GTP hydrolysis controls stringent selection of the AUG start codon during translation initiation in Saccharomyces cerevisiae.

No abstract is available for this paper.

• Publication year

  1997

• Venue

  Genes & Development

• Publication date

  1997-09-15

• Fields of study

  Biology, Medicine

• Identifiers
  DOI 10.1101/GAD.11.18.2396PMID 9308967PMCID PMC316512
• External record

  Open on Semantic Scholar

• Source metadata

  Semantic Scholar, PubMed

• No claims are published for this paper.

• No concepts are published for this paper.

• Ligand interactions with eukaryotic translation initiation factor 2: role of the gamma‐subunit.

  1996cited by this paper
• SUI1/p16 is required for the activity of eukaryotic translation initiation factor 3 in Saccharomyces cerevisiae

  1996cited by this paper
• GTPase activity of Rab5 acts as a timer for endocytic membrane fusion

  1996cited by this paper
• Structural insights into the function of the Rab GDI superfamily.

  1996cited by this paper
• Crystal Structure of the Ternary Complex of Phe-tRNAPhe, EF-Tu, and a GTP Analog

  1995cited by this paper
• The amino acid sequence of eukaryotic translation initiation factor 1 and its similarity to yeast initiation factor SUI1

  1995cited by this paper
• Modulation of tRNA(iMet), eIF-2, and eIF-2B expression shows that GCN4 translation is inversely coupled to the level of eIF-2.GTP.Met-tRNA(iMet) ternary complexes

  1995cited by this paper
• Translation initiation factor eIF-2. Cloning and expression of the human cDNA encoding the gamma-subunit.

  1994cited by this paper
• Rab proteins in regulated exocytosis.

  1994cited by this paper
• Isolation and immunochemical characterization of eukaryotic translation initiation factor 5 from Saccharomyces cerevisiae.

  1993influential reference
• GCD11, a negative regulator of GCN4 expression, encodes the gamma subunit of eIF-2 in Saccharomyces cerevisiae

  1993influential reference
• Guide to yeast genetics and molecular biology

  1993cited by this paper
• Eukaryotic translation initiation factor 5 from Saccharomyces cerevisiae. Cloning, characterization, and expression of the gene encoding the 45,346-Da protein.

  1993cited by this paper
• A protein complex of translational regulators of GCN4 mRNA is the guanine nucleotide-exchange factor for translation initiation factor 2 in yeast.

  1993cited by this paper
• Guanine nucleotide exchange factor for eukaryotic translation initiation factor 2 in Saccharomyces cerevisiae: interactions between the essential subunits GCD2, GCD6, and GCD7 and the regulatory subunit GCN3

  1993cited by this paper
• Molecular cloning and expression of cDNA for mammalian translation initiation factor 5.

  1993cited by this paper
• Evidence that GCD6 and GCD7, translational regulators of GCN4, are subunits of the guanine nucleotide exchange factor for eIF-2 in Saccharomyces cerevisiae

  1993cited by this paper
• Mutation analysis of the Cys-X2-Cys-X19-Cys-X2-Cys motif in the beta subunit of eukaryotic translation initiation factor 2.

  1992cited by this paper
• Mechanism and regulation of eukaryotic protein synthesis.

  1992influential reference
• The suil suppressor locus in Saccharomyces cerevisiae encodes a translation factor that functions during tRNA(iMet) recognition of the start codon

  1992influential reference
• The GTPase superfamily: conserved structure and molecular mechanism

  1991influential reference
• Guide to yeast genetics and molecular biology.

  1991cited by this paper
• Translational control in mammalian cells.

  1991influential reference
• Function of eukaryotic initiation factor 5 in the formation of an 80 S ribosomal polypeptide chain initiation complex.

  1991cited by this paper
• Initiation of mRNA translation in prokaryotes.

  1990cited by this paper
• Genetic characterization of the Saccharomyces cerevisiae translational initiation suppressors sui1, sui2 and SUI3 and their effects on HIS4 expression.

  1990influential reference
• Small GTP-binding proteins in vesicular transport.

  1990cited by this paper
• Mutations in the structural genes for eukaryotic initiation factors 2 alpha and 2 beta of Saccharomyces cerevisiae disrupt translational control of GCN4 mRNA.

