Toward an artificial cell based on gene expression in vesicles

No abstract is available for this paper.

• Publication year

  2005

• Venue

  Physical Biology

• Publication date

  2005-09-15

• Fields of study

  Biology, Physics, Computer Science, Engineering, Medicine

• Identifiers
  DOI 10.1088/1478-3975/2/3/P01PMID 16224117
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• NMR Structure of Mistic, a Membrane-Integrating Protein for Membrane Protein Expression

  2005cited by this paper
• Cell‐free Protein Synthesis through Solubilisate Exchange in Water/Oil Emulsion Compartments

  2004cited by this paper
• The Emergence of Competition Between Model Protocells

  2004cited by this paper
• Phospholipase A2 promotes raft budding and fission from giant liposomes.

  2004cited by this paper
• Determination of the Core of a Minimal Bacterial Gene Set

  2004cited by this paper
• A vesicle bioreactor as a step toward an artificial cell assembly.

  2004cited by this paper
• Engineering asymmetric vesicles

  2003cited by this paper
• Imaging coexisting fluid domains in biomembrane models coupling curvature and line tension

  2003cited by this paper
• Principles of cell-free genetic circuit assembly

  2003cited by this paper
• Tinker, Tailor: Can Venter Stitch Together a Genome From Scratch?

  2003cited by this paper
• Artificial cells: prospects for biotechnology.

  2002cited by this paper
• Cell-free translation reconstituted with purified components

  2001cited by this paper
• Unfolding and internalization of proteins by the ATP-dependent proteases ClpXP and ClpAP.

  2000influential reference
• A synthetic oscillatory network of transcriptional regulators

  2000cited by this paper
• Chemical transformations in individual ultrasmall biomimetic containers.

  1999cited by this paper
• Quantitative studies on the melittin-induced leakage mechanism of lipid vesicles.

  1998cited by this paper
• Man-made cell-like compartments for molecular evolution

  1998cited by this paper
• Effect of the ATP level on the overall protein biosynthesis rate in a wheat germ cell-free system.

  1996cited by this paper
• Structure of Staphylococcal α-Hemolysin, a Heptameric Transmembrane Pore

  1996cited by this paper
• The conformation of membranes

  1991cited by this paper
• A continuous cell-free translation system capable of producing polypeptides in high yield.

  1988cited by this paper
• Ff coliphages: structural and functional relationships.

  1986cited by this paper
• Bombolitins, a new class of mast cell degranulating peptides from the venom of the bumblebee Megabombus pennsylvanicus.

  1985cited by this paper
• Asymmetric orientation of phage M13 coat protein in Escherichia coli cytoplasmic membranes and in synthetic lipid vesicles.

  1976cited by this paper
• The energy charge of the adenylate pool as a regulatory parameter. Interaction with feedback modifiers.

  1968cited by this paper
• Theory Of Self Reproducing Automata

  1967cited by this paper

• 0.46 Terahertz wave irradiation inhibit transcription reaction in liposomes

  2025cites this paper
• Controlled Formation of DNA Condensates as Model Nuclei in Monodisperse Giant Vesicles

  2025cites this paper
• Microfluidics meets cell-free systems: from molecular engineering to synthetic cells.

  2025cites this paper
• RNA synthesis in liposomes with negatively charged lipids after fusion via freezing-thawing.

  2024cites this paper
• A comprehensive review of Microfluidic approaches in cell-free synthetic biology

  2024cites this paper
• Present and future of synthetic cell development

  2023cites this paper
• Synthetic Intracellular Environments: From Basic Science to Applications.

  2023cites this paper
• DNA droplets for intelligent and dynamical artificial cells: from the viewpoint of computation and non-equilibrium systems

  2023cites this paper
• Effects of Charged Lipids on Giant Unilamellar Vesicle Fusion and Inner Content Mixing via Freeze‐Thawing

  2022cites this paper
• Cell-free synthetic biology as an emerging biotechnology

  2022cites this paper
• A microfluidic optimal experimental design platform for forward design of cell-free genetic networks

  2022cites this paper
• Parasites, Infections, and Inoculation in Synthetic Minimal Cells

  2022cites this paper
• The fabrication of phospholipid vesicle-based artificial cells and their functions

  2021cites this paper
• Plug-and-Play Microfluidic Production of Monodisperse Giant Unilamellar Vesicles Using Droplet Transfer across Water–Oil Interface

  2021cites this paper
• Chemical-mediated translocation in protocell-based microactuators

  2021cites this paper
• Modularize and Unite: Toward Creating a Functional Artificial Cell

  2021cites this paper
• The all-E. coliTXTL toolbox 3.0: new capabilities of a cell-free synthetic biology platform

  2021cites this paper
• Controlled metabolic cascades for protein synthesis in an artificial cell.

