Purification and properties of the sigma subunit of Escherichia coli DNA-dependent RNA polymerase.

No abstract is available for this paper.

• Publication year

  1979

• Venue

  Biochemistry

• Publication date

  1979-04-03

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1021/BI00574A034PMID 371677
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Mutation affecting thermostability of sigma subunit of Escherichia coli RNA polymerase lies near the dnaG locus at about 66 min on the E. coli genetic map.

  1978cited by this paper
• A mutation affecting the σ subunit of RNA polymerase changes transcriptional specificity

  1978cited by this paper
• Temperature-sensitive Escherichia coli mutant producing a temperature-sensitive sigma subunit of DNA-dependent RNA polymerase.

  1978cited by this paper
• Nonspecific interactions of Escherichia coli RNA polymerase with native and denatured DNA: differences in the binding behavior of core and holoenzyme.

  1978cited by this paper
• A mutation affecting the sigma subunit of RNA polymerase changes transcriptional specificity.

  1978cited by this paper
• RNA polymerase mutant with altered sigma factor in Escherichia coli

  1978cited by this paper
• Over-synthesis and instability of sigma protein in a merodiploid strain of Escherichia coli

  1978cited by this paper
• Primary structure of alpha-subunit of DNA-dependent RNA polymerase from Escherichia coli.

  1977cited by this paper
• Chromosomal location of a structural gene for the RNA polymerase sigma factor in Escherichia coli.

  1977cited by this paper
• Primary structure of α‐subunit of DNA‐dependent RNA polymerase from Escherichia coli

  1977cited by this paper
• Isolation of amino-terminal fragment of lactose repressor necessary for DNA binding.

  1977cited by this paper
• Complete amino acid sequence of rabbit muscle glycogen phosphorylase.

  1977cited by this paper
• Templates for eukaryotic RNA polymerase II: artefacts can produce an apparent preference for denatured DNA over native DNA.

  1977cited by this paper
• The amino acid sequence of beta-galactosidase of Escherichia coli.

  1977cited by this paper
• A gene from Escherichia coli affecting the sigma subunit of RNA polymerase.

  1977cited by this paper
• Structural properties of Escherichia coli RNA polymerase Subunits.

  1976cited by this paper
• A transducing bacteriophage λ carrying the structural gene for elongation factor Ts

  1976cited by this paper
• Cysteine and methionine content of the Escherichia coli ribonucleic acid polymerase subunits

  1976cited by this paper
• Rapid micromethod for the purification of Escherichia coli ribonucleic acid polymerase and the preparation of bacterial extracts active in ribonucleic acid synthesis

  1976cited by this paper
• 3 – Proteins and Sodium Dodecyl Sulfate: Molecular Weight Determination on Polyacrylamide Gels and Related Procedures

  1975cited by this paper
• High resolution two-dimensional electrophoresis of proteins.

  1975cited by this paper
• Isolation and characterization of an alpha-amanitin-resistant rat myoblast mutant cell line possessing alpha-amanitin-resistant RNA polymerase II.

  1975cited by this paper
• The subunits of DNA‐dependent RNA polymerase from E. coli: I. Amino acid analysis and primary structure of the N‐terminal regions

  1975cited by this paper
• Discrepancy in the mobility of a protein of phage phi29 in 2 different SDS polyacrylamide-gel systems.

  1975cited by this paper
• A procedure for the rapid, large-scall purification of Escherichia coli DNA-dependent RNA polymerase involving Polymin P precipitation and DNA-cellulose chromatography.

  1975cited by this paper
• RNA polymerase assembly in vitro. Temperature dependence of reactivation of denatured core enzyme.

  1974cited by this paper
• Measurement of protein by spectrophotometry at 205 nm.

  1974cited by this paper
• Sodium dodecylsulfate-dependent anomalies in gel electrophoresis: alterations in the banding patterns of foot-and-mouth disease virus polypeptides.

