Synergistic effect of WRI1 and DGAT1 coexpression on triacylglycerol biosynthesis in plants

Metabolic engineering approaches to increase plant oil levels can generally be divided into categories which increase fatty acid biosynthesis (‘Push’), are involved in TAG assembly (‘Pull’) or increase TAG storage/decrease breakdown (‘Accumulation’). In this study, we describe the surprising synergy when Push (WRI1) and Pull (DGAT1) approaches are combined. Co‐expression of these genes in the Nicotiana benthamiana transient leaf expression system resulted in TAG levels exceeding those expected from an additive effect and biochemical tracer studies confirmed increased flux of carbon through fatty acid and TAG synthesis pathways. Leaf fatty acid profile also synergistically shifts from polyunsaturated to monounsaturated fatty acids.

• Publication year

  2013

• Venue

  FEBS Letters

• Publication date

  2013-02-14

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.febslet.2012.12.018PMID 23313251
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Feedback regulation of plastidic acetyl-CoA carboxylase by 18:1-acyl carrier protein in Brassica napus

  2012influential reference
• Recruiting a New Substrate for Triacylglycerol Synthesis in Plants: The Monoacylglycerol Acyltransferase Pathway

  2012cited by this paper
• The Significance of Different Diacylgycerol Synthesis Pathways on Plant Oil Composition and Bioengineering

  2012cited by this paper
• Metabolic Engineering Plant Seeds with Fish Oil-Like Levels of DHA

  2012cited by this paper
• Does diacylglycerol serve as a signaling molecule in plants?

  2012cited by this paper
• Rapid Kinetic Labeling of Arabidopsis Cell Suspension Cultures: Implications for Models of Lipid Export from Plastids1[W][OA]

  2011cited by this paper
• Correction for Bourgis et al., Comparative transcriptome and metabolite analysis of oil palm and date palm mesocarp that differ dramatically in carbon partitioning

  2011cited by this paper
• Increasing the energy density of vegetative tissues by diverting carbon from starch to oil biosynthesis in transgenic Arabidopsis.

  2011cited by this paper
• Enhanced Seed Oil Production in Canola by Conditional Expression of Brassica napus LEAFY COTYLEDON1 and LEC1-LIKE in Developing Seeds1[W][OA]

  2011cited by this paper
• Comparative transcriptome and metabolite analysis of oil palm and date palm mesocarp that differ dramatically in carbon partitioning

  2011cited by this paper
• The pathway of triacylglycerol synthesis through phosphatidylcholine in Arabidopsis produces a bottleneck for the accumulation of unusual fatty acids in transgenic seeds.

  2011cited by this paper
• Tobacco as a production platform for biofuel: overexpression of Arabidopsis DGAT and LEC2 genes increases accumulation and shifts the composition of lipids in green biomass.

  2010cited by this paper
• Rapid expression of transgenes driven by seed-specific constructs in leaf tissue: DHA production

  2010cited by this paper
• Disruption of the Arabidopsis CGI-58 homologue produces Chanarin–Dorfman-like lipid droplet accumulation in plants

  2010cited by this paper
• Increasing seed mass and oil content in transgenic Arabidopsis by the overexpression of wri1-like gene from Brassica napus.

  2010cited by this paper
• Expression of ZmLEC1 and ZmWRI1 Increases Seed Oil Production in Maize1[W][OA]

  2010cited by this paper
• Analysis of Acyl Fluxes through Multiple Pathways of Triacylglycerol Synthesis in Developing Soybean Embryos1[W][OA]

  2009cited by this paper
• Oil accumulation in leaves directed by modification of fatty acid breakdown and lipid synthesis pathways.

  2009cited by this paper
• Use of metabolic control analysis to give quantitative information on control of lipid biosynthesis in the important oil crop, Elaeis guineensis (oilpalm).

  2009cited by this paper
• A leaf-based assay using interchangeable design principles to rapidly assemble multistep recombinant pathways.

  2009cited by this paper
• Welcome to the first issue of the seventh volume of the Plant Biotechnology Journal..

