¹ ³C labeling unveils soil and microbial changes in continuously cropped Pogostemon cablin.

Continuous cropping (CC) disrupts agriculture by causing soil degradation, yield decline, and microbial imbalances due to nutrient loss and pathogen buildup. However, the mechanisms underlying these impacts on Pogostemon cablin remain unclear. A greenhouse pot experiment was conducted during the rapid growth stage when CC-induced stress is most pronounced to address this gap. The ¹ ³C stable isotope labeling was employed to trace microbial shifts and decomposition of organic matter within the plant-soil-microbe interface. The results indicated that CC significantly alleviated plant growth and soil multifunctional index. Specifically, it enhanced the levels of available phosphorus (AP), available potassium (AK), total nitrogen (TN), and soil organic carbon (SOC) while leading to a decline in pH, water holding capacity (WHC), and total potassium (TK) over the years. While ¹ ³C-labeled bacteriome under CC obstacles positively influenced microbial diversity and community composition, highlighting the pivotal role of a diverse microbial community in promoting ecosystem resilience, nutrient cycling, disease suppression, and overall soil health. Specific taxa, including Escherichia_coli, Comamonadaceae, Planococcaceae, and Gaiella, were identified as key contributors to these improvements. Additionally, Mantel test analyses indicated that Aneurinibacillus, Actinomadura, E._coli, Acinetobacter, and Moraxellaceae enhanced soil multifunctionality. Functional predictions using FAPROTAX revealed increased microbial processes such as nitrogen (N) fixation, aromatic compound degradation, animal parasites or symbionts, N respiration, and nitrate respiration under ¹ ³C-labeled conditions. These findings emphasize the inter-annual variations in rhizosphere bacteriomes under CC and demonstrate the effectiveness of ¹ ³C labeling in elucidating C turnover and microbial interactions. This study provides actionable insights for mitigating CC-induced soil challenges and advancing sustainable agricultural management.

• Publication year

  2025

• Venue

  Ecotoxicology and Environmental Safety

• Publication date

  2025-08-27

• Fields of study

  Agricultural and Food Sciences, Medicine, Environmental Science

• Identifiers
  DOI 10.1016/j.ecoenv.2025.118940PMID 40876187
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Continuous cropping of Patchouli alters soil physiochemical properties and rhizosphere microecology revealed by metagenomic sequencing

  2025cited by this paper
• Self-alleviation of continuous-cropping obstacles in potato via root-exudate-driven recruitment of growth-promoting bacteria

  2025cited by this paper
• Impacts of continuous potato cropping on soil microbial assembly processes and spread of potato common scab

  2025cited by this paper
• Iron-nitrogen coupling mediates soil microbial community composition and gene expression during the formation of rusty root in Panax ginseng from three northeast provinces in China

  2024cited by this paper
• Human activities strengthen the influence of deterministic processes in the mechanisms of fish community assembly in tropical rivers of Yunnan, China.

  2024cited by this paper
• DNA stable isotope probing reveals the impact of trophic interactions on bioaugmentation of soils with different pollution histories

  2024cited by this paper
• Metabolic activity of gut microbial enrichment cultures from different marine species and their transformation abilities to plastic additives.

  2024cited by this paper
• Core microbial taxonomies that maintain high organic carbon content in upland soil.

  2024cited by this paper
• Continuous cropping system altered soil microbial communities and nutrient cycles

  2024cited by this paper
• Ralstonia solanacearum differentially modulates soil physicochemical properties and rhizospheric bacteriome of resistant and susceptible tobacco cultivars.

  2024cited by this paper
• Pigmentiphaga kullae CHJ604 improved the growth of tobacco by degrading allelochemicals and xenobiotics in continuous cropping obstacles.

  2024cited by this paper
• Continuous Cropping of Patchouli Alleviate Soil Properties, Enzyme Activities, and Bacterial Community Structures

  2024cited by this paper
• Long–term rotational and perennial cropping benefit soil organic carbon stocks and ecosystem multifunctionality

  2024cited by this paper
• pH is the major predictor of soil microbial network complexity in Chinese forests along a latitudinal gradient

  2024cited by this paper
• Organic Farming Enhances Diversity and Recruits Beneficial Soil Fungal Groups in Traditional Banana Plantations

  2024cited by this paper
• Seed coat treatment by plant-growth-promoting rhizobacteria Lysobacter antibioticus 13–6 enhances maize yield and changes rhizosphere bacterial communities

  2023cited by this paper
• Microbiome resistance mediates stimulation of reduced graphene oxide to simultaneous abatement of 2,2',4,4',5-pentabromodiphenyl ether and 3,4-dichloroaniline in paddy soils.

  2023cited by this paper
• Identification of the CDPK gene family in patchouli and functional analysis in response to continuous cropping stress

  2023cited by this paper
• Continuous crop rotation increases soil organic carbon stocks in river deltas: A 40-year field evidence.

