Paxillin.

Paxillin is a 68 kDa cytoplasmic protein that localizes to discrete sites of cell attachment to the extracellular matrix called focal adhesions. It is a multi-domain adapter protein capable of interacting with several structural and signaling proteins including vinculin, FAK, PYK2, Src and Crk. Phosphorylation of paxillin in response to integrin-mediated cell adhesion and growth factor stimulation regulates some of these interactions. Thus, paxillin functions as a scaffold for the recruitment of molecules into a signal transduction complex that is closely apposed to the plasma membrane. This is likely to facilitate the efficient processing of external stimuli that modulate important cellular events including cell adhesion, cell motility and growth control. Since paxillin interacts with several proteins known to cause cell transformation, the binding sites for these proteins on paxillin represent potential targets for therapeutic agents.

• Publication year

  2020

• Venue

  International Journal of Biochemistry and Cell Biology

• Publication date

  2020-02-02

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.32388/xzgv42PMID 9785458
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Serine and threonine phosphorylation of the paxillin LIM domains regulates paxillin focal adhesion localization and cell adhesion to fibronectin.

  1998cited by this paper
• Association of Bovine Papillomavirus Type 1 E6 oncoprotein with the focal adhesion protein paxillin through a conserved protein interaction motif

  1998cited by this paper
• Cell Adhesion Kinase β Forms a Complex with a New Member, Hic-5, of Proteins Localized at Focal Adhesions*

  1998cited by this paper
• Mitosis specific serine phosphorylation and downregulation of one of the focal adhesion protein, paxillin

  1997cited by this paper
• Adhesion of fibroblasts to fibronectin stimulates both serine and tyrosine phosphorylation of paxillin.

  1997cited by this paper
• Assembly of focal adhesions: progress, paradigms, and portents.

  1996cited by this paper
• Identification of LIM3 as the principal determinant of paxillin focal adhesion localization and characterization of a novel motif on paxillin directing vinculin and focal adhesion kinase binding

  1996cited by this paper
• pp125FAK-dependent tyrosine phosphorylation of paxillin creates a high-affinity binding site for Crk

  1995cited by this paper
• Direct association of pp125FAK with paxillin, the focal adhesion- targeting mechanism of pp125FAK

  1995cited by this paper
• Integrins: emerging paradigms of signal transduction.

  1995cited by this paper
• Integrins and signal transduction pathways: the road taken.

  1995cited by this paper
• Convergent signalling in the action of integrins, neuropeptides, growth factors and oncogenes.

  1995cited by this paper
• Molecular Cloning Of Human Paxillin, a Focal Adhesion Protein Phosphorylated by P210BCR/ABL(*)

  1995cited by this paper
• Characterization of Tyrosine Phosphorylation of Paxillin in Vitro by Focal Adhesion Kinase (*)

  1995cited by this paper
• Primary sequence of paxillin contains putative SH2 and SH3 domain binding motifs and multiple LIM domains: identification of a vinculin and pp125Fak-binding region.

  1994cited by this paper
• Paxillin: A cytoskeletal target for tyrosine kinases

  1994cited by this paper
• Detection of Src homology 3-binding proteins, including paxillin, in normal and v-Src-transformed Balb/c 3T3 cells.

  1993cited by this paper
• Paxillin: a new vinculin-binding protein present in focal adhesions

  1990cited by this paper

• Integrin αvβ3 as a Non-Genomic Estrogen Receptor in Breast Cancer for Signaling Pathways and Crosstalk

  2025cites this paper
• Platelet rich plasma promotes cell migration of adipose-derived stem cells by up-regulation of focal adhesion kinase and paxillin

  2025cites this paper
• α-Catenin and Piezo1 Mediate Cell Mechanical Communication via Cell Adhesions

  2024cites this paper
• differentiation factor-9 promotes adhesive and motile capacity of prostate cancer cells by up-regulating FAK and Paxillin via Smad dependent pathway

  2023cites this paper
• Paxillin regulates Rab5-mediated vesicle motility through modulating microtubule acetylation

  2023cites this paper
• A Systems Biology Approach for Addressing Cisplatin Resistance in Non-Small Cell Lung Cancer

  2023cites this paper
• The Paxillin MoPax1 Activates Mitogen-Activated Protein (MAP) Kinase Signaling Pathways and Autophagy through MAP Kinase Activator MoMka1 during Appressorium-Mediated Plant Infection by the Rice Blast Fungus Magnaporthe oryzae

  2022cites this paper
• Two macrophages, osteoclasts and microglia: from development to pleiotropy

  2021cites this paper
• RPS6 phosphorylation occurs to a greater extent in the periphery of human skeletal muscle fibers, near focal adhesions, after anabolic stimuli.

