Efficient CRISPR/Cas9-mediated genome editing in P. falciparum

Malaria is a major cause of global morbidity and mortality, and new strategies for treating and preventing this disease are needed. Here we show that the Streptococcus pyogenes Cas9 DNA endonuclease and single guide RNAs (sgRNAs) produced using T7 RNA polymerase (T7 RNAP) efficiently edit the Plasmodium falciparum genome. Targeting the genes encoding native knob-associated histidine-rich protein (kahrp) and erythrocyte binding antigen 175 (eba-175), we achieved high (≥50–100%) gene disruption frequencies within the usual time frame for generating transgenic parasites.

• Publication year

  2014

• Venue

  Nature Methods

• Publication date

  2014-07-16

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/nmeth.3063PMID 25108687PMCID 4199390
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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