MicroRNA-362 induces cell proliferation and apoptosis resistance in gastric cancer by activation of NF-κB signaling

BackgroundAccording to cancer-related microRNA (miRNA) expression microarray research available in public databases, miR-362 expression is elevated in gastric cancer. However, the expression and biological role of miR-362 in gastric progression remain unclear.MethodsmiR-362 expression levels in gastric cancer tissues and cell lines were determined using real-time PCR. The roles of miR-362, in promoting gastric cancer cell proliferation and apoptosis resistance, were assessed by different biological assays, such as colony assay, flow cytometry and TUNEL assay. The effect of miR-362 on NF-κB activation was investigated using the luciferase reporter assay, fluorescent immunostaining.ResultsMiR-362 overexpression induced cell proliferation, colony formation, and resistance to cisplatin-induced apoptosis in BGC-823 and SGC-7901 gastric cancer cells. MiR-362 increased NF-κB activity and relative mRNA expression of NF-κB–regulated genes, and induced nuclear translocation of p65. Expression of the tumor suppressor CYLD was inhibited by miR-362 in gastric cancer cells; miR-362 levels were inversely correlated with CYLD expression in gastric cancer tissue. MiR-362 downregulated CYLD expression by binding its 3′ untranslated region. NF-κB activation was mechanistically associated with siRNA-mediated downregulation of CYLD. MiR-362 inhibitor reversed all the effects of miR-362.ConclusionThe results suggest that miR-362 plays an important role in repressing the tumor suppressor CYLD and present a novel mechanism of miRNA-mediated NF-κB activation in gastric cancer.

• Publication year

  2014

• Venue

  Journal of Translational Medicine

• Publication date

  2014-02-05

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1186/1479-5876-12-33PMID 24495516PMCID 3916099
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Epidemiology of Gastric Cancer

  2015cited by this paper
• microRNA-372 maintains oncogene characteristics by targeting TNFAIP1 and affects NFκB signaling in human gastric carcinoma cells.

  2013cited by this paper
• miR-21 confers cisplatin resistance in gastric cancer cells by regulating PTEN.

  2013cited by this paper
• miR-19a promotes cell growth and tumorigenesis through targeting SOCS1 in gastric cancer.

  2013cited by this paper
• The Role of microRNA in Gastric Malignancy

  2013cited by this paper
• Plumbagin inhibits cell growth and potentiates apoptosis in human gastric cancer cells in vitro through the NF-κB signaling pathway

  2012cited by this paper
• Adjuvant capecitabine and oxaliplatin for gastric cancer after D2 gastrectomy (CLASSIC): a phase 3 open-label, randomised controlled trial.

  2012cited by this paper
• [Chronic inflammation and gastric cancer development].

  2012cited by this paper
• TGF-β induces miR-182 to sustain NF-κB activation in glioma subsets.

  2012cited by this paper
• Effect of NF-κB inhibitors on the chemotherapy-induced apoptosis of the colon cancer cell line HT-29

  2012cited by this paper
• GLI1-dependent transcriptional repression of CYLD in basal cell carcinoma

  2011cited by this paper
• Expanding role of ubiquitination in NF-κB signaling

  2011cited by this paper
• MicroRNA signatures differentiate melanoma subtypes

  2011cited by this paper
• Relation between microRNA expression and progression and prognosis of gastric cancer: a microRNA expression analysis.

  2010cited by this paper
• Down-regulation of CYLD as a trigger for NF-κB activation and a mechanism of apoptotic resistance in hepatocellular carcinoma cells.

  2010cited by this paper
• CYLD: a tumor suppressor deubiquitinase regulating NF-κB activation and diverse biological processes

  2010cited by this paper
• The Notch/Hes1 pathway sustains NF-κB activation through CYLD repression in T cell leukemia.

  2010cited by this paper
• Ubiquitin Conjugation and Deconjugation in NF-κB Signaling.

  2010cited by this paper
• Signaling to NF-kappaB: regulation by ubiquitination.

  2010cited by this paper
• Differential expression of microRNA species in human gastric cancer versus non‐tumorous tissues

  2009cited by this paper
• Down-regulation of CYLD expression by Snail promotes tumor progression in malignant melanoma

  2009cited by this paper
• MicroRNAs in cancer.

  2009cited by this paper
• Involvement of linear polyubiquitylation of NEMO in NF-κB activation

  2009cited by this paper
• MicroRNAs in Cancer.