  2009cited by this paper
• Arabidopsis LEAFY COTYLEDON2 induces maturation traits and auxin activity: Implications for somatic embryogenesis

  2008cited by this paper
• Metabolic control analysis is helpful for informed genetic manipulation of oilseed rape (Brassica napus) to increase seed oil content

  2008cited by this paper
• WRINKLED1 specifies the regulatory action of LEAFY COTYLEDON2 towards fatty acid metabolism during seed maturation in Arabidopsis.

  2007cited by this paper
• Incorporation of Newly Synthesized Fatty Acids into Cytosolic Glycerolipids in Pea Leaves Occurs via Acyl Editing*

  2007cited by this paper
• LEAFY COTYLEDON 2 activation is sufficient to trigger the accumulation of oil and seed specific mRNAs in Arabidopsis leaves

  2005cited by this paper
• Mutation of the TGD1 Chloroplast Envelope Protein Affects Phosphatidate Metabolism in Arabidopsisw⃞

  2005cited by this paper
• Increased fatty acid production in potato by engineering of acetyl-CoA carboxylase

  2004cited by this paper
• WRINKLED1 encodes an AP2/EREB domain protein involved in the control of storage compound biosynthesis in Arabidopsis.

  2004cited by this paper
• An enhanced transient expression system in plants based on suppression of gene silencing by the p19 protein of tomato bushy stunt virus.

  2003cited by this paper
• Control analysis of lipid biosynthesis in tissue cultures from oil crops shows that flux control is shared between fatty acid synthesis and lipid assembly.

  2002cited by this paper
• Contrapuntal Networks of Gene Expression during Arabidopsis Seed Filling Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.000877.

  2002cited by this paper
• Chloroplast transformation with modified accD operon increases acetyl-CoA carboxylase and causes extension of leaf longevity and increase in seed yield in tobacco.

  2002cited by this paper
• LEAFY COTYLEDON2 encodes a B3 domain transcription factor that induces embryo development

  2001cited by this paper
• Seed-specific over-expression of an Arabidopsis cDNA encoding a diacylglycerol acyltransferase enhances seed oil content and seed weight.

  2001cited by this paper
• Expression in yeast and tobacco of plant cDNAs encoding acyl CoA:diacylglycerol acyltransferase.

  2000cited by this paper
• 1,2-sn-Diacylglycerol in plant cells: Product, substrate and regulator.

  1999cited by this paper
• The Arabidopsis thaliana TAG1 mutant has a mutation in a diacylglycerol acyltransferase gene.

  1999cited by this paper
• Arabidopsis LEAFY COTYLEDON1 is sufficient to induce embryo development in vegetative cells.

  1998cited by this paper
• wrinkled1: A novel, low-seed-oil mutant of Arabidopsis with a deficiency in the seed-specific regulation of carbohydrate metabolism.

  1998cited by this paper
• On The Bayesian Bootstrap

  1992cited by this paper
• Biosynthesis of complex lipids.

  1961cited by this paper
• Background Scope

  year unknowncited by this paper

• Powdery mildew induces chloroplast storage lipid formation at the expense of host thylakoids to promote spore production

  2025cites this paper
• Metabolic engineering of vegetative organs for enhanced oil accumulation in biofuel feedstocks

  2025cites this paper
• Water stress enhances triacylglycerol accumulation via different mechanisms in wild-type and transgenic high-leaf oil tobacco

  2025cites this paper
• Enhancing Oil Content in Oilseed Crops: Genetic Insights, Molecular Mechanisms, and Breeding Approaches

  2025cites this paper
• A comprehensive evaluation of metabolically engineered Cynara cardunculus calli as platform for valuable fatty acid derivatives production

  2025cites this paper
• Cell wall invertase improves grain nutrition via regulating sugar and hormone metabolism gene expression in transgenic soybean.

  2025cites this paper
• Cell death‐inducing DNA fragmentation factor alpha (DFFA)‐like effectors (CIDEs) improve lipid droplet (LD) formation and oil accumulation in plant tissues

  2025cites this paper
• Enhanced seed oil content by overexpressing LPAAT and WRI1 genes in tobacco plant

  2025cites this paper
• Unravelling the molecular regulation network of carbon metabolism and lipid metabolism during seed development in Akebia trifoliata via integrated multi-omics analysis

  2024cites this paper
• PfbZIP85 Transcription Factor Mediates ω-3 Fatty Acid-Enriched Oil Biosynthesis by Down-Regulating PfLPAT1B Gene Expression in Plant Tissues

  2024cites this paper
• Multiple transcription factors of Arabidopsis thaliana that are activated by LEAFY COTYLEDON 2 regulate triacylglycerol biosynthesis.

