Methods for generating precise deletions and insertions in the genome of wild-type Escherichia coli: application to open reading frame characterization

No abstract is available for this paper.

• Publication year

  1997

• Venue

  Journal of Bacteriology

• Publication date

  1997-10-01

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1128/jb.179.20.6228-6237.1997PMID 9335267PMCID PMC179534
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K‐12

  1997cited by this paper
• MurA (MurZ), the enzyme that catalyzes the first committed step in peptidoglycan biosynthesis, is essential in Escherichia coli

  1995cited by this paper
• Physical map location of a set of Escherichia coli genes (hde) whose expression is affected by the nucleoid protein H-NS

  1993cited by this paper
• Function of the Escherichia coli nucleoid protein, H-NS molecular analysis of a subset of proteins whose expression is enhanced in a hns deletion mutant

  1993cited by this paper
• Simple phagemid-based system for generating allele replacements in Escherichia coli

  1993cited by this paper
• Multiplex DNA sequencing.

  1993influential reference
• Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium

  1993cited by this paper
• PCR amplification of long DNA fragments.

  1992cited by this paper
• Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector.

  1992cited by this paper
• Mapping sequenced E.coli genes by computer: software, strategies and examples.

  1991cited by this paper
• Allelic exchange in Escherichia coli using the Bacillus subtilis sacB gene and a temperature‐sensitive pSC101 replicon

  1991cited by this paper
• Uses of transposons with emphasis on Tn10.

  1991cited by this paper
• Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.

  1991cited by this paper
• Introduction of a subtle mutation into the Hox-2.6 locus in embryonic stem cells

  1991cited by this paper
• Introduction of a subtle mutation into the Hox-2.6 locus in embryonic stem cells

  1991cited by this paper
• Bacteriophage P1 cloning system for the isolation, amplification, and recovery of DNA fragments as large as 100 kilobase pairs.

  1990cited by this paper
• Improved double-stranded DNA sequencing using the linear polymerase chain reaction.

  1989cited by this paper
• Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extension.

  1989cited by this paper
• Methionine aminopeptidase gene of Escherichia coli is essential for cell growth

  1989cited by this paper
• Site-directed mutagenesis by overlap extension using the polymerase chain reaction.

  1989cited by this paper
• New method for generating deletions and gene replacements in Escherichia coli

  1989influential reference
• Effect of base pair mismatches on recombination via the RecBCD pathway

  1989cited by this paper
• Homologous Recombination in Procaryotes

  1988cited by this paper
• Multiplex DNA sequencing.

  1988cited by this paper
• Transposition of IS50L activates downstream genes

  1988cited by this paper
• Transcription attenuation.

  1988cited by this paper
• Activation of silent genes by transposons Tn5 and Tn10.

  1988cited by this paper
• Construction of an Escherichia coli K-12 ada deletion by gene replacement in a recD strain reveals a second methyltransferase that repairs alkylated DNA

  1988cited by this paper
• Mechanism and control of homologous recombination in Escherichia coli.

  1987cited by this paper
• 5-Fluoroorotic acid as a selective agent in yeast molecular genetics.

  1987cited by this paper
• Homologous recombination in Escherichia coli: dependence on substrate length and homology.

  1986cited by this paper
• Directionality in FLP protein-promoted site-specific recombination is mediated by DNA-DNA pairing.

  1986cited by this paper
• Positive selection procedure for entrapment of insertion sequence elements in gram-negative bacteria

  1985cited by this paper
• Site-directed insertion and deletion mutagenesis with cloned fragments in Escherichia coli

  1985cited by this paper
• Deletion of an essential gene in Escherichia coli by site-specific recombination with linear DNA fragments

  1984cited by this paper
• In vitro insertional mutagenesis with a selectable DNA fragment.

  1984cited by this paper
• Replacement and amplification of bacterial genes with sequences altered in vitro.

  1983cited by this paper
• On the formation of spontaneous deletions: the importance of short sequence homologies in the generation of large deletions.

  1982cited by this paper
• Transposon Tn10 provides a promoter for transcription of adjacent sequences.

  1982cited by this paper
• Protein localization in E. coli: is there a common step in the secretion of periplasmic and outer-membrane proteins?

  1981cited by this paper
• A rapid alkaline extraction procedure for screening recombinant plasmid DNA.

  1979cited by this paper
• Replacement of chromosome segments with altered DNA sequences constructed in vitro.