  2009cited by this paper
• Astrocyte Elevated Gene-1 is a Novel Prognostic Marker for Breast Cancer Progression and Overall Patient Survival

  2008cited by this paper
• miR‐15b and miR‐16 modulate multidrug resistance by targeting BCL2 in human gastric cancer cells

  2008cited by this paper
• NF-KappaB expression correlates with apoptosis and angiogenesis in clear cell renal cell carcinoma tissues

  2008cited by this paper
• Reduced expression of CYLD in human colon and hepatocellular carcinomas.

  2007cited by this paper
• Treatment of gastric cancer.

  2006cited by this paper
• Updates on esophageal and gastric cancers.

  2006cited by this paper
• Nuclear factor-κB in cancer development and progression

  2006cited by this paper
• Treatment of gastric cancer.

  2006cited by this paper
• NF-kappaB in solid tumors.

  2006cited by this paper
• Cyld inhibits tumor cell proliferation by blocking Bcl-3-dependent NF-kappaB signaling.

  2006cited by this paper
• Nuclear factor-kappaB in cancer development and progression.

  2006cited by this paper
• Perioperative chemotherapy versus surgery alone for resectable gastroesophageal cancer.

  2006cited by this paper
• NF-κB in solid tumors

  2006cited by this paper
• Faculty Opinions recommendation of Cyld inhibits tumor cell proliferation by blocking Bcl-3-dependent NF-kappaB signaling.

  2006cited by this paper
• Helicobacter pylori Induces Plasminogen Activator Inhibitor 2 in Gastric Epithelial Cells through Nuclear Factor-κB and RhoA

  2004cited by this paper
• NF-kappaB is essential for induction of CYLD, the negative regulator of NF-kappaB: evidence for a novel inducible autoregulatory feedback pathway.

  2004cited by this paper
• Inducible nuclear factor-κB activation contributes to chemotherapy resistance in gastric cancer1 ☆

  2004cited by this paper
• NF-κB Is Essential for Induction of CYLD, the Negative Regulator of NF-κB

  2004cited by this paper
• Inducible nuclear factor-kappaB activation contributes to chemotherapy resistance in gastric cancer.

  2004cited by this paper
• CYLD is a deubiquitinating enzyme that negatively regulates NF-κB activation by TNFR family members

  2003cited by this paper
• Loss of the cylindromatosis tumour suppressor inhibits apoptosis by activating NF-κB

  2003cited by this paper
• Faculty Opinions recommendation of Loss of the cylindromatosis tumour suppressor inhibits apoptosis by activating NF-kappaB.

  2003cited by this paper
• Faculty Opinions recommendation of CYLD is a deubiquitinating enzyme that negatively regulates NF-kappaB activation by TNFR family members.

  2003cited by this paper
• The tumour suppressor CYLD negatively regulates NF-κB signalling by deubiquitination

  2003cited by this paper
• The epidemiology of gastric cancer

  2002cited by this paper
• Gastric cancer--patterns of relapse after surgical resection.

  2002cited by this paper
• Nuclear factor-kappaB p65 (RelA) transcription factor is constitutively activated in human gastric carcinoma tissue.

  2001cited by this paper
• Control of oncogenesis and cancer therapy resistance by the transcription factor NF-kappaB.

  2001cited by this paper
• Therapeutic potential of inhibition of the NF-kappaB pathway in the treatment of inflammation and cancer.

  2001cited by this paper
• Control of oncogenesis and cancer therapy resistance by the transcription factor NF-κB

  2001cited by this paper
• A method for establishing primary cultures of human gastric epithelial cells.

  2000cited by this paper
• A method for estabilishing primary cultures of human gastric epithelial cells

  2000cited by this paper
• Induction of apoptosis by cancer chemotherapy.

  2000cited by this paper
• [Treatment of gastric cancer].

  1999cited by this paper
• DNA damaging agents induce expression of Fas ligand and subsequent apoptosis in T lymphocytes via the activation of NF-kappa B and AP-1.

  1998cited by this paper
• Chemotherapy-induced apoptosis.

  1997cited by this paper
• Apoptosis and the dilemma of cancer chemotherapy.

  1997cited by this paper
• TNF- and cancer therapy-induced apoptosis: potentiation by inhibition of NF-kappaB.

  1996cited by this paper
• TNF- and Cancer Therapy-Induced Apoptosis: Potentiation by Inhibition of NF-κB

  1996cited by this paper
• [Treatment of gastric cancer].

