Non-human primate pluripotent stem cells for the preclinical testing of regenerative therapies

Non-human primates play a key role in the preclinical validation of pluripotent stem cell-based cell replacement therapies. Pluripotent stem cells used as advanced therapy medical products boost the possibility to regenerate tissues and organs affected by degenerative diseases. Therefore, the methods to derive human induced pluripotent stem cell and embryonic stem cell lines following clinical standards have quickly developed in the last 15 years. For the preclinical validation of cell replacement therapies in non-human primates, it is necessary to generate non-human primate pluripotent stem cell with a homologous quality to their human counterparts. However, pluripotent stem cell technologies have developed at a slower pace in non-human primates in comparison with human cell systems. In recent years, however, relevant progress has also been made with non-human primate pluripotent stem cells. This review provides a systematic overview of the progress and remaining challenges for the generation of non-human primate induced pluripotent stem cells/embryonic stem cells for the preclinical testing and validation of cell replacement therapies. We focus on the critical domains of (1) reprogramming and embryonic stem cell line derivation, (2) cell line maintenance and characterization and, (3) application of non-human primate pluripotent stem cells in the context of selected preclinical studies to treat cardiovascular and neurodegenerative disorders performed in non-human primates.

• Publication year

  2022

• Venue

  Neural Regeneration Research

• Publication date

  2022-02-08

• Fields of study

  Medicine

• Identifiers
  DOI 10.4103/1673-5374.335689PMID 35142660PMCID 8848615
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• FIU Digital Commons FIU Digital Commons Global Burden of Cardiovascular Diseases and Risk Factors, Global Burden of Cardiovascular Diseases and Risk Factors, 1990-2019: Update From the GBD 2019 Study 1990-2019: Update From the GBD 2019 Study

  2022cited by this paper
• Autologous transplant therapy alleviates motor and depressive behaviors in parkinsonian monkeys

  2021influential reference
• Establishment of novel common marmoset embryonic stem cell lines under various conditions.

  2021influential reference
• Generation of Marmoset Monkey iPSCs with Self-Replicating VEE-mRNAs in Feeder-Free Conditions.

  2021cited by this paper
• Generation and Cultivation of Transgene-Free Macaque and Baboon iPSCs Under Chemically Defined Conditions.

  2021influential reference
• Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species

  2021cited by this paper
• A piggyBac-based platform for genome editing and clonal rhesus macaque iPSC line derivation

  2021cited by this paper
• No Tumorigenicity of Allogeneic Induced Pluripotent Stem Cells in Major Histocompatibility Complex-matched Cynomolgus Macaques

  2021cited by this paper
• Non-Human Primate iPSC Generation, Cultivation, and Cardiac Differentiation under Chemically Defined Conditions

  2020cited by this paper
• Regenerative therapy for spinal cord injury using iPSC technology

  2020cited by this paper
• Robust detection of undifferentiated iPSC among differentiated cells

  2020cited by this paper
• Diminished expression of major histocompatibility complex facilitates the use of human induced pluripotent stem cells in monkey

  2020influential reference
• A non-invasive method to generate induced pluripotent stem cells from primate urine

  2020cited by this paper
• Pluripotent Stem Cell-Based Cell Therapy-Promise and Challenges.

  2020cited by this paper
• Development of genetic quality tests for good manufacturing practice-compliant induced pluripotent stem cells and their derivatives

  2020cited by this paper
• Maturation of Human Pluripotent Stem Cell-Derived Cerebellar Neurons in the Absence of Co-culture

  2020cited by this paper
• Cardiomyocyte Maturation: New Phase in Development.