  1974cited by this paper
• A rapid, sensitive, and specific method for the determination of protein in dilute solution.

  1973cited by this paper
• Chromatography of rNA polymerase from escherichia coli on single stranded DNA-agarose columns.

  1972cited by this paper
• Affinity chromatography of DNA-binding enzymes on single-stranded DNA-agarose columns.

  1972cited by this paper
• Quantitative procedures for use with the Edman-Begg sequenator. Partial sequences of two unusual immunoglobulin light chains, Rzf and Sac.

  1971cited by this paper
• [43] Purification of two forms of Escherichia coli RNA polymerase and of sigma component

  1971cited by this paper
• RNA polymerase.

  1971cited by this paper
• [42] Purification of the RNA polymerase sigma factor☆

  1971cited by this paper
• On the Rule of Different Subunits of DNA-Dependent RNA Polymerase from E. coli in the Transcription Process

  1970cited by this paper
• Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

  1970cited by this paper
• Cyclic Re-use of the RNA Polymerase Sigma Factor

  1969cited by this paper
• Practical spectrophotometric standards.

  1969cited by this paper
• Factor Stimulating Transcription by RNA Polymerase

  1969cited by this paper
• Separation and characterization of the subunits of ribonucleic acid polymerase.

  1969cited by this paper
• Examination of the dissociation of multichain proteins in guanidine hydrochloride by membrane osmometry.

  1968cited by this paper
• Proteins as random coils. 3. Optical rotatory dispersion in 6 M guanidine hydrochloride.

  1967cited by this paper
• Spectroscopic determination of tryptophan and tyrosine in proteins.

  1967cited by this paper
• On the determination of cystine as cysteic acid.

  1963cited by this paper

• A saponin-polybromophenol antibiotic (CU1) from Cassia fistula Bark Against Multi-Drug Resistant Bacteria Targeting RNA polymerase

  2022cites this paper
• What is in the black box? The discovery of the sigma factor and the subunit structure of E. coli RNA polymerase

  2021cites this paper
• An Abundant New Saponin-Polybromophenol Antibiotic (CU1) from Cassia fistula Bark Against Multi-Drug Resistant Bacteria Targeting RNA Polymerase

  2020cites this paper
• Growth phase-specific changes in the composition of E. coli transcription complexes.

  2019cites this paper
• Coupling flagellar gene expression to flagellar assembly in Caulobacter crescentus

  2012cites this paper
• Co-evolution of RNA polymerase with RbpA in the phylum Actinobacteria

  2012cites this paper
• Effects of substitutions at position 180 in the Escherichia coli RNA polymerase σ70 subunit

  2011cites this paper
• ROMA: an in vitro approach to defining target genes for transcription regulators.

  2009cites this paper
• Energetic contributions to the initiation of transcription in E. coli.

  2008cites this paper
• Isolation of DNA-dependent RNA polymerase fromStreptomyces granaticolor and its binding to phage ϕ29 DNA

  2008cites this paper
• Molecular basis for regulation of the heat shock transcription factor sigma32 by the DnaK and DnaJ chaperones.

  2008cites this paper
• Identification and Characterization of the dps Promoter of Mycobacterium smegmatis: Promoter Recognition by Stress-Specific Extracytoplasmic Function Sigma Factors σH and σF

  2007cites this paper
• Identification and characterization of the dps promoter of Mycobacterium smegmatis: promoter recognition by stress-specific extracytoplasmic function sigma factors sigmaH and sigmaF.

  2007cites this paper
• The evolving story of the omega subunit of bacterial RNA polymerase.

  2006cites this paper
• Isolation of RNA Polymerase from Clostridium difficile and Characterization of Glutamate Dehydrogenase and rRNA Gene Promoters In Vitro and In Vivo

  2006cites this paper
• Regulation of Bacterial Transcription by Anti-σ Factors

  2005cites this paper
• Altering the interaction between sigma70 and RNA polymerase generates complexes with distinct transcription-elongation properties.

