Heritable genome editing in C. elegans via a CRISPR-Cas9 system

We report the use of clustered, regularly interspaced, short palindromic repeats (CRISPR)-associated endonuclease Cas9 to target genomic sequences in the Caenorhabditis elegans germ line using single-guide RNAs that are expressed from a U6 small nuclear RNA promoter. Our results demonstrate that targeted, heritable genetic alterations can be achieved in C. elegans, providing a convenient and effective approach for generating loss-of-function mutants.

• Publication year

  2013

• Venue

  Nature Methods

• Publication date

  2013-06-30

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/nmeth.2532PMID 23817069PMCID 3822328
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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