  2021cites this paper
• Membrane functionalization in artificial cell engineering

  2020cites this paper
• Versatile Phospholipid Assemblies for Functional Synthetic Cells and Artificial Tissues

  2020cites this paper
• Reconstituted cell-free protein synthesis using in vitro transcribed tRNAs

  2020cites this paper
• Recent advancements in liposome technology.

  2020cites this paper
• Membrane functionalization in artificial cell engineering

  2020cites this paper
• Reconstituted cell-free protein synthesis using in vitro transcribed tRNAs

  2020cites this paper
• Catalytic processing in ruthenium-based polyoxometalate coacervate protocells

  2020cites this paper
• pH‐Gated Activation of Gene Transcription and Translation in Biocatalytic Metal–Organic Framework Artificial Cells

  2020cites this paper
• A Sustainable and Efficient Artificial Microgel System: Toward Creating a Configurable Synthetic Cell.

  2020cites this paper
• A MATLAB toolbox for modeling genetic circuits in cell-free systems†

  2020cites this paper
• Mechanical characterization of vesicles and cells: A review

  2020cites this paper
• Compartmentalizing Cell-Free Systems: Toward Creating Life-Like Artificial Cells and Beyond.

  2020cites this paper
• A bead-based method for the removal of the amino acid lysine from cell-free transcription-translation systems

  2020cites this paper
• Interface Engineering in Multiphase Systems toward Synthetic Cells and Organelles: From Soft Matter Fundamentals to Biomedical Applications

  2020cites this paper
• Controlling Secretion in Artificial Cells with a Membrane AND Gate.

  2019cites this paper
• An E. coli cell-free transcription- translation system: modeling gene expression and characterizing CRISPR elements and gene circuits

  2019cites this paper
• Artificial morphogen-mediated differentiation in synthetic protocells

  2019cites this paper
• Synthetic Biology Goes Cell-Free

  2019cites this paper
• Minimalist Protocell Design: A Molecular System Based Solely on Proteins that Form Dynamic Vesicular Membranes Embedding Enzymatic Functions

  2019cites this paper
• Microfluidics for Artificial Life: Techniques for Bottom-Up Synthetic Biology

  2019cites this paper
• Geometric Effect for Biological Reactors and Biological Fluids

  2018cites this paper
• Emerging Biomimetic Applications of DNA Nanotechnology.

  2018cites this paper
• Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system

  2018cites this paper
• Engineering approaches of smart, bio-inspired vesicles for biomedical applications

  2018cites this paper
• Engineering thermoresponsive phase separated vesicles formed via emulsion phase transfer as a content-release platform

  2018cites this paper
• Bacterial cell-free expression technology to in vitro systems engineering and optimization

  2017cites this paper
• Gene-Mediated Chemical Communication in Synthetic Protocell Communities.

  2017cites this paper
• Multi-dimensional studies of synthetic genetic promoters enabled by microfluidic impact printing.

  2017cites this paper
• Artificial cell mimics as simplified models for the study of cell biology

  2017cites this paper
• Multi-compartment encapsulation of communicating droplets and droplet networks in hydrogel as a model for artificial cells

  2017cites this paper
• Synthetic membranes in microfluidic interfaces

  2017cites this paper
• Cell-free protein synthesis in micro compartments: building a minimal cell from biobricks.

  2017cites this paper
• On-chip density-based purification of liposomes.

  2017cites this paper
• Pulse-density modulation control of chemical oscillation far from equilibrium in a droplet open-reactor system

  2016cites this paper
• The All E. coli TX-TL Toolbox 2.0: A Platform for Cell-Free Synthetic Biology.

  2016cites this paper
• Development of microfluidic platforms to construct giant unilamellar vesicles (GUVs) for the biophysical study of lipid membranes

  2016cites this paper
• Utilization of Bioorthogonal Chemistry for Live-Cell Labeling of Glycoconjugates and the Liposomal-Based Engineering of Dynamic Artificial Cellular Models

  2016cites this paper
• DNA Microgels as a Platform for Cell-Free Protein Expression and Display.

