Simultaneous Recovery of RNA and DNA from Soils and Sediments

No abstract is available for this paper.

• Publication year

  2001

• Venue

  Applied and Environmental Microbiology

• Publication date

  2001-10-01

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1128/AEM.67.10.4495-4503.2001PMID 11571148PMCID PMC93195
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Particle size analysis

  2013cited by this paper
• A versatile oxygenator and perfusion system for magnetic resonance studies.

  2010cited by this paper
• Molecular Cloning: A Laboratory Manual

  2001cited by this paper
• Nitrite Reductase Genes (nirK andnirS) as Functional Markers To Investigate Diversity of Denitrifying Bacteria in Pacific Northwest Marine Sediment Communities

  2000cited by this paper
• Critical factors influencing the recovery and integrity of rRNA extracted from environmental samples: use of an optimized protocol to measure depth-related biomass distribution in freshwater sediments.

  2000cited by this paper
• Quantification of Bias Related to the Extraction of DNA Directly from Soils

  1999cited by this paper
• Quantitation of the Population Size and Metabolic Activity of a Resin Acid Degrading Bacterium in Activated Sludge Using Slot-Blot Hybridization to Measure the rRNA:rDNA Ratio

  1999cited by this paper
• Killing two birds with one stone: simultaneous extraction of DNA and RNA from activated sludge biomass

  1999cited by this paper
• mRNA degradation in bacteria.

  1999cited by this paper
• Nucleic Acid (DNA, RNA) Quantification and RNA/DNA Ratio Determination in Marine Sediments: Comparison of Spectrophotometric, Fluorometric, and HighPerformance Liquid Chromatography Methods and Estimation of Detrital DNA

  1998cited by this paper
• Extraction of ribosomal RNA and genomic DNA from soil for studying the diversity of the indigenous bacterial community

  1998influential reference
• Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity

  1998cited by this paper
• Determination of microbial diversity in environmental samples: pitfalls of PCR-based rRNA analysis.

  1997cited by this paper
• Molecular microbial diversity in soils from eastern Amazonia: evidence for unusual microorganisms and microbial population shifts associated with deforestation

  1997cited by this paper
• Phylogenetic diversity of a bacterial community determined from Siberian tundra soil DNA.

  1997cited by this paper
• Rapid and direct method for extraction of RNA from soil

  1997cited by this paper
• DNA recovery from soils of diverse composition

  1996influential reference
• Bacterioplankton RNA, DNA, protein content and relationships to rates of thymidine and leucine incorporation

  1996cited by this paper
• Rapid Extraction of DNA and rRNA from Sediments by a Novel Hydroxyapatite Spin-Column Method

  1996cited by this paper
• Isolation and characterization of RNA from low-biomass deep-subsurface sediments

  1995influential reference
• Extraction and purification of PCR amplifiable DNA from lacustrine subsurface sediments

  1995cited by this paper
• Phylogenetic identification and in situ detection of individual microbial cells without cultivation.

  1995cited by this paper
• Improved Method for Recovery of mRNA from Aquatic Samples and Its Application to Detection of mer Expression

  1994cited by this paper
• Isolation, characterization, and distribution of denitrifying toluene degraders from a variety of habitats

  1994cited by this paper
• Effects of DNA Polymer Length on Its Adsorption to Soils

  1994cited by this paper
• Purification and molecular analysis of microbian DNA from compost

  1994cited by this paper
• Purification of DNA from estuarine sediments

  1994influential reference
• Comparison of Nucleic Acid Hybridization and Fluorometry for Measurement of the Relationship between RNA/DNA Ratio and Growth Rate in a Marine Bacterium

  1993cited by this paper
• Direct extraction and purification of rRNA for ecological studies

  1993cited by this paper
• Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast

  1993cited by this paper
• Quantitative Relationship between Naphthalene Catabolic Gene Frequency and Expression in Predicting PAH Degradation in Soils at Town Gas Manufacturing Sites

  1993cited by this paper
• Bacterial diversity in a soil sample from a subtropical Australian environment as determined by 16S rDNA analysis

  1993cited by this paper
• Gene Expression per Gene Dose, a Specific Measure of Gene Expression in Aquatic Microorganisms

  1993cited by this paper
• Detection and enumeration of bacteria in soil by direct DNA extraction and polymerase chain reaction

  1992cited by this paper
• Direct recovery and molecular analysis of DNA and RNA from soil.

