The history of DNA sequencing dates back to 1970s. During this period the two first generation nucleotide sequencing techniques were developed. Subsequently the Sanger's dideoxy method of sequencing gained popularity over Maxam and Gilbert's chemical method of sequencing. However, in the last decade, we have observed revolutionary changes in DNA sequencing technologies leading to the emergence of next-generation sequencing (NGS) techniques. NGS technologies have enhanced the throughput and speed of sequencing combined with bringing down the overall cost of the process over a time. The major applications of NGS technologies being genome sequencing and resequencing, transcriptomics, metagenomics in relation to plant-microbe interactions, exon and genome capturing, development of molecular markers and evolutionary studies. In this review, we present a broader picture of evolution of NGS tools, its various applications in crop plants, and future prospects of the technology for crop improvement.
Status and Prospects of Next Generation Sequencing Technologies in Crop Plants.
T. Sharma,B. N. Devanna,K. Kiran,P. Singh,K. Arora,P. Jain,I. Tiwari,Himanshu Dubey,B. Saklani,M. Kumari,Jyoti Singh,R. Jaswal,R. Kapoor,Deepak V. Pawar,Shruti Sinha,D. Bisht,A. Solanke,T. Mondal
Published 2018 in Current Issues in Molecular Biology
ABSTRACT
PUBLICATION RECORD
- Publication year
2018
- Venue
Current Issues in Molecular Biology
- Publication date
Unknown publication date
- Fields of study
Agricultural and Food Sciences, Medicine, Biology, Environmental Science
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
CITATION MAP
EXTRACTION MAP
CLAIMS
CONCEPTS
- crop improvement
The breeding and genetic enhancement of crop species to obtain desirable traits or better performance.
- crop plants
Agricultural plant species that serve as the biological focus for the sequencing applications discussed in the review.
- evolutionary studies
Comparative sequence analyses used to investigate evolutionary relationships and change.
- exon and genome capture
A targeted sequencing approach that enriches selected exonic or genomic regions before sequencing.
Aliases: capture sequencing
- genome sequencing and resequencing
Sequencing whole genomes and re-sequencing existing genomes to obtain or refine genomic information.
Aliases: genome sequencing, resequencing
- metagenomics in plant-microbe interactions
Sequencing-based analysis of mixed microbial and plant-associated communities in the context of their interactions.
Aliases: plant-microbe interactions metagenomics
- molecular markers
Heritable DNA variants used as markers for genetic analysis and breeding in crops.
- next-generation sequencing (ngs)
A newer generation of DNA sequencing technologies emphasized in the review for crop research applications.
Aliases: next generation sequencing, NGS
- sequencing cost
The overall expense associated with performing DNA sequencing, including trends in affordability over time.
Aliases: overall cost
- sequencing throughput and speed
The amount of sequencing output produced and the pace at which sequencing can be completed.
Aliases: throughput, speed
- transcriptomics
The study of RNA transcript populations expressed in crop plants using sequencing-based methods.
REFERENCES
CITED BY
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