Molecular and morphologic changes during the epithelial-mesenchymal transformation of palatal shelf medial edge epithelium in vitro.

The fate of the medial edge epithelial (MEE) cells during palatal fusion has been proposed to be either programmed cell death or epithelial-mesenchymal transformation. Vital cell labeling techniques were used to mark the MEE and observe their fate during palatal fusion in vitro. Fetal mouse palatal shelves were labeled with Dil and allowed to proceed through fusion while maintained in an organ culture system. The tissues were examined at several stages of palatal fusion for the distribution of Dil, presence of specific antigens and ultrastructural appearance of the cells. The MEE labeled with Dil occupied a midline position at all stages of palatal fusion. Initially the cells had keratin intermediate filaments and were separated from the underlying mesenchyme by an intact basement membrane. During the process of fusion the basement membrane was degraded and the Dil-labeled MEE were in contact with the mesenchymal-derived extracellular matrix. In the late stages of fusion the Dil-labeled MEE altered their cellular morphology, had vimentin intermediate filaments, and were not associated with an identifiable basement membrane. Dil-labeled cells, without an epithelial phenotype, remained present in the midline of the completely fused palate. The data indicate that the MEE did not die but underwent a phenotypic transformation to viable mesenchymal cell types, which were retained in the palatal mesenchyme.

• Publication year

  1991

• Venue

  International Journal of Developmental Biology

• Publication date

  1991-12-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1387/IJDB.1801871PMID 1801871
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• The labeled medial edge epithelial cells did not die; they underwent epithelial-mesenchymal transformation into viable mesenchymal cell types that were retained in the palatal mesenchyme.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review
• As fusion progressed, the basement membrane was degraded, the labeled cells contacted mesenchymal-derived extracellular matrix, and late-stage labeled cells contained vimentin intermediate filaments.

  Confidence 0.95

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review
• At the onset of fusion, medial edge epithelial cells contained keratin intermediate filaments and were separated from the underlying mesenchyme by an intact basement membrane.

  Confidence 0.96

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review
• Dil-labeled medial edge epithelial cells remained in the midline throughout palatal fusion.

  Confidence 0.97

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review

• basement membrane

  extracellular structure

  The thin extracellular matrix layer that separates the epithelium from the underlying mesenchyme.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review
• dil labeling

  method, labeling technique

  A vital-cell labeling approach that uses the fluorescent dye Dil to mark cells in organ culture.

  Aliases: DiI labeling, Dil

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review
• epithelial-mesenchymal transformation

  cellular process, phenotypic transformation

  A phenotypic transition from epithelial characteristics toward mesenchymal-like morphology and marker expression.

  Aliases: EMT

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review
• keratin intermediate filaments

  cellular structure, marker

  Cytoskeletal filaments associated with epithelial cell phenotype in the palatal shelf epithelium.

  Aliases: keratin filaments

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review
• medial edge epithelial cells

  cell type

  The epithelial cells at the medial edge of fetal mouse palatal shelves that line the fusion seam.

  Aliases: MEE cells, MEE

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review
• mesenchymal-derived extracellular matrix

  extracellular matrix, tissue component

  Extracellular matrix material present at the fusion site and derived from mesenchymal tissue.

  Aliases: mesenchyme-derived extracellular matrix

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review
• palatal fusion

  developmental process

  The developmental process in which opposing palatal shelves join to form a continuous palate.

  Aliases: palate fusion

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review
• palatal mesenchyme

  tissue

  The connective tissue underlying the palatal epithelium and forming the interior tissue of the palatal shelf.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review
• vimentin intermediate filaments

  cellular structure, marker

  Cytoskeletal filaments associated with mesenchymal cell phenotype in the palatal fusion region.

  Aliases: vimentin filaments

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (n259mg7uxy) reviewq (76h6bfydm6) review

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