  2024cites this paper
• Exploration of advanced omics tools and resources for the improvement of industrial oil crops.

  2024cites this paper
• Research progress on genetic basis and QTL mapping of oil content in peanut seed

  2024influential citation
• Increasing oil content in Brassica oilseed species.

  2024cites this paper
• Metabolic engineering‐induced transcriptome reprogramming of lipid biosynthesis enhances oil composition in oat

  2024cites this paper
• Unveiling the camelina MBOAT gene family: Phylogenetic insights and regulatory landscape.

  2024cites this paper
• Exploiting lipid droplet metabolic pathway to foster lipid production: oleosin in focus

  2024cites this paper
• Functional Analysis of the Extraplastidial TRX System in Germination and Early Stages of Development of Arabidopsis thaliana.

  2024cites this paper
• Identification and Candidate Gene Evaluation of a Large Fast Neutron-Induced Deletion Associated with a High-Oil Phenotype in Soybean Seeds

  2024cites this paper
• Intron-mediated enhancement of DIACYLGLYCEROL ACYLTRANSFERASE1 expression in energycane promotes a step change for lipid accumulation in vegetative tissues

  2023cites this paper
• Genome-wide comprehensive characterization and Transcriptomic analysis of AP2/ERF gene family revealed its role in seed oil and ALA formation in Perilla (Perilla frutescens).

  2023cites this paper
• Metabolic Control Analysis of triacylglycerol accumulation in oilseed rape

  2023cites this paper
• Combined analysis of the metabolome and transcriptome provides insight into seed oil accumulation in soybean

  2023cites this paper
• Polyunsaturated Fatty Acids: Conversion to Lipid Mediators, Roles in Inflammatory Diseases and Dietary Sources

  2023cites this paper
• Progress in understanding and improving oil content and quality in seeds

  2023cites this paper
• Modular Metabolic Engineering of Mortierella alpina by the 2A Peptide Platform to Improve Arachidonic Acid Production.

  2023cites this paper
• Acyl-CoA-dependent and acyl-CoA-independent avocado acyltransferases positively influence oleic acid content in nonseed triacylglycerols

  2023cites this paper
• Ectopic Expression of Perilla frutescens WRI1 Enhanced Storage Oil Accumulation in Nicotiana benthamiana Leaves

  2023cites this paper
• Powdery mildew infection induces a non-canonical route to storage lipid formation at the expense of host thylakoid lipids to fuel its spore production

  2023cites this paper
• Dynamic Regulation of Lipid Droplet Biogenesis in Plant Cells and Proteins Involved in the Process

  2023cites this paper
• Expression of malic enzyme reveals subcellular carbon partitioning for storage reserve production in soybeans.

  2023cites this paper
• Triacylglycerol, total fatty acid, and biomass accumulation of metabolically engineered energycane grown under field conditions confirms its potential as feedstock for drop‐in fuel production

  2023cites this paper
• Transcriptional regulation of oil biosynthesis in seed plants: Current understanding, applications, and perspectives

  2022cites this paper
• Overexpression of Soybean GmWRI1a Stably Increases the Seed Oil Content in Soybean

  2022cites this paper
• Acyl-CoA:diacylglycerol acyltransferase: Properties, physiological roles, metabolic engineering and intentional control.