  2023cited by this paper
• Using biochar for the treatment of continuous cropping obstacle of herbal remedies: A review

  2023cited by this paper
• Bibliometric Analysis And Visualization: A Development Of Patchouli (Pogostemon Cablin) Related Research From 2013-2023

  2023cited by this paper
• Effects of 13C isotope-labeled allelochemicals on the growth of the invasive plant Alternanthera philoxeroides

  2023cited by this paper
• Effect of Growth-promoting Bacterial Consortia on Overall Growth of Tomato Plants

  2023cited by this paper
• A Systematic Review on the Continuous Cropping Obstacles and Control Strategies in Medicinal Plants

  2023cited by this paper
• Gut bacterial community and gene expression alterations induced by transgenic Bt maize contribute to insecticidal activity against Mythimna separata

  2023cited by this paper
• Responses of Soil Microbial Communities in Soybean–Maize Rotation to Different Fertilization Treatments

  2023cited by this paper
• Tracing service crops' net carbon and nitrogen rhizodeposition into soil organic matter fractions using dual isotopic brush-labeling

  2023cited by this paper
• Different changes of bacterial diversity and soil metabolites in tea plants-legume intercropping systems

  2023cited by this paper
• Host genetics regulate the plant microbiome.

  2023cited by this paper
• Changes in litter input exert divergent effects on the soil microbial community and function in stands of different densities.

  2022cited by this paper
• Soybean continuous cropping affects yield by changing soil chemical properties and microbial community richness

  2022cited by this paper
• Soil structure and microbiome functions in agroecosystems

  2022cited by this paper
• Combined physiological and transcriptome analysis revealed the response mechanism of Pogostemon cablin roots to p-hydroxybenzoic acid

  2022cited by this paper
• Overfertilization reduces tomato yield under long-term continuous cropping system via regulation of soil microbial community composition

  2022cited by this paper
• Responses of photosynthetic characteristics and leaf senescence in summer maize to simultaneous stresses of waterlogging and shading

  2022cited by this paper
• Soil Microbial Network Complexity Varies With pH as a Continuum, Not a Threshold, Across the North China Plain

  2022cited by this paper
• Microbial functional assemblages predicted by the FAPROTAX analysis are impacted by physicochemical properties, but C, N and S cycling genes are not in mangrove soil in the Beibu Gulf, China

  2022cited by this paper
• Temporal variation of management effects on soil microbial communities

  2022cited by this paper
• Differences in the nitrous oxide emission and the nitrifier and denitrifier communities among varying aggregate sizes of an arable soil in China

  2021cited by this paper
• In-situ 13CO2 labeling to trace carbon fluxes in plant-soil-microorganism systems: Review and methodological guideline

  2021cited by this paper
• Indirect reduction of Ralstonia solanacearum via pathogen helper inhibition

  2021cited by this paper
• Automation of QIIME2 Metagenomic Analysis Platform

  2021cited by this paper
• Transcriptome analysis of two Pogostemon cablin chemotypes reveals genes related to patchouli alcohol biosynthesis

  2021cited by this paper
• Linking microbial taxa and the effect of mineral nitrogen forms on residue decomposition at the early stage in arable soil by DNA-qSIP

  2021cited by this paper
• Isopentenyl Diphosphate Isomerase (IPI) Gene Silencing Negatively Affects Patchouli Alcohol Biosynthesis in Pogostemon cablin

  2021cited by this paper
• Temporal patterns of carbon flow from grassland vegetation to soil microorganisms measured using 13C-labelling and signature fatty acids

  2021cited by this paper
• Variations of rhizospheric soil microbial communities in response to continuous Andrographis paniculata cropping practices

  2020cited by this paper
• Long-term continuous cropping of soybean is comparable to crop rotation in mediating microbial abundance, diversity and community composition

  2020cited by this paper
• A comprehensive analysis of the response of the fungal community structure to long-term continuous cropping in three typical upland crops

  2020cited by this paper
• Preparation of uniformly labelled 13C- and 15N-plants using customised growth chambers

  2020cited by this paper
• Harnessing rhizosphere microbiomes for drought-resilient crop production

  2020cited by this paper
• Isotope applications to soil science at the University of Alberta — an historical perspective1

  2020cited by this paper
• Phase changes of continuous cropping obstacles in strawberry (Fragaria × ananassa Duch.) production

  2020cited by this paper
• Plant–microbiome interactions: from community assembly to plant health

  2020cited by this paper
• Intercropping With Turmeric or Ginger Reduce the Continuous Cropping Obstacles That Affect Pogostemon cablin (Patchouli)

  2020cited by this paper
• Hormones as go‐betweens in plant microbiome assembly

  2020cited by this paper
• Soil Aggregate Microbial Communities: Towards Understanding Microbiome Interactions at Biologically Relevant Scales

  2019cited by this paper
• From Functional Potential of Soil Bacterial Communities Towards Petroleum Hydrocarbons Bioremediation

  2019cited by this paper
• Interactions between elevated CO2 levels and floating aquatic plants on the alteration of bacterial function in carbon assimilation and decomposition in eutrophic waters.

