The kinetic properties of yeast and muscle phosphoglyceric acid mutase.

During the past quarter of a century, the enzymes of the glycolytic scheme, with the exception of phosphoglyceric acid mutase, have been highly purified, and the properties of many of them have been investigated in detail. The first description of phosphoglyceric acid mutase by Meyerhof and Kiessling (1) arose from their discovery that phosphoglyceric acid isolated from yeast consisted of a mixture of 2and 3-phosphoglyceric acids. Sutherland, Posternak, and Cori (2,3) showed that 2,3-diphosphoglyceric acid, first isolated 25 years earlier by Greenwald (4), exhibited coenzyme activity for this enzyme. They also reported a partial purification of the enzyme from extracts of rabbit skeletal muscle and pointed out that these preparations contained diphosphoglycerate phosphatase activity. More recently, Cowgill and Pizer (5) have also reported a partial purification of mutasel from the same source and described some of its properties. The crystallization of mutase from bakers’ yeast has recently been reported by us (6). This paper presents a procedure for the preparation of a highly purified mutase from rabbit skeletal muscle. Although not crystalline, this preparation has a specific activity comparable to that of the crystalline yeast enzyme. In addition, we present a comparative study of the enzymatic

• Publication year

  1957

• Venue

  Journal of Biological Chemistry

• Publication date

  1957-10-01

• Fields of study

  Biology, Medicine, Chemistry

• Identifiers
  DOI 10.1016/s0021-9258(18)70667-4PMID 13475367
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• The rabbit skeletal muscle preparation is not crystalline but has a specific activity comparable to that of the crystalline yeast enzyme.

  Confidence 0.94

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) reviewGahyoun (29ua5897xm) review
• A procedure is presented for preparing a highly purified phosphoglyceric acid mutase from rabbit skeletal muscle.

  Confidence 0.98

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) reviewGahyoun (29ua5897xm) review

• crystalline yeast enzyme

  reference preparation

  The crystalline phosphoglyceric acid mutase preparation from baker's yeast used for comparison.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) reviewGahyoun (29ua5897xm) review
• highly purified mutase preparation

  biochemical preparation

  The muscle enzyme preparation obtained by the purification procedure described in the abstract.

  Aliases: purified mutase

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) reviewGahyoun (29ua5897xm) review
• phosphoglyceric acid mutase

  enzyme

  An enzyme of the glycolytic pathway that interconverts 2- and 3-phosphoglyceric acid forms.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) reviewGahyoun (29ua5897xm) review
• purification procedure

  method

  The method used to isolate and purify phosphoglyceric acid mutase from rabbit skeletal muscle.

  Aliases: procedure for the preparation

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) reviewGahyoun (29ua5897xm) review
• rabbit skeletal muscle

  source tissue

  Skeletal muscle tissue from rabbit used as the enzyme source in the preparation.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) reviewGahyoun (29ua5897xm) review
• specific activity

  measure

  The enzyme activity normalized by amount of material, used here to compare the muscle and yeast preparations.

  박진우 (dztg5apj7m) extractionB (s683577b42) reviewAnonymous (12632b8b5f) reviewGahyoun (29ua5897xm) review

• The microestimation, distribution, and biosynthesis of 2, 3-diphosphoglyceric acid.

  1957cited by this paper
• The molecular properties of yeast and muscle phosphoglyceric acid mutase.

  1957cited by this paper
• Purification and properties of fructose-1, 6-diphosphatase.

  1956cited by this paper
• Crystalline phosphoglyceric acid mutase.

  1956cited by this paper
• The occurrence of a group transfer involving enzyme (phosphoglucomutase) and substrate.

  1954cited by this paper
• The interaction of DL-2-phosphoglyceric acid with metal ions activating enolase.

  1954cited by this paper
• The interaction of purified enolase with its activating metal-ions.

  1953cited by this paper
• A synthesis of 2,3-diphospho-D-glyceric acid.

  1950cited by this paper
• Mechanism of the phosphoglyceric mutase reaction.

