Gene Expression Analysis of Microtubers of Potato Solanum tuberosum L. Induced in Cytokinin Containing Medium and Osmotic Stress

Potato microtuber productions through in vitro techniques are ideal propagules for producing high quality seed potatoes. Microtuber development is influenced by several factors, i.e., high content sucrose and cytokinins are among them. To understand a molecular mechanism of microtuberization using osmotic stress and cytokinin signaling will help us to elucidate this process. We demonstrate in this work a rapid and efficient protocol for microtuber development and gene expression analysis. Medium with high content of sucrose and gelrite supplemented with 2iP as cytokinin under darkness condition produced the higher quantity and quality of microtubers. Gene expression analysis of genes involved in the two-component signaling system (StHK1), cytokinin signaling, (StHK3, StHP4, StRR1) homeodomains (WUSCHEL, POTH1, BEL5), auxin signaling, ARF5, carbon metabolism (TPI, TIM), protein synthesis, NAC5 and a morphogenetic regulator of tuberization (POTH15) was performed by qPCR real time. Differential gene expression was observed during microtuber development. Gene regulation of two component and cytokinin signaling is taking place during this developmental process, yielding more microtubers. Further analysis of each component is required to elucidate it.

• Publication year

  2021

• Venue

  Plants

• Publication date

  2021-04-27

• Fields of study

  Biology, Agricultural and Food Sciences, Medicine, Environmental Science

• Identifiers
  DOI 10.3390/plants10050876PMID 33925316PMCID 8146008
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Rapid analysis of GBSS1 and Vinv genes expressed in potato tubers using microtubers produced in liquid culture medium

  2020cited by this paper
• An integrative overview of physiological and proteomic changes of cytokinin-induced potato (Solanum tuberosum L.) tuber development in vitro.

  2020cited by this paper
• The Role of Mechanoperception in Plant Cell Wall Integrity Maintenance

  2020cited by this paper
• Cytokinin action in response to abiotic and biotic stresses in plants.

  2019cited by this paper
• Modeling of Protein–Protein Interactions in Cytokinin Signal Transduction

  2019cited by this paper
• STRING v11: protein–protein association networks with increased coverage, supporting functional discovery in genome-wide experimental datasets

  2018cited by this paper
• Comparative Proteomic Analysis of Potato (Solanum tuberosum L.) Tuberization In Vitro Regulated by IAA

  2018cited by this paper
• Cytokinin perception in potato: new features of canonical players

  2018influential reference
• Cytokinin Signaling in Plant Response to Abiotic Stresses

  2017cited by this paper
• Type-B ARABIDOPSIS RESPONSE REGULATORs Specify the Shoot Stem Cell Niche by Dual Regulation of WUSCHEL[OPEN]

  2017cited by this paper
• Changes in cytokinins are sufficient to alter developmental patterns of defense metabolites in Nicotiana attenuata

  2017cited by this paper
• Auxin synthesis gene tms1 driven by tuber-specific promoter alters hormonal status of transgenic potato plants and their responses to exogenous phytohormones

  2017cited by this paper
• Key players associated with tuberization in potato: potential candidates for genetic engineering

  2017cited by this paper
• The Multiple Signals That Control Tuber Formation1[OPEN]

  2017cited by this paper
• From Stress to Embryos: Some of the Problems for Induction and Maturation of Somatic Embryos.

  2016cited by this paper
• Into the Root: How Cytokinin Controls Rhizobial Infection.

  2016cited by this paper
• CYTOKININ RESPONSE FACTOR2 (CRF2) and CRF3 Regulate Lateral Root Development in Response to Cold Stress in Arabidopsis

  2016cited by this paper
• Arabidopsis type B cytokinin response regulators ARR1, ARR10, and ARR12 negatively regulate plant responses to drought

  2016cited by this paper
• Interactions between cytokinin signalling and abiotic stress responses.

  2015cited by this paper
• Expression of auxin synthesis gene tms1 under control of tuber-specific promoter enhances potato tuberization in vitro.

