus tropicalis.

  2018cites this paper
• A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair.

  2018cites this paper
• Genome Editing and Engineering

  2018cites this paper
• Extreme nuclear branching in healthy epidermal cells of the Xenopus tail fin

  2018influential citation