  1982cited by this paper
• THE CANCER CELL

  1924cited by this paper

• High Frequency Loss of 17q11.2 and Downregulation of the Cancer Metastasis Suppression microRNA miR-193a-3p in Prostate Cancer Bone Metastasis

  2026cites this paper
• The emerging role of miR-362 in cancer: expression and function across different cancer types

  2025influential citation
• Cancer-associated fibroblast miR-148a-5p/CALD1/collagen VI pathway promotes proliferation in Helicobacter pylori-positive gastric cancer

  2025cites this paper
• Three possible diagnostic biomarkers for gastric cancer: miR-362-3p, miR-362-5p and miR-363-5p.

  2024cites this paper
• Ubiquitin-specific proteases: From biological functions to potential therapeutic applications in gastric cancer.

  2024cites this paper
• MiR362-3p Alleviates Osteosarcoma by Regulating the IL6ST/JAK2/STAT3 Pathway in Vivo and in Vitro

  2024cites this paper
• Novel therapeutic regimens for urethane-induced lung cancer in rats: II cisplatin nanoparticles combined with curcumin nanoparticls adjuvant

  2023cites this paper
• Mechanism of cisplatin resistance in gastric cancer and associated microRNAs

  2023cites this paper
• ER Negative Breast Cancer and miRNA: There Is More to Decipher Than What the Pathologist Can See!

  2023cites this paper
• Research Progress for Targeting Deubiquitinases in Gastric Cancers

  2022cites this paper
• Potential of natural products in the treatment of myocardial infarction: focus on molecular mechanisms

  2022cites this paper
• Contribution of MicroRNAs in Chemoresistance to Cisplatin in the Top Five Deadliest Cancer: An Updated Review

  2022cites this paper
• Involvement of Caspase-3 Pathway in Anti-cancer Properties of Genistein in AGS Gastric Cancer Cell Line is Still Enigmatic

  2022cites this paper
• Cascades between miRNAs, lncRNAs and the NF-κB signaling pathway in gastric cancer (Review)

  2021cites this paper
• MicroRNAs as the critical regulators of cisplatin resistance in gastric tumor cells

  2021influential citation
• NF-κB in Gastric Cancer Development and Therapy

  2021cites this paper
• Inhibitory feedback control of NF-κB signalling in health and disease

  2021cites this paper
• Mechanisms of Action And Clinical Implications of MicroRNAs in the Drug Resistance of Gastric Cancer

  2021cites this paper
• MicroRNA-362 negatively and positively regulates SMAD4 expression in TGF-β/SMAD signaling to suppress cell migration and invasion

  2021cites this paper
• miR-874-3p mitigates cisplatin resistance through modulating NF-κB/inhibitor of apoptosis protein signaling pathway in epithelial ovarian cancer cells

  2021cites this paper
• Current Status of MicroRNA-Based Biomarkers for Gastric Cancer

  2020cites this paper
• MiR-186 promotes the apoptosis of glioma U87 cells by down-regulating the expression of Smad6.

  2020cites this paper
• Recent Advancements in Biomarkers and Early Detection of Gastrointestinal Cancers

  2020cites this paper
• Integrated Analysis of a Noncoding RNA-Associated Competing Endogenous RNA Network in Non-Alcoholic Fatty Liver Disease

  2020cites this paper
• A MicroRNA Signature in Acute Coronary Syndrome Patients and Modulation by Colchicine

  2020cites this paper
• Comparison of microRNA Expression Profile in Chronic Myeloid Leukemia Patients Newly Diagnosed and Treated by Allogeneic Hematopoietic Stem Cell Transplantation

  2020cites this paper
• MiR-362-3p is downregulated by promoter methylation and independently predicts shorter OS of cervical squamous cell carcinoma.

  2019cites this paper
• Integrated analysis of miRNA and mRNA expression profiles in 2-, 6-, and 12-month-old Small Tail Han Sheep ovaries reveals that oar-miR-432 downregulates RPS6KA1 expression.

  2019cites this paper
• An overview of the multifaceted roles of miRNAs in gastric cancer: Spotlight on novel biomarkers and therapeutic targets

  2019cites this paper
• The effect of Helicobacter pylori on the expression of FRA-1 in gastric epithelial cells and its mechanism.

