Control of somatic embryogenesis and embryo development by AP2 transcription factors

Members of the AP2 family of transcription factors, such as BABY BOOM (BBM), play important roles in cell proliferation and embryogenesis in Arabidopsis thaliana (AtBBM) and Brassica napus (BnBBM) but how this occurs is not understood. We have isolated three AP2 genes (GmBBM1, GmAIL5, GmPLT2) from somatic embryo cultures of soybean, Glycine max (L.) Merr, and discovered GmBBM1 to be homologous to AtBBM and BnBBM. GmAIL5 and GmPLT2 were homologous to Arabidopsis AINTEGUMENTA-like5 (AIL5) and PLETHORA2 (PLT2), respectively. Constitutive expression of GmBBM1 in Arabidopsis induced somatic embryos on vegetative organs and other pleiotropic effects on post-germinative vegetative organ development. Sequence comparisons of BBM orthologues revealed the presence of ten sequence motifs outside of the AP2 DNA-binding domains. One of the motifs, bbm-1, was specific to the BBM-like genes. Deletion and domain swap analyses revealed that bbm-1 was important for somatic embryogenesis and acted cooperatively with at least one other motif, euANT2, in the regulation of somatic embryogenesis and embryo development in transgenic Arabidopsis. The results provide new insights into the mechanisms by which BBM governs embryogenesis.

• Publication year

  2010

• Venue

  Plant Molecular Biology

• Publication date

  2010-08-27

• Fields of study

  Biology, Medicine, Environmental Science

• Identifiers
  DOI 10.1007/s11103-010-9674-8PMID 20798978PMCID 2952763
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Arabidopsis EMBRYOMAKER encoding an AP2 domain transcription factor plays a key role in developmental change from vegetative to embryonic phase

  2010influential reference
• Molecular Evolution and Phylogenetics

  2008cited by this paper
• Phylogeny, gene structures, and expression patterns of the ERF gene family in soybean (Glycine max L.)

  2008cited by this paper
• BABY BOOM target genes provide diverse entry points into cell proliferation and cell growth pathways

  2008cited by this paper
• Transcriptional profiling of Medicago truncatula meristematic root cells

  2008cited by this paper
• PLETHORA proteins as dose-dependent master regulators of Arabidopsis root development

  2007influential reference
• The Ancestral Developmental Tool Kit of Land Plants

  2007influential reference
• Pluripotent versus totipotent plant stem cells: dependence versus autonomy?

  2007cited by this paper
• EgAP2-1, an AINTEGUMENTA-like (AIL) gene expressed in meristematic and proliferating tissues of embryos in oil palm

  2007cited by this paper
• Factors involved in root formation in Medicago truncatula.

  2006influential reference
• Genome-Wide Analysis of the ERF Gene Family in Arabidopsis and Rice[W]

  2006cited by this paper
• Phylogeny and domain evolution in the APETALA2-like gene family.

  2006influential reference
• Heterologous expression of the BABY BOOM AP2/ERF transcription factor enhances the regeneration capacity of tobacco (Nicotiana tabacum L.)

  2006influential reference
• ProtTest: selection of best-fit models of protein evolution

  2005influential reference
• An Annotation Update via cDNA Sequence Analysis and Comprehensive Profiling of Developmental, Hormonal or Environmental Responsiveness of the Arabidopsis AP2/EREBP Transcription Factor Gene Family

  2005cited by this paper
• APETALA2 Regulates the Stem Cell Niche in the Arabidopsis Shoot Meristem[W]

  2005cited by this paper
• Control of seed mass by APETALA2.

  2005cited by this paper
• AINTEGUMENTA-like (AIL) genes are expressed in young tissues and may specify meristematic or division-competent states

  2005influential reference
• Journal of Experimental Botany, Page 1 of 13

  2005influential reference
• Development of an embryogenic suspension culture of soybean (Glycine max Merrill.)

  2004influential reference
• The PLETHORA genes mediate patterning of the Arabidopsis root stem cell niche.

  2004influential reference
• Genome-Wide ORFeome Cloning and Analysis of Arabidopsis Transcription Factor Genes1[w]

  2004cited by this paper
• DNA-binding specificity of the ERF/AP2 domain of Arabidopsis DREBs, transcription factors involved in dehydration- and cold-inducible gene expression.

  2002cited by this paper
• Ectopic Expression of BABY BOOM Triggers a Conversion from Vegetative to Embryonic Growth Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.001941.

  2002influential reference
• Genotype screening for proliferative embryogenesis and biolistic transformation of short-season soybean genotypes

  2000influential reference
• Plant organ size control: AINTEGUMENTA regulates growth and cell numbers during organogenesis.

  2000cited by this paper
• AINTEGUMENTA Promotes Petal Identity and Acts as a Negative Regulator of AGAMOUS

  2000influential reference
• Arabidopsis transcription factors: genome-wide comparative analysis among eukaryotes.

  2000cited by this paper
• The inflorescence architecture of Petunia hybrida is modified by the Arabidopsis thaliana Ap2 gene.

  1999cited by this paper
• The AP2/EREBP family of plant transcription factors.

  1998cited by this paper
• Floral dip: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana.

  1998cited by this paper
• The AP2 domain of APETALA2 defines a large new family of DNA binding proteins in Arabidopsis.

  1997cited by this paper
• GeneDoc: a tool for editing and annotating multiple sequence alignments

  1997influential reference
• AINTEGUMENTA, an APETALA2-like gene of Arabidopsis with pleiotropic roles in ovule development and floral organ growth.

  1996cited by this paper
• The AINTEGUMENTA gene of Arabidopsis required for ovule and female gametophyte development is related to the floral homeotic gene APETALA2.

  1996cited by this paper
• Automated construction and graphical presentation of protein blocks from unaligned sequences.

  1995influential reference
• Fitting a Mixture Model By Expectation Maximization To Discover Motifs In Biopolymer

  1994influential reference
• Control of Arabidopsis flower and seed development by the homeotic gene APETALA2.

  1994cited by this paper
• CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.

  1994influential reference
• Apical proliferation of embryogenic tissue of soybean [Glycine max (L.) Merrill]

  1988cited by this paper

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