  2022cites this paper
• Sunflower WRINKLED1 Plays a Key Role in Transcriptional Regulation of Oil Biosynthesis

  2022cites this paper
• Challenges and prospects for sustainable microalga-based oil: A comprehensive review, with a focus on metabolic and genetic engineering

  2022cites this paper
• Energy plants (crops): potential natural and future designer plants

  2022cites this paper
• Engineering triacylglycerol accumulation in duckweed (Lemna japonica)

  2022cites this paper
• Pyruvate transporter BnaBASS2 impacts seed oil accumulation in Brassica napus

  2022cites this paper
• Genome-wide identification and functional analysis of Dof transcription factor family in Camelina sativa

  2022cites this paper
• Comparative Genomic Analysis Uncovers the Chloroplast Genome Variation and Phylogenetic Relationships of Camellia Species

  2022cites this paper
• BnaNTT2 regulates ATP homeostasis in plastid to sustain lipid metabolism and plant growth in Brassica napus

  2022cites this paper
• Towards oilcane: preliminary field evaluation of metabolically engineered sugarcane with hyper-accumulation of triacylglycerol in vegetative tissues

  2022cites this paper
• Improving crop yield potential: Underlying biological processes and future prospects

  2022cites this paper
• Is CRISPR/Cas9 a way forward to fast-track genetic improvement in commercial palms? Prospects and limits

  2022cites this paper
• Expression of AtWRI1 and AtDGAT1 during soybean embryo development influences oil and carbohydrate metabolism

  2022influential citation
• BnaPPT1 is essential for chloroplast development and seed oil accumulation in Brassica napus

  2022cites this paper
• Molecular basis of the key regulator WRINKLED1 in plant oil biosynthesis

  2022cites this paper
• Metabolic engineering of energycane to hyperaccumulate lipids in vegetative biomass

  2022cites this paper
• Nontargeted metabolomic and multigene expression analyses reveal the mechanism of oil biosynthesis in sea buckthorn berry pulp rich in palmitoleic acid.

  2021cites this paper
• Metabolic flux analysis of the non-transitory starch tradeoff for lipid production in mature tobacco leaves

  2021cites this paper
• Comparison of quantitative trait loci (QTLs) associated with yield components in two commercial Dura × Pisifera breeding crosses

  2021cites this paper
• Fettsäuren und Fettsäurederivate als nachwachsende Plattformmoleküle für die chemische Industrie

  2021cites this paper
• A transcriptional journey from sucrose to endosperm oil bodies in triple transgene oily wheat grain

  2021cites this paper
• A review on co-culturing of microalgae: A greener strategy towards sustainable biofuels production.

  2021cites this paper
• Function of ORFC of the polyketide synthase gene cluster on fatty acid accumulation in Schizochytrium limacinum SR21

  2021cites this paper
• Sesamum indicum Oleosin L improves oil packaging in Nicotiana benthamiana leaves

  2021cites this paper
• Fatty Acids and their Derivatives as Renewable Platform Molecules for the Chemical Industry

  2021cites this paper
• Ectopic Expression of OLEOSIN 1 and Inactivation of GBSS1 Have a Synergistic Effect on Oil Accumulation in Plant Leaves

  2021cites this paper
• Seed oil storage in three contrasted legume species: implications for oil improvement

  2020cites this paper
• Molecular Basis of Plant Oil Biosynthesis: Insights Gained From Studying the WRINKLED1 Transcription Factor

  2020cites this paper
• The function of the WRI1-TCP4 regulatory module in lipid biosynthesis

  2020cites this paper
• Producing Cyclopropane Fatty Acid in Plant Leafy Biomass via Expression of Bacterial and Plant Cyclopropane Fatty Acid Synthases

  2020cites this paper
• Towards model-driven characterization and manipulation of plant lipid metabolism.

  2020cites this paper
• Research advances of WRINKLED1 (WRI1) in plants.

  2020cites this paper
• Functional characterization of two type-1 diacylglycerol acyltransferase (DGAT1) genes from rice (Oryza sativa) embryo restoring the triacylglycerol accumulation in yeast

  2020cites this paper
• Working with Randy: The Diacylglycerol Acyltransferase Story.

  2020cites this paper
• A Synergistic Genetic Engineering Strategy Induced Triacylglycerol Accumulation in Potato (Solanum tuberosum) Leaf

  2020cites this paper
• Sorghum bicolor cultivars have divergent and dynamic gene regulatory networks that control the temporal expression of genes in stem tissue

  2020cites this paper
• AtMYB92 enhances fatty acid synthesis and suberin deposition in leaves of Nicotiana benthamiana.