  1979cited by this paper
• Use of gene fusion to study secretion of maltose-binding protein into Escherichia coli periplasm

  1979cited by this paper
• A conditionally lethal mutation of Escherichia coli affecting the gene coding for ribosomal protein S2 (rpsB).

  1979cited by this paper
• Levansucrase of Bacillus subtilis

  1976cited by this paper
• [86] Levansucrase from Bacillus subtilis

  1966cited by this paper

• Physics of swimming and its fitness cost determine strategies of bacterial investment in flagellar motility

  2025cites this paper
• An Hfq-dependent post-transcriptional mechanism fine tunes RecB expression in Escherichia coli

  2025cites this paper
• Colistin resistance plasmids dually enhance bacterial virulence and antibiotic resistance via surface polysaccharide biosynthesis

  2025cites this paper
• Biofilm architecture determines the dissemination of conjugative plasmids

  2025cites this paper
• ArcA modulates multidrug resistance and compound susceptibility in Klebsiella pneumoniae through ArcB-independent regulation of the SMR efflux pump kpnEF

  2025cites this paper
• Role of GuaB, the inosine-5′-monophosphate dehydrogenase of uropathogenic Escherichia coli pathogenicity: a key factor for bladder infection

  2025cites this paper
• RecN and RecA orchestrate an ordered DNA supercompaction response following ciprofloxacin-induced DNA damage in Escherichia coli

  2025cites this paper
• Characterization of a Phage-Encoded Depolymerase Against Klebsiella pneumoniae K30 Capsular Type and Its Therapeutic Application in a Murine Model of Aspiration Pneumonia

  2025cites this paper
• Efficient bioproduction of poly(3-hydroxypropionate) homopolymer using engineered Escherichia coli strains.

  2024cites this paper
• Selective bacteriophages reduce the emergence of resistant bacteria in bacteriophage-antibiotic combination therapy

  2024cites this paper
• TusDCB, a sulfur transferase complex involved in tRNA modification, contributes to UPEC pathogenicity

  2024cites this paper
• An improved temperature-sensitive shuttle vector system for scarless gene deletion in human-gut-associated Bifidobacterium species

  2024cites this paper
• Non-canonical start codons confer context-dependent advantages in carbohydrate utilization for commensal E. coli in the murine gut

  2024cites this paper
• Physics and physiology determine strategies of bacterial investment in flagellar motility

  2024cites this paper
• Advancing vaccine technology through the manipulation of pathogenic and commensal bacteria

  2024cites this paper
• Purification and catalysis of choline dehydrogenase from Escherichia coli.

  2024cites this paper
• Conformational ensembles in Klebsiella pneumoniae FimH impact uropathogenesis

  2024cites this paper
• Substrate geometry affects population dynamics in a bacterial biofilm

  2023cites this paper
• Glucose Induces Resistance to Polymyxins in High-Alcohol-Producing Klebsiella pneumoniae via Increasing Capsular Polysaccharide and Maintaining Intracellular ATP

  2023cites this paper
• Inactivation of ackA and pta Genes Reduces GlpT Expression and Susceptibility to Fosfomycin in Escherichia coli

  2023cites this paper
• Targeted Mutagenesis of Mycobacterium Strains by Homologous Recombination.

  2023cites this paper
• Role of the Escherichia coli ubiquinone-synthesizing UbiUVT pathway in adaptation to changing respiratory conditions

  2023influential citation
• The role of integration host factor in biofilm and virulence of high-alcohol-producing Klebsiella pneumoniae

  2023cites this paper
• Evolution of a cross-feeding interaction following a key innovation in a long-term evolution experiment with Escherichia coli

  2023cites this paper
• Selective bacteriophages reduce the emergence of resistant bacteria in the bacteriophage-antibiotic combination therapy

  2023cites this paper
• Synthesis of neuraminidase-resistant sialyllactose mimetics from Nacyl mannosamines using metabolically engineered Escherichia coli.

  2023cites this paper
• No fitness cost entailed by type VI secretion system synthesis, assembly, contraction, or disassembly in enteroaggregative Escherichia coli

  2023cites this paper
• The PapB/FocB family protein TosR acts as a positive regulator of flagellar expression and is required for optimal virulence of uropathogenic Escherichia coli

  2023cites this paper
• Achieving high titer and yield in the bioconversion of l-threonine to 2-hydroxybutyric acid with Escherichia coli BL21

  2023cites this paper
• A Simple Allelic Exchange Method for Efficient Seamless Knockout of Up to 34-kbp-Long Gene Cassettes in Pseudomonas

  2023cites this paper
• Safety evaluation of the food enzyme d‐tagatose 3‐epimerase from the genetically modified Escherichia coli strain PS‐Sav‐001

  2023cites this paper
• Molecular mechanism of plasmid-borne resistance to sulfonamide antibiotics

  2023cites this paper
• Metabolic engineering of Escherichia coli for biological production of 1, 3-Butanediol.

