Genome‐modification technologies enable the rational engineering and perturbation of biological systems. Historically, these methods have been limited to gene insertions or mutations at random or at a few pre‐defined locations across the genome. The handful of methods capable of targeted gene editing suffered from low efficiencies, significant labor costs, or both. Recent advances have dramatically expanded our ability to engineer cells in a directed and combinatorial manner. Here, we review current technologies and methodologies for genome‐scale engineering, discuss the prospects for extending efficient genome modification to new hosts, and explore the implications of continued advances toward the development of flexibly programmable chasses, novel biochemistries, and safer organismal and ecological engineering.
Genome-scale engineering for systems and synthetic biology
Published 2013 in Molecular Systems Biology
ABSTRACT
PUBLICATION RECORD
- Publication year
2013
- Venue
Molecular Systems Biology
- Publication date
2013-01-22
- Fields of study
Biology, Medicine, Engineering
- Identifiers
- External record
- Source metadata
Semantic Scholar, PubMed
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