  2015cited by this paper
• Establishment of embryonic shoot–root axis is involved in auxin and cytokinin response during Arabidopsis somatic embryogenesis

  2015cited by this paper
• Roles of osmoprotectants in improving salinity and drought tolerance in plants: a review

  2015cited by this paper
• STM sustains stem cell function in the Arabidopsis shoot apical meristem and controls KNOX gene expression independently of the transcriptional repressor AS1

  2014cited by this paper
• UniProt: a hub for protein information

  2014cited by this paper
• The nascent polypeptide‐associated complex is a key regulator of proteostasis

  2013cited by this paper
• Auxin: simply complicated.

  2013cited by this paper
• A cytokinin-activating enzyme promotes tuber formation in tomato.

  2013cited by this paper
• The effects of auxin and strigolactones on tuber initiation and stolon architecture in potato

  2012cited by this paper
• Characterization of stress and methylglyoxal inducible triose phosphate isomerase (OscTPI) from rice

  2012cited by this paper
• Crops that feed the world 8: Potato: are the trends of increased global production sustainable?

  2012cited by this paper
• A rice orthologue of the ABA receptor, OsPYL/RCAR5, is a positive regulator of the ABA signal transduction pathway in seed germination and early seedling growth.

  2012cited by this paper
• Arabidopsis Response Regulator1 and Arabidopsis Histidine Phosphotransfer Protein2 (AHP2), AHP3, and AHP5 Function in Cold Signaling1[W][OA]

  2012cited by this paper
• Role of the Putative Osmosensor Arabidopsis Histidine Kinase1 in Dehydration Avoidance and Low-Water-Potential Response1[W][OA]

  2012cited by this paper
• Analysis of Cytokinin Mutants and Regulation of Cytokinin Metabolic Genes Reveals Important Regulatory Roles of Cytokinins in Drought, Salt and Abscisic Acid Responses, and Abscisic Acid Biosynthesis[C][W]

  2011cited by this paper
• Common and unique elements of the ABA-regulated transcriptome of Arabidopsis guard cells

  2011cited by this paper
• The Sol Genomics Network (solgenomics.net): growing tomatoes using Perl

  2010cited by this paper
• Potential utilization of NAC transcription factors to enhance abiotic stress tolerance in plants by biotechnological approach

  2010cited by this paper
• MIQE précis: Practical implementation of minimum standard guidelines for fluorescence-based quantitative real-time PCR experiments

  2010cited by this paper
• The polar auxin transport inhibitor NPA impairs embryo morphology and increases the expression of an auxin efflux facilitator protein PIN during Picea abies somatic embryo development.

  2009cited by this paper
• Effect of exogenous ABA on somatic embryo maturation and germination in Persian walnut (Juglans regia L.)

  2008cited by this paper
• Analysis of the Arabidopsis Histidine Kinase ATHK1 Reveals a Connection between Vegetative Osmotic Stress Sensing and Seed Maturation[W][OA]

  2008cited by this paper
• Gibberellin metabolism and its regulation.

  2008cited by this paper
• Cytokinin: secret agent of symbiosis.

  2008cited by this paper
• Signalling the Induction of Tuber Formation

  2007cited by this paper
• Functional analysis of AHK1/ATHK1 and cytokinin receptor histidine kinases in response to abscisic acid, drought, and salt stress in Arabidopsis

  2007influential reference
• A BELL1-Like Gene of Potato Is Light Activated and Wound Inducible1[C][OA]

  2007cited by this paper
• INTEGRATED NUTRIENT MANAGEMENT FOR THE PRODUCTIVITY AND QUALITY IMPROVEMENT OF POTATO UNDER IRRIGATED CONDITION

  2007cited by this paper
• Advances in Cytokinin Signaling

  2007cited by this paper
• Role of cytokinin and auxin in shaping root architecture: regulating vascular differentiation, lateral root initiation, root apical dominance and root gravitropism.

  2006cited by this paper
• Proteomic analysis of salt stress‐responsive proteins in rice root

  2005cited by this paper
• Cytokinin signaling.