  1949cited by this paper
• Manometric techniques and tissue metabolism

  1949cited by this paper
• The enzymatic equilibria of phospho(enol)pyruvate.

  1949cited by this paper
• The mechanism of action of phosphoglucomutase and phosphoglyceric acid mutase.

  1949cited by this paper

• Revisited Metabolic Control and Reprogramming Cancers by Means of the Warburg Effect in Tumor Cells

  2022cites this paper
• Characterization of genetically modified mice for phosphoglycerate mutase, a vitally-essential enzyme in glycolysis

  2021cites this paper
• Phosphoglycerate Mutase Cooperates with Chk1 Kinase to Regulate Glycolysis

  2020cites this paper
• The role of phosphoglycerate mutase 1 in tumor aerobic glycolysis and its potential therapeutic implications

  2014cites this paper
• Method for the enzymatic synthesis of 2-phospho-D-glycerate from adenosine 5'-triphosphate and D-glycerate via D-glycerate-2-kinase

  2005cites this paper
• Pathway of glycogen metabolism in Methanococcus maripaludis

  1994cites this paper
• Pathway ofGlycogen Metabolism inMethanococcus maripaludis

  1994cites this paper
• 1H and 51V NMR studies of the interaction of vanadate and 2-vanadio-3-phosphoglycerate with phosphoglycerate mutase.

  1992cites this paper
• Stimulation of human erythrocyte 2,3-bisphosphoglycerate phosphatase by vanadate.

  1990cites this paper
• The phosphoglycerate mutases.

  1989cites this paper
• The catalytic bimodality of mammalian phosphoglycerate mutase.

  1986cites this paper
• Wheat germ phosphoglycerate mutase: evidence for a metalloenzyme.

  1986cites this paper
• Purification and properties of the phosphoglycerate mutase isozymes from the mouse.

  1985cites this paper
• The veillonellae: gram-negative cocci with a unique physiology.

  1985cites this paper
• Wheat germ phosphoglycerate mutase: purification, polymorphism, and inhibition.

  1985cites this paper
• Purification and characterization of phosphopentomutase from rat liver.

  1983cites this paper
• The complete amino acid sequence of human erythrocyte diphosphoglycerate mutase.

  1983cites this paper
• Purification, characterization and inhibition by MK-401 of Fasciola hepatica phosphoglyceromutase.

  1982cites this paper
• The enzymology of 2,3-bisphosphoglycerate.

  1980cites this paper
• Coordination of protein synthesis and degradation.

  1979cites this paper
• A comparison of the association of yeast phosphoglycerate mutase (EC2.7.5.3) with that of haemoglobin. An ultracentrifuge study.

  1977cites this paper
• Phosphoglycerate mutase from wheat germ: studies with isotopically labeled 3-phospho-D-glycerates showing that the catalyzed reaction is intramolecular. Appendix: phosphoglycerate mutase from wheat germ: isolation, crystallization, and properties.

  1977cites this paper
• Purification and Characterization of Phosphoglycerate Mutase from Methanol-grown Hyphomicrobium x and Pseudomonas

  1976cites this paper
• Purification and characterization of phosphoglycerate mutase from methanol-grown Hyphomicrobium X and Pseudomonas AM1.

  1976cites this paper
• Kinetic competence of a phosphoryl enzyme intermediate in the glucose-1,6-p2 synthase-catalyzed reaction. Purification, properties, and kinetic studies.

  1976cites this paper
• Phosphoglycerate mutase from yeast, chicken breast muscle, and kidney (2, 3-PGA-dependent).

  1975cites this paper
• Purification of bisphosphoglyceromutase, 2,3-bisphosphoglycerate phosphatase and phosphoglyceromutase from human erthrocytes. Three enzyme activities in one protein.

  1975cites this paper
• The amino acid sequence of yeast phosphoglycerate mutase

  1975cites this paper
• Cell wall composition and incorporation of radio-labelled compounds by Veillonella alcalescens.

  1975cites this paper
• The sequence of a peptide containing the active site phosphohistidine residue of phosphoglycerate mutase from chicken breast muscle.