  2005cites this paper
• Transcription of T7 DNA immobilised on latex beads and Langmuir-Blodgett film.

  2005cites this paper
• Deletion of the gene rpoZ, encoding the omega subunit of RNA polymerase, in Mycobacterium smegmatis results in fragmentation of the beta' subunit in the enzyme assembly.

  2005cites this paper
• Deletion of the Gene rpoZ, Encoding the ω Subunit of RNA Polymerase, in Mycobacterium smegmatis Results in Fragmentation of the β′ Subunit in the Enzyme Assembly

  2005cites this paper
• Genetic studies on the β subunit of Escherichia coli RNA polymerase

  2004cites this paper
• Specific stimulation of ribosomal RNA synthesis in E. coli by a protein factor

  2004cites this paper
• lacP1 Promoter with an Extended – 10 Motif

  2004cites this paper
• Nucleotide sequence of a promoter recognized by Bacillus subtilis RNA polymerase

  2004cites this paper
• Intragenic localization of amber and temperature-sensitive rpoD mutations affecting RNA polymerase sigma factor of Escherichia coli

  2004cites this paper
• A new mutation rpoD800, affecting the sigma subunit of E. coli RNA polymerase is allelic to two other sigma mutants

  2004cites this paper
• Genetic studies on the β subunit of Escherichia coli RNA polymerase

  2004cites this paper
• Entropic nature of the interaction between promoter bound CRP mutants and RNA polymerase.

  2003cites this paper
• Purification of Bacillus subtilis RNA polymerase and associated factors.

  2003cites this paper
• Inter-subunit recognition and manifestation of segmental mobility in Escherichia coli RNA polymerase: a case study with ω–β′ interaction

  2003cites this paper
• Multiple sigma subunits and the partitioning of bacterial transcription space.

  2003cites this paper
• Purification of highly-active and soluble Escherichia coli sigma 70 polypeptide overproduced at low temperature.

  2003cites this paper
• Kinetics of Transcription Initiation at lacP1

  2003cites this paper
• Inter-subunit recognition and manifestation of segmental mobility in Escherichia coli RNA polymerase: a case study with omega-beta' interaction.

  2003cites this paper
• The RNA Polymerase α Subunit from Sinorhizobium meliloti Can Assemble with RNA Polymerase Subunits from Escherichia coli and Function in Basal and Activated Transcription both In Vivo and In Vitro

  2002cites this paper
• The RNA polymerase alpha subunit from Sinorhizobium meliloti can assemble with RNA polymerase subunits from Escherichia coli and function in basal and activated transcription both in vivo and in vitro.

  2002cites this paper
• In Vitro Repression of the gal Promoters by GalR and HU Depends on the Proper Helical Phasing of the Two Operators*

  2002cites this paper
• FIS modulates the kinetics of successive interactions of RNA polymerase with the core and upstream regions of the tyrT promoter.

  2002cites this paper
• The RNA Polymerase (cid:2) Subunit from Sinorhizobium meliloti Can Assemble with RNA Polymerase Subunits from Escherichia coli and Function in Basal and Activated Transcription both In Vivo and In Vitro

  2002cites this paper
• Topography of lacUV5 initiation complexes.

  2001cites this paper
• Mutations at position -10 in the lambda PR promoter primarily affect conversion of the initial closed complex (RPc) to a stable, closed intermediate (RPi).

  2001cites this paper
• Escherichia coli RNA polymerase subunit ω and its N‐terminal domain bind full‐length β′ to facilitate incorporation into the α2β subassembly

  2001cites this paper
• Escherichia coli RNA polymerase subunit v and its N-terminal domain bind full-length b 0 to facilitate incorporation into the a 2 b subassembly

  2001cites this paper
• Escherichia coli RNA polymerase subunit omega and its N-terminal domain bind full-length beta' to facilitate incorporation into the alpha2beta subassembly.

