DNA-functionalized hydrogels for confined membrane-free in vitro transcription/translation.

No abstract is available for this paper.

• Publication year

  2014

• Venue

  Lab on a Chip

• Publication date

  2014-07-03

• Fields of study

  Biology, Materials Science, Chemistry, Engineering, Medicine

• Identifiers
  DOI 10.1039/c3lc51427gPMID 24663810
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Biocompatible macro-initiators controlling radical retention in microfluidic on-chip photo-polymerization of water-in-oil emulsions.

  2014cited by this paper
• Enhanced transcription rates in membrane-free protocells formed by coacervation of cell lysate

  2013cited by this paper
• Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics.

  2013cited by this paper
• Stabilization and enhanced reactivity of actinorhodin polyketide synthase minimal complex in polymer-nucleotide coacervate droplets.

  2012cited by this paper
• Massively parallel single-molecule and single-cell emulsion reverse transcription polymerase chain reaction using agarose droplet microfluidics.

  2012cited by this paper
• Stochastic branching-diffusion models for gene expression

  2012cited by this paper
• Cell-Free Protein Expression from DNA-Based Hydrogel (P-Gel) Droplets for Scale-Up Production

  2012cited by this paper
• Experiment and mathematical modeling of gene expression dynamics in a cell-free system.

  2012cited by this paper
• A mechanical metamaterial made from a DNA hydrogel.

  2012cited by this paper
• Hydrogels with time-dependent material properties enhance cardiomyocyte differentiation in vitro.

  2011cited by this paper
• Facile synthesis and characterization of disulfide-cross-linked hyaluronic acid hydrogels for protein delivery and cell encapsulation.

  2011cited by this paper
• Hyaluronic Acid Hydrogels for Biomedical Applications

  2011cited by this paper
• DNA-templated assembly of droplet-derived PEG microtissues.

  2011cited by this paper
• BglBricks: A flexible standard for biological part assembly

  2010cited by this paper
• Spatial organization of the flow of genetic information in bacteria

  2010cited by this paper
• A biocompatible condensation reaction for controlled assembly of nanostructures in live cells

  2010cited by this paper
• The life of an mRNA in space and time

  2010cited by this paper
• Synthesis and characterization of hyaluronic acid-poly(ethylene glycol) hydrogels via Michael addition: An injectable biomaterial for cartilage repair.

  2010cited by this paper
• Reaction-diffusion systems in intracellular molecular transport and control.

  2010cited by this paper
• Thiol-ene click chemistry.

  2010cited by this paper
• Rapid prototyping of microstructures by soft lithography for biotechnology.

  2010cited by this paper
• An Integrated Device for Monitoring Time‐Dependent in vitro Expression From Single Genes in Picolitre Droplets

  2008cited by this paper
• Exporting RNA from the nucleus to the cytoplasm

  2007cited by this paper
• Regulation of translation via mRNA structure in prokaryotes and eukaryotes.

  2005cited by this paper
• Microfluidic biosensor based on an array of hydrogel-entrapped enzymes.

  2005cited by this paper
• Toward an artificial cell based on gene expression in vesicles

  2005cited by this paper
• tRNA dynamics on the ribosome during translation.

  2004cited by this paper
• The hydrodynamic radii of macromolecules and their effect on red blood cell aggregation.

  2004cited by this paper
• Photocrosslinked hyaluronic acid hydrogels: natural, biodegradable tissue engineering scaffolds.

  2003cited by this paper
• Disulfide cross-linked hyaluronan hydrogels.

  2002cited by this paper
• Synthesizing life

  2001cited by this paper
• Initiation of translation in prokaryotes and eukaryotes.

  1999cited by this paper
• Recent advances in producing and selecting functional proteins by using cell-free translation.

  1998cited by this paper
• A continuous cell-free translation system capable of producing polypeptides in high yield.

  1988cited by this paper
• Studies of ribosomal diffusion coefficients using laser light-scattering spectroscopy.

  1974cited by this paper
• Tissue sulfhydryl groups.

  1959cited by this paper

• Transcript Identification Using Arrayed Hydrogels With TrapFISH

  2025cites this paper
• Regulating Protein Immobilization During Cell‐Free Protein Synthesis in Hyaluronan Microgels

  2025cites this paper
• Cell-Free Protein Synthesis of Fusion-Protein Building Blocks Enables Autonomous Growth in Globular Protein Vesicle Protocells.

  2025cites this paper
• DNA-based hydrogels: Ideal biomaterials for cartilage organoids

  2024cites this paper
• From Single‐Compartment Artificial Cells to Tissue‐Like Materials

  2024cites this paper
• Lab on a Particle Technologies

  2024cites this paper
• Hydrogel-Encapsulated Beads Enable Proximity-Driven Encoded Library Synthesis and Screening

  2023cites this paper
• In Vitro Transcription–Translation in an Artificial Biomolecular Condensate

  2023cites this paper
• Compartmentalized Cell-Free Expression Systems for Building Synthetic Cells.

