GmMYB181, a Soybean R2R3-MYB Protein, Increases Branch Number in Transgenic Arabidopsis

Soybean (Glycine max) is an important economic crop that provides abundant oil and high quality protein for human beings. As the process of reproductive growth directly determines the crop seed yield and quality, we initiated studies to identify genes that regulate soybean floral organ development. One R2R3-MYB transcription factor gene, designated as GmMYB181, was found to be enriched in flowers based on microarray analysis and was further functionally investigated in transgenic Arabidopsis. GmMYB181 protein contains two MYB domains, which localized to the nucleus and displayed transcriptional activation in yeast hybrid system. Real-time quantitative PCR (qRT-PCR) results suggested GmMYB181 exclusively expressed in flower tissue. In Arabidopsis, overexpression of GmMYB181 altered the morphology of floral organs, fruit size and plant architecture, including outward curly sepals, smaller siliques, increased lateral branches and reduced plant height, indicating that GmMYB181 is involved in the development of reproductive organs and plays an important role in controlling plant architecture. Further, microarray analysis revealed that overexpressing GmMYB181 in Arabidopsis affected the expression of 3450 genes in mature flowers, including those involved in floral organ, seed/fruit development, and responded to different hormone signals.

• Publication year

  2018

• Venue

  Frontiers in Plant Science

• Publication date

  2018-07-17

• Fields of study

  Biology, Agricultural and Food Sciences, Medicine, Environmental Science

• Identifiers
  DOI 10.3389/fpls.2018.01027PMID 30065741PMCID 6056663
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• BjMYB1, a transcription factor implicated in plant defence through activating BjCHI1 chitinase expression by binding to a W-box-like element

  2016cited by this paper
• Opposing Control by Transcription Factors MYB61 and MYB3 Increases Freezing Tolerance by Relieving C-Repeat Binding Factor Suppression1[OPEN]

  2016cited by this paper
• Organ boundary NAC-domain transcription factors are implicated in the evolution of petal fusion.

  2016cited by this paper
• Cytokinin response factor 4 (CRF4) is induced by cold and involved in freezing tolerance

  2015cited by this paper
• Pea VEGETATIVE2 Is an FD Homolog That Is Essential for Flowering and Compound Inflorescence Development

  2015cited by this paper
• Soybean GmMYB73 promotes lipid accumulation in transgenic plants

  2014cited by this paper
• A novel MYB transcription factor, GmMYBJ1, from soybean confers drought and cold tolerance in Arabidopsis thaliana.

  2014cited by this paper
• A soybean MADS-box protein modulates floral organ numbers, petal identity and sterility

  2014cited by this paper
• The Arabidopsis cysteine-rich GASA5 is a redox-active metalloprotein that suppresses gibberellin responses.

  2014cited by this paper
• The tomato NAC transcription factor SlNAM2 is involved in flower-boundary morphogenesis

  2013cited by this paper
• An apple MYB transcription factor, MdMYB3, is involved in regulation of anthocyanin biosynthesis and flower development

  2013cited by this paper
• A MYB transcription factor controls flower color in soybean.

  2013cited by this paper
• ABA and the ubiquitin E3 ligase KEEP ON GOING affect proteolysis of the Arabidopsis thaliana transcription factors ABF1 and ABF3

  2013cited by this paper
• Biogenesis, Turnover, and Mode of Action of Plant MicroRNAs[OPEN]

  2013cited by this paper
• A cotton gene encoding MYB-like transcription factor is specifically expressed in pollen and is involved in regulation of late anther/pollen development.

  2013cited by this paper
• Isolation of the floral morph-related genes in heterostylous flax (Linum grandiflorum): the genetic polymorphism and the transcriptional and post-transcriptional regulations of the S locus.

  2012influential reference
• WOX2 and STIMPY-LIKE/WOX8 promote cotyledon boundary formation in Arabidopsis.