  1975cites this paper
• Purification of Bisphosphoglyceromutase, 2, 3‐Bisphosphoglycerate Phosphatase and Phosphoglyceromutase from Human Erythrocytes

  1975cites this paper
• The mechanism of phosphoglucomutase from Micrococcus lysodeikticus.

  1974cites this paper
• The equilibrium constant of the phosphoglyceromutase reaction.

  1974cites this paper
• [Familial diphosphoglycerate mutase deficiency: hematological and biochemical study].

  1972cites this paper
• Multiple forms of phosphoglyceromutase from chicken breast muscle.

  1972cites this paper
• Formation of an active phosphoenzyme by diphosphoglycerate-dependent phosphoglyceromutases from muscle, kidney and yeast.

  1972cites this paper
• Mechanism of the 2,3-diphosphoglycerate-dependent phosphoglycerate mutase from rabbit muscle.

  1972cites this paper
• Identification and characterization of pituitary triose-phosphate isomerase.

  1972cites this paper
• Purification and properties of phosphoglyceromutase from sheep muscle.

  1971cites this paper
• Mechanism of action of 2,3-diphosphoglycerate-independent phosphoglycerate mutase.

  1971cites this paper
• Die hämolytischen Anämien

  1970cites this paper
• The effects of mercury and other reagents on phosphoglycerate mutase-2,3-diphosphoglycerate phosphatase from kidney, muscle and other tissues.

  1970cites this paper
• The 2,3-diphosphoglyceric acid phosphatase activity of phosphoglyceric acid mutase purified from human erythrocytes.

  1970cites this paper
• Glycolysis: Regulation and Mechanisms of the Enzymes

  1969cites this paper
• Lactate Metabolism in Fish

  1969cites this paper
• Purification and properties of 3-phosphoglyceric acid mutase from caprine erythrocytes.

  1969cites this paper
• Phosphoglyceric acid mutase. A computer simulation study.

  1969cites this paper
• A comparative study on the phosphoglyceric acid cycle in mammalian erythrocytes.

  1969cites this paper
• Influence of salt, substrate, and cofactor concentrations on the kinetic and mechanistic behavior of phosphoglycerate mutase.

  1968cites this paper
• Mercury-induced reversible increase in 2,3-diphosphoglycerate phosphatase and concomitant decrease in mutase activity of animal phosphoglycerate mutases.

  1967cites this paper
• Influence of ionic strength on apparent reaction mechanism of phosphoglycerate mutase.

  1966cites this paper
• The purification and properties of phosphoglycerate mutase from chicken breast muscle.

  1966cites this paper
• The phosphorylated intermediate in the phosphoglyceromutase reaction.

  1966cites this paper
• Phosphoryl intermediates formed with phosphoglycerate mutase. Role and labilization of 2,3-diphosphoglycerate.

  1966cites this paper
• The phosphoglucomutase, phosphoglyceric acid mutase, and phosphomannose isomerase activities of arterial tissue in individuals of various ages.

  1966cites this paper
• Purification and properties of 3-beta-hydroxysteroid dehydrogenase and delta-5-3-ketosteroid isomerase from bovine corpora lutea.

  1966cites this paper
• Immunological studies on glycerate 2,3 diphosphatase and phosphoglycerate mutase.

  1965cites this paper
• Phosphate transfer from adenosine diphosphate phosphoglycerate and 2,3-diphosphoglycerate.

  1965cites this paper
• STUDIES ON THE SYNTHESIS OF DEOXYRIBONUCLEIC ACID BY MAMMALIAN ENZYMES. I. INCORPORATION OF DEOXYRIBONUCLEOSIDE 5'-TRIPHOSPHATES INTO DEOXYRIBONUCLEIC ACID BY A PARTIALLY PURIFIED ENZYME FROM REGENERATING RAT LIVER.

  1964cites this paper
• EFFECT OF ISCHEMIA ON KNOWN SUBSTRATES AND COFACTORS OF THE GLYCOLYTIC PATHWAY IN BRAIN.