  2023cites this paper
• Role of the Escherichia coli ubiquinone-synthesizing UbiUVT pathway in adaptation to changing respiratory conditions

  2023cites this paper
• The Oligosaccharide Region of LPS Governs Predation of E. coli by the Bacterivorous Protist, Acanthamoeba castellanii

  2023cites this paper
• Versatile allelic replacement and self-excising integrative vectors for plasmid genome mutation and complementation

  2023cites this paper
• Biotechnological production of sialylated solid lipid microparticles as inhibitors of influenza A virus infection.

  2022cites this paper
• A universal coupling mechanism of respiratory complex I

  2022cites this paper
• Hijacking the Peptidoglycan Recycling Pathway of Escherichia coli to Produce Muropeptides

  2022cites this paper
• Identification of the BolA Protein Reveals a Novel Virulence Factor in K. pneumoniae That Contributes to Survival in Host

  2022cites this paper
• Metabolic engineering of Escherichia coli W3110 for efficient production of homoserine from glucose.

  2022cites this paper
• Deletion of proU suppresses proQ phenotypes in Escherichia coli

  2022cites this paper
• Identification of a two-component regulatory system involved in antimicrobial peptide resistance in Streptococcus pneumoniae

  2022influential citation
• Structural and mechanistic analysis of a tripartite ATP-independent periplasmic TRAP transporter

  2022cites this paper
• Metabolic and Morphotypic Trade-Offs within the Eco-Evolutionary Dynamics of Escherichia coli

  2022cites this paper
• Molecular mechanism of plasmid-borne resistance to sulfonamides

  2022cites this paper
• Transporter Genes and fosA Associated With Fosfomycin Resistance in Carbapenem-Resistant Klebsiella pneumoniae

  2022cites this paper
• Development of efficient 5-ketogluconate production system by Gluconobacter japonicus

  2022cites this paper
• Stochastic chain termination in bacterial pilus assembly

  2022cites this paper
• Coevolution-Guided Mapping of the Type VI Secretion Membrane Complex-Baseplate Interface.

  2022cites this paper
• Genetic Circuit Design in Rhizobacteria

  2022cites this paper
• Improvement of 3-Hydroxypropionic Acid Tolerance in Klebsiella pneumoniae by Novel Transporter YohJK.

  2021cites this paper
• The structure of HiSiaQM defines the architecture of tripartite ATP-independent periplasmic (TRAP) transporters

  2021cites this paper
• Distinct heterochromatin‐like domains promote transcriptional memory and silence parasitic genetic elements in bacteria

  2021cites this paper
• Inhibiting Type VI Secretion System Activity with a Biomimetic Peptide Designed To Target the Baseplate Wedge Complex

  2021cites this paper
• Development of efficient microbial cell factory for whole-cell bioconversion of L-threonine to 2-hydroxybutyric acid.

  2021cites this paper
• Repurposing CRISPR-Cas Systems as Genetic Tools for the Enterobacteriales

  2021cites this paper
• Plasmid replication based on the T7 origin of replication requires a T7 RNAP variant and inactivation of ribonuclease H

  2021cites this paper
• Dual Regulation of Phosphatidylserine Decarboxylase Expression by Envelope Stress Responses

  2021cites this paper
• Whither Bioinformatics?

  2021cites this paper
• A Tryptophan ‘Gate’ in the CRISPR-Cas3 Nuclease Controls ssDNA Entry into the Nuclease Site, That When Removed Results in Nuclease Hyperactivity

  2021cites this paper
• CRISPR/Cas12a‐mediated genome engineering in the photosynthetic bacterium Rhodobacter capsulatus

  2021cites this paper
• Production and Characterization of Motile and Chemotactic Bacterial Minicells

  2021cites this paper
• Roles of OmpX, an Outer Membrane Protein, on Virulence and Flagellar Expression in Uropathogenic Escherichia coli

  2021cites this paper
• Botulinum Toxin: An Update on Pharmacology and Newer Products in Development