  2019cites this paper
• MicroRNA 495 Inhibits Proliferation and Metastasis and Promotes Apoptosis by Targeting Twist1 in Gastric Cancer Cells

  2019cites this paper
• Knockdown of microRNA-130b improves doxorubicin sensitivity in bladder urothelial carcinoma by negatively regulating cylindromatosis expression

  2019cites this paper
• Comparing MicroRNA Profilings of Purified HER-2-Negative and HER-2-Positive Cells Validates miR-362-5p/Sema3A as Characteristic Molecular Change in Triple-Negative Breast Cancers

  2019cites this paper
• miR-19a Is Involved In Progression And Malignancy Of Anaplastic Thyroid Cancer Cells

  2019cites this paper
• The role of deubiquitinating enzymes in gastric cancer

  2019cites this paper
• Myocardial hypertrophy is improved with berberine treatment via long non‐coding RNA MIAT‐mediated autophagy

  2019cites this paper
• Knockdown of microRNA-130b improves doxorubicin sensitivity in bladder urothelial carcinoma by negatively regulating cylindromatosis expression

  2019cites this paper
• Long non-coding RNA MEG-3 suppresses gastric carcinoma cell growth, invasion and migration via EMT regulation

  2019cites this paper
• Circulating MicroRNAs in Small-bowel Neuroendocrine Tumors: A Potential Tool for Diagnosis and Assessment of Effectiveness of Surgical Resection.

  2019cites this paper
• The emerging links between chromosomal instability (CIN), metastasis, inflammation and tumour immunity

  2019cites this paper
• MicroRNA-362 Inhibits Cell Proliferation and Invasion by Directly Targeting SIX1 in Colorectal Cancer

  2019cites this paper
• Let-7b attenuates cisplatin resistance and tumor growth in gastric cancer by targeting AURKB

  2018cites this paper
• MiR-362-5p as a novel prognostic predictor of cytogenetically normal acute myeloid leukemia

  2018cites this paper
• The upregulation of TMPRSS4, partly ascribed to the downregulation of miR-125a-5p, promotes the growth of human lung adenocarcinoma via the NF-κB signaling pathway

  2018cites this paper
• MiR-767 promoted cell proliferation in human melanoma by suppressing CYLD expression.

  2018cites this paper
• MiR-362-3p functions as a tumor suppressor through targeting MCM5 in cervical adenocarcinoma

  2018cites this paper
• TRIM44, a crucial target of miR-410, functions as a potential oncogene in osteosarcoma

  2018cites this paper
• Aberrant Expression of miR-362 Promotes Lung Cancer Metastasis through Downregulation of Sema3A

  2018cites this paper
• CHSY1 promoted proliferation and suppressed apoptosis in colorectal cancer through regulation of the NFκB and/or caspase-3/7 signaling pathway.

  2018cites this paper
• CircRNA circPDSS1 promotes the gastric cancer progression by sponging miR‐186‐5p and modulating NEK2

  2018cites this paper
• miR‑381 and miR‑489 suppress cell proliferation and invasion by targeting CUL4B via the Wnt/β‑catenin pathway in gastric cancer.

  2018cites this paper
• MiR-19a Overexpression in FTC-133 Cell Line Induces a More De-Differentiated and Aggressive Phenotype

  2018cites this paper
• Non-Coding RNAs in Cutaneous Melanoma Development, Progression and Dissemination

  2018cites this paper
• eration, migration and invasion by inactivating the Wnt/β-catenin pathway. miR-381/miR-489 overexpression recapitulated the effects of CUL4B silencing, while CUL4B restoration negated the suppressive effects induced by the ectopic expression of miR-381/miR-489. Furthermore, miR-381/miR-489 exerted t

  2018cites this paper
• Identifying heterogeneous subtypes of gastric cancer and subtype-specific subpaths of microRNA-target pathways

  2017cites this paper
• Long non-coding RNA DGCR5 is involved in the regulation of proliferation, migration and invasion of lung cancer by targeting miR-1180.

  2017cites this paper
• In vitro Evidence on Associations between MicroRNAs and Response to Therapy in Gastric Cancer: Report from the Encyclopedia Amlashica Systematic Reviews

  2017cites this paper
• NF-κB Signaling in Gastric Cancer

  2017cites this paper
• MicroRNA‐9 inhibits the gastric cancer cell proliferation by targeting TNFAIP8

  2017cites this paper
• Evaluation of miR-362 Expression in Astrocytoma of Human Brain Tumors

  2017cites this paper
• MicroRNA-362 is downregulated in cervical cancer and inhibits cell proliferation, migration and invasion by directly targeting SIX1.

  2017cites this paper
• Regulation of TRIM24 by miR-511 modulates cell proliferation in gastric cancer

  2017cites this paper
• Transcriptional and post-transcriptional regulation of Nrf2 in the heart by the deubiquitinase CYLD

  2016cites this paper
• Hsa-miRNA-31 regulates epithelial cell barrier function by inhibiting TNFSF15 expression.

  2016cites this paper
• Blood and lung microRNAs as biomarkers of pulmonary tumorigenesis in cigarette smoke-exposed mice

  2016cites this paper
• MicroRNA-223 Promotes Tumor Progression in Lung Cancer A549 Cells via Activation of the NF-κB Signaling Pathway

  2016cites this paper
• Noncoding RNAs Regulating NF-κB Signaling.

