Insulin-like growth factor I receptors in neuronal and glial cells. Characterization and biological effects in primary culture.

Primary cultures of neuronal and glial cells from 1-day-old neonatal rats contain high affinity receptors for insulin-like growth factor I (IGF-I). The IC50 for displacement of 125I-IGF-I binding by unlabeled IGF-I was 3 nM for neuronal cells and 4 nM for glial cells. Unlabeled insulin was 20-50 times less potent. Apparent molecular mass of the alpha subunits of the IGF-I receptor was 125 kDa in neuronal and 135 kDa in glial cells. IGF-I induced autophosphorylation of the IGF-I receptor beta subunit in lectin-purified membrane preparations in a dose-dependent manner. The major phosphoamino acid of the beta subunit in both cell types was tyrosine in the IGF-I-stimulated state and serine in the basal state. Apparent molecular mass of the beta subunits of the IGF-I receptors was 91 kDa for neuronal and 95 kDa for glial cells. Tyrosine kinase activity of the IGF-I receptors was demonstrated by IGF-I-induced phosphorylation of the exogenous substrate poly(Glu, Tyr) 4:1 in both cell types. IGF-I had no effect on 2-deoxyglucose uptake in neuronal cells. In contrast, in glial cells, IGF-I stimulated 2-deoxyglucose uptake at very high doses, presumably acting via the insulin receptor. The effect of IGF-I as a neurotrophic growth factor in both neuronal and glial cells was demonstrated by its stimulation of [3H]thymidine incorporation. These findings suggest the IGF-I is an important growth factor in nervous tissue-derived cells.

• Publication year

  1987

• Venue

  Journal of Biological Chemistry

• Publication date

  1987-06-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/s0021-9258(18)47623-5PMID 2953724
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Characterization of a neuronal subtype of insulin-like growth factor I receptor.

  1987cited by this paper
• Development of brain insulin receptors: structural and functional studies of insulin receptors from whole brain and primary cell cultures.

  1986cited by this paper
• Insulin-like growth factor I/somatomedin C: a potent inducer of oligodendrocyte development.

  1986cited by this paper
• Insulin‐like growth factor I receptor primary structure: comparison with insulin receptor suggests structural determinants that define functional specificity.

  1986cited by this paper
• Tyrosine kinase activity of brain insulin and IGF-1 receptors.

  1986cited by this paper
• Binding and Internalization of Insulin and Insulin-Like Growth Factors by Isolated Brain Microvessels

  1986cited by this paper
• Insulin receptors in lizard brain and liver: structural and functional studies of alpha and beta subunits demonstrate evolutionary conservation.

  1986cited by this paper
• Purification and characterization of the receptor for insulin-like growth factor I.

  1986cited by this paper
• Autoradiographic localization of insulin receptors in rat brain: prominence in olfactory and limbic areas.

  1986cited by this paper
• Purification of the type I insulin-like growth factor receptor from human placenta.

  1986cited by this paper
• Insulin-like growth factor II (IGF II) in human brain: regional distribution of IGF II and of higher molecular mass forms.

  1985cited by this paper
• The nature and regulation of the receptors for insulin-like growth factors.

  1985cited by this paper
• Characterization of insulin-like growth factor I-stimulated tyrosine kinase activity associated with the beta-subunit of type I insulin-like growth factor receptors of rat liver cells.

  1985cited by this paper
• Insulin receptors and insulin modulation of norepinephrine uptake in neuronal cultures from rat brain.

  1985cited by this paper
• Two types of receptor for insulin‐like growth factors in mammalian brain.

  1985cited by this paper
• Insulin-like growth factor-I (IGF-I) stimulates tyrosine kinase activity in purified receptors from a rat liver cell line.

  1984cited by this paper
• Characterization of insulin-like growth factor receptors in rat anterior pituitary, hypothalamus, and brain.

  1984cited by this paper
• The insulin receptor of rat brain is coupled to tyrosine kinase activity.