  2021cites this paper
• Endothelial Focal Adhesions Are Functional Obstacles for Leukocytes During Basolateral Crawling

  2021cites this paper
• Paxillin mediates ATP-induced activation of P2X7 receptor and NLRP3 inflammasome

  2020cites this paper
• Characterization of the inhibition mechanism of a tissuefactor inhibiting single-chain variable fragment: a combined computational approach

  2020cites this paper
• Comparative neurotranscriptomics reveal widespread species differences associated with bonding

  2020cites this paper
• Identification of hub genes involved in apparent metabolizable energy of chickens

  2020cites this paper
• In Full Force. Mechanotransduction and Morphogenesis during Homeostasis and Tissue Regeneration

  2020cites this paper
• A Non-genetic Mechanism Involving the Integrin β4/Paxillin Axis Contributes to Chemoresistance in Lung Cancer

  2020cites this paper
• RAB6 and microtubules restrict protein secretion to focal adhesions

  2019cites this paper
• RAB6 and microtubules restrict secretion to focal adhesions

  2018cites this paper
• Increased Paxillin expression in prostate cancer is associated with advanced pathological features, lymph node metastases and biochemical recurrence

  2018cites this paper
• The effect of midkine on growth factors and oxidative status in an experimental wound model in diabetic and healthy rats.

  2017cites this paper
• Wavelet Imaging on Multiple Scales (WIMS) reveals focal adhesion distributions, dynamics and coupling between actomyosin bundle stability

  2017cites this paper
• Investigating the role of protein tyrosine phosphatase alpha in focal adhesion dynamics and cell migration

  2017cites this paper
• In-situ coupling between kinase activities and protein dynamics within single focal adhesions

  2016cites this paper
• Scleral fibroblast response to experimental glaucoma in mice

  2016cites this paper
• The role of focal adhesion kinases and adaptor proteins in integrin-dependent signaling

  2016cites this paper
• Neuronal Wiskott‐Aldrich syndrome protein regulates TGF‐β1‐mediated lung vascular permeability

  2016cites this paper
• Biological characterization of three immortalized esophageal epithelial cell lines.

  2016cites this paper
• Exchange protein activated by cAMP (EPAC) controls migration of vascular smooth muscle cells in concentration and time-dependent manner

  2015cites this paper
• Subcellular stretch-induced cytoskeletal response of single fibroblasts within 3D designer scaffolds.

  2015cites this paper
• Kindlin‐2 phosphorylation by Src at Y193 enhances Src activity and is involved in Migfilin recruitment to the focal adhesions

  2015cites this paper
• MicroRNA-212 functions as an epigenetic-silenced tumor suppressor involving in tumor metastasis and invasion of gastric cancer through down-regulating PXN expression.

  2015cites this paper
• High calcium concentration in bones promotes bone metastasis in renal cell carcinomas expressing calcium-sensing receptor

  2014cites this paper
• Paxillin regulates vascular endothelial growth factor A-induced in vitro angiogenesis of human umbilical vein endothelial cells

  2014cites this paper
• Cellular Adhesion Gene SELP Is Associated with Rheumatoid Arthritis and Displays Differential Allelic Expression

  2014cites this paper
• Prognostic value of EZH2, paxillin expression and DNA ploidy of breast adenocarcinoma: correlation to pathologic predictors.

  2013cites this paper
• Retinoic acid induces adhesion and migration in NB4 cells through Pyk2 signaling.