  1992cited by this paper
• Rapid Method for Direct Extraction of mRNA from Seeded Soils

  1991cited by this paper
• Adsorption of plasmid DNA to mineral surfaces and protection against DNase I

  1991cited by this paper
• Rapid method for direct extraction of DNA from soil and sediments

  1991cited by this paper
• DNA Probe Method for the Detection of Specific Microorganisms in the Soil Bacterial Community

  1988cited by this paper
• DNA adsorption to soils and sediments.

  1988cited by this paper
• Recovery of DNA from soils and sediments

  1988cited by this paper
• Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.

  1987cited by this paper
• The extraction and purification of microbial DNA from sediments

  1987cited by this paper
• The adsorption of nucleic acids by montmorillonite

  1969cited by this paper
• METHODS OF MAKING MECHANICAL ANALYSES OF SOILS

  1949cited by this paper

• Cost-effective DNA extraction method optimized for high yield and long fragments from coastal sediments.

  2026cites this paper
• Effects of mineral resources on the growth of the harmful algal species Microcystis aeruginosa: From screening to understanding the underlying mechanisms.

  2025cites this paper
• Optimising RNA extraction for paddy bulk soil samples for metatranscriptome sequencing

  2025cites this paper
• Genomic profiling of soil nitrifying microorganisms enriched on floating membrane filter.

  2025cites this paper
• Assessment of rhizosphere microbial activity using optimized RNA extraction coupled with universal ribosomal RNA depletion techniques

  2025cites this paper
• Kinetic Plasticity of Nitrite-Oxidizing Bacteria Containing Cytoplasmic Nitrite Oxidoreductase

  2025cites this paper
• Long-term and seasonal evaluation on environmental microbiology and water quality of Shanmei reservoir in southeast China.

  2025cites this paper
• Comprehensive analysis of orthologous genes reveals functional dynamics and energy metabolism in the rhizospheric microbiome of Moringa oleifera

  2025cites this paper
• Enriched enzymes and crosstalking KEGG pathways in the rhizospheric soil fungiome of the wild plant Moringa oleifera.

  2025cites this paper
• Using bacterial and mitochondrial DNA markers to assess fecal pollution sources in stream water and sediments of a mixed land-use watershed.

  2025cites this paper
• Functional profiling of abundant glycosyltransferases in the rhizospheric bacteriome of Abutilon fruticosum

  2025cites this paper
• Exploration of genes encoding KEGG pathway enzymes in rhizospheric microbiome of the wild plant Abutilon fruticosum

  2024cites this paper
• Unraveling iron oxides as abiotic catalysts of organic phosphorus recycling in soil and sediment matrices

  2024cites this paper
• Profile of Bacterial Communities in Copper Mine Tailings Revealed through High-Throughput Sequencing

  2024influential citation
• Nitrous oxide respiration in acidophilic methanotrophs

  2024cites this paper
• Litter Removal Counteracts the Effects of Warming on Soil Bacterial Communities in the Qinghai–Tibet Plateau

  2024cites this paper
• Growth of soil ammonia-oxidizing archaea on air-exposed solid surface

  2024cites this paper
• Unique Features of Extremely Halophilic Microbiota Inhabiting Solar Saltworks Fields of Vietnam

  2024cites this paper
• Clearing the air: unraveling past and guiding future research in atmospheric chemosynthesis

  2023cites this paper
• DNA sequencing at the picogram level to investigate life on Mars and Earth

  2023cites this paper
• First shotgun metagenomics study of Juan de Fuca deep-sea sediments reveals distinct microbial communities above, within, between, and below sulfate methane transition zones

  2023cites this paper
• Comamonas-dominant microbial community in carbon poor aquitard sediments revealed by metagenomic-based growth rate investigation.

  2023cites this paper
• Microbial nitrogen and phosphorus co‐limitation across permafrost region

  2023cites this paper
• Methylacidiphilum caldifontis gen. nov., sp. nov., a thermoacidophilic methane-oxidizing bacterium from an acidic geothermal environment, and descriptions of the family Methylacidiphilaceae fam. nov. and order Methylacidiphilales ord. nov.

  2023cites this paper
• Functional analysis of bacterial genes accidentally packaged in rhizospheric phageome of the wild plant species Abutilon fruticosum

  2023cites this paper
• Functional annotation of rhizospheric phageome of the wild plant species Moringa oleifera

  2023cites this paper
• Abundant resistome determinants in rhizosphere soil of the wild plant Abutilon fruticosum

  2023cites this paper
• Function of CAZymes encoded by highly abundant genes in rhizosphere microbiome of Moringa oleifera

  2023cites this paper
• Methylococcus mesophilus sp. nov., the first non-thermotolerant methanotroph of the genus Methylococcus, from a rice field.