  1984cited by this paper
• Characterization of high affinity receptors for insulin-like growth factors I and II on rat anterior pituitary cells.

  1984cited by this paper
• Insulin binds to specific receptors and stimulates 2-deoxy-D-glucose uptake in cultured glial cells from rat brain.

  1984cited by this paper
• Unique Features of the Insulin Receptor in Rat Brain

  1984cited by this paper
• Somatomedin-C stimulates the phosphorylation of the beta-subunit of its own receptor.

  1983cited by this paper
• Stimulation of tyrosine-specific phosphorylation in vitro by insulin-like growth factor I

  1983cited by this paper
• Characterization of insulin-mediated phosphorylation of the insulin receptor in a cell-free system.

  1983cited by this paper
• Structural differences between insulin receptors in the brain and peripheral target tissues.

  1983cited by this paper
• Detection and quantification of phosphotyrosine in proteins.

  1983cited by this paper
• Binding of insulin receptors to lectins: evidence for common carbohydrate determinants on several membrane receptors.

  1981cited by this paper
• Purification of the insulin receptor from human placenta by chromatography on immobilized wheat germ lectin and receptor antibody.

  1980cited by this paper
• Characterization of insulin receptor subunits in brain and other tissues by photoaffinity labeling.

  1980cited by this paper
• Binding of [125I]insulin to specific receptors and stimulation of nucleotide incorporation in cells cultured from rat brain.

  1980cited by this paper
• Insulin receptors are widely distributed in the central nervous system of the rat

  1978cited by this paper

• GC-MS SCRUTINY OF PHYTOCONSTITUENTS, IN VITRO EVALUATION OF ANTIHYPERGLYCEMIC, ANTIADIPOGENIC ACTIVITIES, AND CYTOTOXIC EFFECT USING 3T3 L1 ADIPOCYTE CELL LINE AND MOLECULAR DOCKING STUDIES OF PREMNA CORYMBOSA

  2024cites this paper
• Impact of Growth Hormone on Microglial and Astrocytic Function.

  2024cites this paper
• Growth Hormone, Hypothalamic Inflammation, and Aging

  2024cites this paper
• Neurotrophic Therapy for ALS/MND

  2021cites this paper
• rhIGF‐1 reduces the permeability of the blood‐brain barrier following intracerebral hemorrhage in mice

  2019cites this paper
• IGFBP-2 Signaling in the Brain: From Brain Development to Higher Order Brain Functions

  2019cites this paper
• To Be or Not to Be: Environmental Factors that Drive Myelin Formation during Development and after CNS Trauma

  2018cites this paper
• Insulin-Like Growth Factor (IGF)-I Modulates Endothelial Blood-Brain Barrier Function in Ischemic Middle-Aged Female Rats.

  2016cites this paper
• Insulin-like signaling (IIS) responses to temperature, genetic background, and growth variation in garter snakes with divergent life histories.

  2016cites this paper
• Effects of high fat diet on cognition and brain pathology

  2015cites this paper
• Growth hormone, insulin-like growth factor-1 and the aging brain.

  2015cites this paper
• Insulin and insulin-like growth factor receptors in the brain: physiological and pathological aspects.

  2014cites this paper
• Brain Alterations and Clinical Symptoms of Dementia in Diabetes: Aβ/Tau-Dependent and Independent Mechanisms

  2014cites this paper
• Roles of vascular and metabolic components in cognitive dysfunction of Alzheimer disease: short- and long-term modification by non-genetic risk factors

  2013cites this paper
• Aβ Deposition, Insulin Signaling, and Tau Phosphorylation in Animal Models of Alzheimer Disease and Diabetes

  2013cites this paper
• Novel Approaches to the Treatment of Alzheimer’s Disease

  2012cites this paper
• Effect of growth factors, steroids, alfa-lipoic acid and Astroglial Conditioned Media on the expression of some biomarkers of astroglial cell proliferation and differentiation in primary culture

  2012cites this paper
• Insulin, insulin-like growth factor–1, insulin receptor, and insulin-like growth factor–1 receptor expression in the chick eye and their regulation with imposed myopic or hyperopic defocus

  2011cites this paper
• Defects in IGF-1 receptor, insulin receptor and IRS-1/2 in Alzheimer's disease indicate possible resistance to IGF-1 and insulin signalling.