  2001cites this paper
• Binding of the Transcription Effector ppGpp to Escherichia coli RNA Polymerase Is Allosteric, Modular, and Occurs Near the N Terminus of the β′-Subunit*

  2001cites this paper
• Amino acid substitution at position 99 affects the rate of CRP subunit exchange.

  2001cites this paper
• The C Terminus of ς32 Is Not Essential for Degradation by FtsH

  2001cites this paper
• Coactivation of the RpoS-Dependent proPP2 Promoter by Fis and Cyclic AMP Receptor Protein

  2000cites this paper
• RNA-protein crosslinking to AMP residues at internal positions in RNA with a new photocrosslinking ATP analog.

  2000cites this paper
• Interaction of sigma factor sigmaN with Escherichia coli RNA polymerase core enzyme.

  2000cites this paper
• Changes in the 17 bp spacer in the P(R) promoter of bacteriophage lambda affect steps in open complex formation that precede DNA strand separation.

  2000cites this paper
• Interaction between RNA Polymerase and RapA, a Bacterial Homolog of the SWI/SNF Protein Family*

  2000cites this paper
• RNA Polymerase-cNMP-ligated cAMP Receptor Protein (CRP) Mutant Interactions in the Enhancement of Transcription by CRP Mutants*

  2000cites this paper
• Interaction of σ70 with Escherichia coli RNA polymerase core enzyme studied by surface plasmon resonance

  2000cites this paper
• Interaction of sigma 70 with Escherichia coli RNA polymerase core enzyme studied by surface plasmon resonance.

  2000cites this paper
• Mapping protease susceptibility sites on the Escherichia coli transcription factor sigma70.

  1999cites this paper
• Localization of amino acids required for Fis to function as a class II transcriptional activator at the RpoS-dependent proP P2 promoter.

  1999cites this paper
• A RNA polymerase with transcriptional activity at 0°C from the Antarctic bacterium Pseudomonas syringae

  1999cites this paper
• The kinetics of sigma subunit directed promoter recognition by E. coli RNA polymerase.

  1999cites this paper
• Real time in vitro analysis of transcription by RNA polymerase on immobilized DNA fibres

  1999cites this paper
• Core RNA polymerase from E. coli induces a major change in the domain arrangement of the sigma 70 subunit.

  1999influential citation
• A RNA polymerase with transcriptional activity at 0 degrees C from the Antarctic bacterium Pseudomonas syringae.

  1999cites this paper
• A mutation in region 1.1 of σ70 affects promoter DNA binding by Escherichia coli RNA polymerase holoenzyme

  1999cites this paper
• Role of Region C in Regulation of the Heat Shock Gene-Specific Sigma Factor of Escherichia coli, ς32

  1999cites this paper
• Transcriptional Activity at Supraoptimal Temperature of Growth in the Antarctic Psychrotrophic Bacterium Pseudomonas syringae

  1999cites this paper
• Position 127 amino acid substitutions affect the formation of CRP:cAMP:lacP complexes but not CRP:cAMP:RNA polymerase complexes at lacP.

  1999cites this paper
• Promoter upstream bent DNA activates the transcription of the Clostridium perfringens phospholipase C gene in a low temperature‐dependent manner

  1999cites this paper
• GroEL is involved in activation of Escherichia coli RNA polymerase devoid of the omega subunit in vivo.

  1999cites this paper
• Conformational Changes of Escherichia coli RNA Polymerase ς70 Factor Induced by Binding to the Core Enzyme*

  1998cites this paper
• Guanosine tetraphosphate-induced dissociation of open complexes at the Escherichia coli ribosomal protein promoters rplJ and rpsA P1: nanosecond depolarization spectroscopic studies.

  1998cites this paper
• Promoter selectivity of the Bacillus subtilis RNA polymerase sigmaA and sigmaH holoenzymes.

  1998cites this paper
• Stimulation of Open Complex Formation by Nicks and Apurinic Sites Suggests a Role for Nucleation of DNA Melting in Escherichia coli Promoter Function*

  1998cites this paper
• The flagellar anti-sigma factor FlgM actively dissociates Salmonella typhimurium sigma28 RNA polymerase holoenzyme.