  2005cited by this paper
• Involvement of endogenous gibberellins in potato tuber dormancy and early sprout growth: a critical assessment.

  2004cited by this paper
• Arabidopsis response regulator, ARR22, ectopic expression of which results in phenotypes similar to the wol cytokinin-receptor mutant.

  2004cited by this paper
• Studies on the role of the Arabidopsis gene MONOPTEROS in vascular development and plant cell axialization

  2004cited by this paper
• Plant response regulators implicated in signal transduction and circadian rhythm.

  2004cited by this paper
• Perception and signal transduction of cytokinins.

  2003cited by this paper
• Tuber storage proteins.

  2003cited by this paper
• Cytokinin signal perception and transduction.

  2003cited by this paper
• Overexpression of a Knotted-Like Homeobox Gene of Potato Alters Vegetative Development by Decreasing Gibberellin Accumulation1

  2003cited by this paper
• Potato microtuber production and performance: A review

  2003influential reference
• Interacting Transcription Factors from the Three-Amino Acid Loop Extension Superclass Regulate Tuber Formation1

  2003influential reference
• The WUSCHEL and SHOOTMERISTEMLESS genes fulfil complementary roles in Arabidopsis shoot meristem regulation.

  2002cited by this paper
• Proteomic analysis of rice leaves during drought stress and recovery

  2002cited by this paper
• Two-Component Signal Transduction Pathways in Arabidopsis1

  2002cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• dbSNP: the NCBI database of genetic variation

  2001cited by this paper
• Molecular and biochemical triggers of potato tuber development.

  2001cited by this paper
• The Arabidopsis AHK4 histidine kinase is a cytokinin-binding receptor that transduces cytokinin signals across the membrane.

  2001cited by this paper
• The Arabidopsis BELL1 and KNOX TALE Homeodomain Proteins Interact through a Domain Conserved between Plants and Animals Article, publication date, and citation information can be found at www.aspb.org/cgi/doi/10.1105/tpc.010161.

  2001influential reference
• Functional Analysis of the Conserved Domains of a Rice KNOX Homeodomain Protein, OSH15

  2001cited by this paper
• Sucrose utilization during potato microtuber growth in bioreactors

  2000cited by this paper
• The Saccharomyces cerevisiae Sln1p-Ssk1p two-component system mediates response to oxidative stress and in an oxidant-specific fashion.

  2000cited by this paper
• Influence of gelling agents on culture medium gel strength, water availability, tissue water potential, and maturation response in embryogenic cultures of Pinus strobus L.

  2000cited by this paper
• Ethylene: a gaseous signal molecule in plants.

  2000cited by this paper
• A possible role for pyrophosphate in the coordination of cytosolic and plastidial carbon metabolism within the potato tuber.

  2000cited by this paper
• A Transmembrane Hybrid-Type Histidine Kinase in Arabidopsis Functions as an Osmosensor

  1999cited by this paper
• Multiple signaling pathways control tuber induction in potato

  1999cited by this paper
• Protein changes in response to progressive water deficit in maize . Quantitative variation and polypeptide identification

  1998cited by this paper
• The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro

  1998cited by this paper
• Light regulation of anthocyanin, flavonoid and phenolic acid biosynthesis in potato minitubers in vitro

  1998cited by this paper
• Metabolic implications of stress-induced proline accumulation in plants

  1997cited by this paper
• Possible involvement of jasmonates in various morphogenic events

  1997cited by this paper
• The SHOOT MERISTEMLESS gene is required for maintenance of undifferentiated cells in Arabidopsis shoot and floral meristems and acts at a different regulatory level than the meristem genes WUSCHEL and ZWILLE.