  2012cited by this paper
• Arabidopsis AtNAP regulates fruit senescence

  2012cited by this paper
• Genome-wide analysis of the MYB transcription factor superfamily in soybean

  2012cited by this paper
• The R2R3 MYB Transcription Factors FOUR LIPS and MYB88 Regulate Female Reproductive Development

  2012cited by this paper
• Genome-wide classification and expression analysis of MYB transcription factor families in rice and Arabidopsis

  2012cited by this paper
• Loss-of-function mutations affecting a specific Glycine max R2R3 MYB transcription factor result in brown hilum and brown seed coats

  2011cited by this paper
• The Jasmonate-ZIM Domain Proteins Interact with the R2R3-MYB Transcription Factors MYB21 and MYB24 to Affect Jasmonate-Regulated Stamen Development in Arabidopsis[C][W]

  2011influential reference
• Floral-dip transformation of Arabidopsis thaliana to examine pTSO2::beta-glucuronidase reporter gene expression.

  2010cited by this paper
• Control of somatic embryogenesis and embryo development by AP2 transcription factors

  2010cited by this paper
• The Arabidopsis MYB5 Transcription Factor Regulates Mucilage Synthesis, Seed Coat Development, and Trichome Morphogenesis[W][OA]

  2009cited by this paper
• Subcellular homeostasis of phytohormone auxin is mediated by the ER-localized PIN5 transporter

  2009cited by this paper
• Overexpression of a grapevine R2R3-MYB factor in tomato affects vegetative development, flower morphology and flavonoid and terpenoid metabolism.

  2009cited by this paper
• A Novel Fatty Acyl-CoA Synthetase Is Required for Pollen Development and Sporopollenin Biosynthesis in Arabidopsis[C][W]

  2009cited by this paper
• Gibberellin Acts through Jasmonate to Control the Expression of MYB21, MYB24, and MYB57 to Promote Stamen Filament Growth in Arabidopsis

  2009cited by this paper
• STAMENLESS 1, encoding a single C2H2 zinc finger protein, regulates floral organ identity in rice.

  2009cited by this paper
• SHORT HYPOCOTYL UNDER BLUE1 Associates with MINISEED3 and HAIKU2 Promoters in Vivo to Regulate Arabidopsis Seed Development[W]

  2009cited by this paper
• Identification of transcription factors predominantly expressed in soybean flowers and characterization of GmSEP1 encoding a SEPALLATA1-like protein.

  2009cited by this paper
• Analysis of the grape MYB R2R3 subfamily reveals expanded wine quality-related clades and conserved gene structure organization across Vitis and Arabidopsis genomes

  2008influential reference
• MYB108 Acts Together with MYB24 to Regulate Jasmonate-Mediated Stamen Maturation in Arabidopsis1[OA]

  2008cited by this paper
• Role of the Arabidopsis thaliana NAC transcription factors ANAC019 and ANAC055 in regulating jasmonic acid-signaled defense responses

  2008cited by this paper
• Overexpression of the non-canonical Aux/IAA genes causes auxin-related aberrant phenotypes in Arabidopsis.

  2008cited by this paper
• The BLADE-ON-PETIOLE genes are essential for abscission zone formation in Arabidopsis

  2008cited by this paper
• Over-expression of a flower-specific transcription factor gene AtMYB24 causes aberrant anther development

  2007influential reference
• The transcription factor HIG1/MYB51 regulates indolic glucosinolate biosynthesis in Arabidopsis thaliana.

  2007cited by this paper
• XND1, a member of the NAC domain family in Arabidopsis thaliana, negatively regulates lignocellulose synthesis and programmed cell death in xylem.

  2007cited by this paper
• CYP703 Is an Ancient Cytochrome P450 in Land Plants Catalyzing in-Chain Hydroxylation of Lauric Acid to Provide Building Blocks for Sporopollenin Synthesis in Pollen[W]

  2007cited by this paper
• Genome-wide analysis of Agrobacterium T-DNA integration sites in the Arabidopsis genome generated under non-selective conditions.