  2016cites this paper
• MiR-99a and MiR-491 Regulate Cisplatin Resistance in Human Gastric Cancer Cells by Targeting CAPNS1

  2016cites this paper
• miR-508-3p concordantly silences NFKB1 and RELA to inactivate canonical NF-κB signaling in gastric carcinogenesis

  2016cites this paper
• Interactions between anticancer active platinum complexes and non-coding RNAs/microRNAs

  2016influential citation
• miR-20a induces cisplatin resistance of a human gastric cancer cell line via targeting CYLD.

  2016cites this paper
• Mechanisms of Resistance to Chemotherapy in Gastric Cancer.

  2016cites this paper
• Downregulation of miR-362-5p inhibits proliferation, migration and invasion of human breast cancer MCF7 cells.

  2016cites this paper
• MicroRNAs: A Link Between Type 1 Diabetes and the Environment?

  2016cites this paper
• Significance of Tissue Expression and Serum Levels of Angiopoietin-like Protein 4 in Breast Cancer Progression: Link to NF-κB /P65 Activity and Pro-Inflammatory Cytokines.

  2016cites this paper
• Cullin 4A (CUL4A), a direct target of miR-9 and miR-137, promotes gastric cancer proliferation and invasion by regulating the Hippo signaling pathway

  2016cites this paper
• Resveratrol and acetyl-resveratrol modulate activity of VEGF and IL-8 in ovarian cancer cell aggregates via attenuation of the NF-κB protein

  2016cites this paper
• A combinative analysis of gene expression profiles and microRNA expression profiles identifies critical genes and microRNAs in oral lichen planus.

  2016cites this paper
• MicroRNA-506 inhibits gastric cancer proliferation and invasion by directly targeting Yap1

  2015cites this paper
• Non-coding RNAs in gastric cancer.

  2015cites this paper
• Upregulation of miR‐362‐3p Modulates Proliferation and Anchorage‐Independent Growth by Directly Targeting Tob2 in Hepatocellular Carcinoma

  2015cites this paper
• MiR-19a promotes epithelial-mesenchymal transition through PI3K/AKT pathway in gastric cancer.

  2015cites this paper
• Evaluation of miR-29c, miR-124, miR-135a and miR-148a in predicting lymph node metastasis and tumor stage of gastric cancer.

  2015cites this paper
• MicroRNAs May Serve as Emerging Molecular Biomarkers for Diagnosis and Prognostic Assessment or as Targets for Therapy in Gastric Cancer.

  2015cites this paper
• Anti-miR-362-3p Inhibits Migration and Invasion of Human Gastric Cancer Cells by Its Target CD82

  2015cites this paper
• miR-20a enhances cisplatin resistance of human gastric cancer cell line by targeting NFKBIB

  2015cites this paper
• MiR-454 prompts cell proliferation of human colorectal cancer cells by repressing CYLD expression.

  2015cites this paper
• Dysregulation of non-coding RNAs in gastric cancer.

  2015cites this paper
• IL-4 Up-Regulates MiR-21 and the MiRNAs Hosted in the CLCN5 Gene in Chronic Lymphocytic Leukemia

  2015cites this paper
• Brain death and marginal grafts in liver transplantation

  2015cites this paper
• Coxiella burnetii and Leishmania mexicana residing within similar parasitophorous vacuoles elicit disparate host responses

  2015cites this paper
• A panel of five serum miRNAs as a potential diagnostic tool for early-stage renal cell carcinoma

  2015cites this paper
• The functional sites of miRNAs and lncRNAs in gastric carcinogenesis

  2015cites this paper
• MicroRNA: master controllers of intracellular signaling pathways

  2015cites this paper
• CYLD-mediated signaling and diseases.

  2015cites this paper
• miR‐362‐5p inhibits proliferation and migration of neuroblastoma cells by targeting phosphatidylinositol 3‐kinase‐C2β

  2015cites this paper
• Dysregulation of non-coding RNAs in gastric cancer 2015 Advances in Gastric Cancer

  2015cites this paper
• A New Paradigm to Mitigate Osteosarcoma by Regulation of MicroRNAs and Suppression of the NF-κB Signaling Cascade.

  2014cites this paper
• Differential expression of microRNA501-5p affects the aggressiveness of clear cell renal carcinoma

  2014cites this paper
• Activity and expression pattern of NF-κB /P65 in peripheral blood from hepatocellular carcinoma patients - link to hypoxia inducible factor -1α.

  2014cites this paper