  1964cites this paper
• Glutamate dehydrogenase from yeast and from animal tissues

  1964cites this paper
• THE CATALYTIC ENVIRONMENT AND ITS BIOLOGICAL IMPLICATIONS.

  1964cites this paper
• THE RELATIONSHIPS BETWEEN SUBSTRATES AND ENZYMES OF GLYCOLYSIS IN BRAIN.

  1964cites this paper
• Studies on the Synthesis of Deoxyribonucleic Acid by Mammalian Enzymes II. AN INVESTIGATION OF THE PRIMER REQUIREMENTS OF PARTIALLY PURIFIED REGENERATING RAT LIVER DEOXYRIBONUCLEIC ACID NUCLEOTIDYLTRANSFERASE

  1964cites this paper
• THE INFLUENCE OF SULFHYDRYL GROUPS ON THE STABILITY OF CARBAMYLPHOSPHATE SYNTHETASE.

  1964cites this paper
• Enzymes of Glycolysis

  1964cites this paper
• ON THE NATURE OF THE PHOSPHOENZYME INTERMEDIATE IN THE PHOSPHOGLYCEROMUTASE REACTION.

  1963cites this paper
• Inactivation of muscle triosephosphate dehydrogenase by reduced diphosphopyridine nucleotide at physiological concentrations.

  1962cites this paper
• Studies of metabolic alterations induced by steroids in murine lymphocytic leukemia cells in vitro.

  1962cites this paper
• Permeability changes following infection of Escherichia coli with T2 bacteriophage, as measured by reduced diphosphopyridine nucleotide oxidase activity.

  1962cites this paper
• Glutamate-dehydrogenase inactivation by reduced nicotinamide-adenine dinucleotide phosphate.

  1962cites this paper
• The purification and properties of reduced diphosphopyridine nucleotide oxidase from uninfected and T2 infected Escherichia coli B.

  1962cites this paper
• [26] Phosphoglyceric Acid Mutase: 2,3-DPGA + 3-PGA ⇄ 2,3-DPGA + 2-PGA (crystalline) (1); 3-PGA ⇄ 2-PGA (2)

  1962cites this paper
• [27] Glycerate 2,3-diphosphatase: D-2,3-Diphosphoglycerate+H2O→Phosphoglycerate+Pi

  1962cites this paper
• Studies on the mechanism of action of phosphoglyceromutases.

  1961cites this paper
• The presence of adenylyl 2, 3-diphosphoglyceric acid in pig blood.

  1961cites this paper
• Phosphate esters produced from inosine in human erythrocyte ghosts.

  1961cites this paper
• Phosphoglyceric acid mutase activity without added 2,3-diphosphoglycerate in preparations purified from rice germ.

  1960cites this paper
• Properties of the phosphoprotein-phosphoglyceric mutase.

  1960cites this paper
• CHAPTER 1 – Free Energy and the Biosynthesis of Phosphates

  1960cites this paper
• Wege des Abbaues von Kohlenhydraten bei Sauerstoffatmung und Gärungen

  1960cites this paper
• Crystallized enzymes from the myogen of rabbit skeletal muscle.

  1960cites this paper
• Distribution of two types of phosphoglyceric acid mutase, diphosphoglycerate mutase, and D-2,3-diphosphoglyceric acid.

  1959cites this paper
• Phosphoglyceric acid mutase activity without added 2,3-diphosphoglycerate in preparations purified from wheat germ.

  1959cites this paper
• Studies on the biosynthesis of DNA by cell-free extracts of mouse leukemic cells.

  1959cites this paper
• The purification and properties of muscle diphosphoglycerate mutase.

  1959cites this paper
• ENERGY‐GENERATING SYSTEMS

  1959cites this paper
• Human red cell glycolytic intermediates.

  1959cites this paper
• Adenosine mono-, di- and triphosphate, pyruvic kinase, hexokinase and polynucleotide phosphorylase assay.

  1958cites this paper
• Studies on glycerate 2,3-diphosphatase.

  1958cites this paper
• Carbohydrate metabolism.

  1958cites this paper
• The molecular properties of yeast and muscle phosphoglyceric acid mutase.

  1957cites this paper