Accurate H3K27 methylation can be established de novo by SUZ12-directed PRC2

Polycomb repressive complex 2 (PRC2) catalyzes methylation on lysine 27 of histone H3 (H3K27) and is required for maintaining transcriptional patterns and cellular identity, but the specification and maintenance of genomic PRC2 binding and H3K27 methylation patterns remain incompletely understood. Epigenetic mechanisms have been proposed, wherein pre-existing H3K27 methylation directs recruitment and regulates the catalytic activity of PRC2 to support its own maintenance. Here we investigate whether such mechanisms are required for specifying H3K27 methylation patterns in mouse embryonic stem cells (mESCs). Through re-expression of PRC2 subunits in PRC2-knockout cells that have lost all H3K27 methylation, we demonstrate that methylation patterns can be accurately established de novo. We find that regional methylation kinetics correlate with original methylation patterns even in their absence, and specification of the genomic PRC2 binding pattern is retained and specifically dependent on the PRC2 core subunit SUZ12. Thus, the H3K27 methylation patterns in mESCs are not dependent on self-autonomous epigenetic inheritance. Although mechanisms wherein pre-existing H3K27 methylation directs recruitment of PRC2 to support its own maintenance have been proposed, H3K27 methylation patterns in mESCs are not dependent on self-autonomous epigenetic inheritance.

• Publication year

  2018

• Venue

  Nature Structural & Molecular Biology

• Publication date

  2018-01-29

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1038/s41594-018-0036-6PMID 29483650PMCID 5842896
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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