  2013cites this paper
• A Role for the Protein Tyrosine Phosphatase CD45 in Macrophage Adhesion through the Regulation of Paxillin Degradation

  2013cites this paper
• Vinculin, cell mechanics and tumour cell invasion

  2013cites this paper
• VANGL2 regulates membrane trafficking of MMP14 to control cell polarity and migration

  2012cites this paper
• Paxillin regulates pulmonary arterial smooth muscle cell function in pulmonary hypertension.

  2012cites this paper
• LNK (SH2B3) is a key regulator of integrin signaling in endothelial cells and targets α‐parvin to control cell adhesion and migration

  2012cites this paper
• Mechanotransduction and focal adhesions

  2012cites this paper
• MicroRNAs Profiling in Murine Models of Acute and Chronic Asthma: A Relationship with mRNAs Targets

  2011cites this paper
• Effects of Simvastatin and Fluvastatin on Endothelial Invasion

  2011cites this paper
• Connecting chronic and recurrent stress to vascular dysfunction: no relaxed role for the renin-angiotensin system.

  2011cites this paper
• Regulation of focal adhesions by flightless i involves inhibition of paxillin phosphorylation via a Rac1-dependent pathway.

  2011cites this paper
• Effect of Flightless protein on skin architecture, cellular responses and Epidermolysis Bullosa.

  2011cites this paper
• Growth and differentiation factor-9 promotes adhesive and motile capacity of prostate cancer cells by up-regulating FAK and Paxillin via Smad dependent pathway.

  2010cites this paper
• Adhesion of ST6Gal I-mediated human colon cancer cells to fibronectin contributes to cell survival by integrin beta1-mediated paxillin and AKT activation.

  2010cites this paper
• Cleavage of galectin‐3 by matrix metalloproteases induces angiogenesis in breast cancer

  2010cites this paper
• Increased Rac2 mRNA expression in peripheral blood during human corneal graft rejection

  2009cites this paper
• Vascular smooth muscle cells promote endothelial cell adhesion via microtubule dynamics and activation of paxillin and the extracellular signal-regulated kinase (ERK) pathway in a co-culture system.

  2009cites this paper
• Les rôles distincts des isoformes de myosine II non-musculaire dans des processus cellulaires impliquant le cytosquelette d'actine

  2009cites this paper
• Paxillin is involved in the differential regulation of endothelial barrier by HGF and VEGF.

  2009cites this paper
• Enhanced interaction between focal adhesion and adherens junction proteins: involvement in sphingosine 1-phosphate-induced endothelial barrier enhancement.

  2009cites this paper
• The Role of Vinculin in the Regulation of the Mechanical Properties of Cells

  2009cites this paper
• Expression and Organization of Basement Membranes and Focal Adhesion Proteins in Pregnant Myometrium is Regulated by Uterine Stretch

  2009cites this paper
• Isothiocyanate-functionalized RGD peptides for tailoring cell-adhesive surface patterns.

  2008cites this paper
• JNK regulates cell migration through promotion of tyrosine phosphorylation of paxillin.

  2008cites this paper
• Activation of c-Src and Fyn Kinases by Protein-tyrosine Phosphatase RPTPα Is Substrate-specific and Compatible with Lipid Raft Localization*

  2008cites this paper
• Integrin‐dependent translational control: Implication in cancer progression

  2008cites this paper
• Pressure activates colon cancer cell adhesion via paxillin phosphorylation, Crk, Cas, and Rac1

  2008cites this paper
• Paxillin and ponsin interact in nascent costameres of muscle cells.

  2007cites this paper
• CNTO 95, a fully human anti αv integrin antibody, inhibits cell signaling, migration, invasion, and spontaneous metastasis of human breast cancer cells

  2007cites this paper
• Comparison of the effects of differentially sulphated bovine kidney- and porcine intestine-derived heparan sulphate on breast carcinoma cellular behaviour.

  2007cites this paper
• Influence of Systematically Varied Nano-Scale Topography on Cell Morphology and Adhesion

  2007cites this paper
• Focal adhesion kinase and p53 signaling in cancer cells.