  2010cites this paper
• Neurotrophic Growth Factors for the Treatment of Amyotrophic Lateral Sclerosis: Where Do We Stand?

  2010cites this paper
• Molecular mechanisms involved in insulin-like growth factor I-and growth hormone-induced oligodendrocyte development

  2009cites this paper
• Insulin and insulin-like growth factor receptors in the nervous system

  2008cites this paper
• Insulin and IGF‐1 enhance the expression of the neuronal monocarboxylate transporter MCT2 by translational activation via stimulation of the phosphoinositide 3‐kinase–Akt–mammalian target of rapamycin pathway

  2007cites this paper
• Intracellular events mediating insulin‐like growth factor I‐induced oligodendrocyte development: modulation by cyclic AMP

  2005cites this paper
• Alcohol alters insulin-like growth factor-1 activated oct 2 POU domain gene expression in the immature female hypothalamus.

  2005cites this paper
• Growth Hormone, Insulin-Like Growth Factor-1, and the Biology of Aging

  2005cites this paper
• Physiology and Gene Expression Characteristics of Carcinogen‐Initiated and Tumor‐Transformed Glial Progenitor Cells Derived from the CNS of Methylnitrosourea (MNU)‐Treated Sprague‐Dawley Rats

  2004cites this paper
• Insulin‐like Growth Factor Binding Protein 2: Gene Expression Microarrays and the Hypothesis‐generation Paradigm

  2002cites this paper
• Insulin-like growth factor-I inhibits endogenous acetylcholine release from the rat hippocampal formation: possible involvement of GABA in mediating the effects.

  2002cites this paper
• Age-Related Decreases in Growth Hormone and Insulin-Like Growth Factor (IGF)–1: Implications for Brain Aging

  2001cites this paper
• Effects of voluntary ethanol drinking on [125I]insulin-like growth factor-I, [125I]insulin-like growth factor-II and [125I]insulin receptor binding in the mouse hippocampus and cerebellum.

  2000cites this paper
• Golf ball epilepsy

  2000cites this paper
• Insulin‐like growth factor‐I and its receptor in the frontal cortex, hippocampus, and cerebellum of normal human and Alzheimer disease brains

  2000cites this paper
• The Tyrosine Kinase Activity of the Chicken Insulin Receptor Is Similar to That of the Human Insulin Receptor

  2000cites this paper
• Insulin-like growth factor-1 (IGF-1): a neuroprotective trophic factor acting via the Akt kinase pathway.

  2000cites this paper
• Stimulation of brain hexokinase gene expression by recombinant brain insulin-like growth factor in C6 glial cells.

  1999cites this paper
• Alterations in insulin-like growth factor-1 gene and protein expression and type 1 insulin-like growth factor receptors in the brains of ageing rats.

  1999cites this paper
• Pleiotropic effects of growth hormone and insulin-like growth factor (IGF)-1 on biological aging: inferences from moderate caloric-restricted animals.

  1999cites this paper
• In vivo actions of fibroblast growth factor‐2 and insulin‐like growth factor‐I on oligodendrocyte development and myelination in the central nervous system

  1999cites this paper
• Regulation of brain glucose transporters by glucose and oxygen deprivation.

  1999cites this paper
• Insulin/insulin-like growth factor-I hybrid receptors with high affinity for insulin are developmentally regulated during neurogenesis.

  1999cites this paper
• Mucus of the human olfactory epithelium contains the insulin-like growth factor-I system which is altered in some neurodegenerative diseases.

  1999cites this paper
• Effects of [beta]-amyloid on glucose uptake by cultured hippocampal neurons

  1998cites this paper
• Thyroid hormone acting on astrocytes in culture

  1998cites this paper
• Induction of IGF-1 mRNA expression following traumatic injury to the postnatal brain.