  1996cited by this paper
• Stability of fingerprints of Solanum tuberosum plants derived from conventional tubers and vitrotubers

  1996cited by this paper
• INVESTIGATIONS INTO THE ROLE OF SUCROSE IN POTATO CV. ESTIMA MICROTUBER PRODUCTION IN VITRO

  1995cited by this paper
• Gelrite and Agar Differently Influence Cytokinin-Sensitivity of a Moss

  1995cited by this paper
• Gene expression and carbohydrate content during stolon to tuber transition in potatoes (Solanum tuberosum)

  1994cited by this paper
• The origin of the bifunctional dihydrofolate reductase-thymidylate synthase isogenes of Arabidopsis thaliana.

  1993cited by this paper
• The role of the monopteros gene in organising the basal body region of the Arabidopsis embryo

  1993cited by this paper
• Potato tuber-inducing activities of jasmonic acid and related compounds

  1991cited by this paper
• Jasmonates and their role in plant growth and development, with special reference to the control of potato tuberization: A review

  1991cited by this paper
• Jasmonic Acid induces tuberization of potato stolons cultured in vitro.

  1991cited by this paper
• In vitro tuberization in transgenic potatoes harboring ß-glucoronidase linked to a patatin promoter: effects of sucrose levels and photoperiods

  1991cited by this paper
• Patatin and four serine proteinase inhibitor genes are differentially expressed during potato tuber development

  1991cited by this paper
• Cytokinins in developing tuberous roots of sweet potato.

  1989cited by this paper
• Multielement analysis of agar and Gelrite impurities investigated by inductively coupled plasma emission spectrometry as well as physical properties of tissue culture media prepared with agar or the gellan gum Gelrite

  1988cited by this paper
• Tuberization and Hormones

  1984cited by this paper
• Effect of ABA and GA3 on tuberization and some chemical constituents of potato.

  1980cited by this paper
• Tuberization in Potato at High Temperatures: Responses to Gibberellin and Growth inhibitors

  1980cited by this paper
• Endogenous Cytokinins and Tuberization in the Potato (Solanum tuberosum L.)

  1976cited by this paper

• Potato microtuberization: its regulation and applications

  2025cites this paper
• Effect of moderate heat stress on tuberization of potato genotypes: Morphological and physiological changes using in vitro and ex vitro propagation

  2024cites this paper
• Plant Growth Adaptations of Chilean Native Potato Under Higher Soil and Air Temperature: A Field Comparative Study

  2024cites this paper
• Regulation of Different Lights on Energy Acquisitions, Microtuber Formation, and Growth of In Vitro-Grown Solanum tuberosum L.

  2024cites this paper
• Physiological, hormonal and molecular dynamics of root system architectural response to drought stress signaling in crops

  2024cites this paper
• The Impact of the Growth Regulators and Cultivation Conditions of Temporary Immersion Systems (TISs) on the Morphological Characteristics of Potato Explants and Microtubers

  2024cites this paper
• Analysis of Stress Response Genes in Microtuberization of Potato Solanum tuberosum L.: Contributions to Osmotic and Combined Abiotic Stress Tolerance

  2024cites this paper
• Exploring the Potential Role of Ribosomal Proteins to Enhance Potato Resilience in the Face of Changing Climatic Conditions

  2023cites this paper
• Transcriptome Analysis Reveals Molecular Signatures Associated with Apical Rooted Cutting (ARC) Technology in Seed Potato Production

  2023cites this paper
• Genome-wide identification, characterization, and expression analysis of the sweet potato (Ipomoea batatas [L.] Lam.) ARF, Aux/IAA, GH3, and SAUR gene families

  2023cites this paper
• Far-red spectrum leads to enhanced in vitro microtuberzation in potato (Solanum tuberosum cv. Sante)

  2023cites this paper
• Solanum tuberosum Microtuber Development under Darkness Unveiled through RNAseq Transcriptomic Analysis

  2022cites this paper
• In Vitro Propagation and Phytochemistry of Thymol-Producing Plants from a Horticultural Form of Thymus × josephi-angeli Mansanet & Aguil. (Lamiaceae)

  2022cites this paper
• Influence of Media Composition and Genotype on Potato (Solanum tuberosum L.) Microtuberization

  2021cites this paper