  2021cites this paper
• Genetic determinants facilitating the evolution of resistance to carbapenem antibiotics

  2021cites this paper
• Biosensor-Based Multigene Pathway Optimization for Enhancing the Production of Glycolate

  2021cites this paper
• Roles of OmpA in Type III Secretion System-Mediated Virulence of Enterohemorrhagic Escherichia coli

  2021influential citation
• Fine-tuning of RecBCD expression by post-transcriptional regulation is required for optimal DNA repair in Escherichia coli

  2021cites this paper
• Production of perdeuterated fucose from glyco-engineered bacteria

  2020cites this paper
• Participation of two general stress response proteins from Xanthomonas citri subsp. citri in environmental stress adaptation and virulence.

  2020cites this paper
• Efficient long fragment editing technique enables large-scale and scarless bacterial genome engineering

  2020cites this paper
• Efficient dual-negative selection for bacterial genome editing

  2020cites this paper
• RskA Is a Dual Function Activator-Inhibitor That Controls SigK Activity Across Distinct Bacterial Genera

  2020cites this paper
• The Type IX Secretion System Is Required for Virulence of the Fish Pathogen Flavobacterium psychrophilum

  2020cites this paper
• Development of Klebsiella pneumoniae J2B as microbial cell factory for the production of 1,3-propanediol from glucose.

  2020cites this paper
• Genome-scale metabolic rewiring to achieve predictable titers rates and yield of a non-native product at scale

  2020cites this paper
• Extreme Antibiotic Persistence via Heterogeneity-Generating Mutations Targeting Translation

  2020cites this paper
• Efficient long fragment editing technique enables rapid construction of genetically stable bacterial strains

  2020cites this paper
• Development of a Sensitive Escherichia coli Bioreporter Without Antibiotic Markers for Detecting Bioavailable Copper in Water Environments

  2020cites this paper
• Roles of CytR, an anti-activator of cyclic-AMP receptor protein (CRP) on flagellar expression and virulence in uropathogenic Escherichia coli.

  2020cites this paper
• Identification of Genetic Variants via Bacterial Respiration Gas Analysis

  2020cites this paper
• Donor plasmids for phenotypically neutral chromosomal gene insertions in Enterobacteriaceae.

  2020cites this paper
• Production of 3-hydroxypropionic acid from acetate using metabolically-engineered and glucose-grown Escherichia coli.

  2020cites this paper
• Genome-scale metabolic rewiring improves titers rates and yields of the non-native product indigoidine at scale

  2020cites this paper
• Systems evaluation reveals novel transporter YohJK renders 3-hydroxypropionate tolerance in Escherichia coli

  2020cites this paper
• Diet posttranslationally modifies the mouse gut microbial proteome to modulate renal function

  2020cites this paper
• The immunity of Meiwa kumquat against Xanthomonas citri is associated with a known susceptibility gene induced by a transcription activator-like effector

  2020cites this paper
• Diet Post-translationally Modifies The Gut Microbial Proteome To Modulate Renal Function

  2020cites this paper
• Bacterial Genetic Engineering by Means of Recombineering for Reverse Genetics

  2020cites this paper
• Roles of the Tol-Pal system in the Type III secretion system and flagella-mediated virulence in enterohemorrhagic Escherichia coli

  2020cites this paper
• Production of bio-xylitol from d-xylose by an engineered Pichia pastoris expressing a recombinant xylose reductase did not require any auxiliary substrate as electron donor

  2020cites this paper
• Mutations in domain IV of elongation factor EF-G confer −1 frameshifting

  2020cites this paper
• Bacterial chromosome replication: does precatenation occur in vivo?

  2019cites this paper
• Single cell measurements of bacterial physiology traits during exposure to an external stress

  2019influential citation
• Regulation of antimonite oxidation and resistance by the phosphate regulator PhoB in Agrobacterium tumefaciens GW4.

  2019cites this paper
• Development of 3-hydroxypropionic-acid-tolerant strain of Escherichia coli W and role of minor global regulator yieP.

  2019cites this paper
• Plasticity of Promoter-Core Sequences Allows Bacteria to Compensate for the Loss of a Key Global Regulatory Gene.

  2019cites this paper
• In situ and high‐resolution cryo‐EM structure of a bacterial type VI secretion system membrane complex

  2019cites this paper
• Comparative Analysis of Ionic Strength Tolerance between Freshwater and Marine Caulobacterales Adhesins

  2019cites this paper