  2020cited by this paper
• Global Burden of Cardiovascular Diseases and Risk Factors, 1990–2019

  2020cited by this paper
• Controlling the Switch from Neurogenesis to Pluripotency during Marmoset Monkey Somatic Cell Reprogramming with Self-Replicating mRNAs and Small Molecules

  2020influential reference
• Apoptosis, G1 Phase Stall, and Premature Differentiation Account for Low Chimeric Competence of Human and Rhesus Monkey Naive Pluripotent Stem Cells

  2020cited by this paper
• MHC matching fails to prevent long-term rejection of iPSC-derived neurons in non-human primates

  2019cited by this paper
• Highly efficient induction of primate iPS cells by combining RNA transfection and chemical compounds

  2019influential reference
• Developmental Gene Expression Differences between Humans and Mammalian Models

  2019cited by this paper
• Advances in Pluripotent Stem Cells: History, Mechanisms, Technologies, and Applications

  2019cited by this paper
• Stem cell therapy for Parkinson’s disease using non-human primate models

  2019influential reference
• Species-specific maturation profiles of human, chimpanzee and bonobo neural cells

  2019cited by this paper
• Baboon induced pluripotent stem cell generation by piggyBac transposition of reprogramming factors

  2019cited by this paper
• Cross-species blastocyst chimerism between nonhuman primates using iPSCs

  2019cited by this paper
• Establishment of induced pluripotent stem cells from common marmoset fibroblasts by RNA-based reprogramming.

  2019cited by this paper
• Optimization of culture conditions for the derivation and propagation of baboon (Papio anubis) induced pluripotent stem cells

  2018influential reference
• A Comparative Assessment of Human and Chimpanzee iPSC-derived Cardiomyocytes with Primary Heart Tissues

  2018cited by this paper
• Comparison of Non-human Primate versus Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes for Treatment of Myocardial Infarction

  2018cited by this paper
• Standards for Deriving Nonhuman Primate-Induced Pluripotent Stem Cells, Neural Stem Cells and Dopaminergic Lineage

  2018influential reference
• Human ESC-Derived Cardiomyocytes Restore Function in Infarcted Hearts of Non-Human Primates

  2018cited by this paper
• Induced Pluripotent Stem Cell-Derived Dopaminergic Neurons from Adult Common Marmoset Fibroblasts

  2017influential reference
• Mechanism of human somatic reprogramming to iPS cell

  2017cited by this paper
• An overview of nonhuman primates in aging research

  2017cited by this paper
• Lineage-Specific Differentiation Is Influenced by State of Human Pluripotency.

  2017cited by this paper
• Cellular Reprogramming Using Protein and Cell-Penetrating Peptides

  2017cited by this paper
• Defined Engineered Human Myocardium With Advanced Maturation for Applications in Heart Failure Modeling and Repair

  2017cited by this paper
• Autologous Induced Stem-Cell-Derived Retinal Cells for Macular Degeneration.

  2017cited by this paper
• The Immunogenicity and Immune Tolerance of Pluripotent Stem Cell Derivatives

  2017influential reference
• Nonhuman Primates and Translational Research-Cardiovascular Disease.

  2017influential reference
• Autologous Induced Stem‐Cell–Derived Retinal Cells for Macular Degeneration: Brief Report

  2017cited by this paper
• Generation of Macaca fascicularis iPS cell line ATCi-MF1 from adult skin fibroblasts using non-integrative Sendai viruses.

  2017cited by this paper
• The Role of Nonhuman Primate Animal Models in the Clinical Development of Pluripotent Stem Cell Therapies.

  2016influential reference
• Allogeneic transplantation of iPS cell-derived cardiomyocytes regenerates primate hearts

  2016influential reference
• The transcriptomes of novel marmoset monkey embryonic stem cell lines reflect distinct genomic features

  2016cited by this paper
• Induced pluripotent stem cell technology: a decade of progress

  2016cited by this paper
• Enhanced engraftment, proliferation, and therapeutic potential in heart using optimized human iPSC-derived cardiomyocytes

  2016cited by this paper
• 10th anniversary of iPS cells: the challenges that lie ahead.

  2016cited by this paper
• Marmoset induced pluripotent stem cells: Robust neural differentiation following pretreatment with dimethyl sulfoxide.