  1989cited by this paper
• A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

  1989cited by this paper
• The scanning model for translation: an update

  1989cited by this paper
• Yeast translation initiation suppressor sui2 encodes the alpha subunit of eukaryotic initiation factor 2 and shares sequence identity with the human alpha subunit.

  1989cited by this paper
• Context effects and inefficient initiation at non-AUG codons in eucaryotic cell-free translation systems

  1989cited by this paper
• Mutations at a Zn(II) finger motif in the yeast eIF-2 beta gene alter ribosomal start-site selection during the scanning process.

  1988cited by this paper
• Mutations at a Zn(II) finger motif in the yeast elF-2β gene alter ribosomal start-site selection during the scanning process

  1988influential reference
• A non-AUG translational initiation in c-myc exon 1 generates an N-terminally distinct protein whose synthesis is disrupted in Burkitt's lymphomas.

  1988cited by this paper
• Structure of the beta subunit of translational initiation factor eIF-2.

  1988cited by this paper
• Genetic selection for mutations that reduce or abolish ribosomal recognition of the HIS4 translational initiator region

  1988influential reference
• The effect of Mg2+ and guanine nucleotide exchange factor on the binding of guanine nucleotides to eukaryotic initiation factor 2.

  1988cited by this paper
• Mutational analysis of the HIS4 translational initiator region in Saccharomyces cerevisiae

  1988influential reference
• tRNAi(met) functions in directing the scanning ribosome to the start site of translation.

  1988influential reference
• EFTu provides an internal kinetic standard for translational accuracy.

  1988cited by this paper
• The reaction of ribosomes with elongation factor Tu.GTP complexes. Aminoacyl-tRNA-independent reactions in the elongation cycle determine the accuracy of protein synthesis.

  1986cited by this paper
• Vector systems for the expression, analysis and cloning of DNA sequence in S. cerevisiae

  1985influential reference
• One-step gene disruption in yeast.

  1983cited by this paper
• Genetic applications of yeast transformation with linear and gapped plasmids.

  1983cited by this paper
• The nucleotide sequence of the HIS4 region of yeast.

  1982influential reference
• Characterization of eukaryotic initiation factor 2 containing two polypeptide chains of Mr = 48,000 and 38,000.

  1981cited by this paper
• DNA rearrangements associated with a transposable element in yeast.

  1980cited by this paper
• Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.

  1979cited by this paper
• Role of eukaryotic initiation factor 5 in the formation of 80 S initiation complexes.

  1979cited by this paper
• The ribosomal proteins of Saccharomyces cerevisiae.

  1978cited by this paper
• Chapter 4 The Ribosomal Proteins of Saccharomyces cerevisiae

  1978cited by this paper
• How do eucaryotic ribosomes select initiation regions in messenger RNA?

  1978cited by this paper
• DNA sequencing with chain-terminating inhibitors.

  1977cited by this paper
• Soluble protein factors and ribosomal subunits from yeast. Interactions with aminoacyl-tRNA.

  1974cited by this paper
• Characterization of the ribosome-dependent guanosine triphosphatase activity of polypeptide chain initiation factor IF 2.

  1972cited by this paper

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  2025cites this paper
• eIF1 and eIF5 dynamically control translation start site fidelity

  2024cites this paper
• Structural basis of AUC codon discrimination during translation initiation in yeast

  2024cites this paper
• eIF2β zinc-binding domain interacts with the eIF2γ subunit through the guanine nucleotide binding interface to promote Met-tRNAiMet binding

  2024cites this paper
• Recognition of γ-subunit by β-subunit. Stabilization of the GTP-bound state of translation initiation factor 2 in archaea and eukaryotes

  2023cites this paper
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  2023cites this paper
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  2023cites this paper
• Translational fidelity screens in mammalian cells reveal eIF3 and eIF4G2 as regulators of start codon selectivity

  2023cites this paper
• Wide mutational analysis to ascertain the functional roles of eL33 in ribosome biogenesis and translation initiation

  2022cites this paper
• Non-AUG translation initiation in mammals

  2022cites this paper
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  2022cites this paper
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  2019cites this paper
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  2014cites this paper
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