  1998cites this paper
• Molecular Mechanisms of Insulin‐like Growth Factor I Receptor Function: Implications for Normal Physiology and Pathological States

  1998cites this paper
• Insulin-Like Growth Factor-I Is a Differentiation Factor for Postmitotic CNS Stem Cell-Derived Neuronal Precursors: Distinct Actions from Those of Brain-Derived Neurotrophic Factor

  1998cites this paper
• Reduced MK801 binding in neocortical neurons after AAV-mediated transfections with NMDA-R1 antisense cDNA.

  1998cites this paper
• Interaction in vitro of the product of the c-Crk-II proto-oncogene with the insulin-like growth factor I receptor.

  1998cites this paper
• Insulin-like growth factor-I-induced DNA synthesis in insulin-secreting cell line RINm5F is associated with phosphorylation of the insulin-like growth factor-I receptor and the insulin receptor substrate-2.

  1998cites this paper
• Localization of the insulin-like growth factor I receptor in the cerebellum and hypothalamus of adult rats: an electron microscopic study

  1997cites this paper
• Insulin-like growth factors and insulin stimulate erythropoietin production in primary cultured astrocytes.

  1997cites this paper
• Amyloid precursor protein requires the insulin signaling pathway for neurotrophic activity.

  1997cites this paper
• The effect of insulin-like growth factors on brain myelination and their potential therapeutic application in myelination disorders.

  1997cites this paper
• Localization of the insulin-like growth factor I receptor in the cerebellum and hypothalamus of adult rats: an electron microscopic study

  1997cites this paper
• Insulin-like growth factors in central nervous system tumors

  1997cites this paper
• Presence and differential internalization of two distinct insulin-like growth factor receptors in rat hippocampal neurons.

  1997cites this paper
• Insulin-like growth factors-I and -II differentially regulate endogenous acetylcholine release from the rat hippocampal formation.

  1997cites this paper
• Colocalisation of insulin and IGF‐1 receptors in cultured rat sensory and sympathetic ganglion cells

  1997cites this paper
• Systemic administration of kainic acid induces selective time dependent decrease in [125I]insulin-like growth factor I, [125I]insulin-like growth factor II and [125I]insulin receptor binding sites in adult rat hippocampal formation.

  1997cites this paper
• Impaired brain development and reduced astrocyte response to injury in transgenic mice expressing IGF binding protein-1.

  1997cites this paper
• Growth factors rescue embryonic dopamine neurons from programmed cell death.

  1996cites this paper
• Expression of insulin‐like growth factor‐1 (IGF‐1) and IGF‐binding protein 2 (IGF‐BP2) in the hippocampus following cytotoxic lesion of the dentate gyrus

  1996cites this paper
• Effects of estradiol on the expression and production of IGFBP-2 by R3230AC mammary tumor cells.

  1996cites this paper
• Distribution and levels of insulin-like growth factor (IGF-I and IGF-II) and insulin receptor binding sites in the spinal cords of amyotrophic lateral sclerosis (ALS) patients.

  1996cites this paper
• Involvement of Na+,K+‐ATPase in the Mitogenic Effect of Insulin‐Like Growth Factor‐I on Cultured Rat Astrocytes

  1996cites this paper
• The early intracellular signaling pathway for the insulin/insulin-like growth factor receptor family in the mammalian central nervous system

  1996cites this paper
• Regulation of the Manganese Superoxide Dismutase and Inducible Nitric Oxide Synthase Gene in Rat Neuronal and Glial Cells

  1996cites this paper
• New aspects of lactate metabolism: IGF-I and insulin regulate mitochondrial function in cultured brain cells during normoxia and hypoxia.

  1996cites this paper
• Insulin-like growth factor receptors and binding proteins.

  1996cites this paper
• Insulin‐Like Growth Factor I Receptor Expression and Function in Nerve Growth Factor‐Differentiated PC12 Cells

  1996cites this paper
• Insulin-like growth factor-1 stimulation of protein synthesis is attenuated in cerebral cortex of aging rats.