  2015cited by this paper
• cGMP-Manufactured Human Induced Pluripotent Stem Cells Are Available for Pre-clinical and Clinical Applications

  2015influential reference
• Non-Viral Generation of Marmoset Monkey iPS Cells by a Six-Factor-in-One-Vector Approach

  2015influential reference
• An improved ScoreCard to assess the differentiation potential of human pluripotent stem cells

  2015cited by this paper
• Autologous iPSC-derived dopamine neuron transplantation in a nonhuman primate Parkinson’s disease model

  2015cited by this paper
• Generation of Cynomolgus Monkey Chimeric Fetuses using Embryonic Stem Cells.

  2015cited by this paper
• Primate iPS cells as tools for evolutionary analyses.

  2014cited by this paper
• Nonhuman Primate Models in Translational Regenerative Medicine

  2014cited by this paper
• A comparison of non-integrating reprogramming methods

  2014influential reference
• A panel of induced pluripotent stem cells from chimpanzees: a resource for comparative functional genomics

  2014influential reference
• Generation of naive induced pluripotent stem cells from rhesus monkey fibroblasts.

  2014cited by this paper
• Improved Cell Therapy Protocols for Parkinson's Disease Based on Differentiation Efficiency and Safety of hESC‐, hiPSC‐, and Non‐Human Primate iPSC‐Derived Dopaminergic Neurons

  2013cited by this paper
• Cynomolgus monkey induced pluripotent stem cells established by using exogenous genes derived from the same monkey species.

  2013cited by this paper
• Ex Vivo Reconstitution of Arterial Endothelium by Embryonic Stem Cell-Derived Endothelial Progenitor Cells in Baboons

  2013cited by this paper
• Direct Comparison of Autologous and Allogeneic Transplantation of iPSC-Derived Neural Cells in the Brain of a Nonhuman Primate

  2013cited by this paper
• Derivation of Induced Pluripotent Stem Cells from the Baboon: A Nonhuman Primate Model for Preclinical Testing of Stem Cell Therapies

  2013cited by this paper
• Differential LINE-1 regulation in pluripotent stem cells of humans and other great apes

  2013cited by this paper
• Induced pluripotent stem cell-derived neural cells survive and mature in the nonhuman primate brain.

  2013cited by this paper
• Induction of Pluripotent Stem Cells from a Cynomolgus Monkey Using a Polycistronic Simian Immunodeficiency Virus–Based Vector, Differentiation Toward Functional Cardiomyocytes, and Generation of Stably Expressing Reporter Lines

  2012cited by this paper
• Efficient Derivation of Multipotent Neural Stem/Progenitor Cells from Non-Human Primate Embryonic Stem Cells

  2012cited by this paper
• Derivation of Neural Progenitors and Retinal Pigment Epithelium from Common Marmoset and Human Pluripotent Stem Cells

  2012cited by this paper
• Establishment of transgene-free induced pluripotent stem cells reprogrammed from human stem cells of apical papilla for neural differentiation

  2012cited by this paper
• Pre-Evaluated Safe Human iPSC-Derived Neural Stem Cells Promote Functional Recovery after Spinal Cord Injury in Common Marmoset without Tumorigenicity

  2012influential reference
• Induced Pluripotent Stem Cells Generated from Adult Bone Marrow–Derived Cells of the Nonhuman Primate (Callithrix jacchus) Using a Novel Quad-Cistronic and Excisable Lentiviral Vector

  2012cited by this paper
• Nonhuman Primate Induced Pluripotent Stem Cells in Regenerative Medicine

  2012cited by this paper
• Birds, primates, and spoken language origins: behavioral phenotypes and neurobiological substrates

  2012cited by this paper
• Reprogramming of somatic cells via TAT-mediated protein transduction of recombinant factors.

  2012cited by this paper
• Reference Maps of human ES and iPS cell variation enable high-throughput characterization of pluripotent cell lines.