  2007cited by this paper
• Characterization of a novel putative zinc finger gene MIF1: involvement in multiple hormonal regulation of Arabidopsis development.

  2006cited by this paper
• Blind Homologous R2R3 Myb Genes Control the Pattern of Lateral Meristem Initiation in Arabidopsis[W]

  2006cited by this paper
• BIGPETALp, a bHLH transcription factor is involved in the control of Arabidopsis petal size

  2006cited by this paper
• Overexpression of INFLORESCENCE DEFICIENT IN ABSCISSION Activates Cell Separation in Vestigial Abscission Zones in Arabidopsis[OA]

  2006cited by this paper
• The Arabidopsis GAMYB-Like Genes, MYB33 and MYB65, Are MicroRNA-Regulated Genes That Redundantly Facilitate Anther Development

  2005cited by this paper
• MINISEED3 (MINI3), a WRKY family gene, and HAIKU2 (IKU2), a leucine-rich repeat (LRR) KINASE gene, are regulators of seed size in Arabidopsis.

  2005cited by this paper
• Molecular mechanisms of flower development: an armchair guide

  2005cited by this paper
• Maternal Control of Integument Cell Elongation and Zygotic Control of Endosperm Growth Are Coordinated to Determine Seed Size in Arabidopsis

  2005cited by this paper
• Shoot and inflorescence branching.

  2005cited by this paper
• The Biochemical Characterization of Two Carotenoid Cleavage Enzymes from Arabidopsis Indicates That a Carotenoid-derived Compound Inhibits Lateral Branching*

  2004cited by this paper
• A role for HvGAMYB in anther development.

  2003cited by this paper
• Effects of genomic position and copy number of Acyl-ACP thioesterase transgenes on the level of the target fatty acids in Brassica napus L.

  2003cited by this paper
• MYB transcription factors in Arabidopsis.

  2002cited by this paper
• The tomato Blind gene encodes a MYB transcription factor that controls the formation of lateral meristems

  2002cited by this paper
• MYB61 Is Required for Mucilage Deposition and Extrusion in the Arabidopsis Seed Coat Article, publication date, and citation information can be found at www.plantcell.org/cgi/doi/10.1105/tpc.010265.

  2001cited by this paper
• Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) Method.

  2001cited by this paper
• Gene activation and gene silencing.

  2001cited by this paper
• Gene stability in transgenic aspen (Populus). II. Molecular characterization of variable expression of transgene in wild and hybrid aspen

  2001cited by this paper
• B and C floral organ identity functions require SEPALLATA MADS-box genes

  2000cited by this paper
• Towards functional characterisation of the members of the R2R3-MYB gene family from Arabidopsis thaliana.

  1998cited by this paper
• The ArabidopsisAGL9 MADS box gene is expressed in young flower primordia

  1998cited by this paper
• THE SILENCE OF GENES IN TRANSGENIC PLANTS

  1997cited by this paper
• Review Article: The Silence of Genes in Transgenic Plants

  1997cited by this paper
• A novel class of MADS box genes is involved in ovule development in petunia.

  1995cited by this paper

• Identifying Candidate Genes Related to the Nutritional Components of Soybean (Glycine max) Sprouts Based on the Transcriptome and Co-Expression Network

  2025cites this paper
• Identification of QTL for branch traits in soybean (Glycine max L.) and its application in genomic selection

  2025cites this paper
• A systematic regulatory network related to bulbil formation in Lilium lancifolium based on metabolome and transcriptome analyses

  2024cites this paper
• Identification of an IAA conjugate resistant gene BraA07g034950.3C regulating primary rosette branching in flowering Chinese cabbage

  2024cites this paper
• Molecular and genetic basis of plant architecture in soybean

  2024cites this paper
• Molecular Regulation of Shoot Architecture in Soybean.