  1995cites this paper
• The developing CNS: a scenario for the action of proinsulin, insulin and insulin-like growth factors.

  1995cites this paper
• Effects of growth factors on phosphatidylinositol‐3 kinase in astroglial cells

  1995cites this paper
• Daunomycin inhibits insulin-like growth factor I-dependent protein tyrosine phosphorylation.

  1995cites this paper
• Molecular and cellular aspects of the insulin-like growth factor I receptor.

  1995cites this paper
• Mutation of a Conserved Amino Acid Residue (Tryptophan 1173) in the Tyrosine Kinase Domain of the IGF-I Receptor Abolishes Autophosphorylation but Does Not Eliminate Biologic Function (*)

  1995cites this paper
• Insulin‐like growth factor‐1 mRNA is increased in deafferented hippocampus: Spatiotemporal correspondence of a trophic event with axon sprouting

  1995cites this paper
• Ethanol inhibits insulin receptor substrate-1 tyrosine phosphorylation and insulin-stimulated neuronal thread protein gene expression.

  1995cites this paper
• An immunohistochemical and in situ hybridization study of insulin-like growth factor I within fetal neuron cell cultures.

  1995cites this paper
• Developing oligodendroglia express mRNA for insulin‐like growth factor‐I, a regulator of oligodendrocyte development

  1995cites this paper
• Insulin-like growth factor I increases myelination and inhibits demyelination in cultured organotypic nerve tissue.

  1995cites this paper
• Neuronal insulin receptors in Y79 retinoblastoma cells.

  1995cites this paper
• Effects of ethanol on glucose transporter expression in cultured hippocampal neurons.

  1995cites this paper
• Insulin-like growth factor II promotes in vitro cholinergic development of mouse septal neurons: comparison with the effects of insulin-like growth factor I.

  1994cites this paper
• Effects of insulin and 2-deoxy-D-glucose administration on CNS IGF-II content.

  1994cites this paper
• Synthesis of insulin and its effects in Y79 human retinoblastoma cells.

  1994cites this paper
• Insulin and Insulin‐like Growth Factor System Components Gene Expression in the Chicken Retina From Early Neurogenesis Until Late Development and Their Effect on Neuroepithelial Cells

  1994cites this paper
• Effects of insulin-like growth factors on organotypic cocultures of embryonic rat brainstem slices and skeletal muscle fibers.

  1994cites this paper
• Decreased insulin-like growth factor I-mediated protein tyrosine phosphorylation in human olivopontocerebellar atrophy and lurcher mutant mouse.

  1994cites this paper
• Signaling by insulin-like growth factors in paralyzed skeletal muscle: rapid induction of IGF1 expression in muscle fibers and prevention of interstitial cell proliferation by IGF-BP5 and IGF-BP4

  1994cites this paper
• Expression of insulin-like growth factor-I (IGF-I) receptor gene in rat brain and liver during development and in regenerating adult rat liver.

  1994cites this paper
• Neuropeptide receptors and astrocytes.

  1994cites this paper
• 13 – Insulin-Like Growth Factors in the Brain

  1993cites this paper
• Quantitative autoradiographic localization of [125I]insulin‐like growth factor I, [125I]insulin‐like growth factor II, and [125I]insulin receptor binding sites in developing and adult rat brain

  1993cites this paper
• The developmental pattern of rabbit brain insulin and insulin-like growth factor receptor expression.

  1993cites this paper
• Effects of serum and insulin-like growth factors on human neuroblastoma cell growth.

  1993cites this paper
• Insulin-like growth factor I increases brain growth and central nervous system myelination in transgenic mice.

  1993cites this paper
• 14 - Neurobiology of Insulin and Insulin-Like Growth Factors

  1993cites this paper
• Effect of Growth Factors on Macromolecular Synthesis in Primary Rat Astroglial Cell Cultures a

  1993cites this paper