Topors acts as a SUMO‐1 E3 ligase for p53 in vitro and in vivo

Human Topors, which was originally identified as cellular binding partner of DNA topoisomerase I and of p53, has recently been shown to function as an ubiquitin E3 ligase for p53 in a manner dependent on its N′‐terminally located RING finger. Here, we demonstrate that Topors also enhances the conjugation of the small ubiquitin‐like modifier 1 (SUMO‐1) to p53 in vivo and in a reconstituted in vitro system. The Topors SUMO‐1 E3 ligase activity does not depend upon its RING finger motif. In HeLa cells, Topors induced p53 sumoylation was accompanied by an increase in endogenous p53 protein levels. Furthermore, Topors enhances the sumoylation of a variety of other, yet unidentified, cellular proteins.

• Publication year

  2005

• Venue

  FEBS Letters

• Publication date

  2005-09-12

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1016/j.febslet.2005.07.088PMID 16122737
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

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