  2024cites this paper
• Soybean flower-specific R2R3-MYB transcription factor gene GmMYB108 induces anthocyanin production in Arabidopsis thaliana

  2024cites this paper
• OfBFT genes play an essential role in the proliferate flower formation of Osmanthus fragrans.

  2024cites this paper
• A Soybean Pyrroline-5-Carboxylate Dehydrogenase GmP5CDH1 Modulates Plant Growth and Proline Sensitivity

  2024cites this paper
• Insights into the Genetic Determination of the Autotetraploid Potato Plant Height

  2023cites this paper
• Mechanisms underlying key agronomic traits and implications for molecular breeding in soybean.

  2023cites this paper
• A single nucleotide mutation in ClphyB gene is associated with a short lateral branch phenotype in watermelon

  2023cites this paper
• The Role of GmSnRK1-GmNodH Module in Regulating Soybean Nodulation Capacity

  2023cites this paper
• MYB transcription factors and their role in Medicinal plants

  2022cites this paper
• Cloning and Functional Verification of CmRAX2 Gene Associated with Chrysanthemum Lateral Branches Development

  2022cites this paper
• Overexpression of AtMYB115 gene in transgenic rice exhibits increased early regeneration and results in dwarf phenotype

  2022cites this paper
• GsMYB7 encoding a R2R3-type MYB transcription factor enhances the tolerance to aluminum stress in soybean (Glycine max L.)

  2022cites this paper
• An enhancing effect attributed to a nonsynonymous mutation in SOYBEAN SEED SIZE 1, a SPINDLY-like gene, is exploited in soybean domestication and improvement.

  2022cites this paper
• Functional Analysis of ScABI3 from Syntrichia caninervis Mitt. in Medicago sativa L.

  2022cites this paper
• PhMYB37 Promotes Shoot Branching in Petunia

  2022cites this paper
• Identification of two tandem genes associated with primary rosette branching in flowering Chinese cabbage

  2022influential citation
• The DNA Replication, Repair, and Recombination Pathway Genes Modulating Yield and Stress Tolerance Traits in Chickpea

  2021cites this paper
• The DNA Replication, Repair, and Recombination Pathway Genes Modulating Yield and Stress Tolerance Traits in Chickpea

  2021cites this paper
• Nondestructive phenotyping fatty acid trait of single soybean seeds using reflective hyperspectral imagery

  2021cites this paper
• Systematic analysis of differentially expressed ZmMYB genes related to drought stress in maize

  2021cites this paper
• Overexpression of GmGAMYB Accelerates the Transition to Flowering and Increases Plant Height in Soybean

  2021cites this paper
• Physical mapping and candidate gene prediction of branch number on the main stem in soybean [Glycine max (L.) Merr.]

  2021cites this paper
• Plant transcription factors in responses to abiotic stressors: MYB proteins

  2020cites this paper
• Ectopic expression of a R2R3 MYB transcription factor of dove tree (Davidia involucrata) aggravates seed abortion in Arabidopsis thaliana.

  2020cites this paper
• Overexpression of GmMYB14 improves high‐density yield and drought tolerance of soybean through regulating plant architecture mediated by the brassinosteroid pathway

  2020cites this paper
• Genome-wide DNA methylation analysis of paulownia with phytoplasma infection.

  2020cites this paper
• Characterization of the soybean R2R3-MYB transcription factor GmMYB81 and its functional roles under abiotic stresses.

  2020cites this paper
• Phylogenomic Analysis of R2R3 MYB Transcription Factors in Sorghum and their Role in Conditioning Biofuel Syndrome

  2020cites this paper
• Comparative transcriptome and coexpression network analysis of carpel quantitative variation in Paeonia rockii

  2019cites this paper
• Quantitative trait locus mapping for plant height and branch number in an upland cotton recombinant inbred line with an SNP-based high-density genetic map

  2019cites this paper
• Fine mapping and identification of ub4 as a candidate gene associated with tassel branch number in maize (Zea mays L.)

  2019cites this paper