  2011cited by this paper
• A more efficient method to generate integration-free human iPS cells

  2011cited by this paper
• Induced pluripotent stem cells from highly endangered species

  2011cited by this paper
• Induction of pluripotent stem cells from fetal and adult cynomolgus monkey fibroblasts using four human transcription factors

  2011cited by this paper
• Efficient Generation of Nonhuman Primate Induced Pluripotent Stem Cells

  2011cited by this paper
• A bioinformatic assay for pluripotency in human cells

  2011influential reference
• Transgene-free production of pluripotent stem cells using piggyBac transposons.

  2011cited by this paper
• Generation of induced pluripotent stem cells from newborn marmoset skin fibroblasts.

  2010influential reference
• Development of Defective and Persistent Sendai Virus Vector

  2010cited by this paper
• Reprogramming Huntington Monkey Skin Cells into Pluripotent Stem Cells

  2010cited by this paper
• Generating induced pluripotent stem cells from common marmoset (Callithrix jacchus) fetal liver cells using defined factors, including Lin28

  2010cited by this paper
• A call to standardize teratoma assays used to define human pluripotent cell lines.

  2010cited by this paper
• Efficient induction of transgene-free human pluripotent stem cells using a vector based on Sendai virus, an RNA virus that does not integrate into the host genome

  2009cited by this paper
• Generation of human induced pluripotent stem cells by direct delivery of reprogramming proteins.

  2009cited by this paper
• A novel embryonic stem cell line derived from the common marmoset monkey (Callithrix jacchus) exhibiting germ cell-like characteristics.

  2009cited by this paper
• Supplemental Data Generation of Human Induced Pluripotent Stem Cells by Direct Delivery of Reprogramming Proteins

  2009cited by this paper
• Establishment and characterization of baboon embryonic stem cell lines: an Old World Primate model for regeneration and transplantation research.

  2009cited by this paper
• Feeder-free derivation of induced pluripotent stem cells from adult human adipose stem cells

  2009cited by this paper
• Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts.

  2008influential reference
• Heterozygous Embryonic Stem Cell Lines Derived from Nonhuman Primate Parthenotes

  2008influential reference
• Pedigreed Primate Embryonic Stem Cells Express Homogeneous Familial Gene Profiles

  2007cited by this paper
• Producing primate embryonic stem cells by somatic cell nuclear transfer

  2007cited by this paper

• Non-human primate: the new frontier model of female reproductive engineering

  2025cites this paper
• Induced Pluripotent Stem Cell-Based Cancer Immunotherapy: Strategies and Perspectives

  2025cites this paper
• Addressing missing context in regulatory variation across primate evolution

  2025cites this paper
• Advancing Organoid Engineering for Tissue Regeneration and Biofunctional Reconstruction

  2024cites this paper
• Transgene-Free Cynomolgus Monkey iPSCs Generated under Chemically Defined Conditions

  2024cites this paper
• Cardiac differentiation of chimpanzee induced pluripotent stem cell lines with different subspecies backgrounds

  2024cites this paper
• Generation and characterization of inducible KRAB-dCas9 iPSCs from primates for cross-species CRISPRi

  2024cites this paper
• Non-human primate studies for cardiomyocyte transplantation—ready for translation?

  2024cites this paper
• Breaking the mold: 3D cell cultures reshaping the future of cancer research

  2024cites this paper
• An expedition in the jungle of pluripotent stem cells of non-human primates

  2023cites this paper
• Tissue Engineering and Stem Cell Therapy in Neurogenic Bladder Dysfunction: Current and Future Perspectives

  2023cites this paper
• Long non-coding RNA H19 regulates neurogenesis of induced neural stem cells in a mouse model of closed head injury

  2023cites this paper
• Embryoids, models, embryos? We need to take a new look at legal norms concerning the beginning of organismic development.

  2023cites this paper
• Non-human primate models of focal cortical ischemia for neuronal replacement therapy

  2023cites this paper
• Organoids and Their Research Progress in Plastic and Reconstructive Surgery

  2022cites this paper
• Stammzellforschung an und mit Affen: zwischen Grundlagen und Anwendung

  2022cites this paper