Calpain Cleavage of the Cytoplasmic Domain of the Integrin β2 Subunit (*)

The cytoplasmic domains of integrin β subunits are involved in bidirectional transmembrane signaling. We report that the cytoplasmic domain of the integrin β3 subunit undergoes limited proteolysis by calpain, an intracellular calcium-dependent protease. Calpain cleavage occurs during platelet aggregation induced by agonists such as thrombin. Five cleavage sites have been identified. Four of these sites (C-terminal to Thr741, Tyr747, Phe754, and Tyr759) are utilized in intact platelets and flank two NXXY motifs (Asn744-Pro-Leu-Tyr747 and Asn756-Ile-Thr-Tyr759). The fifth site (Ala735) is accessible to calpain after EDTA treatment of the αIIbβ3 heterodimer. The NXXY motif is critical to the bidirectional signaling functions of β3 integrins and their association with the cytoskeleton. Thus, calpain cleavage of the β3 cytoplasmic domain may provide a means to regulate integrin signaling functions.

• Publication year

  1995

• Venue

  Journal of Biological Chemistry

• Publication date

  1995-11-03

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1074/jbc.270.44.26146PMID 7592818
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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