  2016cites this paper
• In vitro membrane protein synthesis inside Sec translocon-reconstituted cell-sized liposomes

  2016cites this paper
• Characterizing redundant rigidity and redundant global rigidity of body-hinge graphs

  2016cites this paper
• The relationship between genotype and phenotype in cell-free transcription-translation reactions.

  2016cites this paper
• Polynucleotides in cellular mimics: Coacervates and lipid vesicles

  2016cites this paper
• Bioinspired nanoreactors for the biomineralisation of metallic-based nanoparticles for nanomedicine.

  2015cites this paper
• Chemical evolution and life

  2015cites this paper
• Towards Autonomous Control of Molecular Communication in Populations of Bacteria

  2015cites this paper
• Synthetic Biology: Computational Modeling Bridging the Gap between In Vitro and In Vivo Reactions

  2015cites this paper
• Engineering artificial cells by combining HeLa-based cell-free expression and ultrathin double emulsion template.

  2015cites this paper
• Autonomous buckling of micrometer-sized lipid-protein membrane patches constructed by Dictyostelium discoideum

  2015cites this paper
• Microfluidic Tools and Techniques for Artificial Cell Engineering

  2015influential citation
• Synthetic Biology: A Bridge between Artificial and Natural Cells

  2014cites this paper
• In vitro membrane protein synthesis inside cell-sized vesicles reveals the dependence of membrane protein integration on vesicle volume.

  2014cites this paper
• Application of nucleic acid-lipid conjugates for the programmable organisation of liposomal modules.

  2014cites this paper
• Incorporation de protéines membranaires produites par un système d'expression protéique acellulaire dans des bicouches lipidiques planes

  2014cites this paper
• Membrane protein synthesis in cell‐free systems: From bio‐mimetic systems to bio‐membranes

  2014cites this paper
• On the Symmetric Molecular Conjectures

  2014cites this paper
• Synthetic Biology Outside the Cell: Linking Computational Tools to Cell-Free Systems

  2014cites this paper
• Toward the assembly of a minimal divisome

  2014cites this paper
• Engineering DNA Gels For Cell-Free Protein Production

  2014cites this paper
• DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation.

  2014cites this paper
• Cell-free expression of GPCRs: the endothelin system

  2014cites this paper
• The engineering of artificial cellular nanosystems using synthetic biology approaches.

  2014cites this paper
• Synthetic Biology and Synthetic Knowledge

  2013cites this paper
• Protein Binding Interfaces and Their Binding Hot Spot Prediction: A Survey

  2013cites this paper
• Extension of the Two Band Model

  2013cites this paper
• The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics

  2013cites this paper
• Microfluidic methods for forming liposomes.

  2013cites this paper
• Darwinian evolution in a translation-coupled RNA replication system within a cell-like compartment

  2013cites this paper
• Microbial diversity and the “lower-limit” problem of biodiversity

  2013cites this paper
• An engineered dimeric protein pore that spans adjacent lipid bilayers

  2013cites this paper
• Microbial diversity and the “lower-limit” problem of biodiversity

  2013cites this paper
• Modeling pre-biotic self-organization: The chemical dynamics of autocatalytic networks

  2013cites this paper
• As We May Be Doing Philosophy: Informationalism – A New Regime for Philosophy?

  2013cites this paper
• Ensemble Bayesian analysis of bistability in a synthetic transcriptional switch.

  2012cites this paper
• Synthetic biology, inspired by synthetic chemistry

  2012cites this paper
• Triggered gene expression in fed-vesicle microreactors with a multifunctional membrane.

  2012cites this paper
• Towards an artificial cell

  2012cites this paper
• Nonlinear Dynamical Systems as Models of Development of Inorganic Cells (iCHELLs) & their Simple Assemblies

  2012cites this paper
• Dynamics of pore synthesis and degradation in protocells

  2012cites this paper
• Fabricación de liposomas y de cápsulas poliméricas

  2012cites this paper
• AOT-Vesicles Produced at the Oil-Water Interface

  2011cites this paper
• Exploration, iterativity and kludging in synthetic biology

  2011cites this paper
• Cell-Free Protein Expression under Macromolecular Crowding Conditions

  2011cites this paper