  2023cites this paper
• Bottom-Up Synthetic Biology Using Cell-Free Protein Synthesis.

  2023cites this paper
• Hydrogels as functional components in artificial cell systems

  2022cites this paper
• Processing of fast-gelling hydrogel precursors in microfluidics by electrocoalescence of reactive species

  2021cites this paper
• Artificial Organelles

  2021cites this paper
• Hydrogels for 3D Cell Culture

  2021cites this paper
• Programming Diffusion and Localization of DNA Signals in 3D-Printed DNA-Functionalized Hydrogels.

  2020cites this paper
• Translation-coupled RNA replication and parasitic replicators in membrane-free compartments.

  2020cites this paper
• Microfluidic Fabrication of Click Chemistry-Mediated Hyaluronic Acid Microgels: A Bottom-Up Material Guide to Tailor a Microgel’s Physicochemical and Mechanical Properties

  2020cites this paper
• Bottom-Up Construction of Complex Biomolecular Systems With Cell-Free Synthetic Biology

  2020cites this paper
• Lipid sponge droplets as programmable synthetic organelles

  2020cites this paper
• Cell-free protein synthesis and in situ immobilization of deGFP-MatB in polymer microgels for malonate-to-malonyl CoA conversion

  2020cites this paper
• Compartmentalizing Cell-Free Systems: Toward Creating Life-Like Artificial Cells and Beyond.

  2020cites this paper
• Emerging Advances of Cell‐Free Systems toward Artificial Cells

  2020cites this paper
• A Sustainable and Efficient Artificial Microgel System: Toward Creating a Configurable Synthetic Cell.

  2020cites this paper
• Biotechnology and Bioengineering: Volume 117, Number 9, September 2020

  2020cites this paper
• Cell‐Free Protein Synthesis in Bifunctional Hyaluronan Microgels: A Strategy for In Situ Immobilization and Purification of His‐Tagged Proteins

  2019cites this paper
• Cell-free microcompartmentalised transcription–translation for the prototyping of synthetic communication networks

  2019cites this paper
• Branched DNA Architectures Produced by PCR‐Based Assembly as Gene Compartments for Cell‐Free Gene‐Expression Reactions

  2019cites this paper
• The Usual Suspects 2019: of Chips, Droplets, Synthesis, and Artificial Cells

  2019cites this paper
• Establishing communication between artificial cells.

  2019cites this paper
• Toward long-lasting artificial cells that better mimic natural living cells

  2019cites this paper
• Cell-free gene expression: an expanded repertoire of applications

  2019cites this paper
• Synthetic organelles.

  2019cites this paper
• Bottom-up construction of complex biological systems with cell-free synthetic biology

  2019cites this paper
• Microfluidic Droplets and Their Applications: Diagnosis, Drug Screening and the Discovery of Therapeutic Enzymes

  2018cites this paper
• Long-lived protein expression in hydrogel particles: towards artificial cells† †Electronic supplementary information (ESI) available: Experimental details and results. See DOI: 10.1039/c8sc00383a

  2018cites this paper
• Künstliche, gelbasierte Organellen für die räumliche Organisation von zellfreien Genexpressionsreaktionen

  2018cites this paper
• Synergistic interactions between confinement and macromolecular crowding spatially order transcription and translation in cell-free expression

  2018cites this paper
• Droplet-Assisted Microfluidic Fabrication and Characterization of Multifunctional Polysaccharide Microgels Formed by Multicomponent Reactions

  2018cites this paper
• Artificial Gel-Based Organelles for Spatial Organization of Cell-Free Gene Expression Reactions.

  2018cites this paper
• Microfluidic-Assisted Fabrication of Clay Microgels for Cell-Free Protein Synthesis.

  2018cites this paper
• Novel Aqueous Microgels and Their Applications

  2017cites this paper
• Mechanically Defined Microgels by Droplet Microfluidics

  2017cites this paper
• Polymer Material Design by Microfluidics Inspired by Cell Biology and Cell‐Free Biotechnology

  2017cites this paper
• Protein Synthesis in Coupled and Uncoupled Cell-Free Prokaryotic Gene Expression Systems.

  2016cites this paper
• Biocompatible fluorinated polyglycerols for droplet microfluidics as an alternative to PEG-based copolymer surfactants.

  2016cites this paper
• Discovery in Droplets.

  2016cites this paper
• Cell‐Like Nanostructured Environments Alter Diffusion and Reaction Kinetics in Cell‐Free Gene Expression

  2016cites this paper
• Towards a synthetic cell: localization, synchronization and noise in cell-free gene expression

  2015cites this paper
• Temperature-Invariant Aqueous Microgels as Hosts for Biomacromolecules.

  2015cites this paper
• Artificial microniches for probing mesenchymal stem cell fate in 3D.

  2014cites this paper
• Hydrogels for 3D mammalian cell culture: a starting guide for laboratory practice

  2014cites this paper