  1998cites this paper
• RapA, a Novel RNA Polymerase-Associated Protein, Is a Bacterial Homolog of SWI2/SNF2*

  1998cites this paper
• Levels of DnaK and DnaJ provide tight control of heat shock gene expression and protein repair in Escherichia coli

  1998cites this paper
• Characterization of the Closed Complex Intermediate Formed during Transcription Initiation by Escherichia coli RNA Polymerase*

  1998cites this paper
• Conformation and DNA binding properties of a single-stranded DNA binding region of sigma 70 subunit from Escherichia coli RNA polymerase are modulated by an interaction with the core enzyme.

  1998cites this paper
• Interaction of Escherichia coli sigma 70 with core RNA polymerase.

  1998cites this paper
• Studies on the omega subunit of Escherichia coli RNA polymerase--its role in the recovery of denatured enzyme activity.

  1997cites this paper
• In vitro transcription of Myxococcus xanthus genes with RNA polymerase containing σA, the major sigma factor in growing cells

  1997cites this paper
• Changing the mechanism of transcriptional activation by phage λ repressor

  1997cites this paper
• Activation of RpoS-dependent proP P2 transcription by the Fis protein in vitro.

  1997cites this paper
• Mutations in the 1.1 subdomain of Escherichia coli sigma factor sigma70 and disruption of its overall structure.

  1997cites this paper
• Regulation of the elongation-termination decision at intrinsic terminators by antitermination protein N of phage lambda.

  1997cites this paper
• Specificity of an Escherichia coli RNA polymerase-associated NTPase.

  1997cites this paper
• Pribnow Box Recognition and Melting by Escherichia coli RNA Polymerase

  1997cites this paper
• A sigma32 mutant with a single amino acid change in the highly conserved region 2.2 exhibits reduced core RNA polymerase affinity.

  1997cites this paper
• Comparative analysis of the interactions of Escherichia coli sigma S and sigma 70 RNA polymerase holoenzyme with the stationary-phase-specific bolAp1 promoter.

  1997cites this paper
• Changing the mechanism of transcriptional activation by phage lambda repressor.

  1997cites this paper
• Amino Acid Substitutions in the Two Largest Subunits of Escherichia coli RNA Polymerase That Suppress a Defective Rho Termination Factor Affect Different Parts of the Transcription Complex*

  1996cites this paper
• Domain organization of the Escherichia coli RNA polymerase sigma 70 subunit.

  1996cites this paper
• Identification of epitope recognized by an anti-c-myc monoclonal antibody that cross-reacts with E. coli sigma factor using phage display libraries.

  1996cites this paper
• Binding of the sigma 70 protein to the core subunits of Escherichia coli RNA polymerase, studied by iron-EDTA protein footprinting.

  1996cites this paper
• Rickettsia prowazekii sigma factor sigma 73 can be overexpressed in Escherichia coli and promotes RNA polymerase binding and transcription.

  1996cites this paper
• Control of AlgU, a member of the sigma E-like family of stress sigma factors, by the negative regulators MucA and MucB and Pseudomonas aeruginosa conversion to mucoidy in cystic fibrosis

  1996cites this paper
• A cycle of binding and release of the DnaK, DnaJ and GrpE chaperones regulates activity of the Escherichia coli heat shock transcription factor sigma32.

  1996cites this paper
• Characterization of RNA polymerse and two sigma‐factor genes from Mycobacterium smegmatis

  1995cites this paper
• In vitro transcription of pathogenesis-related genes by purified RNA polymerase from Staphylococcus aureus

  1995cites this paper
• Specificity of DNA binding activity of the Bacillus subtilis catabolite control protein CcpA

  1995cites this paper
• Isolation and characterization of inhibitory factors of DNA polymerase III holoenzyme from Escherichia coli.

  1995cites this paper