  2020cites this paper
• Engineering trienoic fatty acids into cottonseed oil improves low-temperature seed germination, plant photosynthesis and cotton fibre quality.

  2020cites this paper
• TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTOR4 Interacts with WRINKLED1 to Mediate Seed Oil Biosynthesis1[OPEN]

  2020cites this paper
• Towards oilcane: Engineering hyperaccumulation of triacylglycerol into sugarcane stems

  2020influential citation
• Application of transgenic technologies in biofuel production through photosynthetic chassis—new paradigms from gene mining to genome editing

  2020cites this paper
• Characterization of the TCP4-WRI1 complex regulating Arabidopsis seed oil biosynthesis

  2020cites this paper
• Embryo development and the oil and protein bodies in Medicago truncatula

  2020cites this paper
• Recent advances and future directions in plant and yeast engineering to improve lignocellulosic biofuel production

  2020cites this paper
• Identification and functional characterization of two acyl CoA:diacylglycerol acyltransferase 1 (DGAT1) genes from forage sorghum (Sorghum bicolor) embryo.

  2020cites this paper
• Molecular and biochemical analysis of the castor caruncle reveals a set of unique genes involved in oil accumulation in non-seed tissues

  2019cites this paper
• FIELD EVALUATION OF TOBACCO ENGINEERED FOR HIGH LEAF-OIL ACCUMULATION

  2019influential citation
• Kinetic improvement of an algal diacylglycerol acyltransferase 1 via fusion with an acyl-CoA binding protein

  2019cites this paper
• Increase in lysophosphatidate acyltransferase activity in oilseed rape (Brassica napus) increases seed triacylglycerol content despite its low intrinsic flux control coefficient

  2019cites this paper
• Identification and function analysis of a type 2 diacylglycerol acyltransferase (DGAT2) from the endosperm of coconut (Cocos nucifera L.).

  2019cites this paper
• Co-culturing of oleaginous microalgae and yeast: paradigm shift towards enhanced lipid productivity

  2019cites this paper
• RNA-seq data reveals a coordinated regulation mechanism of multigenes involved in the high accumulation of palmitoleic acid and oil in sea buckthorn berry pulp

  2019cites this paper
• Recent advances in enhancement of oil content in oilseed crops.

  2019cites this paper
• Metabolic engineering for enhanced oil in biomass.

  2019cites this paper
• Cellular Organization and Regulation of Plant Glycerolipid Metabolism.

  2019cites this paper
• Expression of the Arabidopsis WRINKLED 1 transcription factor leads to higher accumulation of palmitate in soybean seed

  2019cites this paper
• Identification of Genes Involved in Lipid Biosynthesis through de novo Transcriptome Assembly from Cocos nucifera Developing Endosperm

  2019cites this paper
• WRINKLED1, a “Master Regulator” in Transcriptional Control of Plant Oil Biosynthesis

  2019cites this paper
• A Versatile High Throughput Screening Platform for Plant Metabolic Engineering Highlights the Major Role of ABI3 in Lipid Metabolism Regulation

  2019cites this paper
• Comparative transcriptomic analysis of high- and low-oil Camellia oleifera reveals a coordinated mechanism for the regulation of upstream and downstream multigenes for high oleic acid accumulation

  2019cites this paper
• Metabolic engineering of lipid pathways in Saccharomyces cerevisiae and staged bioprocess for enhanced lipid production and cellular physiology

  2018cites this paper
• WRINKLED1 transcription factor: How much do we know about its regulatory mechanism?

  2018cites this paper
• Characterization and engineering of a dual-function diacylglycerol acyltransferase in the oleaginous marine diatom Phaeodactylum tricornutum

  2018cites this paper
• WRINKLED1 and ACYL-COA:DIACYLGLYCEROL ACYLTRANSFERASE1 regulate tocochromanol metabolism in Arabidopsis.

  2018cites this paper
• Integrated soil-cotton system management enhances triacylglycerol yield and favourable fatty acid profile

  2018cites this paper
• Characterization of Acyltransferases and WRINKLED Orthologs Involved in TAG Biosynthesis in Avocado

  2018influential citation