  2007cites this paper
• Laminins in Peripheral Nerve Development and Muscular Dystrophy

  2007cites this paper
• Differential expression of the FAK family kinases in rheumatoid arthritis and osteoarthritis synovial tissues

  2007cites this paper
• Homocysteine-induced biochemical stress predisposes to cytoskeletal remodeling in stretched endothelial cells

  2007cites this paper
• Proapoptotic function of the nuclear Crk II adaptor protein.

  2007cites this paper
• Interaction of Pyk2 and PTP-PEST with leupaxin in prostate cancer cells.

  2007cites this paper
• Green tea induces annexin-I expression in human lung adenocarcinoma A549 cells: involvement of annexin-I in actin remodeling

  2007cites this paper
• Halothane affects focal adhesion proteins in the A 549 cells

  2006cites this paper
• RAFTK/Pyk2 mediates LPA-induced PC12 cell migration.

  2006cites this paper
• Minimal features of paxillin that are required for the tyrosine phosphorylation of focal adhesion kinase.

  2006cites this paper
• Involvement of the paxillin pathway in JB6 Cl41 cell transformation.

  2006cites this paper
• PP2 inhibits glutamate release from nerve endings by affecting vesicle mobilization

  2005cites this paper
• Cellular functions of endoplasmic reticulum chaperones calreticulin, calnexin, and ERp57.

  2005cites this paper
• Differential effects of shear stress and cyclic stretch on focal adhesion remodeling, site-specific FAK phosphorylation, and small GTPases in human lung endothelial cells.

  2005cites this paper
• Conditional knockout of focal adhesion kinase in endothelial cells reveals its role in angiogenesis and vascular development in late embryogenesis

  2005cites this paper
• Angiotensin II induces focal adhesion kinase/paxillin phosphorylation and cell migration in human umbilical vein endothelial cells.

  2005cites this paper
• Tension development during contractile stimulation of smooth muscle requires recruitment of paxillin and vinculin to the membrane.

  2004cites this paper
• Human Mesenchymal Stem Cells Tissue Development in 3D PET Matrices

  2004cites this paper
• Regulation of integrin-mediated cellular responses through assembly of a CAS/Crk scaffold.

  2004cites this paper
• Signal transduction pathways activated in human pulmonary endothelial cells by OxPAPC, a bioactive component of oxidized lipoproteins.

  2004cites this paper
• Array-based analysis of gene expression in childhood acute lymphoblastic leukemia.

  2004cites this paper
• Focal adhesion regulation of cell behavior.

  2004cites this paper
• High-throughput investigation of osteoblast response to polymer crystallinity: influence of nanometer-scale roughness on proliferation.

  2004cites this paper
• Basis and importance of Src as a target in cancer.

  2004cites this paper
• Ligand stimulation of CD155alpha inhibits cell adhesion and enhances cell migration in fibroblasts.

  2004cites this paper
• Entamoeba histolytica: a beta 1 integrin-like fibronectin receptor assembles a signaling complex similar to those of mammalian cells.

  2003cites this paper
• Effect of increased expression of cytoskeletal protein vinculin on ischemia-reperfusion injury in ventricular myocytes.

  2003cites this paper
• Mechanisms of Hepatocyte Growth Factor–Mediated Vascular Smooth Muscle Cell Migration

  2003cites this paper
• Inhibition of alpha4-integrin stimulates epicardial-mesenchymal transformation and alters migration and cell fate of epicardially derived mesenchyme.

  2003cites this paper
• Differential effect of wounding on actin and its associated proteins, paxillin and gelsolin, in fetal skin explants.

  2003cites this paper
• S1P induces FA remodeling in human pulmonary endothelial cells: role of Rac, GIT1, FAK, and paxillin.

  2003cites this paper
• Leupaxin Is a Critical Adaptor Protein in the Adhesion Zone of the Osteoclast

  2003cites this paper
• Ras-induced serine phosphorylation of the focal adhesion protein paxillin is mediated by the Raf-->MEK-->ERK pathway.

  2003cites this paper
• Ionizing radiation modules of the expression and tyrosine phosphorylation of the focal adhesion‐associated proteins focal adhesion kinase (FAK) and its substrates p130cas and paxillin in A549 human lung carcinoma cells in vitro

  2003cites this paper