  2023cites this paper
• Metatranscriptomic analysis of tomato rhizospheres reveals insight into plant-microbiome molecular response to biochar-amended organic soil

  2023cites this paper
• Abundant antibiotic resistance genes in rhizobiome of the human edible Moringa oleifera medicinal plant

  2022cites this paper
• Microbiome structure and response to watering in rhizosphere of Nitrosalsola vermiculata and surrounding bulk soil

  2022cites this paper
• Selectivity in Enzymatic Phosphorus Recycling from Biopolymers: Isotope Effect, Reactivity Kinetics, and Molecular Docking with Fungal and Plant Phosphatases

  2022cites this paper
• The nature of gas production patterns associated with methanol degradation in natural aquifer sediments: A microcosm study.

  2022cites this paper
• Functional Interpretation of Cross-Talking Pathways with Emphasis on Amino Acid Metabolism in Rhizosphere Microbiome of the Wild Plant Moringa oleifera

  2022cites this paper
• Climate warming enhances microbial network complexity and stability

  2021cites this paper
• Improvements in Extraction Methods of High-molecular-weight DNA from Soils by Modifying Cell Lysis Conditions and Reducing Adsorption of DNA onto Soil Particles

  2021cites this paper
• Metagenomic Bioprospecting of Uncultivable Microbial Flora in Soil Microbiome for Novel Enzymes

  2021cites this paper
• Application of Soil Microorganisms for Agricultural and Environmental Sustainability: A Review

  2021cites this paper
• Oyster Biodeposition Alleviates Sediment Nutrient Overload: A Case Study at Shenzhen Bay, China

  2021cites this paper
• Development and validation of a flexible DNA extraction (PAN) method for liquid biopsy of multiple sample types

  2021cites this paper
• Discovery and characterization of a novel protease from the Antarctic soil

  2021cites this paper
• An improved method for extraction of microbial DNA from alkaline-saline soil

  2021cites this paper
• Sedimentary Ancient DNA (sedaDNA) Reveals Fungal Diversity and Environmental Drivers of Community Changes throughout the Holocene in the Present Boreal Lake Lielais Svētiņu (Eastern Latvia)

  2021cites this paper
• Active Microbiome Structure and Functional Analyses of Freshwater Benthic Biofilm Samples Influenced by RNA Extraction Methods

  2021cites this paper
• Verrucomicrobial methanotrophs grow on diverse C3 compounds and use a homolog of particulate methane monooxygenase to oxidize acetone

  2021cites this paper
• Nitrogen recovery by a halophilic ammonium-assimilating microbiome: A new strategy for saline wastewater treatment.

  2021cites this paper
• The guanidine thiocyanate‐high EDTA method for total microbial RNA extraction from severely heavy metal‐contaminated soils

  2020influential citation
• Rhizosphere fungi actively assimilating plant-derived carbon in a grassland soil

  2020cites this paper
• An updated review on bacterial community composition of traditional fermented milk products: what next-generation sequencing has revealed so far?

  2020cites this paper
• Improved Protocol for DNA Extraction from Subsoils Using Phosphate Lysis Buffer

  2020cites this paper
• Microbial Functional Diversity in Vineyard Soils: Sulfur Metabolism and Links With Grapevine Plants and Wine Quality

  2020cites this paper
• Bayesian Sparse Multivariate Regression with Asymmetric Nonlocal Priors for Microbiome Data Analysis

  2020cites this paper
• The mercury-tolerant microbiota of the zooplankton Daphnia aids in host survival and maintains fecundity under mercury stress.

  2019cites this paper
• Swift evolutionary response of microbes to a rise in anthropogenic mercury in the Northern Hemisphere

  2019cites this paper
• Metagenomic insights into the diversity and functions of microbial assemblages in lakes

  2019cites this paper
• Phylogenetic Techniques in Geomicrobiology

  2019cites this paper
• Microbial Degradation of Cellulosic Material and Gas Generation: Implications for the Management of Low- and Intermediate-Level Radioactive Waste

  2019cites this paper
• Cumulative impact of long-term intensive mariculture on total and active bacterial communities in the core sediments of the Ailian Bay, North China.