  2019cited by this paper
• Contamination in Low Microbial Biomass Microbiome Studies: Issues and Recommendations.

  2019cited by this paper
• Variation of 13C and 15N enrichments in different plant components of labeled winter wheat (Triticum aestivum L.)

  2019cited by this paper
• Sensitivity of Nematode Community Analysis to Agricultural Management Practices and Inoculation with Local Effective Microorganisms in the Southeastern United States

  2019cited by this paper
• Predicting the evolution of Escherichia coli by a data-driven approach

  2018cited by this paper
• Field 13C Pulse Labeling of Pea, Wheat, and Vetch Plants for Subsequent Root and Shoot Decomposition Studies

  2018cited by this paper
• The essential oil of patchouli, Pogostemon cablin: A review

  2018cited by this paper
• Mechanisms by which organic fertilizer and effective microbes mitigate peanut continuous cropping yield constraints in a red soil of south China

  2018cited by this paper
• Nitrification in agricultural soils: impact, actors and mitigation.

  2018cited by this paper
• Heterogeneity of interactions of microbial communities in regions of Taihu Lake with different nutrient loadings: A network analysis

  2018cited by this paper
• A 13C isotope labeling method for the measurement of lignin metabolic flux in Arabidopsis stems

  2018cited by this paper
• fastp: an ultra-fast all-in-one FASTQ preprocessor

  2018cited by this paper
• DADA2: High resolution sample inference from Illumina amplicon data

  2016cited by this paper
• Reverse and Multiple Stable Isotope Probing to Study Bacterial Metabolism and Interactions at the Single Cell Level.

  2016cited by this paper
• The minimal rhizosphere microbiome

  2015cited by this paper
• Hydrocarbon degradation by a newly isolated thermophilic Anoxybacillus sp. with bioemulsifier production and new alkB genes

  2015cited by this paper
• Improved Bacterial 16S rRNA Gene (V4 and V4-5) and Fungal Internal Transcribed Spacer Marker Gene Primers for Microbial Community Surveys

  2015cited by this paper
• Community structure and soil pH determine chemoautotrophic carbon dioxide fixation in drained paddy soils.

  2015cited by this paper
• A new simple method for labeling field crops with stable isotope tracers

  2014cited by this paper
• Design and Operation of a Continuous 13C and 15N Labeling Chamber for Uniform or Differential, Metabolic and Structural, Plant Isotope Labeling

  2014cited by this paper
• Spatial Ecology of Bacteria at the Microscale in Soil

  2014cited by this paper
• Roots and Associated Fungi Drive Long-Term Carbon Sequestration in Boreal Forest

  2013cited by this paper
• UPARSE: highly accurate OTU sequences from microbial amplicon reads

  2013cited by this paper
• Soil organic matter dynamics: a biological perspective derived from the use of compound-specific isotopes studies

  2013cited by this paper
• Effect of grazing on carbon stocks and assimilate partitioning in a Tibetan montane pasture revealed by 13CO2 pulse labeling

  2012cited by this paper
• The rhizosphere microbiome and plant health.

  2012cited by this paper
• The 15N isotope effect in Escherichia coli: A neutron can make the difference

  2012cited by this paper
• Tracking Stable Isotope Enrichment in Tree Seedlings with Solid-State NMR Spectroscopy

  2012cited by this paper
• Quality-filtering vastly improves diversity estimates from Illumina amplicon sequencing

  2012cited by this paper
• Metagenomic biomarker discovery and explanation

  2011cited by this paper
• Deciphering the Rhizosphere Microbiome for Disease-Suppressive Bacteria

  2011cited by this paper
• Emerging molecular insights into the interaction between probiotics and the host intestinal mucosa

  2011cited by this paper
• Identifying the characteristics of organic soil amendments that suppress soilborne plant diseases.

  2010cited by this paper
• Partitioning pattern of carbon flux in a Kobresia grassland on the Qinghai‐Tibetan Plateau revealed by field 13C pulse‐labeling

  2009cited by this paper
• Acinetobacter baumannii: Emergence of a Successful Pathogen

  2008cited by this paper
• DNA stable-isotope probing

  2007cited by this paper
• Dynamics of Microbial Communities during Decomposition of Carbon-13 Labeled Ryegrass Fractions in Soil

  2005cited by this paper
• In situ13CO2 pulse-labelling of upland grassland demonstrates a rapid pathway of carbon flux from arbuscular mycorrhizal mycelia to the soil

  2002cited by this paper
• CARBON CYCLING AND SOIL CARBON STORAGE IN MESIC TO WET HAWAIIAN MONTANE FORESTS

  2001cited by this paper
• Changes in Forest Biomass Carbon Storage in China Between 1949 and 1998

  2001cited by this paper
• A pulse-labelling method to generate 13C- enriched plant materials

  2001cited by this paper
• Carbon partitioning and below-ground translocation by Lolium perenne

  2001cited by this paper

• No citing papers are available for this paper.