  2019cites this paper
• Genomic and metatranscriptomic analyses of carbon remineralization in an Antarctic polynya

  2019cites this paper
• Contrasting histories of microcystin-producing cyanobacteria in two temperate lakes as inferred from quantitative sediment DNA analyses

  2019cites this paper
• Differential ratio amplicons (R amp) for the evaluation of RNA integrity extracted from complex environmental samples

  2019cites this paper
• Microorganisms: an asset for decontamination of soil

  2019cites this paper
• General Geochemistry and Microbiology Techniques

  2019cites this paper
• Alive and kicking: Why dormant soil microorganisms matter

  2018cites this paper
• Co-selection of metal and antibiotic resistance in the gut microbial community of the Mummichog fish (Fundulus heteroclitus)

  2018cites this paper
• Differential amplicons for the evaluation of RNA integrity extracted from complex environmental samples

  2018cites this paper
• INFLUENCE OF MICROBIAL DEGRADATION ON GAS PRESSURE EVOLUTION AND TERMINAL GAS COMPOSITION IN BATCH ANAEROBIC CONTAINERS OF SURROGATE LOW-TO-INTERMEDIATE LEVEL RADIOACTIVE WASTE

  2018cites this paper
• Characterizing the Deep Terrestrial Subsurface Microbiome.

  2018cites this paper
• Functional Transcripts Indicate Phylogenetically Diverse Active Ammonia-Scavenging Microbiota in Sympatric Sponges

  2018cites this paper
• Sterilization impacts on marine sediment---Are we able to inactivate microorganisms in environmental samples?

  2018cites this paper
• Substratum‐Associated Microbiota

  2018cites this paper
• Chapter 13 – Metagenomics of Marine Invertebrate-Microbial Consortium

  2018cites this paper
• Shift from dormancy to microbial growth revealed by RNA:DNA ratio

  2018cites this paper
• Isolation of high molecular weight and humic acid-free metagenomic DNA from lignocellulose-rich samples compatible for direct fosmid cloning

  2018cites this paper
• Variation of bacterial biodiversity from saline soils and estuary sediments present near the Mediterranean Sea coast of Camargue (France)

  2018cites this paper
• Environmental DNA (eDNA) and environmental RNA (eRNA) markers for detection of grass carp (Ctenopharyngodon idella)

  2018cites this paper
• Soil CO2 emission partitioning, bacterial community profile and gene expression of Nitrosomonas spp. and Nitrobacter spp. of a sandy soil amended with biochar and compost

  2017cites this paper
• In Situ Expression of nigD in Geobacteraceae in Subsurface Sediments

  2017cites this paper
• The choice of the DNA extraction method may influence the outcome of the soil microbial community structure analysis

  2017cites this paper
• Loughborough University Institutional Repository Microbiology of wind-eroded sediments : current knowledge and future research directions

  2017cites this paper
• Environmental Bioremediation: Biodegradation of Xenobiotic Compounds

  2017cites this paper
• HIGH-QUALITY DNA FROM PEAT SOIL FOR METAGENOMIC STUDIES: A MINIREVIEW ON DNA EXTRACTION METHODS

  2017cites this paper
• Cyanobacteria North of 60°: Environmental DNA Approaches

  2017cites this paper
• Nucleic Acid Extraction for Studying Plant-Microbe Interactions in Rhizosphere

  2017cites this paper
• Alterations in preconception, antenatal, and postnatal maternal gut microbiota influence offspring intestinal microbiota and immunity

  2017cites this paper
• A simple method for large-scale purification of nucleic acids from plants using calcium phosphate-type monetite

  2017cites this paper
• Changes in Soil Microbial Community Structure Associated with Iron Reduction and Phosphate Release in Soil Microcosms

  2017influential citation
• Co-selection of Mercury and Multiple Antibiotic Resistances in Bacteria Exposed to Mercury in the Fundulus heteroclitus Gut Microbiome

  2016cites this paper
• The Role of Microbes in Plastic Degradation

  2016cites this paper
• Estimates of Soil Bacterial Ribosome Content and Diversity Are Significantly Affected by the Nucleic Acid Extraction Method Employed

  2016cites this paper
• Isolation of Pure DNA for Metagenomic Study from Industrial Polluted Sites: A New Approach for Monitoring the Microbial Community and Pollutants

  2016cites this paper
• Sample Preparation for Bioanalytical and Pharmaceutical Analysis.

  2016cites this paper
• Benthic protists: the under-charted majority.

  2016cites this paper
• Preparation of Nucleic Acids from Marine Samples: Applications to Microbial Ecology Research

  2016cites this paper
• Quantifying the biases in metagenome mining for realistic assessment of microbial ecology of naturally fermented foods

  2016cites this paper
• Interaction Between Pesticide and Soil Microorganisms and Their Degradation: A Molecular Approach

  2016cites this paper
• Warming Alters Expressions of Microbial Functional Genes Important to Ecosystem Functioning

  2016cites this paper
• Sample Preparation Techniques for Soil, Plant, and Animal Samples

  2016cites this paper
• Cytochrome P450 monooxygenase specific activity reduction in wheat Triticum aestivum induced by soil roxithromycin stress

  2016cites this paper